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STEADY HEAT TRANSFER ANALYSIS AND PARAMETER OPTIMIZATION FOR MULTILAYER THERMAL INSULATIONS 被引量:2
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作者 闫长海 曲寿江 +3 位作者 孟松鹤 陈贵清 杜善义 刘国仟 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2006年第4期257-263,共7页
The energy equilibrium equation and discrete ordinate methods are combined to establish the one-dimensional steady heat transfer mathematical model of multi-layer thermal insulations (MTIs) in metallic thermal prote... The energy equilibrium equation and discrete ordinate methods are combined to establish the one-dimensional steady heat transfer mathematical model of multi-layer thermal insulations (MTIs) in metallic thermal protection systems. The inverse problem of heat transfer is solved by the genetic algorithm and data from the steady heat transfer experiment of fibrous thermal insulations. The density radiation attenuation coefficient, the albedo of fibrous thermal insulations and the surface emissivity of reflective screens are optimized. Finally, the one-dimensional steady heat transfer model of MTIs with optimized thermal physical parameters is verified by experimental data of the effective MTI conductivity. 展开更多
关键词 metallic thermal protection system steady heat transfer muhilayer thermal insulations genetic algorithm
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Calculation of high-temperature insulation parameters and heat transfer behaviors of multilayer insulation by inverse problems method 被引量:8
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作者 Huang Can Zhang Yue 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2014年第4期791-796,共6页
In the present paper, a numerical model combining radiation and conduction for porous materials is developed based on the finite volume method. The model can be used to investigate high-temperature thermal insulations... In the present paper, a numerical model combining radiation and conduction for porous materials is developed based on the finite volume method. The model can be used to investigate high-temperature thermal insulations which are widely used in metallic thermal protection systems on reusable launch vehicles and high-temperature fuel cells. The effective thermal conductivities(ECTs) which are measured experimentally can hardly be used separately to analyze the heat transfer behaviors of conduction and radiation for high-temperature insulation. By fitting the effective thermal conductivities with experimental data, the equivalent radiation transmittance, absorptivity and reflectivity, as well as a linear function to describe the relationship between temperature and conductivity can be estimated by an inverse problems method. The deviation between the calculated and measured effective thermal conductivities is less than 4%. Using the material parameters so obtained for conduction and radiation, the heat transfer process in multilayer thermal insulation(MTI) is calculated and the deviation between the calculated and the measured transient temperatures at a certain depth in the multilayer thermal insulation is less than 6.5%. 展开更多
关键词 Effective thermal conductivity Fibrous insulation Finite volume methodHeat transfer Multilayer thermal insulation RADIATION
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Comprehensive evaluation of the transformer oil-paper insulation state based on RF-combination weighting and an improved TOPSIS method 被引量:11
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作者 Fugen Song Shichao Tong 《Global Energy Interconnection》 EI CAS CSCD 2022年第6期654-665,共12页
The accurate identification of the oil-paper insulation state of a transformer is crucial for most maintenance strategies.This paper presents a multi-feature comprehensive evaluation model based on combination weighti... The accurate identification of the oil-paper insulation state of a transformer is crucial for most maintenance strategies.This paper presents a multi-feature comprehensive evaluation model based on combination weighting and an improved technique for order of preference by similarity to ideal solution(TOPSIS)method to perform an objective and scientific evaluation of the transformer oil-paper insulation state.Firstly,multiple aging features are extracted from the recovery voltage polarization spectrum and the extended Debye equivalent circuit owing to the limitations of using a single feature for evaluation.A standard evaluation index system is then established by using the collected time-domain dielectric spectrum data.Secondly,this study implements the per-unit value concept to integrate the dimension of the index matrix and calculates the objective weight by using the random forest algorithm.Furthermore,it combines the weighting model to overcome the drawbacks of the single weighting method by using the indicators and considering the subjective experience of experts and the random forest algorithm.Lastly,the enhanced TOPSIS approach is used to determine the insulation quality of an oil-paper transformer.A verification example demonstrates that the evaluation model developed in this study can efficiently and accurately diagnose the insulation status of transformers.Essentially,this study presents a novel approach for the assessment of transformer oil-paper insulation. 展开更多
关键词 Combined weight method Random forest algorithm insulation aging assessment Oil-paper insulation Time-domain eigenvalue
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Aging Effect on Characteristics of Oil Impregnated Insulation Paper for Power Transformers
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作者 Loai S. Nasrat Nesrine Kassem Nadia Shukry 《Engineering(科研)》 2013年第1期1-7,共7页
This paper deals with experimental investigations of the electrical and physical properties of oil impregnated insulation paper for power transformers at different temperatures. The ac breakdown voltage, tensile stren... This paper deals with experimental investigations of the electrical and physical properties of oil impregnated insulation paper for power transformers at different temperatures. The ac breakdown voltage, tensile strength and water content of insulation papers impregnated in mineral oil for different time periods were investigated. The effect of insulation paper thickness on the electrical and mechanical properties has also been studied. The results showed that the breakdown voltage and the tensile strength decreased with increasing the time of immersion of insulation paper in oil at room temperature, at 5℃ and at -12℃. Also, the thermal aging effect on the characteristic of insulation paper has been studied. It was found that high temperatures affect the breakdown voltage and the tensile strength to a great extent. 展开更多
关键词 insulation Paper Power TRANsfORMERS AGING BREAKDOWN Voltage TENSILE Strength Water Content
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Insulation Performance of CF3I-N2 Gas Mixtures as Alternative for SF6 in GIS/C-GIS 被引量:26
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作者 DENG Yunkun XIAO Dengming CHEN Jiong 《高电压技术》 EI CAS CSCD 北大核心 2013年第9期2288-2293,共6页
CF3I has been widely considered as a potential alternative for SF6,because it has low global warming potential(GWP)but an insulation capability that is 1.2 times greater than that of SF6.In this paper,the electron swa... CF3I has been widely considered as a potential alternative for SF6,because it has low global warming potential(GWP)but an insulation capability that is 1.2 times greater than that of SF6.In this paper,the electron swarm parameters of CF3I and its gas mixture with N2,including the effective ionization coefficient and electron drift velocity,are examined theoretically through the Boltzmann equation method in the condition of steady-state Townsend(SST)experiments.Based on the derived data of the limiting field strength of CF3I-N2gas mixture,taking into consideration of environmental aspects such as GWP,ozone depletion potential(ODP),liquefaction temperature and toxicity,we studied the possibility of applying the gas mixture as the insulation medium in gas-insulated switchgears(GIS)or cubic type gas-insulated switchgears(C-GIS).It is found that CF3I-N2gas mixtures contained 30%~70%CF3I perform comprehensively better than pure SF6and compressed N2,and especially in medium and low voltage environments,the boiling point of CF3I-N2gas mixture meets the domestic and global requirements in mid-low latitude regions.Therefore we conclude that the gas mixture is acceptable for replacing SF6as the insulation medium in C-GISs. 展开更多
关键词 sf6气体 C-GIS 混合气体 绝缘性能 气体绝缘开关装置 气体混合物 玻尔兹曼方程 地理信息系统
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Comparative analysis of online monitoring methods for transformer insulation performance of 330-750kV substation
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作者 子贡 董海鹰 任伟 《Journal of Measurement Science and Instrumentation》 CAS CSCD 2016年第1期71-77,共7页
The online-monitoring methods for insulation performance of current transformers of 330-750 kV substation are analyzed and compared.The effectiveness and availability of each method are discussed.Main features,advanta... The online-monitoring methods for insulation performance of current transformers of 330-750 kV substation are analyzed and compared.The effectiveness and availability of each method are discussed.Main features,advantages and disadvantages of each method and its corresponding standard are also described. 展开更多
关键词 continuous control insulation performance online monitoring SUBSTATION
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Copper Release Kinetics and Ageing of Insulation Paper in Oil-Immersed Transformers 被引量:3
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作者 Olena Sevastyanova Bogdan Pasalskiy Boris Zhmud 《Engineering(科研)》 2015年第8期514-529,共16页
The paper provides a general overview of chemical processes leading to the degradation of oil-paper insulation in oil-immersed electrical current transformers. Previous knowledge available in literature is complemente... The paper provides a general overview of chemical processes leading to the degradation of oil-paper insulation in oil-immersed electrical current transformers. Previous knowledge available in literature is complemented by new results placing a specific emphasis on the physicochemical factors which affect the copper release in the insulation oil and the oil oxidation kinetics. It is demonstrated that various ageing processes interact with each other, with one or another process dominating under specific conditions. Comprehensive but disjoint studies focusing on separate sub-processes may produce rather misleading results, and occasionally, lie behind rather irrelevant quality demands imposed on the insulating liquids. 展开更多
关键词 TRANsfORMER OIL Insulating OIL COPPER CORROSION PAPER DEGRADATION Ageing
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The Resuming Insulation Discharge Failure and Conductor Overheating Failure Judgment Method of SF6 Electrical Equipment
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作者 Xiansheng ZHUANG Zhi LI Chengji HUANG 《Energy and Power Engineering》 2009年第2期122-127,共6页
Through the long time track examination and disintegration to SF6 circuit breaker, we obtain the massive monitor data and massive pictures. The criteria of resuming insulation discharge failure conforming to CSO2/CH2S... Through the long time track examination and disintegration to SF6 circuit breaker, we obtain the massive monitor data and massive pictures. The criteria of resuming insulation discharge failure conforming to CSO2/CH2S>7, is quite broad to SO2 and the H2S concentration permission. Even if it reaches 100μL/L, it will not be in danger immediately to the safe operation of equipment. We may plan, arrange, and overhaul calmly. When obtaining the bare conductor overheating failure, it has not involved the resuming insulation. We may use the resuming insulation discharge failure criterion. 展开更多
关键词 sf6 conductor DISCHARGE overheated FAILURE METHOD
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Test and analysis of the influence of the transformer insulation resistance and dielectric loss
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作者 ZHANG Mengjie HU Shunbin +2 位作者 SUN JiaLong XU Hai ZHANG Ning 《International English Education Research》 2015年第10期65-68,共4页
In view of long-term transformer insulation resistance and dielectric loss measurement, measurement and according to the national standard formula conversion values are not completely consistent, in this paper, a vari... In view of long-term transformer insulation resistance and dielectric loss measurement, measurement and according to the national standard formula conversion values are not completely consistent, in this paper, a variety of transformers are studied, and the applicable scope of the conversion formula is discussed emphatically, combined with the test results are analyzed of transformer insulation resistance value to temperature and oiliness and dielectric loss tangent to temperature and oiliness, and puts forward suggestions on how to improve the insulation performance of transformer. 展开更多
关键词 110kV transformer dielectric loss insulating resistor MEASUREMENT
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Overvoltage and Insulation Coordination for Valve Winding of ±800 kV UHVDC Converter Transformer 被引量:5
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作者 ZHOU Peihong DAI Ming HE Huiwen 《高电压技术》 EI CAS CSCD 北大核心 2012年第12期3146-3155,共10页
关键词 直流换流变压器 绝缘配合 过电压 特高压 转阀 绕组 绝缘水平 直流输电工程
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Superelastic and Washable Micro/Nanofibrous Sponges Based on Biomimetic Helical Fibers for Efficient Thermal Insulation
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作者 Fengjin Yang Zhifei Wang +7 位作者 Wei Zhang Sai Wang Yi‑Tao Liu Fei Wang Roman ASurmenev Jianyong Yu Shichao Zhang Bin Ding 《Nano-Micro Letters》 2026年第2期170-182,共13页
Extreme cold weather seriously harms human thermoregulatory system,necessitating high-performance insulating garments to maintain body temperature.However,as the core insulating layer,advanced fibrous materials always... Extreme cold weather seriously harms human thermoregulatory system,necessitating high-performance insulating garments to maintain body temperature.However,as the core insulating layer,advanced fibrous materials always struggle to balance mechanical properties and thermal insulation,resulting in their inability to meet the demands for both washing resistance and personal protection.Herein,inspired by the natural spring-like structures of cucumber tendrils,a superelastic and washable micro/nanofibrous sponge(MNFS)based on biomimetic helical fibers is directly prepared utilizing multiple-jet electrospinning technology for high-performance thermal insulation.By regulating the conductivity of polyvinylidene fluoride solution,multiple-jet ejection and multiple-stage whipping of jets are achieved,and further control of phase separation rates enables the rapid solidification of jets to form spring-like helical fibers,which are directly entangled to assemble MNFS.The resulting MNFS exhibits superelasticity that can withstand large tensile strain(200%),1000 cyclic tensile or compression deformations,and retain good resilience even in liquid nitrogen(-196℃).Furthermore,the MNFS shows efficient thermal insulation with low thermal conductivity(24.85 mW m^(-1)K^(-1)),close to the value of dry air,and remains structural stability even after cyclic washing.This work offers new possibilities for advanced fibrous sponges in transportation,environmental,and energy applications. 展开更多
关键词 ELECTROSPINNING Micro/nanofibrous sponge Hierarchical structure SUPERELASTICITY Thermal insulation
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基于改进Transformer结构的电力绝缘子运动模糊图像复原网络 被引量:1
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作者 李鹏 常乐 +2 位作者 覃发富 孟庆伟 陈继明 《电网技术》 北大核心 2025年第6期2623-2631,I0143-I0146,共13页
针对高压输电线路巡检航拍过程中产生的电力绝缘子图像运动模糊的失真情形,影响后续绝缘子定位及缺陷检测的问题,提出了一种基于改进Transformer结构的电力绝缘子图像运动模糊复原方法。为了适应电力绝缘子航拍图像中全局与局部模糊的... 针对高压输电线路巡检航拍过程中产生的电力绝缘子图像运动模糊的失真情形,影响后续绝缘子定位及缺陷检测的问题,提出了一种基于改进Transformer结构的电力绝缘子图像运动模糊复原方法。为了适应电力绝缘子航拍图像中全局与局部模糊的复原需求,在Transformer网络结构上引入条带注意力模块,结合卷积神经网络,在减小内存空间需求和不依赖大量训练数据的同时实现高效的模糊绝缘子图像复原;同时,在网络目标函数中引入对比学习损失,充分地挖掘和利用清晰与模糊电力绝缘子图像的关联信息。构建运动模糊绝缘子图像数据集进行图像复原与缺陷检测实验,结果表明,该文的运动模糊绝缘子图像复原方法在峰值信噪比(peak signal-to-noise ratio,PSNR)和结构相似度(structure similarity index measure,SSIM)这两个指标上均高于Deblur GAN-v2、MIMO-UNet等主流算法,使用目标检测算法YOLOv5和YOLOv7对去模糊前后的绝缘子进行定位与自爆缺陷检测后显示该文方法在提升高压输电线路巡检任务中绝缘子定位与缺陷检测的准确率上具有实际应用意义。 展开更多
关键词 运动模糊图像复原 TRANsfORMER 对比学习 绝缘子及缺陷检测
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心血管病高危人群健康效用测量工具的选择:EQ-5D-5L与SF-6Dv2的应用验证
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作者 孙菊 郭倩 +3 位作者 李浩淼 姚强 祝淑珍 李俊琳 《中国卫生政策研究》 北大核心 2025年第8期20-28,共9页
目的:以我国心血管病(Cardiovascular Diseases,CVD)高危人群筛查与干预项目为应用场景,系统评估EQ-5D-5L和SF-6Dv2量表在心血管病高危人群中的适用性。方法:采用Spearman相关系数比较两量表聚合效度;采用组内相关系数(ICC)、Bland-Alt... 目的:以我国心血管病(Cardiovascular Diseases,CVD)高危人群筛查与干预项目为应用场景,系统评估EQ-5D-5L和SF-6Dv2量表在心血管病高危人群中的适用性。方法:采用Spearman相关系数比较两量表聚合效度;采用组内相关系数(ICC)、Bland-Altman图比较一致性,并采用多元线性回归分析探讨两量表效用差异的影响因素;采用Kruskal-Wallis检验和t检验比较区分度;采用效应量(ES)、相对效率(RE)和ROC曲线下面积(ROC-AUC)比较灵敏度;比较天花板效应与地板效应。结果:5415个CVD高危人群中,两量表聚合效度较好,但各维度相关性较差,且测量结果一致性水平较低(ICC=0.367)。两量表对健康状况的区分度均较好,但SF-6Dv2的灵敏度更优且天花板效应更轻微。结论:在测量心血管病高危人群健康效用值时,量表选择需审慎,尤其是针对风险级别较高群体,使用SF-6Dv2更合适。 展开更多
关键词 CVD EQ-5D-5L sf-6Dv2 健康效用值
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基于Sf9细胞培养表达鸭短喙侏儒综合征病毒VP2蛋白的工艺优化
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作者 甘辉群 王永娟 +3 位作者 吴双 傅宏庆 谭菊 刘明生 《畜牧与兽医》 北大核心 2025年第9期87-92,共6页
旨在获得高表达的鸭短喙侏儒综合征病毒VP2抗原蛋白。本试验通过对接毒与表达工艺中pH值、Sf9细胞密度、接毒量、收获时间、搅拌转速和溶氧值等参数进行优化,考察VP2重组杆状病毒的增殖情况,收获病毒液,测定病毒含量变化;同时,对培养物... 旨在获得高表达的鸭短喙侏儒综合征病毒VP2抗原蛋白。本试验通过对接毒与表达工艺中pH值、Sf9细胞密度、接毒量、收获时间、搅拌转速和溶氧值等参数进行优化,考察VP2重组杆状病毒的增殖情况,收获病毒液,测定病毒含量变化;同时,对培养物进行VP2蛋白SDS-PAGE和Western blot分析,并采用SDS-PAGE灰度扫描测定蛋白含量。结果:在pH值维持6.8~7.2,接毒密度为1.0×10^(6)~2.0×10^(6)个/mL,感染复数(MOI)值为0.01~0.1,收获时间为接种后5~7 d,转速100~140 r/min,溶氧度控制在40%~80%时,重组杆状病毒生长较好,含量较高,达10^(7.7)~10^(8.5) TCID_(50)/mL;均出现约65 kDa目的条带,蛋白表达量基本一致,达57μg/mL以上。根据优化的工艺参数在5 L反应器进行5批抗原生产验证,VP2蛋白表达量达58~63μg/mL,蛋白生产稳定,表达量高。 展开更多
关键词 鸭短喙侏儒综合征 重组杆状病毒 sf9细胞 接毒工艺 表达生产工艺
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Quantitative grading of insulation damage to distribution transformers caused by high-altitude electromagnetic pulse based on partial discharge detection 被引量:3
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作者 Feng Qin Simeng Li +3 位作者 Wei Chen Yuxiang Yang Xin Nie Wei Wu 《High Voltage》 2025年第4期1032-1042,共11页
Distribution transformers may experience insulation breakdown or degradation when exposed to the influences of high-altitude electromagnetic pulse(HEMP),thus posing significant threats and uncertainties to the safety ... Distribution transformers may experience insulation breakdown or degradation when exposed to the influences of high-altitude electromagnetic pulse(HEMP),thus posing significant threats and uncertainties to the safety and stable operations of power systems.Therefore,this study constructs a HEMP impact-test platform for distribution trans-formers,evaluates and analyses the development patterns of partial discharge(PD)under the action of different amplitude conduction environments,decomposes and identifies the types of damage defects,proposes a quantitative classification method for the degree of HEMP damage to distribution transformers based on PD detection.And reveals the damage mechanism of distribution transformers exposed to HEMP at different damage levels.The results show that distribution transformers undergo insulation breakdown when subjected to the influences of HEMP and that performance degradation often occurs before breakdown.As the amplitude of the HEMP environment increases,the degree of transformer damage continues to deteriorate,and both the amount of PD and the repetition rate of discharges exhibit a step-wise increase,with the increase in the repetition rate lagging behind that of the discharge amount.Based on the patterns of discharge quantity and repetition rate,transformer damage is categorised into three stages:undamaged,latent defect discharge,and severe defect discharge. 展开更多
关键词 power systemsthereforethis high altitude electromagnetic pulse partial discharge pd amplitude conduction environmentsdecomposes identifies t distribution transformers quantitative classification insulation damage partial discharge
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草地贪夜蛾Sf9细胞外泌体microRNA调控AcMNPV增殖的研究
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作者 田伟彬 张以农 +2 位作者 任菲菲 严寄铭 孙京臣 《华南农业大学学报》 北大核心 2025年第2期133-140,共8页
【目的】探究草地贪夜蛾Spodoptera frugiperda Sf9细胞外泌体microRNA(miRNA)对苜蓿银纹夜蛾核多角体病毒(Autographa californica multiple nucleopolyhedrovirus,AcMNPV)增殖的影响。【方法】通过不连续蔗糖质量分数梯度超速离心纯... 【目的】探究草地贪夜蛾Spodoptera frugiperda Sf9细胞外泌体microRNA(miRNA)对苜蓿银纹夜蛾核多角体病毒(Autographa californica multiple nucleopolyhedrovirus,AcMNPV)增殖的影响。【方法】通过不连续蔗糖质量分数梯度超速离心纯化外泌体,随后利用透射电镜观察和纳米颗粒跟踪分析技术对纯化产物进行颗粒直径分析。运用sRNA高通量测序技术筛选AcMNPV感染Sf9细胞后外泌体差异表达的miRNA并预测其潜在靶基因对应的生物学通路。通过转染模拟物(mimic)和病毒感染等细胞试验以及qPCR验证外泌体miRNA的差异表达,并检测差异表达miRNA——sfr-miR-1a-3p对AcMNPV增殖的影响。【结果】成功纯化Sf9细胞外泌体,且AcMNPV感染有效刺激Sf9细胞外泌体的分泌量增加。AcMNPV感染Sf9细胞72 h后,经与Rfam数据库比对,筛选出11个差异表达的外泌体miRNAs,其中,8个miRNAs的转录水平与测序结果趋势一致。通过预测miRNAs靶基因和KEGG富集分析,发现潜在的靶基因主要富集在cAMP信号通路、PI3K-Akt信号通路、癌症通路、人乳头瘤病毒信号通路等,这表明差异表达外泌体miRNA可能参与昆虫天然免疫反应。过表达sfrmiR-1a-3p能显著提高AcMNPV病毒vp39基因的表达。【结论】本研究通过sRNA测序筛选了AcMNPV感染后Sf9细胞外泌体的差异表达miRNA,证明了AcMNPV通过促进外泌体分泌,协助被感染细胞传递miRNA−sfr-miR-1a-3p来影响周围未感染细胞,以促进自身增殖。以上结果为昆虫外泌体传递miRNA以调控病毒增殖的机制研究提供了重要的理论依据。 展开更多
关键词 草地贪夜蛾 sf9细胞 苜蓿银纹夜蛾核多角体病毒 外泌体 microRNA sfr-miR-1a-3p
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SF-1谱系垂体腺瘤组织中miRNA-133、FOXC1表达水平与临床特征及预后的关系
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作者 董晓柳 董伟 +2 位作者 张利 张欢 张于 《脑与神经疾病杂志》 2025年第11期707-712,共6页
目的探讨类固醇生成因子-1(SF-1)谱系垂体腺瘤组织中微小RNA-133(miRNA-133)、叉头框C1(FOXC1)表达水平与临床特征及预后的关系。方法选取2020年4月至2023年2月唐山市人民医院神经外科收治的116例垂体腺瘤患者。依据入组患者预后情况,... 目的探讨类固醇生成因子-1(SF-1)谱系垂体腺瘤组织中微小RNA-133(miRNA-133)、叉头框C1(FOXC1)表达水平与临床特征及预后的关系。方法选取2020年4月至2023年2月唐山市人民医院神经外科收治的116例垂体腺瘤患者。依据入组患者预后情况,分为预后良好组(n=85)与预后不良组(n=31)。用qRT-PCR测miRNA-133 mRNA、FOXC1 mRNA表达;荧光原位杂交和免疫组化法分别测miRNA-133、FOXC1表达。Pearson法分析两者相关性,多因素Logistic回归分析预后影响因素,受试者工作特征(ROC)曲线评估miRNA-133 mRNA、FOXC1 mRNA对预后的诊断价值。结果miRNA-133、FOXC1与肿瘤直径、侵袭性生长、临床分期相关(P<0.05)。分层回归显示,侵袭性生长负向影响miRNA-133 mRNA(P<0.05),侵袭性生长、临床分期正向影响FOXC1 mRNA(P<0.05)。Pearson分析表明,SF-1谱系垂体腺瘤组织中FOXC1 mRNA与miRNA-133 mRNA表达负相关(P<0.05)。多因素Logistic回归显示,侵袭性生长、miRNA-133 mRNA、FOXC1 mRNA水平是SF-1谱系垂体腺瘤患者预后不良独立危险因素。miRNA-133 mRNA、FOXC1 mRNA单独预测预后曲线下面积(AUC)为0.864、0.825,联合预测AUC为0.913,优于二者单独预测(Z二者联合=1.569,ZmiRNA-133 mRNA、二者联合=2.548,ZFOXC1 mRNA、二者联合=3.209,~均P<0.05)。结论SF-1谱系垂体腺瘤组织中miRNA-133表达水平显著降低,FOXC1表达水平显著升高,两者与临床特征及预后密切相关。 展开更多
关键词 sf-1谱系 垂体腺瘤 微小RNA-133 叉头框C1 临床特征
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TM9SF2缺失促进Ⅰ型干扰素信号通路抑制水疱性口炎病毒复制的初步研究
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作者 李康 王新宇 +5 位作者 叶然 郭凌云 王林旭 许诺 张彤 段小涛 《细胞与分子免疫学杂志》 北大核心 2025年第6期481-487,共7页
目的探究9次跨膜超家族蛋白2(TM9SF2)缺失对水疱性口炎病毒(VSV)复制的影响,并探究其参与抗病毒先天免疫的作用机制。方法采用转染小干扰RNA(siRNA)方法敲低人非小细胞肺癌细胞(A549)中TM9SF2基因;采用CCK-8法检测细胞增殖活性;建立VSV... 目的探究9次跨膜超家族蛋白2(TM9SF2)缺失对水疱性口炎病毒(VSV)复制的影响,并探究其参与抗病毒先天免疫的作用机制。方法采用转染小干扰RNA(siRNA)方法敲低人非小细胞肺癌细胞(A549)中TM9SF2基因;采用CCK-8法检测细胞增殖活性;建立VSV-绿色荧光蛋白(VSV-GFP)感染细胞模型;空斑实验检测病毒上清滴度;采用实时定量PCR和Western blot法检测VSV病毒感染A549细胞后病毒基因组复制的mRNA水平和蛋白水平表达情况,以及检测用双链RNA类似物聚胞苷酸〔poly(I∶C)〕刺激细胞后Ⅰ型干扰素信号通路中β干扰素(IFN-β)mRNA水平和干扰素调节因子3(IRF3)蛋白磷酸化水平。结果与阴性对照组相比,TM9SF2敲低效果显著;敲低TM9SF2后不影响A549细胞增殖;成功建立VSV-GFP感染A549细胞模型;在病毒刺激下,敲低TM9SF2后细胞荧光强度减弱,显著下调VSV的mRNA水平和蛋白水平,VSV的病毒滴度降低;在poly(I∶C)刺激下,敲低TM9SF2显著上调IFN-β的mRNA水平和IRF3蛋白磷酸化水平。结论TM9SF2缺失抑制VSV的复制,正向调控Ⅰ型干扰素信号通路,增强宿主抗病毒先天免疫反应。 展开更多
关键词 9次跨膜超家族蛋白2(TM9sf2) 水疱性口炎病毒(VSV) 病毒复制 抗病毒先天免疫
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外周血NLR、CAR、SF水平在评估小儿肺炎支原体肺炎病情严重程度及预后中的临床价值 被引量:4
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作者 郭宏丽 刘奇 +2 位作者 李诚 王红艳 李凯歌 《转化医学杂志》 2025年第1期10-14,41,共6页
目的 分析外周血中性粒细胞/淋巴细胞(NLR)、C反应蛋白/白蛋白比率(CAR)、铁蛋白(SF)水平在评估小儿肺炎支原体肺炎(MPP)病情严重程度及预后中的临床价值。方法 回顾性分析2021年1月至2024年3月西安交通大学附属红会医院儿科收治的104例... 目的 分析外周血中性粒细胞/淋巴细胞(NLR)、C反应蛋白/白蛋白比率(CAR)、铁蛋白(SF)水平在评估小儿肺炎支原体肺炎(MPP)病情严重程度及预后中的临床价值。方法 回顾性分析2021年1月至2024年3月西安交通大学附属红会医院儿科收治的104例MPP患儿的临床资料,并纳入MPP组,另选取同期体检健康儿童63名为健康对照组,比较2组外周血NLR、CAR、SF水平。依据临床肺部感染评分(CPIS)将MPP患儿分为轻症组(n=56)、中重症组(n=48),比较轻症组与中重症组的外周血NLR、CAR、SF水平和急性生理学与慢性健康状况Ⅱ(APACHEⅡ)评分。通过Pearson相关性分析外周血NLR、CAR、SF水平与APACHEⅡ评分相关性;以疗效判定标准分为预后不良组(n=69)和预后良好组(n=35),比较2组上述指标的变化,并绘制受试者工作特征(ROC)曲线分析外周血NLR、CAR、SF水平分别诊断及联合预测MPP患儿预后不良的诊断效能。结果 MPP组的外周血NLR、CAR、SF水平高于健康对照组,差异有统计学意义(P <0.05);中重症组的外周血NLR、CAR、SF水平和APACHEⅡ评分均高于轻症组,差异有统计学意义(P <0.05);预后不良组的外周血NLR、CAR、SF水平和APACHEⅡ评分均高于预后良好组,差异有统计学意义(P <0.05);经Pearson相关性分析显示,MPP患儿外周血NLR、CAR、SF水平与APACHEⅡ评分呈正相关(r=0.813、0.798、0.808,P <0.001);对外周血NLR、CAR、SF相对表达水平及三者联合诊断因子做ROC曲线分析,曲线下面积(AUC)分别为0.882、0.883、0.854及0.897,预测MPP患儿预后的敏感度分别为82.86%、80.00%、77.14%及85.71%,特异度分别为92.75%、92.75%、94.20%及91.30%。结论 MPP患儿外周血NLR、CAR、SF水平明显高于健康儿童,且随着病情加重上述指标水平提高,与病情严重程度呈正相关性,三者联合预测MPP患儿预后不良的诊断效能高。 展开更多
关键词 外周血NLR 外周血CAR 外周血sf
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设备吸附剂对SF_6气体分解产物检测结果影响的试验研究 被引量:8
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作者 苏镇西 赵也 《高压电器》 CAS CSCD 北大核心 2013年第6期26-31,37,共7页
笔者结合吸附剂的吸附原理,搭建了吸附剂试验平台,设计了试验流程,完成了大量试验,旨在探索设备中吸附剂对气体分解产物定量检测结果的影响。文中主要研究了吸附剂对分解产物的吸附影响,通过试验研究,确定了影响SF6电气设备中分解产物... 笔者结合吸附剂的吸附原理,搭建了吸附剂试验平台,设计了试验流程,完成了大量试验,旨在探索设备中吸附剂对气体分解产物定量检测结果的影响。文中主要研究了吸附剂对分解产物的吸附影响,通过试验研究,确定了影响SF6电气设备中分解产物检测结果的主要因素,包括容器材质、吸附剂种类、吸附剂用量、吸附剂安装位置等,并比较了相同条件下各种气体分解产物的吸附速率。文中结论对通过分解产物检测判断开关设备运行状况有重要指导意义。 展开更多
关键词 sf6气体 sf6气体绝缘设备 吸附剂 sf6气体分解产物
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