目的调查广西壮族自治区凤山县野鼠感染斑点热群立克次体(spotted fever group rickettsiae,SFGR)的状况,了解其在当地流行的种类。方法用鼠笼在广西河池市凤山县捕捉野鼠,根据形态学特征与运用分子生物学方法对鼠种鉴定。解剖动物后取...目的调查广西壮族自治区凤山县野鼠感染斑点热群立克次体(spotted fever group rickettsiae,SFGR)的状况,了解其在当地流行的种类。方法用鼠笼在广西河池市凤山县捕捉野鼠,根据形态学特征与运用分子生物学方法对鼠种鉴定。解剖动物后取其脾脏组织提取总DNA样本,采用半巢氏PCR从样本中扩增立克次体外膜蛋白A(ompA),检测野鼠携带的斑点热群立克次体。对扩得DNA片段测序,并对获得的DNA序列进行同源性与系统进化分析。结果共捕获7种105只野鼠,除青毛鼠之外,从板齿鼠、小泡巨鼠、卡氏小鼠、锡金小鼠、黑缘齿鼠和罗赛鼠中检测到斑点热群立克次体,总阳性率为23.8%。基于ompA基因序列分析,检测到的斑点热群立克次体为敬信立克次体、饶氏立克次体和西伯利亚立克次体。结论广西壮族自治区凤山县的野鼠携带3种斑点热群立克次体,为斑点热的自然疫源地。因此应加强当地的蜱传立克次体病的监测与防控。展开更多
Because of the low temperature, large waste gas volume, high pollutant content, and complicated compositions, waste heat recovery and emission gas treatment of sintering flue gas have always presented a challenge in t...Because of the low temperature, large waste gas volume, high pollutant content, and complicated compositions, waste heat recovery and emission gas treatment of sintering flue gas have always presented a challenge in the steel industry, and this issue has attracted widespread attention both locally and abroad. Recently, based on the first domestic pilot plant and demonstration project, Baosteel has performed a series of innovative research and development investigations on its sintering flue gas recirculating （SFGR） process,in which the system design and optimization, dioxin-related contaminant source suppression, ore matching structure optimization, wear- resistant design of cycling fans and pipelines, high-efficiency dust removal equipment, system control and stable operation strategy, flue gas mixing and switching control, circular hood sealing, oxygen content conditioning, recirculation sintering system process control, and model development have been studied, and a complete set of equipment and technology for the SFGR process has been initially developed. The investigation results suggest that the SFGR process can not only significantly reduce exhaust gas volume and pollutant emissions but also recover low-temperature waste heat and reduce the energy consumption of sintering; therefore, the overall technologies possess great value in energy savings ,pollution emission reduction, and sintering ore quality/yield improvement.展开更多
目的确认长角血蜱是否具有经卵传播斑点热群立克次体(Spotted fever group rickettsia,SFGR)新基因型Candidatus Rickettsia longicornii的能力。方法采集牛体表饱血长角血蜱,在实验室诱导产卵,并采用聚合酶链式反应检测蜱卵中Candidatu...目的确认长角血蜱是否具有经卵传播斑点热群立克次体(Spotted fever group rickettsia,SFGR)新基因型Candidatus Rickettsia longicornii的能力。方法采集牛体表饱血长角血蜱,在实验室诱导产卵,并采用聚合酶链式反应检测蜱卵中Candidatus R. longicornii核酸。扩增长角血蜱母体和蜱卵中Candidatus R. longicornii基因序列,分析同源性和遗传进化关系。结果共采集55只饱血长角血蜱(雌性成蜱),检测Candidatus R. longicornii核酸,21只阳性,阳性率为38.18%。共收集约2 500只长角血蜱蜱卵,分成50组检测Candidatus R. longicornii,6组阳性,蜱卵最低感染率为0.24%。经同源性分析,长角血蜱母体和蜱卵Candidatus R. longicornii基因序列与首次在韩国发现的蜱源ROK-HL727株Candidatus R. longicornii基因序列同源性均达到99.79%以上。母体和蜱卵2个Candidatus R. longicornii基因序列间同源性达到99.69%以上,在系统进化关系上均与ROK-HL727株基因序列处于同一个分支,且遗传关系较近。结论长角血蜱母体SFGR Candidatus R. longicornii基因型感染率较高,且可经卵传播该基因型。展开更多
文摘目的调查广西壮族自治区凤山县野鼠感染斑点热群立克次体(spotted fever group rickettsiae,SFGR)的状况,了解其在当地流行的种类。方法用鼠笼在广西河池市凤山县捕捉野鼠,根据形态学特征与运用分子生物学方法对鼠种鉴定。解剖动物后取其脾脏组织提取总DNA样本,采用半巢氏PCR从样本中扩增立克次体外膜蛋白A(ompA),检测野鼠携带的斑点热群立克次体。对扩得DNA片段测序,并对获得的DNA序列进行同源性与系统进化分析。结果共捕获7种105只野鼠,除青毛鼠之外,从板齿鼠、小泡巨鼠、卡氏小鼠、锡金小鼠、黑缘齿鼠和罗赛鼠中检测到斑点热群立克次体,总阳性率为23.8%。基于ompA基因序列分析,检测到的斑点热群立克次体为敬信立克次体、饶氏立克次体和西伯利亚立克次体。结论广西壮族自治区凤山县的野鼠携带3种斑点热群立克次体,为斑点热的自然疫源地。因此应加强当地的蜱传立克次体病的监测与防控。
文摘Because of the low temperature, large waste gas volume, high pollutant content, and complicated compositions, waste heat recovery and emission gas treatment of sintering flue gas have always presented a challenge in the steel industry, and this issue has attracted widespread attention both locally and abroad. Recently, based on the first domestic pilot plant and demonstration project, Baosteel has performed a series of innovative research and development investigations on its sintering flue gas recirculating （SFGR） process,in which the system design and optimization, dioxin-related contaminant source suppression, ore matching structure optimization, wear- resistant design of cycling fans and pipelines, high-efficiency dust removal equipment, system control and stable operation strategy, flue gas mixing and switching control, circular hood sealing, oxygen content conditioning, recirculation sintering system process control, and model development have been studied, and a complete set of equipment and technology for the SFGR process has been initially developed. The investigation results suggest that the SFGR process can not only significantly reduce exhaust gas volume and pollutant emissions but also recover low-temperature waste heat and reduce the energy consumption of sintering; therefore, the overall technologies possess great value in energy savings ,pollution emission reduction, and sintering ore quality/yield improvement.
文摘目的确认长角血蜱是否具有经卵传播斑点热群立克次体(Spotted fever group rickettsia,SFGR)新基因型Candidatus Rickettsia longicornii的能力。方法采集牛体表饱血长角血蜱,在实验室诱导产卵,并采用聚合酶链式反应检测蜱卵中Candidatus R. longicornii核酸。扩增长角血蜱母体和蜱卵中Candidatus R. longicornii基因序列,分析同源性和遗传进化关系。结果共采集55只饱血长角血蜱(雌性成蜱),检测Candidatus R. longicornii核酸,21只阳性,阳性率为38.18%。共收集约2 500只长角血蜱蜱卵,分成50组检测Candidatus R. longicornii,6组阳性,蜱卵最低感染率为0.24%。经同源性分析,长角血蜱母体和蜱卵Candidatus R. longicornii基因序列与首次在韩国发现的蜱源ROK-HL727株Candidatus R. longicornii基因序列同源性均达到99.79%以上。母体和蜱卵2个Candidatus R. longicornii基因序列间同源性达到99.69%以上,在系统进化关系上均与ROK-HL727株基因序列处于同一个分支,且遗传关系较近。结论长角血蜱母体SFGR Candidatus R. longicornii基因型感染率较高,且可经卵传播该基因型。
