通过构建体外口腔微观世界模型,运用结晶紫染色法、总菌落形成单位(CFU)计数法、聚合酶链式反应(PCR)技术、乳酸浓度测定和16S r DNA测序等方法,探究了后生元牙膏及牙膏中的十二烷基硫酸钠(SLS)对口腔生物膜抗龋性的影响。结果表明,后...通过构建体外口腔微观世界模型,运用结晶紫染色法、总菌落形成单位(CFU)计数法、聚合酶链式反应(PCR)技术、乳酸浓度测定和16S r DNA测序等方法,探究了后生元牙膏及牙膏中的十二烷基硫酸钠(SLS)对口腔生物膜抗龋性的影响。结果表明,后生元牙膏(PT)干预能够维持健康生物膜的正常生长和代谢,致龋挑战后,PT组生物膜活菌数、细菌总量和代谢活性均较低,抗龋性好。SLS能够破坏健康生物膜的生长、代谢和三维网状结构,致龋挑战后,SLS组生物膜的生物量、活菌数、细菌总量和代谢活性升高,龋病的发生风险升高。生物膜群落分析发现,PT组α多样性和菌群结构均与阴性(超纯水)对照组接近,且在致龋挑战后保持较好的稳定性。SLS组具有较高的α多样性,但是其菌群结构失调,且不稳定。PT和SLS联合干预组的结果显示,由于PT能够有效对抗SLS对生物膜的破坏作用,因此后生元牙膏中的SLS并不足以引起口腔微生态失调。本研究可为口腔护理产品有效成分的开发提供参考。展开更多
Hyperactivity and tic symptoms in children and adolescents with Attention Deficit Hyperactivity Disorder(ADHD)have a bidirectional association with sleep disorders,while magnesium deficiency is a key nutritional facto...Hyperactivity and tic symptoms in children and adolescents with Attention Deficit Hyperactivity Disorder(ADHD)have a bidirectional association with sleep disorders,while magnesium deficiency is a key nutritional factor exacerbating this vicious cycle.The SLS Magnesium Glycinate Complex Liquid Supplement formula adopts the core logic of“sleep improvement-mood stabilization-behavioral regulation”and enhances the neuromodulatory effects of magnesium ions through multi-component synergy.Tailored to the developmental needs of different stages aged 4-16 years,the formula features a child-friendly dosage design,forming a closed-loop regulation in aiding sleep,reducing tics,and maintaining daytime functioning.A 3-month clinical trial showed that the formula improved sleep efficiency by 15%-23%,reduced tic frequency by 21%-32%,and enhanced emotional stability scores by 24%-30%across all age groups with ADHD,with good safety and tolerability.This study provides a targeted nutritional intervention solution for ADHD,and its age-stratified design concept offers scientific reference for precise nutritional support during different developmental stages of children and adolescents.展开更多
文摘通过构建体外口腔微观世界模型,运用结晶紫染色法、总菌落形成单位(CFU)计数法、聚合酶链式反应(PCR)技术、乳酸浓度测定和16S r DNA测序等方法,探究了后生元牙膏及牙膏中的十二烷基硫酸钠(SLS)对口腔生物膜抗龋性的影响。结果表明,后生元牙膏(PT)干预能够维持健康生物膜的正常生长和代谢,致龋挑战后,PT组生物膜活菌数、细菌总量和代谢活性均较低,抗龋性好。SLS能够破坏健康生物膜的生长、代谢和三维网状结构,致龋挑战后,SLS组生物膜的生物量、活菌数、细菌总量和代谢活性升高,龋病的发生风险升高。生物膜群落分析发现,PT组α多样性和菌群结构均与阴性(超纯水)对照组接近,且在致龋挑战后保持较好的稳定性。SLS组具有较高的α多样性,但是其菌群结构失调,且不稳定。PT和SLS联合干预组的结果显示,由于PT能够有效对抗SLS对生物膜的破坏作用,因此后生元牙膏中的SLS并不足以引起口腔微生态失调。本研究可为口腔护理产品有效成分的开发提供参考。
文摘Hyperactivity and tic symptoms in children and adolescents with Attention Deficit Hyperactivity Disorder(ADHD)have a bidirectional association with sleep disorders,while magnesium deficiency is a key nutritional factor exacerbating this vicious cycle.The SLS Magnesium Glycinate Complex Liquid Supplement formula adopts the core logic of“sleep improvement-mood stabilization-behavioral regulation”and enhances the neuromodulatory effects of magnesium ions through multi-component synergy.Tailored to the developmental needs of different stages aged 4-16 years,the formula features a child-friendly dosage design,forming a closed-loop regulation in aiding sleep,reducing tics,and maintaining daytime functioning.A 3-month clinical trial showed that the formula improved sleep efficiency by 15%-23%,reduced tic frequency by 21%-32%,and enhanced emotional stability scores by 24%-30%across all age groups with ADHD,with good safety and tolerability.This study provides a targeted nutritional intervention solution for ADHD,and its age-stratified design concept offers scientific reference for precise nutritional support during different developmental stages of children and adolescents.
