OBJECTIVE: To investigate composition of gut microbial community in a rat model of functional dyspepsia(FD) and to explore the interventional effects of Simo Tang(四磨汤, SMT). METHODS: A rat model of FD was establish...OBJECTIVE: To investigate composition of gut microbial community in a rat model of functional dyspepsia(FD) and to explore the interventional effects of Simo Tang(四磨汤, SMT). METHODS: A rat model of FD was established through the tail-clamping stimulation method. The rat model of FD was assessed by the state of rats, their weight, sucrose preference rate, and intestinal propulsion rate. The DNA was extracted from stool samples after treatment with SMT. Amplified polymerase chain reaction(PCR) products of the 16S r DNA were sequenced using Novase Q6000 after construction of libraries. Composition of gut microbial community in the stool samples was determined and analyzed by cluster analysis, bioinformatic analysis, and analysis of α-diversity and β-diversity.RESULTS: The rat model of FD was successfully established using the tail-clamping stimulation method. The statistical results of cluster analysis of operational taxonomic units(OTUs) showed that the relative abundance of OTUs in the FD group was the lowest, while it was the highest in the normal(N) group. The composition of microbiome in the four groups was similar at phyla level. Compared with the FD group, the abundance of Firmicutes was downregulated, and the abundance of Proteobacteria and Bacteroidetes was upregulated in the Simo Tang(SMT) and high-dose Simo Tang(SMT.G) groups. The ratio of Bacteroidetes/Firmicutes was also elevated. According to the analysis of α-diversity and β-diversity, the abundance of flora in FD rats was significantly reduced. The treatment using SMT appeared beneficial to improve the diversity of flora. SMT could improve the intestinal flora in FD rats. The results showed that FD rats had intestinal flora imbalance, and species diversity increased. The results suggested that SMT could regulate the disorders of intestinal flora caused by FD. CONCLUDIONS: SMT could restore gut homeostasis and maintain gut flora diversity by modulating the gut microbiota and its associated metabolites in rats, thereby treating gastrointestinal diseases.展开更多
The present work aims to investigate the effect of heating temperature(400,600 and 800°C)and inoculating elements(Ca,Ca-Ba,Ca-RE)on oxidation behavior of ductile irons containing 5.25%Si and 4.8%Si-2.3%Mo in dry ...The present work aims to investigate the effect of heating temperature(400,600 and 800°C)and inoculating elements(Ca,Ca-Ba,Ca-RE)on oxidation behavior of ductile irons containing 5.25%Si and 4.8%Si-2.3%Mo in dry air and combustion gas containing water vapour(natural gas burning).The oxidation is influenced by the gas atmosphere type,the iron alloying system,and the inoculating elements depending on the heating temperature.The weight gain increases from 0.001%-0.1%(400°C)to 0.05%-0.70%(600°C)and up to 0.10%-2.15%(800°C).No particular effects of the considered influencing factors are found when heating at 400°C,while at 600°C,mainly the oxidation gas atmosphere type shows a visible influence.At the highest heating temperature of 800°C,a limited increase of the weight gain is found for dry air atmosphere(up to 0.25%),but it drastically increases for combustion atmospheres(0.65%-2.15%).The water vapour presence in the combustion atmosphere is an important oxidising factor at 600-800°C.The alloying system appears to influence the oxidation behavior mainly at a heating temperature of 800°C in the combustion atmosphere,as evidenced by the lower weight gain in 5.25%silicon cast iron.Positive effects of inoculating elements increase with the heating temperature,with Ca and Ba-FeSi inoculation generally showing better performance.Irons inoculated with CaRE-FeSi exhibit a higher degree of oxidation.These results are in good relationship with the previous reported data:Ca-Ba-inoculation system appears to be better than simple Ca for improving the graphite parameters,while RE-bearing inoculant negatively affects the compactness degree of graphite particles in high-Si ductile irons.As the lower compactness degree is typical for graphite nodules in high-Si ductile irons,which negatively affects the oxidation resistance,it is necessary to employ specific metallurgical treatments to improve nodule quality.Inoculation,in particular,is a potential method to achieve this improvement.展开更多
ZHOU Bai-Bin *,1,2 WEI Yong-De 1 LI Zhong-Hua 1 ( 1 Department of Applied Chemistry,Ha rbin Institute of Technology ￡?Harbin￡±￡ì￡°￡°￡°￡±￡(c)( 2 Department of Chemistry,Harbin Norm...ZHOU Bai-Bin *,1,2 WEI Yong-De 1 LI Zhong-Hua 1 ( 1 Department of Applied Chemistry,Ha rbin Institute of Technology ￡?Harbin￡±￡ì￡°￡°￡°￡±￡(c)( 2 Department of Chemistry,Harbin Normal University￡?Harbin￡±￡ì￡°￡°￡?￡°￡(c) The air-solid interface reaction of Ce,Lu with K10 H 3[Gd (SiMo 4 W￡*O￡3￡1￡(c) 2]through chemistry-heated permeation is reported for the fi rst time￡(r)The permeated complex is characterized by ICP and the result shows tha t the mini mum Ce,Lu can permeate into the inner sph ere of K 10 H ￡3 ￡?Gd ￡¨SiMo ￡′ W ￡* O ￡3￡1 ￡(c) 2]The IR ,XRD patterns give the eviden ce that after permeation the comple x still keeps the Keggin structure,howe ver,its crystal structure is different from the complex before permeation￡(r)The cond uctivity of the permeated complex has been measured with the four-electr ode method and the data show that the co nductivity of the complex after permeation is 10 6 times higher than that of the sample before permeation and reaches ￡′￡(r)84 6×10 -1 S·cm -1 ￡(r)These indicate that the permeated c omplex is a good solid electrolyte and further appli cations are also expected￡(r)展开更多
针对恒包络信号在时变平坦衰落单输入多输出(SIMO)信道下的信噪比盲估计问题,提出一种基于 EM(Expectation-Maximization)算法的改进 M2M4-EM 算法。利用 EM 算法的迭代期望值最大化思想,推导了 SIMO 信道下 EM 算法的闭式解。为进一步...针对恒包络信号在时变平坦衰落单输入多输出(SIMO)信道下的信噪比盲估计问题,提出一种基于 EM(Expectation-Maximization)算法的改进 M2M4-EM 算法。利用 EM 算法的迭代期望值最大化思想,推导了 SIMO 信道下 EM 算法的闭式解。为进一步改善性能,通过接收信号的二阶和四阶矩(M2M4)对 EM 算法进行初始化。仿真结果表明, M2M4-EM 算法在较宽的信噪比范围内具有较好的估计精度和较快的收敛速度。展开更多
基金National Natural Science Foundation of China:Changes of Ras Homolog Gene Family Member A/Rho-associated Coiled-coil Kinase Signaling Pathway in Liver-Stomach Disharmony Functional Dyspepsia Rats and Intervention Mechanism of Simo Tang (No. 81803896)the Role of Gut Microbiota-diaminopimelic Acid Nucleotide-binding and Oligomerization Domain1-receptorinteracting Protein 2 Signal Pathway in Severe Process of Acute Pancreatitis and the Intervention Mechanism of Qingxia Therapy (No. 81873156)Research Project of Liaoning Provincial Department of Education:the Role of Mitochondria-Derived Reactive Oxygen Species Mediated Autophagy Activation in Myocardial Injury in Sepsis (No. LZ2020036)。
文摘OBJECTIVE: To investigate composition of gut microbial community in a rat model of functional dyspepsia(FD) and to explore the interventional effects of Simo Tang(四磨汤, SMT). METHODS: A rat model of FD was established through the tail-clamping stimulation method. The rat model of FD was assessed by the state of rats, their weight, sucrose preference rate, and intestinal propulsion rate. The DNA was extracted from stool samples after treatment with SMT. Amplified polymerase chain reaction(PCR) products of the 16S r DNA were sequenced using Novase Q6000 after construction of libraries. Composition of gut microbial community in the stool samples was determined and analyzed by cluster analysis, bioinformatic analysis, and analysis of α-diversity and β-diversity.RESULTS: The rat model of FD was successfully established using the tail-clamping stimulation method. The statistical results of cluster analysis of operational taxonomic units(OTUs) showed that the relative abundance of OTUs in the FD group was the lowest, while it was the highest in the normal(N) group. The composition of microbiome in the four groups was similar at phyla level. Compared with the FD group, the abundance of Firmicutes was downregulated, and the abundance of Proteobacteria and Bacteroidetes was upregulated in the Simo Tang(SMT) and high-dose Simo Tang(SMT.G) groups. The ratio of Bacteroidetes/Firmicutes was also elevated. According to the analysis of α-diversity and β-diversity, the abundance of flora in FD rats was significantly reduced. The treatment using SMT appeared beneficial to improve the diversity of flora. SMT could improve the intestinal flora in FD rats. The results showed that FD rats had intestinal flora imbalance, and species diversity increased. The results suggested that SMT could regulate the disorders of intestinal flora caused by FD. CONCLUDIONS: SMT could restore gut homeostasis and maintain gut flora diversity by modulating the gut microbiota and its associated metabolites in rats, thereby treating gastrointestinal diseases.
基金supported by a grant from National Program for Research of the National Association of Technical Universities-GNAC ARUT 2023.
文摘The present work aims to investigate the effect of heating temperature(400,600 and 800°C)and inoculating elements(Ca,Ca-Ba,Ca-RE)on oxidation behavior of ductile irons containing 5.25%Si and 4.8%Si-2.3%Mo in dry air and combustion gas containing water vapour(natural gas burning).The oxidation is influenced by the gas atmosphere type,the iron alloying system,and the inoculating elements depending on the heating temperature.The weight gain increases from 0.001%-0.1%(400°C)to 0.05%-0.70%(600°C)and up to 0.10%-2.15%(800°C).No particular effects of the considered influencing factors are found when heating at 400°C,while at 600°C,mainly the oxidation gas atmosphere type shows a visible influence.At the highest heating temperature of 800°C,a limited increase of the weight gain is found for dry air atmosphere(up to 0.25%),but it drastically increases for combustion atmospheres(0.65%-2.15%).The water vapour presence in the combustion atmosphere is an important oxidising factor at 600-800°C.The alloying system appears to influence the oxidation behavior mainly at a heating temperature of 800°C in the combustion atmosphere,as evidenced by the lower weight gain in 5.25%silicon cast iron.Positive effects of inoculating elements increase with the heating temperature,with Ca and Ba-FeSi inoculation generally showing better performance.Irons inoculated with CaRE-FeSi exhibit a higher degree of oxidation.These results are in good relationship with the previous reported data:Ca-Ba-inoculation system appears to be better than simple Ca for improving the graphite parameters,while RE-bearing inoculant negatively affects the compactness degree of graphite particles in high-Si ductile irons.As the lower compactness degree is typical for graphite nodules in high-Si ductile irons,which negatively affects the oxidation resistance,it is necessary to employ specific metallurgical treatments to improve nodule quality.Inoculation,in particular,is a potential method to achieve this improvement.
文摘ZHOU Bai-Bin *,1,2 WEI Yong-De 1 LI Zhong-Hua 1 ( 1 Department of Applied Chemistry,Ha rbin Institute of Technology ￡?Harbin￡±￡ì￡°￡°￡°￡±￡(c)( 2 Department of Chemistry,Harbin Normal University￡?Harbin￡±￡ì￡°￡°￡?￡°￡(c) The air-solid interface reaction of Ce,Lu with K10 H 3[Gd (SiMo 4 W￡*O￡3￡1￡(c) 2]through chemistry-heated permeation is reported for the fi rst time￡(r)The permeated complex is characterized by ICP and the result shows tha t the mini mum Ce,Lu can permeate into the inner sph ere of K 10 H ￡3 ￡?Gd ￡¨SiMo ￡′ W ￡* O ￡3￡1 ￡(c) 2]The IR ,XRD patterns give the eviden ce that after permeation the comple x still keeps the Keggin structure,howe ver,its crystal structure is different from the complex before permeation￡(r)The cond uctivity of the permeated complex has been measured with the four-electr ode method and the data show that the co nductivity of the complex after permeation is 10 6 times higher than that of the sample before permeation and reaches ￡′￡(r)84 6×10 -1 S·cm -1 ￡(r)These indicate that the permeated c omplex is a good solid electrolyte and further appli cations are also expected￡(r)
文摘针对恒包络信号在时变平坦衰落单输入多输出(SIMO)信道下的信噪比盲估计问题,提出一种基于 EM(Expectation-Maximization)算法的改进 M2M4-EM 算法。利用 EM 算法的迭代期望值最大化思想,推导了 SIMO 信道下 EM 算法的闭式解。为进一步改善性能,通过接收信号的二阶和四阶矩(M2M4)对 EM 算法进行初始化。仿真结果表明, M2M4-EM 算法在较宽的信噪比范围内具有较好的估计精度和较快的收敛速度。
