Toll-like receptor （TLR）-mediated inflammatory response could negatively affect bone metabolism. In this study, we determined how osteogenesis is regulated during inflammatory responses that are downstream of TLR si...Toll-like receptor （TLR）-mediated inflammatory response could negatively affect bone metabolism. In this study, we determined how osteogenesis is regulated during inflammatory responses that are downstream of TLR signaling. Human primary osteoblasts were cultured in collagen gels. Pam3CSK4 （P3C） and Escherichia coli lipopolysaccharide （EcLPS） were used as TLR2 and TLR4 ligand respectively. Porphyromonas gingivalis LPS having TLR2 activity with either TLR4 agonism （Pg1690） or TLR4 antagonism （Pg1449） and mutant E. coli LPS （LPxE/LPxF/WSK） were used. IL-lp, SH2-containing inositol phosphatase-1 （SHIP1） that has regulatory roles in osteogenesis, alkaline phosphatase and mineralization were analyzed. 3α-Aminocholestane （3AC） was used to inhibit SHIP1. Our results suggest that osteoblasts stimulated by P3C, poorly induced IL-1β but strongly upregulated SHIP1 and enhanced osteogenic mediators. On the contrary, EcLPS significantly induced IL-1β and osteogenic mediators were not induced. While Pg1690 downmodulated osteogenic mediators, Pg1449 enhanced osteogenic responses, suggesting that TLR4 signaling annuls osteogenesis even with TLR2 activity. Interestingly, mutant E. coli LPS that induces weak inflammation upregulated osteogenesis, but SHIP1 was not induced. Moreover, inhibiting SHIP1 significantly upregulated TLR2-mediated inflammatory response and downmodulated osteogenesis. In conclusion, these results suggest that induction of weak inflammatory response through TLR2 （with SHIP1 activity） and mutant TLR4 ligands could enhance osteogenesis.展开更多
The properties of 1(1/2)-spectrum are proved and the performances are an- alyzed. By means of the spectrum, the basic frequency component of the harmonic signals can be enhanced. Gaussian color noise and symetrical di...The properties of 1(1/2)-spectrum are proved and the performances are an- alyzed. By means of the spectrum, the basic frequency component of the harmonic signals can be enhanced. Gaussian color noise and symetrical distribution noise can be canceled. And non-quadratic phase coupling harmonic components in harmonic signal can be reduced. The ship radiated-noise is analyzed and its 7 features are extracted by the spectrum. By means of B-P artificial neural network, three type ships are classified according to extracted features. The classification results about the three type ships A, B and C are 90%, 91.3% and 85.7%. respectively.展开更多
We propose a method that automatically generates discrete bicubic G^1 continuous B-spline surfaces that interpolate the curve network of a ship huliform.First,the curves in the network are classified into two types;bo...We propose a method that automatically generates discrete bicubic G^1 continuous B-spline surfaces that interpolate the curve network of a ship huliform.First,the curves in the network are classified into two types;boundary curves and ＂reference curves＂,The boundary curves correspond to a set of rectangular（or triangular）topological type that can be representes with tensot-product （or degenerate）B-spline surface patches.Next,in the interior of the patches,surface fitting points and cross boundary derivatives are estimated from the reference curves by constructing ＂virtual＂isoparametric curves.Finally,a discrete G^1 continuous B-spline surface is gencrated by a surface fitting algorithm.Several smooth ship hullform surfaces generated from curve networks corresponding to actual ship hullforms demonstrate the quality of the method.展开更多
基金supported by Elam M. and Georgina E.Hack Memorial Research Funds,Department of Periodontics,University of Washington,Seattle,WA,USAsupported by WVCTSI funds,West Virginia University,Morgantown,WV,USA
文摘Toll-like receptor （TLR）-mediated inflammatory response could negatively affect bone metabolism. In this study, we determined how osteogenesis is regulated during inflammatory responses that are downstream of TLR signaling. Human primary osteoblasts were cultured in collagen gels. Pam3CSK4 （P3C） and Escherichia coli lipopolysaccharide （EcLPS） were used as TLR2 and TLR4 ligand respectively. Porphyromonas gingivalis LPS having TLR2 activity with either TLR4 agonism （Pg1690） or TLR4 antagonism （Pg1449） and mutant E. coli LPS （LPxE/LPxF/WSK） were used. IL-lp, SH2-containing inositol phosphatase-1 （SHIP1） that has regulatory roles in osteogenesis, alkaline phosphatase and mineralization were analyzed. 3α-Aminocholestane （3AC） was used to inhibit SHIP1. Our results suggest that osteoblasts stimulated by P3C, poorly induced IL-1β but strongly upregulated SHIP1 and enhanced osteogenic mediators. On the contrary, EcLPS significantly induced IL-1β and osteogenic mediators were not induced. While Pg1690 downmodulated osteogenic mediators, Pg1449 enhanced osteogenic responses, suggesting that TLR4 signaling annuls osteogenesis even with TLR2 activity. Interestingly, mutant E. coli LPS that induces weak inflammation upregulated osteogenesis, but SHIP1 was not induced. Moreover, inhibiting SHIP1 significantly upregulated TLR2-mediated inflammatory response and downmodulated osteogenesis. In conclusion, these results suggest that induction of weak inflammatory response through TLR2 （with SHIP1 activity） and mutant TLR4 ligands could enhance osteogenesis.
文摘The properties of 1(1/2)-spectrum are proved and the performances are an- alyzed. By means of the spectrum, the basic frequency component of the harmonic signals can be enhanced. Gaussian color noise and symetrical distribution noise can be canceled. And non-quadratic phase coupling harmonic components in harmonic signal can be reduced. The ship radiated-noise is analyzed and its 7 features are extracted by the spectrum. By means of B-P artificial neural network, three type ships are classified according to extracted features. The classification results about the three type ships A, B and C are 90%, 91.3% and 85.7%. respectively.
文摘We propose a method that automatically generates discrete bicubic G^1 continuous B-spline surfaces that interpolate the curve network of a ship huliform.First,the curves in the network are classified into two types;boundary curves and ＂reference curves＂,The boundary curves correspond to a set of rectangular（or triangular）topological type that can be representes with tensot-product （or degenerate）B-spline surface patches.Next,in the interior of the patches,surface fitting points and cross boundary derivatives are estimated from the reference curves by constructing ＂virtual＂isoparametric curves.Finally,a discrete G^1 continuous B-spline surface is gencrated by a surface fitting algorithm.Several smooth ship hullform surfaces generated from curve networks corresponding to actual ship hullforms demonstrate the quality of the method.
