严重发热伴血小板减少综合征(SFTS,severe fever and thrombocytopenia syndrome)是由一种新型布尼亚病毒SFTS病毒(severe fever and thrombocytopenia syndrome Virus,SFTSV)引起的传染病。由于SFTS的病死率可高达30%,因此需要对SFTS...严重发热伴血小板减少综合征(SFTS,severe fever and thrombocytopenia syndrome)是由一种新型布尼亚病毒SFTS病毒(severe fever and thrombocytopenia syndrome Virus,SFTSV)引起的传染病。由于SFTS的病死率可高达30%,因此需要对SFTS进行有效的针对性治疗。近年来针对SFTS抗病毒药物的研究表明,利巴韦林和法维吡拉韦的疗效较好。目前在体外试验和动物模型中,法维吡拉韦对SFTS的疗效明显高于利巴韦林。其它的药物,包括六氯酚、钙通道阻滞剂、2′-氟-2′-脱氧胞苷、咖啡酸、阿莫地喹和干扰素也有效的对SFTSV的增殖起到抑制作用。研究显示钙通道阻滞剂硝苯地平除了在体内、外的疗效外,还能降低临床病死率。本文对以上几种抗SFTSV药物研究进行了总结,并讨论抗SFTSV药物开发的最新进展。展开更多
A single-mode-few-mode-thin-core-single-mode(SFTS) structure based optical fiber sensor is fabricated and experimentally studied. The sensing principle relies on the inter-modal interference. Since the core diameter o...A single-mode-few-mode-thin-core-single-mode(SFTS) structure based optical fiber sensor is fabricated and experimentally studied. The sensing principle relies on the inter-modal interference. Since the core diameter of few-mode fiber(FMF) is larger than that of single-mode fiber(SMF), the FMF helps to allow more light to enter the cladding of thin-core fiber(TCF), which helps TCF to excite cladding modes. The interference between core and cladding modes in TCF occurs at the joint of lead-out SMF and TCF. Experimental results demonstrate a refractive index(RI) sensitivity of-103.34 nm/RIU and a temperature sensitivity of 0.05 nm/℃. The proposed sensor not only can measure temperature, but also can measure RI. In addition, the proposed sensor is simple for without complicated fabrication process.展开更多
Given the overlapping endemic regions and clinical similarities between severe fever with thrombocytopenia syndrome(SFTS)and hemorrhagic fever with renal syndrome(HFRS),we developed a dual real-time fluorescence-based...Given the overlapping endemic regions and clinical similarities between severe fever with thrombocytopenia syndrome(SFTS)and hemorrhagic fever with renal syndrome(HFRS),we developed a dual real-time fluorescence-based reverse transcription quantitative polymerase chain reaction(RT-qPCR)method.Recombinant plasmids and synthetic ribonucleic acid(RNA)were constructed to evaluate the specificity,sensitivity and reproducibility of the assay.Additionally,we assessed the specificity of the assay using samples from three distinct groups:individuals with confirmed severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection(n=10),influenza A-positive individuals(n=10),and healthy controls.Receiver operating characteristic(ROC)curves were used to assess diagnostic accuracy,while the Kappa coefficient and linear regression analysis were employed to evaluate clinical applicability.Our method exhibited specificity for both SFTSV and Hantaan virus detection,with detection limits of 333 and 1,022 copies/mL using plasmids,and 1,247 and 898 copies/mL using synthetic RNA,respectively.We evaluated 100 clinical samples from each of SFTS and HFRS.The Kappa coefficients for both diseases were 0.96.The areas under the ROC curves were 0.991(P<0.001)and 0.989(P<0.001),respectively.The linear regression equations were as follows:log(y)=0.19+0.99 log(x)(R^(2)=0.95)for SFTS virus,and log(y)=0.01+0.65 log(x)(R^(2)=0.92)for Hantaan virus.We established an in-house RT-qPCR method for the rapid quantification of both pathogens,making it an ideal tool for early clinical differentiation.展开更多
SFTS virus(SFTSV)is a novel bunyavirus,which was discovered as the etiological agent of severe fever with thrombocytopenia syndrome(SFTS)in China in 2009,and was now prevalent in at least 25 provinces in China.SFTS wa...SFTS virus(SFTSV)is a novel bunyavirus,which was discovered as the etiological agent of severe fever with thrombocytopenia syndrome(SFTS)in China in 2009,and was now prevalent in at least 25 provinces in China.SFTS was subsequently identified in South Korea and Japan in 2012.To explore themolecular evolution and genetic characteristics of this newly identified pathogen,we reported 72 whole genome sequences of SFTSV,and built a dataset of SFTSV genome sequences containing 292 L-segment,302 M-segment and 502 S-segment.We clearly divided SFTSV into six genotypes,Genotype A-F.It was found that genotype F was the dominant epidemic genotype of Japan,South Korea,and Zhejiang province of China.The coalescent analysis supported that SFTSV originated in the early 18th century from Zhejiang province,and Genotype F was the most primitive one.Henan,Hubei,and Anhui provinces which are located in Dabie Mountain area weremainly epidemic of Genotype A,which emerged relatively late but distributed widely.A total of 37 recombination events were identified,making SFTSV with a high recombination frequency(L segment 5.1%,Msegment 3.6%,S segment 0.8%)among negative-strand segmented RNA viruses.It was identified that 19 reassortant strains belonged to 12 reassortment forms of SFTSV genome containing 6 newly identified forms.The reassortment virus and recombination in tick were both found for the first time.We also found many of genotype-specific mutation sites,7 of which could be considered as potential molecular marker for genotype classification.This study promoted a more comprehensive understanding of the phylogeny and origin,and the genetic diversity of SFTSV,and it could help the studies of other newly discovered tick-borne bunyavirus as reference data and research ideas.展开更多
Severe fever with thrombocytopenia syndrome(SFTS),caused by a novel identified bunyavirus SFTS virus(SFTSV),was an emerging viral infectious disease that was firstly reported in China.There are no licensed vaccines an...Severe fever with thrombocytopenia syndrome(SFTS),caused by a novel identified bunyavirus SFTS virus(SFTSV),was an emerging viral infectious disease that was firstly reported in China.There are no licensed vaccines and therapeutics against SFTSV currently.B‐Propiolactone(BPL)inactivated whole virions of SFTSV strain AH12 were prepared as experimental vaccine in different antigen dose with or without Al(OH)3 adjuvant.The experimental SFTS vaccine was a satisfying immunogen,which could efficiently trigger the development of high levels of SFTSV NP‐specific IgG antibodies and neutralizing antibodies against SFTSV Strain HB29 in BALB/c and C57/BL6 mice,and could induce SFTS virus‐specific cellular immune responses to a certain extent.A single dose of vaccine was immunogenically insufficient in BALB/c mice;the second and third dose resulted in significant boost in antibody response.The use of Al(OH)3 adjuvant resulted in higher antibody titers.The mediate‐dose of vaccine could induce as high and equivalent level of antibody titer as that of high‐dose.The experimental SFTS vaccine in mediate‐and high antigen dose with adjuvant resulted in solid protection of C57/BL6 mice against wild‐type SFTSV challenge with markedly accelerated virus clearance from blood and spleen compared with controls.The experimental SFTS vaccine prepared in this study could efficiently elicit virus specific humoral and cellular immune responses in both BALB/c and C57/BL6 mice,and could protect C57/BL6 mice against SFTS virus challenge.These results supplied evidence that inactivated vaccine was a promising vaccine candidate for the prevention of SFTSV infection.展开更多
Code acquisition is the kernel operation for signal synchronization in the spread-spectrum receiver.To reduce the computational complexity and latency of code acquisition,this paper proposes an efficient scheme employ...Code acquisition is the kernel operation for signal synchronization in the spread-spectrum receiver.To reduce the computational complexity and latency of code acquisition,this paper proposes an efficient scheme employing sparse Fourier transform(SFT)and the relevant hardware architecture for field programmable gate array(FPGA)and application-specific integrated circuit(ASIC)implementation.Efforts are made at both the algorithmic level and the implementation level to enable merged searching of code phase and Doppler frequency without incurring massive hardware expenditure.Compared with the existing code acquisition approaches,it is shown from theoretical analysis and experimental results that the proposed design can shorten processing latency and reduce hardware complexity without degrading the acquisition probability.展开更多
目的分析2012-2014年临海市发热伴血小板减少综合征(severe fever with thrombocytopenia syndrome,SFTS)流行特点及病原学监测结果,为临海市SFTS防控策略的制定提供依据,同时为其他低度流行区的SFTS防控提供参考。方法应用描述流行病...目的分析2012-2014年临海市发热伴血小板减少综合征(severe fever with thrombocytopenia syndrome,SFTS)流行特点及病原学监测结果,为临海市SFTS防控策略的制定提供依据,同时为其他低度流行区的SFTS防控提供参考。方法应用描述流行病学方法分析临海市2012-2014年SFTS病例特征及病原学监测结果。结果2012年临海市首次报告SFTS,到2014年出现一次集中于局部地区的流行,发病率较2012年显著上升(P<0.05);病例发生主要集中在4-5月份,只有1个发病高峰;患者中男性4例,男性发病率为0.649/10万,女性5例,女性发病率为0.881/10万,男女SFTS发病率差异无统计学意义(P>0.05);患者年龄主要集中在50-70岁之间,占77.8%;职业以农民为主,占88.9%;报告单位以本地综合性医疗机构为主,占77.8%。就诊不及时及诊断延误是造成SFTS病死率较高的主要原因。病例分布及病原学监测表明临海市的SFTS疫情属于局部暴发流行,66.7%的患者均来自白水洋镇黄坦乡或有此地野外暴露史,在患者居住地周围采集到的蜱虫标本检测3组布尼亚病毒核酸阳性,说明存在自然疫源地。结论临海市SFTS疫情具有明显的地域性和季节性,2014年发病率有升高趋势,并且存在自然疫源地,高龄农民为高危人群,SFTSV仍为主要病原,就诊不及时及诊断的延误是造成SFTS病死率高的主要原因。展开更多
Severe fever with thrombocytopenia syndrome （SFTS） is an emerging infectious disease caused by SFTS virus （SFTSV）. SFTSV is associated with a high mortality rate and has been reported in China, South Korea and Jap...Severe fever with thrombocytopenia syndrome （SFTS） is an emerging infectious disease caused by SFTS virus （SFTSV）. SFTSV is associated with a high mortality rate and has been reported in China, South Korea and Japan. SFTSV undergoes rapid changes owing to evolution, gene mutations, and reassortment between different strains of SFTSV. In this review, we summarize the recent cases and general properties of SFTS, focusing on the epidemiology, genetic diversity, clinical features, and diagnostics of SFTSV in China. From 2010 to October 2016, SFTS cases were reported in 23 provinces of China, with increased numbers yearly. Infection and death cases are mainly found in central China, where the Haemaphysalis Iongicornis ticks are spread. The national average mortality rate of SFTS infection was 5.3%, with higher risk to elder people. The main epidemic period was from May to July, with a peak in May. Thus, SFTS reminds a significant public health problem, and development of prophylactic vaccines and effective antiviral drugs will be highly needed.展开更多
文摘严重发热伴血小板减少综合征(SFTS,severe fever and thrombocytopenia syndrome)是由一种新型布尼亚病毒SFTS病毒(severe fever and thrombocytopenia syndrome Virus,SFTSV)引起的传染病。由于SFTS的病死率可高达30%,因此需要对SFTS进行有效的针对性治疗。近年来针对SFTS抗病毒药物的研究表明,利巴韦林和法维吡拉韦的疗效较好。目前在体外试验和动物模型中,法维吡拉韦对SFTS的疗效明显高于利巴韦林。其它的药物,包括六氯酚、钙通道阻滞剂、2′-氟-2′-脱氧胞苷、咖啡酸、阿莫地喹和干扰素也有效的对SFTSV的增殖起到抑制作用。研究显示钙通道阻滞剂硝苯地平除了在体内、外的疗效外,还能降低临床病死率。本文对以上几种抗SFTSV药物研究进行了总结,并讨论抗SFTSV药物开发的最新进展。
基金supported by the Tianjin Natural Science Foundation(No.17JCYBJC16600)the Opened Fund of the State Key Laboratory on Integrated Optoelectronics(No.IOSKL2015KF06)the National Natural Science Foundation for Youth(No.11704283)
文摘A single-mode-few-mode-thin-core-single-mode(SFTS) structure based optical fiber sensor is fabricated and experimentally studied. The sensing principle relies on the inter-modal interference. Since the core diameter of few-mode fiber(FMF) is larger than that of single-mode fiber(SMF), the FMF helps to allow more light to enter the cladding of thin-core fiber(TCF), which helps TCF to excite cladding modes. The interference between core and cladding modes in TCF occurs at the joint of lead-out SMF and TCF. Experimental results demonstrate a refractive index(RI) sensitivity of-103.34 nm/RIU and a temperature sensitivity of 0.05 nm/℃. The proposed sensor not only can measure temperature, but also can measure RI. In addition, the proposed sensor is simple for without complicated fabrication process.
基金supported by the National Key R&D Program of China(2022YFF1203201)National Natural Science Foundation of China(82072295)Beijing Research Center for Respiratory Infectious Diseases Project(BJRID2024-009).
文摘Given the overlapping endemic regions and clinical similarities between severe fever with thrombocytopenia syndrome(SFTS)and hemorrhagic fever with renal syndrome(HFRS),we developed a dual real-time fluorescence-based reverse transcription quantitative polymerase chain reaction(RT-qPCR)method.Recombinant plasmids and synthetic ribonucleic acid(RNA)were constructed to evaluate the specificity,sensitivity and reproducibility of the assay.Additionally,we assessed the specificity of the assay using samples from three distinct groups:individuals with confirmed severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection(n=10),influenza A-positive individuals(n=10),and healthy controls.Receiver operating characteristic(ROC)curves were used to assess diagnostic accuracy,while the Kappa coefficient and linear regression analysis were employed to evaluate clinical applicability.Our method exhibited specificity for both SFTSV and Hantaan virus detection,with detection limits of 333 and 1,022 copies/mL using plasmids,and 1,247 and 898 copies/mL using synthetic RNA,respectively.We evaluated 100 clinical samples from each of SFTS and HFRS.The Kappa coefficients for both diseases were 0.96.The areas under the ROC curves were 0.991(P<0.001)and 0.989(P<0.001),respectively.The linear regression equations were as follows:log(y)=0.19+0.99 log(x)(R^(2)=0.95)for SFTS virus,and log(y)=0.01+0.65 log(x)(R^(2)=0.92)for Hantaan virus.We established an in-house RT-qPCR method for the rapid quantification of both pathogens,making it an ideal tool for early clinical differentiation.
基金supported by the National Major Science and Technol-ogy Project of China(2018ZX10711001,2016ZX10004222-002)。
文摘SFTS virus(SFTSV)is a novel bunyavirus,which was discovered as the etiological agent of severe fever with thrombocytopenia syndrome(SFTS)in China in 2009,and was now prevalent in at least 25 provinces in China.SFTS was subsequently identified in South Korea and Japan in 2012.To explore themolecular evolution and genetic characteristics of this newly identified pathogen,we reported 72 whole genome sequences of SFTSV,and built a dataset of SFTSV genome sequences containing 292 L-segment,302 M-segment and 502 S-segment.We clearly divided SFTSV into six genotypes,Genotype A-F.It was found that genotype F was the dominant epidemic genotype of Japan,South Korea,and Zhejiang province of China.The coalescent analysis supported that SFTSV originated in the early 18th century from Zhejiang province,and Genotype F was the most primitive one.Henan,Hubei,and Anhui provinces which are located in Dabie Mountain area weremainly epidemic of Genotype A,which emerged relatively late but distributed widely.A total of 37 recombination events were identified,making SFTSV with a high recombination frequency(L segment 5.1%,Msegment 3.6%,S segment 0.8%)among negative-strand segmented RNA viruses.It was identified that 19 reassortant strains belonged to 12 reassortment forms of SFTSV genome containing 6 newly identified forms.The reassortment virus and recombination in tick were both found for the first time.We also found many of genotype-specific mutation sites,7 of which could be considered as potential molecular marker for genotype classification.This study promoted a more comprehensive understanding of the phylogeny and origin,and the genetic diversity of SFTSV,and it could help the studies of other newly discovered tick-borne bunyavirus as reference data and research ideas.
基金This study was supported by the National Major Science and Technology Project of China(2018ZX10711001 and 2013ZX09102029)The funders had no role in study design,data collection and analysis,decision to publish,or preparation of the manuscript.
文摘Severe fever with thrombocytopenia syndrome(SFTS),caused by a novel identified bunyavirus SFTS virus(SFTSV),was an emerging viral infectious disease that was firstly reported in China.There are no licensed vaccines and therapeutics against SFTSV currently.B‐Propiolactone(BPL)inactivated whole virions of SFTSV strain AH12 were prepared as experimental vaccine in different antigen dose with or without Al(OH)3 adjuvant.The experimental SFTS vaccine was a satisfying immunogen,which could efficiently trigger the development of high levels of SFTSV NP‐specific IgG antibodies and neutralizing antibodies against SFTSV Strain HB29 in BALB/c and C57/BL6 mice,and could induce SFTS virus‐specific cellular immune responses to a certain extent.A single dose of vaccine was immunogenically insufficient in BALB/c mice;the second and third dose resulted in significant boost in antibody response.The use of Al(OH)3 adjuvant resulted in higher antibody titers.The mediate‐dose of vaccine could induce as high and equivalent level of antibody titer as that of high‐dose.The experimental SFTS vaccine in mediate‐and high antigen dose with adjuvant resulted in solid protection of C57/BL6 mice against wild‐type SFTSV challenge with markedly accelerated virus clearance from blood and spleen compared with controls.The experimental SFTS vaccine prepared in this study could efficiently elicit virus specific humoral and cellular immune responses in both BALB/c and C57/BL6 mice,and could protect C57/BL6 mice against SFTS virus challenge.These results supplied evidence that inactivated vaccine was a promising vaccine candidate for the prevention of SFTSV infection.
基金supported by the National Natural Science Foundation of China(61801503).
文摘Code acquisition is the kernel operation for signal synchronization in the spread-spectrum receiver.To reduce the computational complexity and latency of code acquisition,this paper proposes an efficient scheme employing sparse Fourier transform(SFT)and the relevant hardware architecture for field programmable gate array(FPGA)and application-specific integrated circuit(ASIC)implementation.Efforts are made at both the algorithmic level and the implementation level to enable merged searching of code phase and Doppler frequency without incurring massive hardware expenditure.Compared with the existing code acquisition approaches,it is shown from theoretical analysis and experimental results that the proposed design can shorten processing latency and reduce hardware complexity without degrading the acquisition probability.
文摘目的分析2012-2014年临海市发热伴血小板减少综合征(severe fever with thrombocytopenia syndrome,SFTS)流行特点及病原学监测结果,为临海市SFTS防控策略的制定提供依据,同时为其他低度流行区的SFTS防控提供参考。方法应用描述流行病学方法分析临海市2012-2014年SFTS病例特征及病原学监测结果。结果2012年临海市首次报告SFTS,到2014年出现一次集中于局部地区的流行,发病率较2012年显著上升(P<0.05);病例发生主要集中在4-5月份,只有1个发病高峰;患者中男性4例,男性发病率为0.649/10万,女性5例,女性发病率为0.881/10万,男女SFTS发病率差异无统计学意义(P>0.05);患者年龄主要集中在50-70岁之间,占77.8%;职业以农民为主,占88.9%;报告单位以本地综合性医疗机构为主,占77.8%。就诊不及时及诊断延误是造成SFTS病死率较高的主要原因。病例分布及病原学监测表明临海市的SFTS疫情属于局部暴发流行,66.7%的患者均来自白水洋镇黄坦乡或有此地野外暴露史,在患者居住地周围采集到的蜱虫标本检测3组布尼亚病毒核酸阳性,说明存在自然疫源地。结论临海市SFTS疫情具有明显的地域性和季节性,2014年发病率有升高趋势,并且存在自然疫源地,高龄农民为高危人群,SFTSV仍为主要病原,就诊不及时及诊断的延误是造成SFTS病死率高的主要原因。
基金supported by grants from the China Mega-Project for Infectious Diseases (2012ZX10004207)the China-US Collaborative Program on Emerging and Re-emerging Infectious Diseases (S2012Y03)+1 种基金the key project of the Health Ministry of Hubei Province (JX5A06)the Hubei Provincial Outstanding Medical Academic Leader program
文摘Severe fever with thrombocytopenia syndrome （SFTS） is an emerging infectious disease caused by SFTS virus （SFTSV）. SFTSV is associated with a high mortality rate and has been reported in China, South Korea and Japan. SFTSV undergoes rapid changes owing to evolution, gene mutations, and reassortment between different strains of SFTSV. In this review, we summarize the recent cases and general properties of SFTS, focusing on the epidemiology, genetic diversity, clinical features, and diagnostics of SFTSV in China. From 2010 to October 2016, SFTS cases were reported in 23 provinces of China, with increased numbers yearly. Infection and death cases are mainly found in central China, where the Haemaphysalis Iongicornis ticks are spread. The national average mortality rate of SFTS infection was 5.3%, with higher risk to elder people. The main epidemic period was from May to July, with a peak in May. Thus, SFTS reminds a significant public health problem, and development of prophylactic vaccines and effective antiviral drugs will be highly needed.
