Fusarium head blight(FHB),mainly caused by fungus Fusarium graminearum,is a devastating wheat disease worldwide,leading to reduced yield production and compromised grain quality due to contamination by mycotoxins,such...Fusarium head blight(FHB),mainly caused by fungus Fusarium graminearum,is a devastating wheat disease worldwide,leading to reduced yield production and compromised grain quality due to contamination by mycotoxins,such as deoxynivalenol(DON).Manipulating the specific gene expression in microorganisms through RNA interference(RNAi)presents an opportunity for new-generation double-stranded RNA(dsRNA)-based formulations to combat a large number of plant diseases.Here,we applied both spray-induced gene silencing(SIGS)and host-induced gene silencing(HIGS)to target five virulence-related and DON-synthesized genes in F.graminearum,including protein kinase gene Gpmk1,zinc finger protein gene Fg Chy1,transcription factor Fg SR,DON synthesis gene TRI5 and the cell-end marker protein gene Fg Tea A,aiming to effectively control FHB in wheat.Direct spraying of individual or combined small interfering RNA(siRNAs)from the fungus showed reduced expression of target genes and suppressed pathogenic symptoms during F.graminearum infection in wheat leaves,with the combination of all five siRNAs demonstrating superior resistance.Furthermore,we generated transgenic wheat lines expressing chimeric RNAi cassettes targeting these five genes,and two independent lines exhibited strong resistance to FHB and Fusarium crown rot,and the reduced DON accumulation.Notably,the HIGS transgenic lines did not adversely impact plant growth and yield traits.Collectively,our findings support that SIGS and HIGS represent effective strategies targeting key pathogenic genes for bolstering disease resistance in crops.展开更多
针对当前基于Web服务的空间信息格网(Spatial Information Grid,SIG)系统无法获取资源中间状态的缺点,探讨了一种基于Web服务资源框架(Web Service Resource Framework,WSRF)构建SIG的实现技术,而使SIG成为一种能够提供资源中间状态(sta...针对当前基于Web服务的空间信息格网(Spatial Information Grid,SIG)系统无法获取资源中间状态的缺点,探讨了一种基于Web服务资源框架(Web Service Resource Framework,WSRF)构建SIG的实现技术,而使SIG成为一种能够提供资源中间状态(stateful)的系统,有利于客户端与服务端的持久交互和状态回溯.在介绍了WSRF框架及其实现方式的基础上,探讨了基于WSRF构建空间信息服务的流程和关键,即确定系统中状态化的资源种类和空间信息服务.以地理坐标转换为例示范了状态化服务资源的创建过程,并提出了基于WSRF的SIG服务框架.展开更多
随着GIS的普及和深化,空间信息的大数据量、高复杂性和难共享的问题对其研究和应用提出了新的挑战。空间信息网格 (Spatial Information Grid,SIG)作为创新性的网络空间信息基础设施和技术体系,为解决这些问题提供了新的契机。本文在给...随着GIS的普及和深化,空间信息的大数据量、高复杂性和难共享的问题对其研究和应用提出了新的挑战。空间信息网格 (Spatial Information Grid,SIG)作为创新性的网络空间信息基础设施和技术体系,为解决这些问题提供了新的契机。本文在给出SIG基本定义和基本特征的基础上,就如何实现基于SIG的矿产资源评价进行了有益的探索和实践,并取得了很好的效果,为矿产资源评价提供了新的方法和思路。展开更多
基金financially supported by the National Key R&D Program of China(2022YFD1400105)the Jiangsu Agricultural Science and Technology Innovation Fund(CX(22)2005)+3 种基金the Jiangsu Key R&D Plan(Modern Agriculture),China(BE2022346)the China Agricultural Research System Program(CARS-03)the National Science Fund for Excellent Young Scholars(Overseas),Chinathe Start-Up Grant from Nanjing Agricultural University,China。
文摘Fusarium head blight(FHB),mainly caused by fungus Fusarium graminearum,is a devastating wheat disease worldwide,leading to reduced yield production and compromised grain quality due to contamination by mycotoxins,such as deoxynivalenol(DON).Manipulating the specific gene expression in microorganisms through RNA interference(RNAi)presents an opportunity for new-generation double-stranded RNA(dsRNA)-based formulations to combat a large number of plant diseases.Here,we applied both spray-induced gene silencing(SIGS)and host-induced gene silencing(HIGS)to target five virulence-related and DON-synthesized genes in F.graminearum,including protein kinase gene Gpmk1,zinc finger protein gene Fg Chy1,transcription factor Fg SR,DON synthesis gene TRI5 and the cell-end marker protein gene Fg Tea A,aiming to effectively control FHB in wheat.Direct spraying of individual or combined small interfering RNA(siRNAs)from the fungus showed reduced expression of target genes and suppressed pathogenic symptoms during F.graminearum infection in wheat leaves,with the combination of all five siRNAs demonstrating superior resistance.Furthermore,we generated transgenic wheat lines expressing chimeric RNAi cassettes targeting these five genes,and two independent lines exhibited strong resistance to FHB and Fusarium crown rot,and the reduced DON accumulation.Notably,the HIGS transgenic lines did not adversely impact plant growth and yield traits.Collectively,our findings support that SIGS and HIGS represent effective strategies targeting key pathogenic genes for bolstering disease resistance in crops.
文摘针对当前基于Web服务的空间信息格网(Spatial Information Grid,SIG)系统无法获取资源中间状态的缺点,探讨了一种基于Web服务资源框架(Web Service Resource Framework,WSRF)构建SIG的实现技术,而使SIG成为一种能够提供资源中间状态(stateful)的系统,有利于客户端与服务端的持久交互和状态回溯.在介绍了WSRF框架及其实现方式的基础上,探讨了基于WSRF构建空间信息服务的流程和关键,即确定系统中状态化的资源种类和空间信息服务.以地理坐标转换为例示范了状态化服务资源的创建过程,并提出了基于WSRF的SIG服务框架.
文摘随着GIS的普及和深化,空间信息的大数据量、高复杂性和难共享的问题对其研究和应用提出了新的挑战。空间信息网格 (Spatial Information Grid,SIG)作为创新性的网络空间信息基础设施和技术体系,为解决这些问题提供了新的契机。本文在给出SIG基本定义和基本特征的基础上,就如何实现基于SIG的矿产资源评价进行了有益的探索和实践,并取得了很好的效果,为矿产资源评价提供了新的方法和思路。
