Objective:To explore the trend of detection and antimicrobial resistance of Serratia marcescens with different infection types for 7 consecutive years,to provide a reference for future studies for the control of S.mar...Objective:To explore the trend of detection and antimicrobial resistance of Serratia marcescens with different infection types for 7 consecutive years,to provide a reference for future studies for the control of S.marcescens infections and a rational selection of antibiotics.Methods:S.marcescens isolates were collected from 2014 to 2020,and the trend of detection and antimicrobial resistance were analyzed according to different types of infection.Results:For 7 consecutive years,the data showed that patients with S.marcescens infections were mainly from the intensive care unit(ICU)(384 isolates,40.98%),and the isolates recovered were mainly from sputum samples(743 isolates,79.30%).The number of isolated strains increased every year,and the average rate of detection ranged from 0.60%to 0.80%.The detection rate of S.marcescens with hospital-acquired infections(HAI)showed a downward trend and that of S.marcescens with colonization showed an upward trend.The detection rate of multidrug-resistant S.marcescens fluctuated between 8.33%–16.89%.The resistance rate of S.marcescens to piperacillin was 17.0%–29.06%and the resistance rate to piperacillin tazobactam was 2.95%–13.13%.For cephalosporin antibiotics,the resistance rates of S.marcescens to cefuroxime and cefazolin were>99%and the resistance rates to ceftazidime and cefepime were<13%.The resistance rate of S.marcescens to aminoglycoside antibiotics,especially amikacin,was the lowest.The resistance rate of S.marcescens with community-acquired infections(CAI)to carbapenems was higher than that with HAI and colonization.Conclusion:The different infection types of S.marcescens have different detection and epidemic trends.In addition,resistance to carbapenems is different across the strains.展开更多
Serratia is an opportunistic pathogenic bacterium of the Enterobacteriaceae family, occasionally responsible for epidemics of nosocomial infections in critical departments;in particular in neonatal intensive care unit...Serratia is an opportunistic pathogenic bacterium of the Enterobacteriaceae family, occasionally responsible for epidemics of nosocomial infections in critical departments;in particular in neonatal intensive care units Enterobacteriaceae form a large family of Gram-negative bacteria, which cause diseases of highly variable severity, due to distinct pathogenic mechanisms. This family is heterogeneous as it consists of about 30 genera of bacteria and more than 100 species. However, all these germs have in common their preferential location in the digestive system, some being part of the normal flora although they are also present in the environment. Several metabolic processes characterize this bacterial family. These include the ability to reduce nitrate to nitrite (for energy generation), ferment glucose, lack cytochrome oxidase, be aerobic or anaerobic, motile or immobile, … Enterobacteriaceae constitute more than 80% of the germs isolated in the laboratory: Escherichia, Salmonella, Shigella, Klebsiella, Enterobacter, Serratia, Proteus, Morganella and Yersinia are the rods most often found. Regarding nosocomial infection to Serratia, this bacterium colonizes the respiratory, digestive and urinary systems of patients, mainly responsible for bacteremia, infections of the lower respiratory tract, urinary and skin infections. Through our work, we report the epidemic experienced in the neonatal intensive care unit MOHAMMED VI university hospital, mother-child hospital MARRAKECH MOROCCO for three months from December at February 2023 interesting 30 newborns whose clinical presentation was different, the positive diagnosis was based on blood and geographical samples taken by the bacteriology department to isolate the offending germs, the therapeutic management of our patients consisted essentially of various hygiene measures in association with dual antibiotic therapy based on meropenem and amikacin. The evolution was marked by the death of 21 patients (70%) and the recovery of 9 patients (30%). The prognosis of infection by serratia remains bleak with high morbidity interest in prevention by respecting the rules of hygiene, which begins with hand disinfection.展开更多
We present the 3<sup>rd</sup> known case of a Serratia rubidaea urinary tract infection in a 49-year-old male with past medical history of bilateral lower extremity paraplegia who presented to the emergenc...We present the 3<sup>rd</sup> known case of a Serratia rubidaea urinary tract infection in a 49-year-old male with past medical history of bilateral lower extremity paraplegia who presented to the emergency room with fever, lethargy, and red tinted urine. The Serratia genus, of the family Enterobacteracia, in particular the species marcescens, are important causes of infection in humans, animals, and insects, however, until the mid-1950s, this was not the case, and the organism was considered non-pathogenic and frequently used in medical experiments. Serratia are facultatively anaerobic, gram-negative rods, the majority with peritrichous flagella primarily inhabiting soil, water, and plant surfaces. Serratia marcescens is now a known pathogen, however, less frequently isolated species, including Serratia rubidaea, are worthy of discussion, especially due to its characteristic red color and rarity. We aim to increase the awareness Serratia rubidaea including its presentation, inherent antimicrobial resistance, and treatment options.展开更多
从大豆土壤中分离纯化得到一株具有卵磷脂和乐果降解能力的菌株Yj1,对该菌株进行鉴定、生长条件优化、酶活性鉴定以及有机磷降解酶的分离纯化。结果表明,Yj1与Serratia marcescens WW4(CP003959.1)的16S r DNA相似度为99%。正交试验对...从大豆土壤中分离纯化得到一株具有卵磷脂和乐果降解能力的菌株Yj1,对该菌株进行鉴定、生长条件优化、酶活性鉴定以及有机磷降解酶的分离纯化。结果表明,Yj1与Serratia marcescens WW4(CP003959.1)的16S r DNA相似度为99%。正交试验对所需培养基进行优化,得到该菌株的最佳生长条件为甘露糖、蛋白胨和p H 8的组合。Yj1菌株在两种磷源条件下,菌株生长量均很低,但72 h内以大豆卵磷脂为磷源时的菌体生长情况优于乐果。以大豆卵磷脂为磷源时酸性磷酸酶、碱性磷酸酶与有机磷降解酶活性明显高于以乐果为磷源时的酶活,且72 h内碱性磷酸酶活性一直都高于酸性磷酸酶和有机磷降解酶。硫酸铵沉淀法结合阳离子交换层析成功从Yj1菌体中分离纯化了有机磷降解酶,SDS-PAGE结果显示纯化的蛋白为单一条带。且阳离子交换层析的提纯倍数是硫酸氨沉淀的5.303倍,硫酸氨沉淀为粗酶的1.416倍。展开更多
基金Hospital Management Innovation Program of Jiangsu Hospital Association in 2021(Project No.:JSYGY-3-2021-511)。
文摘Objective:To explore the trend of detection and antimicrobial resistance of Serratia marcescens with different infection types for 7 consecutive years,to provide a reference for future studies for the control of S.marcescens infections and a rational selection of antibiotics.Methods:S.marcescens isolates were collected from 2014 to 2020,and the trend of detection and antimicrobial resistance were analyzed according to different types of infection.Results:For 7 consecutive years,the data showed that patients with S.marcescens infections were mainly from the intensive care unit(ICU)(384 isolates,40.98%),and the isolates recovered were mainly from sputum samples(743 isolates,79.30%).The number of isolated strains increased every year,and the average rate of detection ranged from 0.60%to 0.80%.The detection rate of S.marcescens with hospital-acquired infections(HAI)showed a downward trend and that of S.marcescens with colonization showed an upward trend.The detection rate of multidrug-resistant S.marcescens fluctuated between 8.33%–16.89%.The resistance rate of S.marcescens to piperacillin was 17.0%–29.06%and the resistance rate to piperacillin tazobactam was 2.95%–13.13%.For cephalosporin antibiotics,the resistance rates of S.marcescens to cefuroxime and cefazolin were>99%and the resistance rates to ceftazidime and cefepime were<13%.The resistance rate of S.marcescens to aminoglycoside antibiotics,especially amikacin,was the lowest.The resistance rate of S.marcescens with community-acquired infections(CAI)to carbapenems was higher than that with HAI and colonization.Conclusion:The different infection types of S.marcescens have different detection and epidemic trends.In addition,resistance to carbapenems is different across the strains.
文摘Serratia is an opportunistic pathogenic bacterium of the Enterobacteriaceae family, occasionally responsible for epidemics of nosocomial infections in critical departments;in particular in neonatal intensive care units Enterobacteriaceae form a large family of Gram-negative bacteria, which cause diseases of highly variable severity, due to distinct pathogenic mechanisms. This family is heterogeneous as it consists of about 30 genera of bacteria and more than 100 species. However, all these germs have in common their preferential location in the digestive system, some being part of the normal flora although they are also present in the environment. Several metabolic processes characterize this bacterial family. These include the ability to reduce nitrate to nitrite (for energy generation), ferment glucose, lack cytochrome oxidase, be aerobic or anaerobic, motile or immobile, … Enterobacteriaceae constitute more than 80% of the germs isolated in the laboratory: Escherichia, Salmonella, Shigella, Klebsiella, Enterobacter, Serratia, Proteus, Morganella and Yersinia are the rods most often found. Regarding nosocomial infection to Serratia, this bacterium colonizes the respiratory, digestive and urinary systems of patients, mainly responsible for bacteremia, infections of the lower respiratory tract, urinary and skin infections. Through our work, we report the epidemic experienced in the neonatal intensive care unit MOHAMMED VI university hospital, mother-child hospital MARRAKECH MOROCCO for three months from December at February 2023 interesting 30 newborns whose clinical presentation was different, the positive diagnosis was based on blood and geographical samples taken by the bacteriology department to isolate the offending germs, the therapeutic management of our patients consisted essentially of various hygiene measures in association with dual antibiotic therapy based on meropenem and amikacin. The evolution was marked by the death of 21 patients (70%) and the recovery of 9 patients (30%). The prognosis of infection by serratia remains bleak with high morbidity interest in prevention by respecting the rules of hygiene, which begins with hand disinfection.
文摘We present the 3<sup>rd</sup> known case of a Serratia rubidaea urinary tract infection in a 49-year-old male with past medical history of bilateral lower extremity paraplegia who presented to the emergency room with fever, lethargy, and red tinted urine. The Serratia genus, of the family Enterobacteracia, in particular the species marcescens, are important causes of infection in humans, animals, and insects, however, until the mid-1950s, this was not the case, and the organism was considered non-pathogenic and frequently used in medical experiments. Serratia are facultatively anaerobic, gram-negative rods, the majority with peritrichous flagella primarily inhabiting soil, water, and plant surfaces. Serratia marcescens is now a known pathogen, however, less frequently isolated species, including Serratia rubidaea, are worthy of discussion, especially due to its characteristic red color and rarity. We aim to increase the awareness Serratia rubidaea including its presentation, inherent antimicrobial resistance, and treatment options.
文摘从大豆土壤中分离纯化得到一株具有卵磷脂和乐果降解能力的菌株Yj1,对该菌株进行鉴定、生长条件优化、酶活性鉴定以及有机磷降解酶的分离纯化。结果表明,Yj1与Serratia marcescens WW4(CP003959.1)的16S r DNA相似度为99%。正交试验对所需培养基进行优化,得到该菌株的最佳生长条件为甘露糖、蛋白胨和p H 8的组合。Yj1菌株在两种磷源条件下,菌株生长量均很低,但72 h内以大豆卵磷脂为磷源时的菌体生长情况优于乐果。以大豆卵磷脂为磷源时酸性磷酸酶、碱性磷酸酶与有机磷降解酶活性明显高于以乐果为磷源时的酶活,且72 h内碱性磷酸酶活性一直都高于酸性磷酸酶和有机磷降解酶。硫酸铵沉淀法结合阳离子交换层析成功从Yj1菌体中分离纯化了有机磷降解酶,SDS-PAGE结果显示纯化的蛋白为单一条带。且阳离子交换层析的提纯倍数是硫酸氨沉淀的5.303倍,硫酸氨沉淀为粗酶的1.416倍。
