To find optimal conditions for expressing the soluble form of sFv-2F3 and to study the purification and property of its derivative Se-sFv-2F3,the preferred expression conditions were investigated by means of orthogona...To find optimal conditions for expressing the soluble form of sFv-2F3 and to study the purification and property of its derivative Se-sFv-2F3,the preferred expression conditions were investigated by means of orthogonal design. These culture conditions included incubation temperature,inducer concentration, induction time and cell concentration. The evaluation of expression was accomplished by the analysis of whole cell lysates and the yield of soluble sFv-2F3 was calculated according to the analysis of Profinder(FTI-500,Pharmacia). The purification procedure was carried out via a two-step purification procedure consisting of ion-exchange chromatography,followed by immobilized metal affinity chromatography(IMAC). The antioxidant efficacy of Se-sFv-2F3 was demonstrated by the determination of the content of the main product of lipid peroxidation,MDA,the viability of cells and the activity of LDH. We obtained the preferred culture conditions to grow the engineered bacteria and the procedure for preparing soluble sFv-2F3 and confirmed the antioxidant efficacy of Se-sFv-2F3.展开更多
For the sustainable development of China auto industry, PES’96 on Electric Vehicles and Substitutional Fuel Vehicles as held in Beijing from Dec. 6 to Dec. 14 of 1996. This PSE ’96, which w.as another important acti...For the sustainable development of China auto industry, PES’96 on Electric Vehicles and Substitutional Fuel Vehicles as held in Beijing from Dec. 6 to Dec. 14 of 1996. This PSE ’96, which w.as another important activity in China auto sector this year, was paid a great attention from the auto circles home and abroad. Present at the opening ceremony were Bao展开更多
Ⅰ. Sustainable development strategy of China auto industry China auto industry is in the process of development. China’s auto products only take a comparatively small share in the world auto market. However, we have...Ⅰ. Sustainable development strategy of China auto industry China auto industry is in the process of development. China’s auto products only take a comparatively small share in the world auto market. However, we have attached great importance to the sustainable development strategy of China auto industry which, we think, is a展开更多
Ⅰ. Strategic Concept for the development of EV and SFV The development of EV and SFV is one of the objective requirements for the auto industry in the 21st century by sustainable development strategy. It is also a pr...Ⅰ. Strategic Concept for the development of EV and SFV The development of EV and SFV is one of the objective requirements for the auto industry in the 21st century by sustainable development strategy. It is also a practicable way of developing China auto industry in line with the environmental protection policy and the energy展开更多
文摘To find optimal conditions for expressing the soluble form of sFv-2F3 and to study the purification and property of its derivative Se-sFv-2F3,the preferred expression conditions were investigated by means of orthogonal design. These culture conditions included incubation temperature,inducer concentration, induction time and cell concentration. The evaluation of expression was accomplished by the analysis of whole cell lysates and the yield of soluble sFv-2F3 was calculated according to the analysis of Profinder(FTI-500,Pharmacia). The purification procedure was carried out via a two-step purification procedure consisting of ion-exchange chromatography,followed by immobilized metal affinity chromatography(IMAC). The antioxidant efficacy of Se-sFv-2F3 was demonstrated by the determination of the content of the main product of lipid peroxidation,MDA,the viability of cells and the activity of LDH. We obtained the preferred culture conditions to grow the engineered bacteria and the procedure for preparing soluble sFv-2F3 and confirmed the antioxidant efficacy of Se-sFv-2F3.
文摘For the sustainable development of China auto industry, PES’96 on Electric Vehicles and Substitutional Fuel Vehicles as held in Beijing from Dec. 6 to Dec. 14 of 1996. This PSE ’96, which w.as another important activity in China auto sector this year, was paid a great attention from the auto circles home and abroad. Present at the opening ceremony were Bao
文摘Ⅰ. Sustainable development strategy of China auto industry China auto industry is in the process of development. China’s auto products only take a comparatively small share in the world auto market. However, we have attached great importance to the sustainable development strategy of China auto industry which, we think, is a
文摘Ⅰ. Strategic Concept for the development of EV and SFV The development of EV and SFV is one of the objective requirements for the auto industry in the 21st century by sustainable development strategy. It is also a practicable way of developing China auto industry in line with the environmental protection policy and the energy
