Severe fever with thrombocytopenia syndrome virus(SFTSV),the causative agent of a febrile human disease,was first identified from central and eastern provinces in China,and later in Japan and South Korea.Hubei Provinc...Severe fever with thrombocytopenia syndrome virus(SFTSV),the causative agent of a febrile human disease,was first identified from central and eastern provinces in China,and later in Japan and South Korea.Hubei Province is one of the major SFTS epidemic areas in the central part of China.This study reported the isolation of 11 new SFTSV strains from patients in Hubei Province collected in 2017.Extensive phylogenetic analyses were conducted based on the complete coding sequences of SFTSV segments including the new strains.It was suggested that five different SFTSV genotypes were circulating in Hubei,and 15 reassortment patterns and migration pathways correlated with each genotype were identified,which was more than previously recognized.Hubei Province was more involved in the evolutionary events of SFTSV than that previously thought in which the evolutionary events of SFTSV were reported to be independent from those in other epidemic regions.Further divergence of SFTSV strains was suggested by pairwise comparison of SFTSV sequences from each genotype and sequence identity normalized to representative strain in genotype C1.Subsequently,amino acid variations specific for genotype(s),strain(s),or cluster(s)were inspected,which may be related to differential biological activity of SFTSV strains/genotypes.In conclusion,we analyzed the current status of SFTSV phylogeny in Hubei Province and discussed the possible events correlated to SFTSV evolution.It provided an in-depth insight into SFTSV evolution,raising concerns for the use of proper SFTSV strains in future studies.展开更多
严重发热伴血小板减少综合征(SFTS,severe fever and thrombocytopenia syndrome)是由一种新型布尼亚病毒SFTS病毒(severe fever and thrombocytopenia syndrome Virus,SFTSV)引起的传染病。由于SFTS的病死率可高达30%,因此需要对SFTS...严重发热伴血小板减少综合征(SFTS,severe fever and thrombocytopenia syndrome)是由一种新型布尼亚病毒SFTS病毒(severe fever and thrombocytopenia syndrome Virus,SFTSV)引起的传染病。由于SFTS的病死率可高达30%,因此需要对SFTS进行有效的针对性治疗。近年来针对SFTS抗病毒药物的研究表明,利巴韦林和法维吡拉韦的疗效较好。目前在体外试验和动物模型中,法维吡拉韦对SFTS的疗效明显高于利巴韦林。其它的药物,包括六氯酚、钙通道阻滞剂、2′-氟-2′-脱氧胞苷、咖啡酸、阿莫地喹和干扰素也有效的对SFTSV的增殖起到抑制作用。研究显示钙通道阻滞剂硝苯地平除了在体内、外的疗效外,还能降低临床病死率。本文对以上几种抗SFTSV药物研究进行了总结,并讨论抗SFTSV药物开发的最新进展。展开更多
Severe fever with thrombocytopenia syndrome virus(SFTSV)is an emerging bunyavirus that causes severe fever with thrombocytopenia syndrome(SFTS)[1]and is showing a trend toward global transmission[2].Therefore,a compre...Severe fever with thrombocytopenia syndrome virus(SFTSV)is an emerging bunyavirus that causes severe fever with thrombocytopenia syndrome(SFTS)[1]and is showing a trend toward global transmission[2].Therefore,a comprehensive understanding of the pathogenic mechanism of SFTSV remains crucial.展开更多
Given the overlapping endemic regions and clinical similarities between severe fever with thrombocytopenia syndrome(SFTS)and hemorrhagic fever with renal syndrome(HFRS),we developed a dual real-time fluorescence-based...Given the overlapping endemic regions and clinical similarities between severe fever with thrombocytopenia syndrome(SFTS)and hemorrhagic fever with renal syndrome(HFRS),we developed a dual real-time fluorescence-based reverse transcription quantitative polymerase chain reaction(RT-qPCR)method.Recombinant plasmids and synthetic ribonucleic acid(RNA)were constructed to evaluate the specificity,sensitivity and reproducibility of the assay.Additionally,we assessed the specificity of the assay using samples from three distinct groups:individuals with confirmed severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection(n=10),influenza A-positive individuals(n=10),and healthy controls.Receiver operating characteristic(ROC)curves were used to assess diagnostic accuracy,while the Kappa coefficient and linear regression analysis were employed to evaluate clinical applicability.Our method exhibited specificity for both SFTSV and Hantaan virus detection,with detection limits of 333 and 1,022 copies/mL using plasmids,and 1,247 and 898 copies/mL using synthetic RNA,respectively.We evaluated 100 clinical samples from each of SFTS and HFRS.The Kappa coefficients for both diseases were 0.96.The areas under the ROC curves were 0.991(P<0.001)and 0.989(P<0.001),respectively.The linear regression equations were as follows:log(y)=0.19+0.99 log(x)(R^(2)=0.95)for SFTS virus,and log(y)=0.01+0.65 log(x)(R^(2)=0.92)for Hantaan virus.We established an in-house RT-qPCR method for the rapid quantification of both pathogens,making it an ideal tool for early clinical differentiation.展开更多
Severe fever with thrombocytopenia syndrome(SFTS) is an emerging hemorrhagic fever in rural areas of China and is caused by a new bunyavirus,SFTSV,named after the disease.The transmission vectors and animal hosts of S...Severe fever with thrombocytopenia syndrome(SFTS) is an emerging hemorrhagic fever in rural areas of China and is caused by a new bunyavirus,SFTSV,named after the disease.The transmission vectors and animal hosts of SFTSV are unclear.Ticks are the most likely transmission vectors and domestic animals,including goats,dogs,and cattle,are potential amplifying hosts of SFTSV.The clinical symptoms of SFTS are nonspecific,but major symptoms include fever,gastrointestinal symptoms,myalgia,dizziness,joint pain,chills,and regional lymphadenopathy.The most common abnormalities in laboratory test results are thrombocytopenia(95%),leukocytopenia(86%),and elevated levels of serum alanine aminotransferase,aspartate aminotransferase,creatine kinase,and lactate dehydrogenase.The fatality rate for SFTS is 12% on average,and the annual incidence of the disease is approximately five per 100000 of the rural population.展开更多
Tales of tracing the origins of human immunodeficiency virus(HIV),human coronavirus HKU1(HCoV-HKU1),severe fever with thrombocytopenia syndrome virus(SFTSV),and Middle East respiratory syndrome coronavirus(MERS-CoV)ca...Tales of tracing the origins of human immunodeficiency virus(HIV),human coronavirus HKU1(HCoV-HKU1),severe fever with thrombocytopenia syndrome virus(SFTSV),and Middle East respiratory syndrome coronavirus(MERS-CoV)can enlighten the scientific search for the origins of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Here,we detail key research studies on the origins of these four viruses.展开更多
Severe fever with thrombocytopenia syndrome(SFTS)is a novel emerging acute infectious disease caused by severe fever with thrombocytopenia syndrome virus(SFTSV),characterized by high fever and thrombocytopenia.It has ...Severe fever with thrombocytopenia syndrome(SFTS)is a novel emerging acute infectious disease caused by severe fever with thrombocytopenia syndrome virus(SFTSV),characterized by high fever and thrombocytopenia.It has been proved that traditional Chinese medicine(TCM)has displayed definite therapeutic effects on viral hemorrhagic fever,indicating its potential to treat SFTS.In this study,SFTS-relative key targets were predicted via gene ontology(GO)analysis and kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis.Molecular docking was then used to select stable binders.Molecules matched TCMs were identified,and a new prescription,Qingqi Guxue decoction(QQGX),was formulated to clear heat and nourish blood,with a resulting drug composition network.We explored the optimal drug proportion for QQGX.Through an in-depth study of molecular mechanisms,we found that QQGX induces S phase arrest by promoting the degradation of cyclin A2(CCNA2)and cyclin-dependent kinase 2(CDK2),thereby inhibiting SFTSV replication.Finally,we verified the effectiveness and safety of QQGX based on the mouse liver bile duct organoid model infected with SFTSV.In summary,our study prepared a TCM decoction using the method of network pharmacology.This decoction has a significant inhibitory effect on the replication of SFTSV and provides a new treatment strategy for hemorrhagic fever with TCM.展开更多
发热伴血小板减少综合征(severe fever with thrombocytopenia syndrome,SFTS)是由一种新型布尼亚病毒(severe fever with thrombocytopenia syndrome bunyavirus,SFTSV)引发的急性传染病。该病于2009年在中国首次发现,韩国、日本等东...发热伴血小板减少综合征(severe fever with thrombocytopenia syndrome,SFTS)是由一种新型布尼亚病毒(severe fever with thrombocytopenia syndrome bunyavirus,SFTSV)引发的急性传染病。该病于2009年在中国首次发现,韩国、日本等东亚国家和地区也有病例报道[1]。其主要临床特征包括高热、血小板减少及多器官功能障碍,重症病例可致死,病死率为10%~15%[2]。SFTSV属于布尼亚病毒科,是一种新型蜱传病毒,主要通过蜱虫叮咬和血液途径传播[3]。近年来,随着研究的深入,SFTS在流行病学、致病机制、诊断和治疗等方面取得重要进展。本文综述其病原学、流行病学、临床表现、诊断和治疗的最新研究,以期为该病的综合防治提供理论支持与实践参考。展开更多
Haemaphysalis longicornis serves as the primary tick vector for severe fever with thrombocytopenia syndrome virus(SFTSV),the etiological agent responsible for severe fever with thrombocytopenia syndrome(SFTS).Understa...Haemaphysalis longicornis serves as the primary tick vector for severe fever with thrombocytopenia syndrome virus(SFTSV),the etiological agent responsible for severe fever with thrombocytopenia syndrome(SFTS).Understanding alterations in tick salivary gland microbiota during SFTSV transmission to vertebrate hosts is essential for developing novel control strategies.However,microbial shifts in tick salivary glands during pathogen transmission to hosts have not been reported for any tick-borne pathogens.In this study,SFTSV transmission from H.longicornis to vertebrate hosts was confirmed using a tickrabbit transmission model.Salivary gland microbiota profiling via 16S rRNA gene sequencing identified significant changes in bacterial composition associated with viral transmission.The relative abundance of three genera(Serratia,Bifidobacterium,and Akkermansia)increased,whereas five genera(Flavobacterium,Staphylococcus,Enhydrobacter,Massilia,and Stenotrophomonas)decreased.Correlation network analysis revealed a negative association between Akkermansia and Flavobacterium.These findings demonstrated that SFTSV transmission alters the salivary gland microbiota of H.longicornis,providing insights for future functional studies and the development of targeted strategies for SFTS control.展开更多
Severe fever with thrombocytopenia syndrome virus(SFTSV),an emerging tick-borne pathogen,has caused a rising number of human cases in the urban-rural fringe of Beijing since 2021.This study explores the seasonal dynam...Severe fever with thrombocytopenia syndrome virus(SFTSV),an emerging tick-borne pathogen,has caused a rising number of human cases in the urban-rural fringe of Beijing since 2021.This study explores the seasonal dynamics of hedgehog-associated ticks and SFTSV transmission in urban parks of Beijing.Surveys across six parks revealed distinct activity patterns:adult Haemaphysalis longicornis peaked in summer,while nymphs dominated spring and autumn.All collected H.longicornis belonged to parthenogenetic populations.A near-complete SFTSV genome(C4 strain)was identified in a tick collected from Taoranting Park,suggesting multiple viral introductions into Beijing.Serological analysis showed that>50%of hedgehogs carried SFTSV-neutralizing antibodies in spring;yet seropositivity declined markedly in summer and autumn,indicating recurrent infections and implicating hedgehogs as potential reservoirs.These findings reveal an urban SFTSV transmission cycle maintained by hedgehogs and parthenogenetic H.longicornis,emphasizing the urgency of enhanced surveillance and public health interventions to curb urban zoonotic risks.展开更多
基金supported by the National Key R&D Programme of China(2018ZX10734404-010)the Nature Science Foundation of Hubei Province(2019CFB790 and 2018CFB471)+2 种基金the Innovation Team Project of Hubei Provincial Health Commission(WJ2019C003)the Open Research Fund of State Key Laboratory of Virology(2018IOV004)the Special Project of Technical Conditions(2060503)。
文摘Severe fever with thrombocytopenia syndrome virus(SFTSV),the causative agent of a febrile human disease,was first identified from central and eastern provinces in China,and later in Japan and South Korea.Hubei Province is one of the major SFTS epidemic areas in the central part of China.This study reported the isolation of 11 new SFTSV strains from patients in Hubei Province collected in 2017.Extensive phylogenetic analyses were conducted based on the complete coding sequences of SFTSV segments including the new strains.It was suggested that five different SFTSV genotypes were circulating in Hubei,and 15 reassortment patterns and migration pathways correlated with each genotype were identified,which was more than previously recognized.Hubei Province was more involved in the evolutionary events of SFTSV than that previously thought in which the evolutionary events of SFTSV were reported to be independent from those in other epidemic regions.Further divergence of SFTSV strains was suggested by pairwise comparison of SFTSV sequences from each genotype and sequence identity normalized to representative strain in genotype C1.Subsequently,amino acid variations specific for genotype(s),strain(s),or cluster(s)were inspected,which may be related to differential biological activity of SFTSV strains/genotypes.In conclusion,we analyzed the current status of SFTSV phylogeny in Hubei Province and discussed the possible events correlated to SFTSV evolution.It provided an in-depth insight into SFTSV evolution,raising concerns for the use of proper SFTSV strains in future studies.
文摘严重发热伴血小板减少综合征(SFTS,severe fever and thrombocytopenia syndrome)是由一种新型布尼亚病毒SFTS病毒(severe fever and thrombocytopenia syndrome Virus,SFTSV)引起的传染病。由于SFTS的病死率可高达30%,因此需要对SFTS进行有效的针对性治疗。近年来针对SFTS抗病毒药物的研究表明,利巴韦林和法维吡拉韦的疗效较好。目前在体外试验和动物模型中,法维吡拉韦对SFTS的疗效明显高于利巴韦林。其它的药物,包括六氯酚、钙通道阻滞剂、2′-氟-2′-脱氧胞苷、咖啡酸、阿莫地喹和干扰素也有效的对SFTSV的增殖起到抑制作用。研究显示钙通道阻滞剂硝苯地平除了在体内、外的疗效外,还能降低临床病死率。本文对以上几种抗SFTSV药物研究进行了总结,并讨论抗SFTSV药物开发的最新进展。
基金supported by the National Natural Science Foundation of China(32470146 and 32170144).
文摘Severe fever with thrombocytopenia syndrome virus(SFTSV)is an emerging bunyavirus that causes severe fever with thrombocytopenia syndrome(SFTS)[1]and is showing a trend toward global transmission[2].Therefore,a comprehensive understanding of the pathogenic mechanism of SFTSV remains crucial.
基金supported by the National Key R&D Program of China(2022YFF1203201)National Natural Science Foundation of China(82072295)Beijing Research Center for Respiratory Infectious Diseases Project(BJRID2024-009).
文摘Given the overlapping endemic regions and clinical similarities between severe fever with thrombocytopenia syndrome(SFTS)and hemorrhagic fever with renal syndrome(HFRS),we developed a dual real-time fluorescence-based reverse transcription quantitative polymerase chain reaction(RT-qPCR)method.Recombinant plasmids and synthetic ribonucleic acid(RNA)were constructed to evaluate the specificity,sensitivity and reproducibility of the assay.Additionally,we assessed the specificity of the assay using samples from three distinct groups:individuals with confirmed severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection(n=10),influenza A-positive individuals(n=10),and healthy controls.Receiver operating characteristic(ROC)curves were used to assess diagnostic accuracy,while the Kappa coefficient and linear regression analysis were employed to evaluate clinical applicability.Our method exhibited specificity for both SFTSV and Hantaan virus detection,with detection limits of 333 and 1,022 copies/mL using plasmids,and 1,247 and 898 copies/mL using synthetic RNA,respectively.We evaluated 100 clinical samples from each of SFTS and HFRS.The Kappa coefficients for both diseases were 0.96.The areas under the ROC curves were 0.991(P<0.001)and 0.989(P<0.001),respectively.The linear regression equations were as follows:log(y)=0.19+0.99 log(x)(R^(2)=0.95)for SFTS virus,and log(y)=0.01+0.65 log(x)(R^(2)=0.92)for Hantaan virus.We established an in-house RT-qPCR method for the rapid quantification of both pathogens,making it an ideal tool for early clinical differentiation.
文摘Severe fever with thrombocytopenia syndrome(SFTS) is an emerging hemorrhagic fever in rural areas of China and is caused by a new bunyavirus,SFTSV,named after the disease.The transmission vectors and animal hosts of SFTSV are unclear.Ticks are the most likely transmission vectors and domestic animals,including goats,dogs,and cattle,are potential amplifying hosts of SFTSV.The clinical symptoms of SFTS are nonspecific,but major symptoms include fever,gastrointestinal symptoms,myalgia,dizziness,joint pain,chills,and regional lymphadenopathy.The most common abnormalities in laboratory test results are thrombocytopenia(95%),leukocytopenia(86%),and elevated levels of serum alanine aminotransferase,aspartate aminotransferase,creatine kinase,and lactate dehydrogenase.The fatality rate for SFTS is 12% on average,and the annual incidence of the disease is approximately five per 100000 of the rural population.
文摘Tales of tracing the origins of human immunodeficiency virus(HIV),human coronavirus HKU1(HCoV-HKU1),severe fever with thrombocytopenia syndrome virus(SFTSV),and Middle East respiratory syndrome coronavirus(MERS-CoV)can enlighten the scientific search for the origins of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Here,we detail key research studies on the origins of these four viruses.
基金supported by the National Natural Science Foundation of China(32170144 and 32470146).
文摘Severe fever with thrombocytopenia syndrome(SFTS)is a novel emerging acute infectious disease caused by severe fever with thrombocytopenia syndrome virus(SFTSV),characterized by high fever and thrombocytopenia.It has been proved that traditional Chinese medicine(TCM)has displayed definite therapeutic effects on viral hemorrhagic fever,indicating its potential to treat SFTS.In this study,SFTS-relative key targets were predicted via gene ontology(GO)analysis and kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis.Molecular docking was then used to select stable binders.Molecules matched TCMs were identified,and a new prescription,Qingqi Guxue decoction(QQGX),was formulated to clear heat and nourish blood,with a resulting drug composition network.We explored the optimal drug proportion for QQGX.Through an in-depth study of molecular mechanisms,we found that QQGX induces S phase arrest by promoting the degradation of cyclin A2(CCNA2)and cyclin-dependent kinase 2(CDK2),thereby inhibiting SFTSV replication.Finally,we verified the effectiveness and safety of QQGX based on the mouse liver bile duct organoid model infected with SFTSV.In summary,our study prepared a TCM decoction using the method of network pharmacology.This decoction has a significant inhibitory effect on the replication of SFTSV and provides a new treatment strategy for hemorrhagic fever with TCM.
文摘发热伴血小板减少综合征(severe fever with thrombocytopenia syndrome,SFTS)是由一种新型布尼亚病毒(severe fever with thrombocytopenia syndrome bunyavirus,SFTSV)引发的急性传染病。该病于2009年在中国首次发现,韩国、日本等东亚国家和地区也有病例报道[1]。其主要临床特征包括高热、血小板减少及多器官功能障碍,重症病例可致死,病死率为10%~15%[2]。SFTSV属于布尼亚病毒科,是一种新型蜱传病毒,主要通过蜱虫叮咬和血液途径传播[3]。近年来,随着研究的深入,SFTS在流行病学、致病机制、诊断和治疗等方面取得重要进展。本文综述其病原学、流行病学、临床表现、诊断和治疗的最新研究,以期为该病的综合防治提供理论支持与实践参考。
基金supported by the National Natural Science Foundation of China(82102433,32160124,32370522,32090014)Hainan Provincial Natural Science Foundation of China(822RC711,821QN254)。
文摘Haemaphysalis longicornis serves as the primary tick vector for severe fever with thrombocytopenia syndrome virus(SFTSV),the etiological agent responsible for severe fever with thrombocytopenia syndrome(SFTS).Understanding alterations in tick salivary gland microbiota during SFTSV transmission to vertebrate hosts is essential for developing novel control strategies.However,microbial shifts in tick salivary glands during pathogen transmission to hosts have not been reported for any tick-borne pathogens.In this study,SFTSV transmission from H.longicornis to vertebrate hosts was confirmed using a tickrabbit transmission model.Salivary gland microbiota profiling via 16S rRNA gene sequencing identified significant changes in bacterial composition associated with viral transmission.The relative abundance of three genera(Serratia,Bifidobacterium,and Akkermansia)increased,whereas five genera(Flavobacterium,Staphylococcus,Enhydrobacter,Massilia,and Stenotrophomonas)decreased.Correlation network analysis revealed a negative association between Akkermansia and Flavobacterium.These findings demonstrated that SFTSV transmission alters the salivary gland microbiota of H.longicornis,providing insights for future functional studies and the development of targeted strategies for SFTS control.
基金supported by the National Key Plan for Scientific Research and Development of China(2024YFC2607500)the Initiative Scientific Research Program,Institute of Zoology,Chinese Academy of Sciences(2023IOZ0205)the National Natural Science Foundation of China(U24A20363).
文摘Severe fever with thrombocytopenia syndrome virus(SFTSV),an emerging tick-borne pathogen,has caused a rising number of human cases in the urban-rural fringe of Beijing since 2021.This study explores the seasonal dynamics of hedgehog-associated ticks and SFTSV transmission in urban parks of Beijing.Surveys across six parks revealed distinct activity patterns:adult Haemaphysalis longicornis peaked in summer,while nymphs dominated spring and autumn.All collected H.longicornis belonged to parthenogenetic populations.A near-complete SFTSV genome(C4 strain)was identified in a tick collected from Taoranting Park,suggesting multiple viral introductions into Beijing.Serological analysis showed that>50%of hedgehogs carried SFTSV-neutralizing antibodies in spring;yet seropositivity declined markedly in summer and autumn,indicating recurrent infections and implicating hedgehogs as potential reservoirs.These findings reveal an urban SFTSV transmission cycle maintained by hedgehogs and parthenogenetic H.longicornis,emphasizing the urgency of enhanced surveillance and public health interventions to curb urban zoonotic risks.
