Two novel hydroperoxylated Lycopodium alkaloids, 11alpha-hydroperoxyphlegmariurine B (1) and 7-hydroperoxyphlegmariurine B (2), along with a known compound, phlegmanurine B (3), were isolated from the total alkaloid f...Two novel hydroperoxylated Lycopodium alkaloids, 11alpha-hydroperoxyphlegmariurine B (1) and 7-hydroperoxyphlegmariurine B (2), along with a known compound, phlegmanurine B (3), were isolated from the total alkaloid fraction of the Chinese medicinal herb Huperzia serrata (Thunb.) Trev. Their structures and relative configurations were elucidated on the basis of spectroscopic analyses.展开更多
The genetic diversity and differentiation of 6 mud crab Scylla serrata populations from southeastern China are investigated using allozyme electrophoresis. The mean number of alleles per locus population is 1.3 ±...The genetic diversity and differentiation of 6 mud crab Scylla serrata populations from southeastern China are investigated using allozyme electrophoresis. The mean number of alleles per locus population is 1.3 ± 0.1; the percentage of polymorphic loci per population is 27.3; the observed heterozygosity ranges from (0.195±0.083) to ( 0.241±0.090) and the expected heterozygosity ranges from ( 0.105±0.043 ) to ( 0.131±0.047 ). The coefficient of gene differentiation among populations is low (Fst =0.032), indicating that only 3.2 % of the total genetic diversity comes from inter-population, while the remaining 96.8 % comes from intra-population differences. The genetic distance among populations is 0.000-0.008 (the average is 0.002). Gene flow among the populations is large (Nm = 7.56).Genetic structure is very similar among 6 Scylla serrata populations.展开更多
Huperzine V, a new Lycopodium alkaloid, was isolated from the whole plant of Huperzia serrata, and the absolute stereochemistry was determined by X-ray crystallographic analysis.
Boswellia serrata is a widely used herb in Indian systems of medicine and is well known for its potential medicinal properties.A chromatographic method was developed for the analysis and quanti cation of six boswellic...Boswellia serrata is a widely used herb in Indian systems of medicine and is well known for its potential medicinal properties.A chromatographic method was developed for the analysis and quanti cation of six boswellic acid marker compounds,i.e.,keto boswellic acid(1),3-O-Acetyl 11-keto-boswellic acid(2),-b Boswellic acid(3),-Boswellic acid(4),3-O-Acetyl--boswellic acid(5)and 3-O-Acetyl--boswellic acid b(6)in commercial herbal products containing B.serrata as an ingredient.Combining UPLCbwith Q-Tof-MS/MS makes the better identi cation of secondary metabolites and adulterants in the herbal formulations containing B.serrata in rapid time using fragmentation approach than the traditional approaches.In this study quanti cation of boswellic acids with UPLC-PDA method was performed as per the pharmacopeia guidelines.Furthermore,minor phytochemical constituenBoswellia serrata is a widely used herb in Indian systems of medicine and is well known for its potential medicinal properties.A chromatographic method was developed for the analysis and quantification of six boswellic acid marker compounds,i.e.,keto boswellic acid(1),3-O-Acetyl 11-keto b-boswellic acid(2),ɑ-Boswellic acid(3),b-Boswellic acid(4),3-O-Acetyl-ɑ-boswellic acid(5)and 3-O-Acetyl-b-boswellic acid(6)in commercial herbal products containing B.serrata as an ingredient.Combining UPLC with Q-Tof-MS/MS makes the better identification of secondary metabolites and adulterants in the herbal formulations containing B.serrata in rapid time using fragmentation approach than the traditional approaches.In this study quantification of boswellic acids with UPLC-PDA method was performed as per the pharmacopeia guidelines.Furthermore,minor phytochemical constituents were identified and characterized with the help of LC-Q-Tof-MS/MS fragmentation data and various isoforms of boswellic acids and tirucallic acids in B.serrata oleo-gum-resin extract were identified.ts were identi ed and characterized with the help of LC-Q-Tof-MS/MS fragmentation data and various isoforms of boswellic acids and tirucallic acids in B.serrata oleo-gum-resin extract were identi ed.展开更多
Huperzine W, a novel 14 carbons Lycopodium alkaloid, was isolated from the whole plant of Huperzia serrata, and its stucture was determined by spectroscopic analysis.
Apoptosis or programmed cell death plays an essential role in chemotherapy-induced tumor cell killing, and inducers of apoptosis are commonly used in cancer therapy. Treatment with Zelkova serrata extracts was perform...Apoptosis or programmed cell death plays an essential role in chemotherapy-induced tumor cell killing, and inducers of apoptosis are commonly used in cancer therapy. Treatment with Zelkova serrata extracts was performed in human gingival fibroblast （HGF）, mouth epidermoid carcinoma cell （KB）, lower gingival squamous cancer cell （YD38） and tongue mucoepidermoid carcinoma cells （YD15）. We observed that extract prepared from Zelkova serrata twig selectively inhibited proliferation of various oral cancer cells, but not normal gingival fibroblasts, in a dose-dependent manner. Caspase-8-mediated apoptosis was induced by treatment with the extract only in mouth epidermoid carcinoma and not in other types of cancer cells, including lower gingival squamous cell carcinoma. The selective apoptotic effect of Zelkova serrata twig extract in mouth epidermoid carcinoma was dependent on normal p53 status. Apoptosis was not remarkably induced by treatment with the extract in either lower gingival squamous or tongue mucoepidermoid carcinoma cells, both of which contain abnormalities of p53. Upon treatment with Zelkova serrata twig extract, mouth epidermoid carcinoma cells accumulated in S phase by activation of p21. These data indicate that Zelkova serrata twig extract exerted a cancer type-specific, p53-dependent apoptotic effect and disturbed the cell cycle, which suggests that herbal medicine could be a treatment for specific types of cancers.展开更多
The regulation of three biogenic amines over the reproductive neuroendoerine activity of the male Scylla serrata was investigated by in vivo injection and in vitro incubation. The testicular index, the ratio of the ma...The regulation of three biogenic amines over the reproductive neuroendoerine activity of the male Scylla serrata was investigated by in vivo injection and in vitro incubation. The testicular index, the ratio of the mature sections in testes, and the ratio of Type B cells in androgenic gland were taken as the quantitative indexes. The in vivo injections indicated that： 5-HT can significantly promote the testicular development and the secretion of the androgenic gland in S. serrata; DA can inhibit the testicular development, but no influence on the secretion of the androgenic gland was found; no significant difference was observed between the OA-injected group and the concurrent control group. In vitro incubations showed that： 5-HT can stimulate the secretion of the brain and the thoracic ganglia, thus accelerating that of the androgenic gland; however, neither OA nor DA showed any significant influence on the secretion of the brain and the thoracic ganglionic mass. As to the optic ganglia, the three biogenic amines hardly have any effect on its secretion. It is the first time to report the regulation of biogenic amines over the reproductive neuroendocrine of male crustaceans through vitro experiments. Results corroborate that 5-HT activates the brain and the thoracic ganglia to secret GSH first, then promote the testicular development through the activity of the androgenic gland.展开更多
文摘Two novel hydroperoxylated Lycopodium alkaloids, 11alpha-hydroperoxyphlegmariurine B (1) and 7-hydroperoxyphlegmariurine B (2), along with a known compound, phlegmanurine B (3), were isolated from the total alkaloid fraction of the Chinese medicinal herb Huperzia serrata (Thunb.) Trev. Their structures and relative configurations were elucidated on the basis of spectroscopic analyses.
基金This study was supported by the National Natural Science Foundation of China under contract No.40376044Marine“863"Item of China under contract No.2002AA603013Foundation of Jimei University.
文摘The genetic diversity and differentiation of 6 mud crab Scylla serrata populations from southeastern China are investigated using allozyme electrophoresis. The mean number of alleles per locus population is 1.3 ± 0.1; the percentage of polymorphic loci per population is 27.3; the observed heterozygosity ranges from (0.195±0.083) to ( 0.241±0.090) and the expected heterozygosity ranges from ( 0.105±0.043 ) to ( 0.131±0.047 ). The coefficient of gene differentiation among populations is low (Fst =0.032), indicating that only 3.2 % of the total genetic diversity comes from inter-population, while the remaining 96.8 % comes from intra-population differences. The genetic distance among populations is 0.000-0.008 (the average is 0.002). Gene flow among the populations is large (Nm = 7.56).Genetic structure is very similar among 6 Scylla serrata populations.
文摘Huperzine V, a new Lycopodium alkaloid, was isolated from the whole plant of Huperzia serrata, and the absolute stereochemistry was determined by X-ray crystallographic analysis.
基金Dr.S.Chandrasekhar,Director,CSIR-IICT,for the financial grant under MLP-0030CSIR for financial support(IICT Communication No.IICT/Pubs/2018/183gs5)
文摘Boswellia serrata is a widely used herb in Indian systems of medicine and is well known for its potential medicinal properties.A chromatographic method was developed for the analysis and quanti cation of six boswellic acid marker compounds,i.e.,keto boswellic acid(1),3-O-Acetyl 11-keto-boswellic acid(2),-b Boswellic acid(3),-Boswellic acid(4),3-O-Acetyl--boswellic acid(5)and 3-O-Acetyl--boswellic acid b(6)in commercial herbal products containing B.serrata as an ingredient.Combining UPLCbwith Q-Tof-MS/MS makes the better identi cation of secondary metabolites and adulterants in the herbal formulations containing B.serrata in rapid time using fragmentation approach than the traditional approaches.In this study quanti cation of boswellic acids with UPLC-PDA method was performed as per the pharmacopeia guidelines.Furthermore,minor phytochemical constituenBoswellia serrata is a widely used herb in Indian systems of medicine and is well known for its potential medicinal properties.A chromatographic method was developed for the analysis and quantification of six boswellic acid marker compounds,i.e.,keto boswellic acid(1),3-O-Acetyl 11-keto b-boswellic acid(2),ɑ-Boswellic acid(3),b-Boswellic acid(4),3-O-Acetyl-ɑ-boswellic acid(5)and 3-O-Acetyl-b-boswellic acid(6)in commercial herbal products containing B.serrata as an ingredient.Combining UPLC with Q-Tof-MS/MS makes the better identification of secondary metabolites and adulterants in the herbal formulations containing B.serrata in rapid time using fragmentation approach than the traditional approaches.In this study quantification of boswellic acids with UPLC-PDA method was performed as per the pharmacopeia guidelines.Furthermore,minor phytochemical constituents were identified and characterized with the help of LC-Q-Tof-MS/MS fragmentation data and various isoforms of boswellic acids and tirucallic acids in B.serrata oleo-gum-resin extract were identified.ts were identi ed and characterized with the help of LC-Q-Tof-MS/MS fragmentation data and various isoforms of boswellic acids and tirucallic acids in B.serrata oleo-gum-resin extract were identi ed.
文摘Huperzine W, a novel 14 carbons Lycopodium alkaloid, was isolated from the whole plant of Huperzia serrata, and its stucture was determined by spectroscopic analysis.
基金supported by Dankook University Research Grant (2011)
文摘Apoptosis or programmed cell death plays an essential role in chemotherapy-induced tumor cell killing, and inducers of apoptosis are commonly used in cancer therapy. Treatment with Zelkova serrata extracts was performed in human gingival fibroblast （HGF）, mouth epidermoid carcinoma cell （KB）, lower gingival squamous cancer cell （YD38） and tongue mucoepidermoid carcinoma cells （YD15）. We observed that extract prepared from Zelkova serrata twig selectively inhibited proliferation of various oral cancer cells, but not normal gingival fibroblasts, in a dose-dependent manner. Caspase-8-mediated apoptosis was induced by treatment with the extract only in mouth epidermoid carcinoma and not in other types of cancer cells, including lower gingival squamous cell carcinoma. The selective apoptotic effect of Zelkova serrata twig extract in mouth epidermoid carcinoma was dependent on normal p53 status. Apoptosis was not remarkably induced by treatment with the extract in either lower gingival squamous or tongue mucoepidermoid carcinoma cells, both of which contain abnormalities of p53. Upon treatment with Zelkova serrata twig extract, mouth epidermoid carcinoma cells accumulated in S phase by activation of p21. These data indicate that Zelkova serrata twig extract exerted a cancer type-specific, p53-dependent apoptotic effect and disturbed the cell cycle, which suggests that herbal medicine could be a treatment for specific types of cancers.
基金supported by the National Natural Science Foundation of China under contract No.30300269the National High Technology Research and Development Program("863"program)of China under contract No.2002AA603013.
文摘The regulation of three biogenic amines over the reproductive neuroendoerine activity of the male Scylla serrata was investigated by in vivo injection and in vitro incubation. The testicular index, the ratio of the mature sections in testes, and the ratio of Type B cells in androgenic gland were taken as the quantitative indexes. The in vivo injections indicated that： 5-HT can significantly promote the testicular development and the secretion of the androgenic gland in S. serrata; DA can inhibit the testicular development, but no influence on the secretion of the androgenic gland was found; no significant difference was observed between the OA-injected group and the concurrent control group. In vitro incubations showed that： 5-HT can stimulate the secretion of the brain and the thoracic ganglia, thus accelerating that of the androgenic gland; however, neither OA nor DA showed any significant influence on the secretion of the brain and the thoracic ganglionic mass. As to the optic ganglia, the three biogenic amines hardly have any effect on its secretion. It is the first time to report the regulation of biogenic amines over the reproductive neuroendocrine of male crustaceans through vitro experiments. Results corroborate that 5-HT activates the brain and the thoracic ganglia to secret GSH first, then promote the testicular development through the activity of the androgenic gland.
