食管癌的发生、发展与许多因素有关,其中免疫系统发挥重要作用。CD47-SIRPα是PD-1/PD-L1,CTLA4之后的又一个免疫检查点,已经证明在多种血液系统肿瘤与实体瘤中高表达,通过产生抑制性信号阻止巨噬细胞吞噬癌细胞。本文针对其在食管癌中...食管癌的发生、发展与许多因素有关,其中免疫系统发挥重要作用。CD47-SIRPα是PD-1/PD-L1,CTLA4之后的又一个免疫检查点,已经证明在多种血液系统肿瘤与实体瘤中高表达,通过产生抑制性信号阻止巨噬细胞吞噬癌细胞。本文针对其在食管癌中的表达、与患者预后的关系、免疫治疗进行综述,通过本文的阐述,食管癌中的CD47-SIRPα同样是高表达,高CD47或高SIRPα都是导致食管癌患者预后不良的因素,抗CD47抗体与人源性食管癌细胞、人源性食管癌裸鼠模型有一定的结合活性。The occurrence and development of esophageal cancer are related to many factors, among which the immune system plays an important role. CD47-SIRPα is another immune checkpoint after PD-1/PD-L1 and CTLA4. It has been proved to be highly expressed in various hematological malignancies and solid tumors. It inhibits macrophage phagocytosis of cancer cells by generating inhibitory signals. This article reviews the expression of CD47-SIRPα in esophageal cancer, its relationship with the prognosis of patients, and immunotherapy. Through the elaboration of this article, CD47-SIRPα is also highly expressed in esophageal cancer. High CD47 or high SIRPα are both factors contributing to poor prognosis of patients with esophageal cancer, Anti-CD47 antibodies have certain binding activity with human esophageal cancer cells and human esophageal cancer nude mouse models.展开更多
目的:研究信号调节蛋白α(signal regulatory protein α,SIRPα)对乳腺癌细胞黏附、侵袭和凋亡的影响及其可能机制。方法:Western blotting检测侵袭能力强的MDA-MB-231乳腺癌细胞和侵袭能力弱的MDA-MB-435乳腺癌细胞中SIRPα蛋白的表...目的:研究信号调节蛋白α(signal regulatory protein α,SIRPα)对乳腺癌细胞黏附、侵袭和凋亡的影响及其可能机制。方法:Western blotting检测侵袭能力强的MDA-MB-231乳腺癌细胞和侵袭能力弱的MDA-MB-435乳腺癌细胞中SIRPα蛋白的表达。脂质体法将pcDNA3.0-SIRPα转染MDA-MB-231细胞后,RT-PCR检测MDA-MB-231细胞SIRPαmRNA的表达,TUNEL法检测细胞的凋亡,细胞侵袭实验观察细胞侵袭能力变化,黏附实验观察细胞黏附能力变化,Westernblotting检测JNK和p-JNK蛋白的表达。EGF刺激MDA-MB-435细胞,免疫共沉淀检测MDA-MB-435细胞中SIRPα与SHP2的结合。结果:侵袭能力强的MDA-MB-231细胞不表达SIRPα,侵袭能力弱的MDA-MB-435细胞表达高水平SIRPα蛋白。pcDNA3.0-SIRPα转染可增强MDA-MB-231细胞的黏附,降低MDA-MB-231细胞的侵袭能力,并促进MDA-MB-231细胞的凋亡。pcDNA3.0-SIRPα转染抑制MDA-MB-231细胞JNK的磷酸化。EGF刺激可进一步上调MDA-MB-435细胞中SIRPα蛋白表达,并促进SIRPα与SHP-2蛋白的结合。结论:SIRPα与乳腺癌细胞的黏附、侵袭能力相关,并可能通过抑制JNK磷酸化促进乳腺癌细胞凋亡。展开更多
文摘食管癌的发生、发展与许多因素有关,其中免疫系统发挥重要作用。CD47-SIRPα是PD-1/PD-L1,CTLA4之后的又一个免疫检查点,已经证明在多种血液系统肿瘤与实体瘤中高表达,通过产生抑制性信号阻止巨噬细胞吞噬癌细胞。本文针对其在食管癌中的表达、与患者预后的关系、免疫治疗进行综述,通过本文的阐述,食管癌中的CD47-SIRPα同样是高表达,高CD47或高SIRPα都是导致食管癌患者预后不良的因素,抗CD47抗体与人源性食管癌细胞、人源性食管癌裸鼠模型有一定的结合活性。The occurrence and development of esophageal cancer are related to many factors, among which the immune system plays an important role. CD47-SIRPα is another immune checkpoint after PD-1/PD-L1 and CTLA4. It has been proved to be highly expressed in various hematological malignancies and solid tumors. It inhibits macrophage phagocytosis of cancer cells by generating inhibitory signals. This article reviews the expression of CD47-SIRPα in esophageal cancer, its relationship with the prognosis of patients, and immunotherapy. Through the elaboration of this article, CD47-SIRPα is also highly expressed in esophageal cancer. High CD47 or high SIRPα are both factors contributing to poor prognosis of patients with esophageal cancer, Anti-CD47 antibodies have certain binding activity with human esophageal cancer cells and human esophageal cancer nude mouse models.
文摘目的:研究信号调节蛋白α(signal regulatory protein α,SIRPα)对乳腺癌细胞黏附、侵袭和凋亡的影响及其可能机制。方法:Western blotting检测侵袭能力强的MDA-MB-231乳腺癌细胞和侵袭能力弱的MDA-MB-435乳腺癌细胞中SIRPα蛋白的表达。脂质体法将pcDNA3.0-SIRPα转染MDA-MB-231细胞后,RT-PCR检测MDA-MB-231细胞SIRPαmRNA的表达,TUNEL法检测细胞的凋亡,细胞侵袭实验观察细胞侵袭能力变化,黏附实验观察细胞黏附能力变化,Westernblotting检测JNK和p-JNK蛋白的表达。EGF刺激MDA-MB-435细胞,免疫共沉淀检测MDA-MB-435细胞中SIRPα与SHP2的结合。结果:侵袭能力强的MDA-MB-231细胞不表达SIRPα,侵袭能力弱的MDA-MB-435细胞表达高水平SIRPα蛋白。pcDNA3.0-SIRPα转染可增强MDA-MB-231细胞的黏附,降低MDA-MB-231细胞的侵袭能力,并促进MDA-MB-231细胞的凋亡。pcDNA3.0-SIRPα转染抑制MDA-MB-231细胞JNK的磷酸化。EGF刺激可进一步上调MDA-MB-435细胞中SIRPα蛋白表达,并促进SIRPα与SHP-2蛋白的结合。结论:SIRPα与乳腺癌细胞的黏附、侵袭能力相关,并可能通过抑制JNK磷酸化促进乳腺癌细胞凋亡。
