RNA-directed DNA methylation(Rd DM) is a plant-specific de novo DNA methylation pathway,which has extensive cross-talk with histone modifications. Here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HOMO...RNA-directed DNA methylation(Rd DM) is a plant-specific de novo DNA methylation pathway,which has extensive cross-talk with histone modifications. Here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HOMOLOG 2(SHH2) is an H3 K9 me1 reader. Our structural studies reveal that H3 K9 me1 recognition is achieved by recognition of the methyl group via a classic aromatic cage and hydrogen-bonding and salt-bridge interactions with the free protons of the mono-methyllysine. The di-and tri-methylation states disrupt the polar interactions, decreasing the binding affinity. Our study reveals a monomethyllysine recognition mechanism which potentially links RdDM to H3 K9 me1 in maize.展开更多
探讨了一种基于J2EE技术构建的热处理工艺专家系统的设计思想和实现方法,本系统以目前最为广泛使用的三大框架—Strus2、Spring3、Hibernate作为整个系统的主体框架,并采用以Java为核心技术的专家系统开发工具Jess(Java Expert System S...探讨了一种基于J2EE技术构建的热处理工艺专家系统的设计思想和实现方法,本系统以目前最为广泛使用的三大框架—Strus2、Spring3、Hibernate作为整个系统的主体框架,并采用以Java为核心技术的专家系统开发工具Jess(Java Expert System Shell)开发系统的核心部件。对本系统的最主要的实现部分和关键的代码实现过程进行了解释,并总结得出该热处理专家系统具有智能化、网络化、开放性和个性化的优点。展开更多
基金supported by National Natural Science Foundation of China(31770782)the Ministry of Science and Technology of China(2016YFA0503200)+1 种基金the Shenzhen Science and Technology Program(JCYJ20200109110403829 and KQTD20190929173906742)Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes(2019KSYS006)to J.D.
文摘RNA-directed DNA methylation(Rd DM) is a plant-specific de novo DNA methylation pathway,which has extensive cross-talk with histone modifications. Here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HOMOLOG 2(SHH2) is an H3 K9 me1 reader. Our structural studies reveal that H3 K9 me1 recognition is achieved by recognition of the methyl group via a classic aromatic cage and hydrogen-bonding and salt-bridge interactions with the free protons of the mono-methyllysine. The di-and tri-methylation states disrupt the polar interactions, decreasing the binding affinity. Our study reveals a monomethyllysine recognition mechanism which potentially links RdDM to H3 K9 me1 in maize.
文摘探讨了一种基于J2EE技术构建的热处理工艺专家系统的设计思想和实现方法,本系统以目前最为广泛使用的三大框架—Strus2、Spring3、Hibernate作为整个系统的主体框架,并采用以Java为核心技术的专家系统开发工具Jess(Java Expert System Shell)开发系统的核心部件。对本系统的最主要的实现部分和关键的代码实现过程进行了解释,并总结得出该热处理专家系统具有智能化、网络化、开放性和个性化的优点。
