We assessed the quorum sensing(QS)inhibitory impact of sesamol against the foodborne bacterium Pseudomonas aeruginosa.At concentrations ranging from 50 to 200μg/mL,sesamol significantly inhibited the production of vi...We assessed the quorum sensing(QS)inhibitory impact of sesamol against the foodborne bacterium Pseudomonas aeruginosa.At concentrations ranging from 50 to 200μg/mL,sesamol significantly inhibited the production of virulence factors such as protease,elastase,pyocyanin,rhamnolipid,and chemotaxis,and improved the susceptibility of bacterial and biofilm cells to colistin.Integrated transcriptomics,metabolomics,and docking analyses indicated that exposure to sesamol destroyed the QS system and down-regulated the expressions of genes encoding virulence and antioxidant enzymes.The down-regulation of genes encoding antioxidant enzymes intensified oxidative stress,as demonstrated by the enhancement of reactive oxygen species and H_(2)O_(2).The enhanced oxidative stress changed the components of the cell membrane,improved its permeability,and ultimately enhanced the susceptibility of bacterial and biofilm cells to colistin.Moreover,exposure to sesamol also led to the disorder of amino acid metabolism and energy metabolism,eventually attenuating the pathogenicity of P.aeruginosa.These findings indicated that sesamol can function as a potent anti-virulence agent to defend against food spoilage caused by P.aeruginosa.展开更多
Objective: To investigate the antimelanogenesis properties of three sesame compoundssesamol, sesamin and sesamolin via two stages of melanin synthesis vis-à-vis sunscreen function and enzyme inhibition in melanom...Objective: To investigate the antimelanogenesis properties of three sesame compoundssesamol, sesamin and sesamolin via two stages of melanin synthesis vis-à-vis sunscreen function and enzyme inhibition in melanoma cell line in order to search for alternative depigmenting agents.Methods: Antimelanogenic effects of sesame lignans were assessed in SK-MEL2 compared with the reference depigmenting agents, kojic acid and b-arbutin, in order to evaluate:(a) the sunscreen function of sesamol, sesamin and sesamolin by measurement of UV absorbtion property;(b) the inhibition of tyrosinase activity through mushroom and cellular tyrosinase; and(c) the effect on melanin content and melanogenic protein expression(tyrosinase, TRP-1 and TRP-2) by Western blot analysis; and(d) the toxicity of sesamol, sesamin and sesamolin to cells using cell cytotoxicity assay.Results: The results showed that sesamin, sesamolin and sesamol exerted satisfiable sunscreen function by absorbed UVB at 290 nm. Sesamol exhibited the highest inhibition of mushroom tyrosinase activity, but lipophilic sesamolin exhibited the highest cellular tyrosinase inhibition(IC_(50) of 1.6 μM) followed by sesamin, sesamol, and kojic acid,respectively. The order from high to low inhibition of melanin pigment was detected in the SK-MEL2 treated with sesamolin, sesamin, sesamol, kojic acid, and b-arbutin,respectively. Sesamolin and sesamin successfully inhibited cellular tyrosinase activity and respectively decreased TRP-1/TRP-2(36%/15%) and TRP-1 levels(16%), thereby inhibiting melanogenesis via antityrosinase activity. No cytotoxicity to SK-MEL2 or Vero(normal) cell lines was observed at the lignan concentrations that exerted an antimelanogenic effect.Conclusions: Three sesame lignans prevent melanin synthesis through 2 stages:(a) by blocking melanin-induction and(b) by interrupting melanogenic enzyme production. This study provides evidence that sesamol, sesamin and sesamolin are potential for antimelanogenesis agents.展开更多
Cyclophosphamide(CYL)is a chemotherapeutic medication commonly used in managing various malignancies like breast cancer or leukemia.Though,CYL has been documented to induce lung toxicity.Mechanism of CYL toxicity is t...Cyclophosphamide(CYL)is a chemotherapeutic medication commonly used in managing various malignancies like breast cancer or leukemia.Though,CYL has been documented to induce lung toxicity.Mechanism of CYL toxicity is through oxidative stress and the release of damage-associated molecular patterns(DAMPs).Sesamol(SES)is a natural antioxidant isolated from Sesamum indicum and its effect against CYL-induced lung toxicity is not studied yet.This study aims to inves-tigate whether SES could prevent any deleterious effects induced by CYL on lung using normal human lung cells,WI-38 cell line,without suppressing its efficacy.Cells were pretreated with SES and/or CYL for 24 h,then cell viability was estimated by MTS and trypan blue assays.The mode of cell death was determined by AO/EB staining.Additionally,caspase-3 level,oxidative stress,and inflammatory markers were evaluated by colorimetric and ELISA techniques.qRT-PCR was performed to evaluate RAGE,NF-κB,and Beclin-1 mRNA-expression.CYL-treated WI-38 cells developed a significantly increased cell death with enhanced oxidative and RAGE/NF-κb/Autophagy signaling,which were all attenuated after pretreatment with SES.Thus,we concluded that SES offered a protective role against CYL-induced lung injury via suppressing oxidative stress and RAGE/NF-κB/Autophagy signaling,which is a natural safe therapeutic option against CYL toxicities.展开更多
The antioxidant activity of sesamol derivatives and their drug delivery via fullerene were investigated. Fullerene can interact with sesamol derivatives, and their adsorptions were possible from the energetic viewpoin...The antioxidant activity of sesamol derivatives and their drug delivery via fullerene were investigated. Fullerene can interact with sesamol derivatives, and their adsorptions were possible from the energetic viewpoint. Adding the NH_2 group to sesamol can improve the sensitivity of sesamol toward fullerene surface. The NH_2 and OMe substitutions increase the antioxidant activity of sesamol. The results can also be used to select novel sesamol derivatives with higher antioxidant activity and higher drug delivery ability.展开更多
OBJECTIVE Highly electronegative L5 low-density lipoprotein(LDL),an atherogenic LDL,induces endothelial cell(EC)senescence and has been implicated in the progression of atherosclerosis.We examine whether sesamol,a nat...OBJECTIVE Highly electronegative L5 low-density lipoprotein(LDL),an atherogenic LDL,induces endothelial cell(EC)senescence and has been implicated in the progression of atherosclerosis.We examine whether sesamol,a natural organic compound and component of sesame oil,prevents EC senescence induced by electronegative LDL(L5)and to investigate the underlying mechanisms.METHODS Syrian hamsters,which have a LDL profile similar to that of humans,were fed a normal chow diet(control),a high-fat diet(HFD),or a HFD supplemented with the administration of 50 or 100mg·kg-1 sesamol via oral gavage(HFD+sesamol)for 16 weeks(n=10 per group).Among these groups,we compared plasma L5 levels and aortic endothelial senescence in the aortic arch.In vitro,we examined the effects of sesamol on human aortic endothelial cell(HAEC)senescence and signaling pathways induced by L5.RESULTS Hamsters in the HFD group had higher plasma L5 levels than did the HFD+sesamol groups or control group.Betagalactosidase(gal)staining showed that aortic endothelial senescence was markedly increased in the aortic arch of the HFD group but not in that of the HFD+sesamol groups when compared with the control group.In vitro,treatment of HAECs with sesamol(1-3mol·L-1)blocked L5-induced EC senescence in a dose-dependent manner.Sesamol also markedly inhibited the L5-induced phosphorylation of p38 MAPK and p53 activation and increased Mdm2 and phosphorylation of Akt.CONCLUSION The critical findings of this study suggest that sesamol may provide protection against atherosclerosis and the development of cardiovascular disease in humans.展开更多
Objective: To investigate biomolecular alteration of sesamol on human lung adenocarcinoma(SK-LU-1) cells compared with cisplatin using Fourier transform infrared microscopy(FTIR). Methods: Cytotoxicity of sesamol was ...Objective: To investigate biomolecular alteration of sesamol on human lung adenocarcinoma(SK-LU-1) cells compared with cisplatin using Fourier transform infrared microscopy(FTIR). Methods: Cytotoxicity of sesamol was investigated against SK-LU-1 cells by using neutral red. DNA fragmentation and the cell cycle analysis were determined by agarose gel electrophoresis and flow cytometry, respectively. The FTIR microscopy technique was applied to explore the changes in cellular biochemical compositions in cells treated with sesamol that the biochemical and biological assays cannot cover. The alkylating property was determined by 4-(4-nitrobenzyl)pyridine assay. Results: Sesamol and cisplatin exerted an antiproliferative effect at 48 h with respective IC50 values of 2.7 and 0.07 m M. Both induced apoptosis by causing DNA damage and accumulation of cell populations at sub-G1. FTIR microscopy and Principle Component Analysis clearly discriminated the sesamol-and cisplatin-treated cells from the untreated cells or control. A significant increase of total lipid content was found in cisplatin-treated cells. Conformational changes in the proteins secondary structure from the β-helix to the β-sheet were found in both sesamol-and cisplatin-treated cells, as well as significant reductions in relative DNA content of both compared to the control were observed, suggesting DNA damage. A shift in the peak position of DNA region provides insight on the DNA interactions. Conclusions: The non-alkylating effect of sesamol based on the nitrobenzyl pyridine assay delineates the non-covalent binding mode of sesamol on DNA. Hydrogen bonding is the binding mode of sesamol on DNA, while for cisplatin it was covalent and hydrogen bonding.展开更多
Horseradish peroxidase (HRP) is generally used as a label enzyme in enzyme immunoassay (EIA).The procedure used for HRP detection in EIA is critical for sensitivity and precision.This paper describes a novel fluorimet...Horseradish peroxidase (HRP) is generally used as a label enzyme in enzyme immunoassay (EIA).The procedure used for HRP detection in EIA is critical for sensitivity and precision.This paper describes a novel fluorimetric assay for horseradish peroxidase (HRP) using sesamol as substrate.The principle of the assay is as follow:sesamol (3,4-methylenedioxy phenol) is reacted enzymatically in the presence of hydrogen peroxide to produce dimeric sesamol.The dimer is fluorescent and can be detected sensitively at ex.347 nm,em.427 nm.The measurable range of HRP was 1.0×10-18 to 1.0×10-15 mol/assay,with a detection limit of 1.0×10-18 mol/assay.The coefficient of variation (CV,n=8) was examined at each point on the standard curve,with a mean CV percentage of 3.8%.This assay system was applied to thyroid stimulating hormone (TSH) EIA using HRP as the label enzyme.展开更多
Background:Inflammation is a complex process with significant implications for animal health.Sesamol(SEL),a phenolic lignan from Sesamum indicum L.,possesses various health benefits.Objective:To assess the anti-inflam...Background:Inflammation is a complex process with significant implications for animal health.Sesamol(SEL),a phenolic lignan from Sesamum indicum L.,possesses various health benefits.Objective:To assess the anti-inflammatory effects of SEL alone and in combination with celecoxib(CxB)and ketoprofen(KPN)in a broiler chick model of inflammation,and to explore the molecular interactions of SEL with cyclooxygenase(COx)enzymes using in silico analysis.Methods:Inflammation was induced in broiler chicks via sub-plantar injection of O.5%formaldehyde.Animals were treated orally with SEL(25 mg/kg,50 mg/kg,and 100 mg/kg),CXB(42 mg/kg),KPN(42 mg/kg),or combinations of SEL either with CXB or KPN.Paw licking frequency and edema size were measured.Computational docking studies were performed to investigate SEL's binding affinity to COX-1 and COX-2.Results:SEL significantly(P<0.05)and dose-dependently reduced paw licking frequency and edema size.The combination of SEL with CXB showed a greater anti-inflammatory effect than the combination with KPN.In silico analysis revealed a higher binding affinity of SEL for COx-2(-6.1 kcal/mol)compared to COX-1(-5.8 kcal/mol).Conclusion:SEL exhibits anti-inflammatory activity and demonstrates synergistic effects with CXB and KPN in vivo.These effects are likely mediated through interactions with both COx-1 and COx-2 enzymes,with a preference for COx-2.These findings suggest SEL as a potential therapeutic agent for inflammation.展开更多
Sesamol and sesamin inhibitα-glucosidase activity and may therefore be effective in treating type 2 diabetes,but their mechanisms remain unknown.Therefore,the inhibitory effects of sesamol and sesamin onα-glucosidas...Sesamol and sesamin inhibitα-glucosidase activity and may therefore be effective in treating type 2 diabetes,but their mechanisms remain unknown.Therefore,the inhibitory effects of sesamol and sesamin onα-glucosidase activity were analyzed by multispectral technique and molecular docking.Our findings indicated that the sesamol and sesamin had a significant influence onα-glucosidase activity,with IC_(50) values of 0.96 mM and 0.33 mM,respectively,and their inhibition types were non-competitive.Both sesamol and sesamin quenched the fluorescence by a static quenching mechanism.The results of three-dimensional(3D)fluorescence,circular dichroism(CD)spectroscopy and Fourier transform infrared spectrometer(FT-IR)showed that both sesamol and sesamin had certain effects on the conformation ofα-glucosidase.Molecular docking results revealed that both sesamol and sesamin could spontaneously bind toα-glucosidase and form hydrogen bonds with some of the amino acid residues of the enzyme.As a result,this study sheds new light on sesamol/sesamin as a potentialα-glucosidase inhibitor.展开更多
Senescence is a general and irreversible process which depends on both inherent(free radical and age)and external(Ultraviolet irradiation)factors.Antioxidants and other natural compounds like plant and plant products ...Senescence is a general and irreversible process which depends on both inherent(free radical and age)and external(Ultraviolet irradiation)factors.Antioxidants and other natural compounds like plant and plant products are widespread use for their medicinal and therapeutic values.The present study focuses on the role of sesamol which has been used to delay the effects of photoaging using model nematode Caenorhabditis elegans(C.elegan)by measuring the longest life,average life,reproductive capacity,and the variation of reactive oxygen of C.elegans under different stress conditions.The result showed that 200μg·mL^(−1)sesamol significantly extended the life of C.elegans,that is,the mean lifespan of the treatment groups were 43.3%longer than control group.Meanwhile,sesamol significantly prolonged the lifespan of C.elegans under heat stress,ultraviolet irradiation stress,and oxidative stress.Above all,sesamol could be used as potential antioxidant compounds which will be of greater significance for health-based research.展开更多
基金supported by grants from the National Natural Science Foundation of China(32000091)General Projects of Natural Science Research in Universities of Jiangsu Province(20KJB180019)Jiangsu Youth Talent Promotion Project(TJ-2021-066)。
文摘We assessed the quorum sensing(QS)inhibitory impact of sesamol against the foodborne bacterium Pseudomonas aeruginosa.At concentrations ranging from 50 to 200μg/mL,sesamol significantly inhibited the production of virulence factors such as protease,elastase,pyocyanin,rhamnolipid,and chemotaxis,and improved the susceptibility of bacterial and biofilm cells to colistin.Integrated transcriptomics,metabolomics,and docking analyses indicated that exposure to sesamol destroyed the QS system and down-regulated the expressions of genes encoding virulence and antioxidant enzymes.The down-regulation of genes encoding antioxidant enzymes intensified oxidative stress,as demonstrated by the enhancement of reactive oxygen species and H_(2)O_(2).The enhanced oxidative stress changed the components of the cell membrane,improved its permeability,and ultimately enhanced the susceptibility of bacterial and biofilm cells to colistin.Moreover,exposure to sesamol also led to the disorder of amino acid metabolism and energy metabolism,eventually attenuating the pathogenicity of P.aeruginosa.These findings indicated that sesamol can function as a potent anti-virulence agent to defend against food spoilage caused by P.aeruginosa.
基金2011 (#542800) and 2012 (#552900), Khon Kaen University Research Funding supported this project
文摘Objective: To investigate the antimelanogenesis properties of three sesame compoundssesamol, sesamin and sesamolin via two stages of melanin synthesis vis-à-vis sunscreen function and enzyme inhibition in melanoma cell line in order to search for alternative depigmenting agents.Methods: Antimelanogenic effects of sesame lignans were assessed in SK-MEL2 compared with the reference depigmenting agents, kojic acid and b-arbutin, in order to evaluate:(a) the sunscreen function of sesamol, sesamin and sesamolin by measurement of UV absorbtion property;(b) the inhibition of tyrosinase activity through mushroom and cellular tyrosinase; and(c) the effect on melanin content and melanogenic protein expression(tyrosinase, TRP-1 and TRP-2) by Western blot analysis; and(d) the toxicity of sesamol, sesamin and sesamolin to cells using cell cytotoxicity assay.Results: The results showed that sesamin, sesamolin and sesamol exerted satisfiable sunscreen function by absorbed UVB at 290 nm. Sesamol exhibited the highest inhibition of mushroom tyrosinase activity, but lipophilic sesamolin exhibited the highest cellular tyrosinase inhibition(IC_(50) of 1.6 μM) followed by sesamin, sesamol, and kojic acid,respectively. The order from high to low inhibition of melanin pigment was detected in the SK-MEL2 treated with sesamolin, sesamin, sesamol, kojic acid, and b-arbutin,respectively. Sesamolin and sesamin successfully inhibited cellular tyrosinase activity and respectively decreased TRP-1/TRP-2(36%/15%) and TRP-1 levels(16%), thereby inhibiting melanogenesis via antityrosinase activity. No cytotoxicity to SK-MEL2 or Vero(normal) cell lines was observed at the lignan concentrations that exerted an antimelanogenic effect.Conclusions: Three sesame lignans prevent melanin synthesis through 2 stages:(a) by blocking melanin-induction and(b) by interrupting melanogenic enzyme production. This study provides evidence that sesamol, sesamin and sesamolin are potential for antimelanogenesis agents.
基金This research did not receive any specific grant from funding agencies in the public,commercial,or not-for-profit sectors.
文摘Cyclophosphamide(CYL)is a chemotherapeutic medication commonly used in managing various malignancies like breast cancer or leukemia.Though,CYL has been documented to induce lung toxicity.Mechanism of CYL toxicity is through oxidative stress and the release of damage-associated molecular patterns(DAMPs).Sesamol(SES)is a natural antioxidant isolated from Sesamum indicum and its effect against CYL-induced lung toxicity is not studied yet.This study aims to inves-tigate whether SES could prevent any deleterious effects induced by CYL on lung using normal human lung cells,WI-38 cell line,without suppressing its efficacy.Cells were pretreated with SES and/or CYL for 24 h,then cell viability was estimated by MTS and trypan blue assays.The mode of cell death was determined by AO/EB staining.Additionally,caspase-3 level,oxidative stress,and inflammatory markers were evaluated by colorimetric and ELISA techniques.qRT-PCR was performed to evaluate RAGE,NF-κB,and Beclin-1 mRNA-expression.CYL-treated WI-38 cells developed a significantly increased cell death with enhanced oxidative and RAGE/NF-κb/Autophagy signaling,which were all attenuated after pretreatment with SES.Thus,we concluded that SES offered a protective role against CYL-induced lung injury via suppressing oxidative stress and RAGE/NF-κB/Autophagy signaling,which is a natural safe therapeutic option against CYL toxicities.
文摘The antioxidant activity of sesamol derivatives and their drug delivery via fullerene were investigated. Fullerene can interact with sesamol derivatives, and their adsorptions were possible from the energetic viewpoint. Adding the NH_2 group to sesamol can improve the sensitivity of sesamol toward fullerene surface. The NH_2 and OMe substitutions increase the antioxidant activity of sesamol. The results can also be used to select novel sesamol derivatives with higher antioxidant activity and higher drug delivery ability.
基金The project supported by the National Science Council of Cinese Taipei(NSC102-2320-B-039-058)China Medical University,Chinese Taipei(CMU102-N-02and CMU103-N-08)
文摘OBJECTIVE Highly electronegative L5 low-density lipoprotein(LDL),an atherogenic LDL,induces endothelial cell(EC)senescence and has been implicated in the progression of atherosclerosis.We examine whether sesamol,a natural organic compound and component of sesame oil,prevents EC senescence induced by electronegative LDL(L5)and to investigate the underlying mechanisms.METHODS Syrian hamsters,which have a LDL profile similar to that of humans,were fed a normal chow diet(control),a high-fat diet(HFD),or a HFD supplemented with the administration of 50 or 100mg·kg-1 sesamol via oral gavage(HFD+sesamol)for 16 weeks(n=10 per group).Among these groups,we compared plasma L5 levels and aortic endothelial senescence in the aortic arch.In vitro,we examined the effects of sesamol on human aortic endothelial cell(HAEC)senescence and signaling pathways induced by L5.RESULTS Hamsters in the HFD group had higher plasma L5 levels than did the HFD+sesamol groups or control group.Betagalactosidase(gal)staining showed that aortic endothelial senescence was markedly increased in the aortic arch of the HFD group but not in that of the HFD+sesamol groups when compared with the control group.In vitro,treatment of HAECs with sesamol(1-3mol·L-1)blocked L5-induced EC senescence in a dose-dependent manner.Sesamol also markedly inhibited the L5-induced phosphorylation of p38 MAPK and p53 activation and increased Mdm2 and phosphorylation of Akt.CONCLUSION The critical findings of this study suggest that sesamol may provide protection against atherosclerosis and the development of cardiovascular disease in humans.
基金supported by the Higher Education Research Promotion and National Research University Project of Thailand,Office of the Higher Education Commission,through the Food and Functional Food Research Cluster and Research and Development of Herbal Nutraceutics Subcluster of Khon Kaen University(FC 3.1.13 PhD and NRU 541057)
文摘Objective: To investigate biomolecular alteration of sesamol on human lung adenocarcinoma(SK-LU-1) cells compared with cisplatin using Fourier transform infrared microscopy(FTIR). Methods: Cytotoxicity of sesamol was investigated against SK-LU-1 cells by using neutral red. DNA fragmentation and the cell cycle analysis were determined by agarose gel electrophoresis and flow cytometry, respectively. The FTIR microscopy technique was applied to explore the changes in cellular biochemical compositions in cells treated with sesamol that the biochemical and biological assays cannot cover. The alkylating property was determined by 4-(4-nitrobenzyl)pyridine assay. Results: Sesamol and cisplatin exerted an antiproliferative effect at 48 h with respective IC50 values of 2.7 and 0.07 m M. Both induced apoptosis by causing DNA damage and accumulation of cell populations at sub-G1. FTIR microscopy and Principle Component Analysis clearly discriminated the sesamol-and cisplatin-treated cells from the untreated cells or control. A significant increase of total lipid content was found in cisplatin-treated cells. Conformational changes in the proteins secondary structure from the β-helix to the β-sheet were found in both sesamol-and cisplatin-treated cells, as well as significant reductions in relative DNA content of both compared to the control were observed, suggesting DNA damage. A shift in the peak position of DNA region provides insight on the DNA interactions. Conclusions: The non-alkylating effect of sesamol based on the nitrobenzyl pyridine assay delineates the non-covalent binding mode of sesamol on DNA. Hydrogen bonding is the binding mode of sesamol on DNA, while for cisplatin it was covalent and hydrogen bonding.
文摘Horseradish peroxidase (HRP) is generally used as a label enzyme in enzyme immunoassay (EIA).The procedure used for HRP detection in EIA is critical for sensitivity and precision.This paper describes a novel fluorimetric assay for horseradish peroxidase (HRP) using sesamol as substrate.The principle of the assay is as follow:sesamol (3,4-methylenedioxy phenol) is reacted enzymatically in the presence of hydrogen peroxide to produce dimeric sesamol.The dimer is fluorescent and can be detected sensitively at ex.347 nm,em.427 nm.The measurable range of HRP was 1.0×10-18 to 1.0×10-15 mol/assay,with a detection limit of 1.0×10-18 mol/assay.The coefficient of variation (CV,n=8) was examined at each point on the standard curve,with a mean CV percentage of 3.8%.This assay system was applied to thyroid stimulating hormone (TSH) EIA using HRP as the label enzyme.
文摘Background:Inflammation is a complex process with significant implications for animal health.Sesamol(SEL),a phenolic lignan from Sesamum indicum L.,possesses various health benefits.Objective:To assess the anti-inflammatory effects of SEL alone and in combination with celecoxib(CxB)and ketoprofen(KPN)in a broiler chick model of inflammation,and to explore the molecular interactions of SEL with cyclooxygenase(COx)enzymes using in silico analysis.Methods:Inflammation was induced in broiler chicks via sub-plantar injection of O.5%formaldehyde.Animals were treated orally with SEL(25 mg/kg,50 mg/kg,and 100 mg/kg),CXB(42 mg/kg),KPN(42 mg/kg),or combinations of SEL either with CXB or KPN.Paw licking frequency and edema size were measured.Computational docking studies were performed to investigate SEL's binding affinity to COX-1 and COX-2.Results:SEL significantly(P<0.05)and dose-dependently reduced paw licking frequency and edema size.The combination of SEL with CXB showed a greater anti-inflammatory effect than the combination with KPN.In silico analysis revealed a higher binding affinity of SEL for COx-2(-6.1 kcal/mol)compared to COX-1(-5.8 kcal/mol).Conclusion:SEL exhibits anti-inflammatory activity and demonstrates synergistic effects with CXB and KPN in vivo.These effects are likely mediated through interactions with both COx-1 and COx-2 enzymes,with a preference for COx-2.These findings suggest SEL as a potential therapeutic agent for inflammation.
基金supported by the National Key Research and Development Project of China(2021YFD2100303).
文摘Sesamol and sesamin inhibitα-glucosidase activity and may therefore be effective in treating type 2 diabetes,but their mechanisms remain unknown.Therefore,the inhibitory effects of sesamol and sesamin onα-glucosidase activity were analyzed by multispectral technique and molecular docking.Our findings indicated that the sesamol and sesamin had a significant influence onα-glucosidase activity,with IC_(50) values of 0.96 mM and 0.33 mM,respectively,and their inhibition types were non-competitive.Both sesamol and sesamin quenched the fluorescence by a static quenching mechanism.The results of three-dimensional(3D)fluorescence,circular dichroism(CD)spectroscopy and Fourier transform infrared spectrometer(FT-IR)showed that both sesamol and sesamin had certain effects on the conformation ofα-glucosidase.Molecular docking results revealed that both sesamol and sesamin could spontaneously bind toα-glucosidase and form hydrogen bonds with some of the amino acid residues of the enzyme.As a result,this study sheds new light on sesamol/sesamin as a potentialα-glucosidase inhibitor.
基金supported by the earmarked fund for China Agriculture Research System(CARS-14-1-30).
文摘Senescence is a general and irreversible process which depends on both inherent(free radical and age)and external(Ultraviolet irradiation)factors.Antioxidants and other natural compounds like plant and plant products are widespread use for their medicinal and therapeutic values.The present study focuses on the role of sesamol which has been used to delay the effects of photoaging using model nematode Caenorhabditis elegans(C.elegan)by measuring the longest life,average life,reproductive capacity,and the variation of reactive oxygen of C.elegans under different stress conditions.The result showed that 200μg·mL^(−1)sesamol significantly extended the life of C.elegans,that is,the mean lifespan of the treatment groups were 43.3%longer than control group.Meanwhile,sesamol significantly prolonged the lifespan of C.elegans under heat stress,ultraviolet irradiation stress,and oxidative stress.Above all,sesamol could be used as potential antioxidant compounds which will be of greater significance for health-based research.
