Plant root stem cells and their surrounding microenvironment,namely the stem cell niche,are hypersensitive to DNA damage.However,the molecular mechanisms that help maintain the genome stability of root stem cells rema...Plant root stem cells and their surrounding microenvironment,namely the stem cell niche,are hypersensitive to DNA damage.However,the molecular mechanisms that help maintain the genome stability of root stem cells remain elusive.Here we show that the root stem cells in the skbl（Shk1 kinase binding protein 1） mutant undergoes DNA damage-induced cell death,which is enhanced when combined with a lesion of the Ataxia-telangiectasia mutated（ATM） or the ATM/RAD3-related（ATR） genes,suggesting that the SKBI plays a synergistically effect with ATM and ATR in DNA damage pathway.We also provide evidence that SKBI is required for the maintenance of quiescent center（QC）,a root stem cell niche,under DNA damage treatments.Furthermore,we report decreased and ectopic expression of SHORTROOT（SHR） in response to DNA damage in the skbl root tips,while the expression of SCARECROW（SCR） remains unaffected.Our results uncover a new mechanism of plant root stem cell maintenance under DNA damage conditions that requires SKB1.展开更多
The demands of programmability have become more and more exigent as novel network services appear, such as E-commerce, social softwares, and online videos. Commodity multi-core CPUs have been widely applied in network...The demands of programmability have become more and more exigent as novel network services appear, such as E-commerce, social softwares, and online videos. Commodity multi-core CPUs have been widely applied in network packet processing to get high programmability and reduce the time-to-market. However,there is a great gap between the packet processing performance of commodity multi-core and that of the traditional packet processing hardware, e.g., NP(Network Process). Recently, optimization of the packet processing performance of commodity multi-cores has become a hot topic in industry and academia. In this paper, based on a detailed analysis of the packet processing procedure, firstly we identify two dominating overheads, namely the virtual-to-physical address translation and the packet buffer management. Secondly, we make a comprehensive survey on the current optimization methods. Thirdly, based on the survey, the heterogeneous architecture of the commodity multi-core + FPGA is proposed as a promising way to improve the packet processing performance.Fourthly, a novel Self-Described Buffer(SDB) management technology is introduced to eliminate the overheads of the allocation and deallocation of the packet buffers offloaded to FPGA. Then, an evaluation testbed, named PIOT(Packet I/O Testbed), is designed and implemented to evaluate the packet forwarding performance. I/O capacity of different commodity multi-core CPUs and the performance of optimization methods are assessed and compared based on PIOT. At last, the future work of packet processing optimization on multi-core CPUs is discussed.展开更多
文摘Plant root stem cells and their surrounding microenvironment,namely the stem cell niche,are hypersensitive to DNA damage.However,the molecular mechanisms that help maintain the genome stability of root stem cells remain elusive.Here we show that the root stem cells in the skbl（Shk1 kinase binding protein 1） mutant undergoes DNA damage-induced cell death,which is enhanced when combined with a lesion of the Ataxia-telangiectasia mutated（ATM） or the ATM/RAD3-related（ATR） genes,suggesting that the SKBI plays a synergistically effect with ATM and ATR in DNA damage pathway.We also provide evidence that SKBI is required for the maintenance of quiescent center（QC）,a root stem cell niche,under DNA damage treatments.Furthermore,we report decreased and ectopic expression of SHORTROOT（SHR） in response to DNA damage in the skbl root tips,while the expression of SCARECROW（SCR） remains unaffected.Our results uncover a new mechanism of plant root stem cell maintenance under DNA damage conditions that requires SKB1.
基金supported by National High-tech R&D Program of China(863 Program)(Grant No.2015AA0156-03)National Natural Science Foundation of China(Grant No.61202483)
文摘The demands of programmability have become more and more exigent as novel network services appear, such as E-commerce, social softwares, and online videos. Commodity multi-core CPUs have been widely applied in network packet processing to get high programmability and reduce the time-to-market. However,there is a great gap between the packet processing performance of commodity multi-core and that of the traditional packet processing hardware, e.g., NP(Network Process). Recently, optimization of the packet processing performance of commodity multi-cores has become a hot topic in industry and academia. In this paper, based on a detailed analysis of the packet processing procedure, firstly we identify two dominating overheads, namely the virtual-to-physical address translation and the packet buffer management. Secondly, we make a comprehensive survey on the current optimization methods. Thirdly, based on the survey, the heterogeneous architecture of the commodity multi-core + FPGA is proposed as a promising way to improve the packet processing performance.Fourthly, a novel Self-Described Buffer(SDB) management technology is introduced to eliminate the overheads of the allocation and deallocation of the packet buffers offloaded to FPGA. Then, an evaluation testbed, named PIOT(Packet I/O Testbed), is designed and implemented to evaluate the packet forwarding performance. I/O capacity of different commodity multi-core CPUs and the performance of optimization methods are assessed and compared based on PIOT. At last, the future work of packet processing optimization on multi-core CPUs is discussed.
