Serglycin belongs to a family of small proteoglycans with Ser-Gly dipeptide repeats,and it is modified with different types of glycosaminoglycan side chains.Intracellular serglycin affects the retention and secretion ...Serglycin belongs to a family of small proteoglycans with Ser-Gly dipeptide repeats,and it is modified with different types of glycosaminoglycan side chains.Intracellular serglycin affects the retention and secretion of proteases,chemokines,or other cytokines by physically binding to these factors in secretory granules.Extracellular serglycin has been found to be released by several types of human cancer cells,and it is able to promote the metastasis of nasopharyngeal carcinoma cells.Serglycin can bind to CD44,which is another glycoprotein located in cellular membrane.Serglycin's function of promoting cancer cell metastasis depends on glycosylation of its core protein,which can be achieved by autocrine as well as paracrine secretion mechanisms.Further investigations are warranted to elucidate serglycin signaling mechanisms with the goal of targeting them to prevent cancer cell metastasis.展开更多
目的探究宫颈癌中丝甘蛋白聚糖(serglycin,SRGN)的表达水平与上皮-间质细胞转化(epithelial to mesenchy-mal transition,EMT)之间的关联性。方法采用生信软件GEO数据库预测SRGN在宫颈癌中的表达水平。收集70例宫颈癌患者的病理切片,通...目的探究宫颈癌中丝甘蛋白聚糖(serglycin,SRGN)的表达水平与上皮-间质细胞转化(epithelial to mesenchy-mal transition,EMT)之间的关联性。方法采用生信软件GEO数据库预测SRGN在宫颈癌中的表达水平。收集70例宫颈癌患者的病理切片,通过免疫组化二步法、直接免疫荧光法检测宫颈癌组织中SRGN蛋白的表达。通过转染技术、划痕实验、蛋白印迹实验,检测SRGN对Hela细胞迁移、EMT影响。结果GEO数据分析结果显示,SRGN在宫颈癌组织中表达明显高于正常组织,差异有统计学意义(P<0.05)。病理特征分析显示,SRGN与盆腔或腹主动脉旁淋巴结转移和TNM分期(Ⅲ、Ⅳ期)密切相关(P<0.001)。体外细胞实验显示,低表达SRGN抑制Hela细胞的迁移、EMT能力(P<0.01)。结论SRGN可能通过调节EMT进程促进宫颈癌细胞生长。展开更多
Esophageal squamous cell carcinoma(ESCC)is a highly malignant disease that has a poor prognosis.Its high lethality is mainly due to the lack of symptoms at early stages,which culminates in diagnosis at a late stage wh...Esophageal squamous cell carcinoma(ESCC)is a highly malignant disease that has a poor prognosis.Its high lethality is mainly due to the lack of symptoms at early stages,which culminates in diagnosis at a late stage when the tumor has already metastasized.Unfortunately,the common cancer biomarkers have low sensitivity and specificity in esophageal cancer.Therefore,a better understanding of the molecular mechanisms underlying ESCC progression is needed to identify novel diagnostic markers and therapeutic targets for intervention.The invasion of cancer cells into the surrounding tissue is a crucial step for metastasis.During metastasis,tumor cells can interact with extracellular components and secrete proteolytic enzymes to remodel the surrounding tumor microenvironment.Proteoglycans are one of the major components of extracellular matrix.They are involved in multiple processes of cancer cell invasion and metastasis by interacting with soluble bioactive molecules,surrounding matrix,cell surface receptors,and enzymes.Apart from having diverse functions in tumor cells and their surrounding microenvironment,proteoglycans also have diagnostic and prognostic significance in cancer patients.However,the functional significance and underlying mechanisms of proteoglycans in ESCC are not well understood.This review summarizes the proteoglycans that have been studied in ESCC in order to provide a comprehensive view of the role of proteoglycans in the progression of this cancer type.A long term goal would be to exploit these molecules to provide new strategies for therapeutic intervention.展开更多
基金supported by a grant from the State Key Program of National Natural Science Foundation of China (No. 81030043)
文摘Serglycin belongs to a family of small proteoglycans with Ser-Gly dipeptide repeats,and it is modified with different types of glycosaminoglycan side chains.Intracellular serglycin affects the retention and secretion of proteases,chemokines,or other cytokines by physically binding to these factors in secretory granules.Extracellular serglycin has been found to be released by several types of human cancer cells,and it is able to promote the metastasis of nasopharyngeal carcinoma cells.Serglycin can bind to CD44,which is another glycoprotein located in cellular membrane.Serglycin's function of promoting cancer cell metastasis depends on glycosylation of its core protein,which can be achieved by autocrine as well as paracrine secretion mechanisms.Further investigations are warranted to elucidate serglycin signaling mechanisms with the goal of targeting them to prevent cancer cell metastasis.
文摘目的探究宫颈癌中丝甘蛋白聚糖(serglycin,SRGN)的表达水平与上皮-间质细胞转化(epithelial to mesenchy-mal transition,EMT)之间的关联性。方法采用生信软件GEO数据库预测SRGN在宫颈癌中的表达水平。收集70例宫颈癌患者的病理切片,通过免疫组化二步法、直接免疫荧光法检测宫颈癌组织中SRGN蛋白的表达。通过转染技术、划痕实验、蛋白印迹实验,检测SRGN对Hela细胞迁移、EMT影响。结果GEO数据分析结果显示,SRGN在宫颈癌组织中表达明显高于正常组织,差异有统计学意义(P<0.05)。病理特征分析显示,SRGN与盆腔或腹主动脉旁淋巴结转移和TNM分期(Ⅲ、Ⅳ期)密切相关(P<0.001)。体外细胞实验显示,低表达SRGN抑制Hela细胞的迁移、EMT能力(P<0.01)。结论SRGN可能通过调节EMT进程促进宫颈癌细胞生长。
基金Supported by Research Grants Council of the Hong Kong SAR,China,No.17111016 and No.17100819.
文摘Esophageal squamous cell carcinoma(ESCC)is a highly malignant disease that has a poor prognosis.Its high lethality is mainly due to the lack of symptoms at early stages,which culminates in diagnosis at a late stage when the tumor has already metastasized.Unfortunately,the common cancer biomarkers have low sensitivity and specificity in esophageal cancer.Therefore,a better understanding of the molecular mechanisms underlying ESCC progression is needed to identify novel diagnostic markers and therapeutic targets for intervention.The invasion of cancer cells into the surrounding tissue is a crucial step for metastasis.During metastasis,tumor cells can interact with extracellular components and secrete proteolytic enzymes to remodel the surrounding tumor microenvironment.Proteoglycans are one of the major components of extracellular matrix.They are involved in multiple processes of cancer cell invasion and metastasis by interacting with soluble bioactive molecules,surrounding matrix,cell surface receptors,and enzymes.Apart from having diverse functions in tumor cells and their surrounding microenvironment,proteoglycans also have diagnostic and prognostic significance in cancer patients.However,the functional significance and underlying mechanisms of proteoglycans in ESCC are not well understood.This review summarizes the proteoglycans that have been studied in ESCC in order to provide a comprehensive view of the role of proteoglycans in the progression of this cancer type.A long term goal would be to exploit these molecules to provide new strategies for therapeutic intervention.
