Bathymodiolus mussels distribute in both deep-sea cold seep and hydrothermal vent environments,whose endosymbiotic gill tissue is a prominent character for the adaptation of extreme habitats.However,few studies explor...Bathymodiolus mussels distribute in both deep-sea cold seep and hydrothermal vent environments,whose endosymbiotic gill tissue is a prominent character for the adaptation of extreme habitats.However,few studies explored the adaptation mechanisms through comparative transcriptome sequencing and analysis of different tissues between seep mussels and vent mussels.We performed the comparative transcriptome sequencing and analysis for three tissue types(gill,mantle,and adductor muscle)of Bathymodiolus mussels collected from a cold seeping site Station S 11 and the 50-km away hydrothermal field Minami-Ensei Knoll in the Okinawa Trough.Results show that gene expression patterns had distinct tissue specificity.Compared with the non-endosymbiotic tissues(mantle and adductor muscle),the significantly strengthened gene functions in endosymbiotic gill included microbial recognition(fibrinogen C domain-containing protein 1-B-like(fibcd),fibrinogen-related protein 8(frp),peptidoglycan recognition proteins(pgrp),and C-type lectin(clec)),cell apoptosis and immunity(interferon regulatory factor 1/2-like 1(ir f),cathepsin D(ctsd),caspase 2(casp 2)),and antioxidant capacity(copper/zinc superoxide dismutase(czsod),glutathione peroxidase(gpx),selenoprotein(sel)),in both seep and vent individuals.Consistent with metal accumulation,high expression levels of genes related to heavy metal detoxification(cytochrome P 450(cyp),ferritin-like(ftl),metallothionein(mt),glutathione S-transferase(gst))were also observed in gill.Moreover,to adapt to high hydrostatic pressure in the deep sea,the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways associated with cellular community were significantly enriched in all three tissues,suggesting the regulation of cell structure and cell adhesion at transcriptional level.This study obtained gene expression profiles of deep-sea mussels subsisting at cold seep and hydrothermal vent sites,which could lay foundations for comprehensive investigations of molecular basis in adaptation of deep-sea mussels to the two extreme ecosystems.展开更多
Dissolved carbon(dissolved organic carbon and dissolved inorganic carbon)is the major component of the ocean carbon cycle,representing one of the largest carbon pools on Earth.Cold seeps and hydrothermal systems serve...Dissolved carbon(dissolved organic carbon and dissolved inorganic carbon)is the major component of the ocean carbon cycle,representing one of the largest carbon pools on Earth.Cold seeps and hydrothermal systems serve as the two main windows for the material and energy recycling exchange between the lithosphere and outer spheres(biosphere,hydrosphere and atmosphere).However,recent studies have found that the dynamic activities of fluids in these two extreme systems are a crucial source of‘new'carbon in the deep ocean.These carbon sources may become vital contributors to carbon and energy in marine ecosystems,which affect the global deep-sea carbon budget,and the marine ecosystems as well.In this review,we summarize the sources and formation mechanisms of dissolved carbon in the seep fluids from the cold seeps and hydrothermal vents,the contribution of methane oxidation to dissolved carbon,and the characteristics of the carbon isotope composition in the fluid.Furthermore,we analyze and discuss the influence of carbon discharged from seabed on the seawater carbon cycle by comparing and contrasting these two extreme environments.The research may assist in promoting a deeper understanding of the carbon cycle and material interaction in the ocean,particularly further carbon cycle research in the back-arc basin where cold seeps and hydrothermal vents commonly prevail.展开更多
Natural hydrocarbon seeps in the marine environment are important contributors to greenhouse gases in the atmosphere. Such gases include methane, which plays a significant role in global carbon cycling and climate cha...Natural hydrocarbon seeps in the marine environment are important contributors to greenhouse gases in the atmosphere. Such gases include methane, which plays a significant role in global carbon cycling and climate change. To accurately quantify the methane flux from hydrocarbon seeps on the seafloor, a specialized in situ and online gas flux measuring(GFM) device was designed to obtain high-resolution time course gas fluxes using the process of equal volume exchange. The device consists of a 1.0-m diameter, 0.9-m tall, inverted conical tent and a GFM instrument that contains a solenoid valve, level transducer, and gas collection chamber. Rising gas bubbles from seeps were measured by laboratory-calibrated GFM instruments attached to the top of the tent. According to the experimental data, the optimal anti-shake time interval was 5 s. The measurement range of the device was 0–15 L min^(-1), and the relative error was ± 1.0%. The device was initially deployed at an active seep site in the Lingtou Promontory seep field in South China Sea. The amount of gas released from a single gas vent was 30.5 m^3 during the measurement period, and the gas flow rate ranged from 22 to 72 Lh^(-1), depending on tidal period, and was strongly negatively correlated with water depth. The measurement results strongly suggest that oceanic tides and swells had a significant forcing effect on gas flux. Low flow rates were associated with high tides and vice versa. The changes in gas volume escaping from the seafloor seeps could be attributed to the hydrostatic pressure induced by water depth. Our findings suggest that in the marine environment, especially in the shallow shelf area, sea level variation may play an important role in controlling methane release into the ocean. Such releases probably also affect atmospheric methane levels.展开更多
Natural hydrocarbon seeps in a marine environment are one of the important contributors to greenhouse gases in the atmosphere,including methane,which is significant to the global carbon cycling and climate change.Four...Natural hydrocarbon seeps in a marine environment are one of the important contributors to greenhouse gases in the atmosphere,including methane,which is significant to the global carbon cycling and climate change.Four hydrocarbon seep areas,the Lingtou Promontory,the Yinggehai Rivulet mouth,the Yazhou Bay and the Nanshan Promontory,occurring in the Yinggehai Basin delineate a near-shore gas bubble zone.The gas composition and geochemistry of venting bubbles and the spatial distribution of hydrocarbon seeps are surveyed on the near-shore Lingtou Promontory.The gas composition of the venting bubbles is mainly composed of CO_2,CH_4,N_2 and O_2,with minor amounts of non-methane hydrocarbons.The difference in the bubbles＇ composition is a possible consequence of gas exchange during bubble ascent.The seepage gases from the seafloor are characterized by a high CO_2 content（67.35%） and relatively positive δ^（13）C_（V_PDB） values（-0.49×10^（-3）-0.86×10^（-3））,indicating that the CO_2 is of inorganic origin.The relatively low CH_4 content（23%） and their negative δ^（13）C_（V-PDB） values（-34.43×10^（-3）--37.53×10^（-3）） and high ratios of C_1 content to C_（1-5） one（0.98-0.99）as well point to thermogenic gases.The hydrocarbon seeps on the 3.5 Hz sub-bottom profile display a linear arrangement and are sub-parallel to the No.1 fault,suggesting that the hydrocarbon seeps may be associated with fracture activity or weak zones and that the seepage gases migrate laterally from the central depression of the Yinggehai Basin.展开更多
A new methodology was developed to identify cold seeps and structured benthic communities associated, which was applied for the first time in the offshore southern Colombian Caribbean. The integral method consists on ...A new methodology was developed to identify cold seeps and structured benthic communities associated, which was applied for the first time in the offshore southern Colombian Caribbean. The integral method consists on a new processing of double-coverage (200%) high-resolution backscatter data combined with bathymetric information;validation was done correlating identified gas plumes, seabed cores and drift camera surveys. Results showed that the elimination of artefacts and the increased signal of the backscatter data allowed accurate plotting of seep boundaries and categorization of seeps into an activity catalogue, with more than 200 seeps identified. Most seeps have chemosynthetic communities associated and data analysis from a previous survey showed two ridges with hard ground as the only possible areas for the development the of deep- water corals. Seep results were compared with designated Areas of Significance for Biodiversity (ASB) identifying seeps both within and outside the ASBs, and showing that the presence of seeps and chemosynthetic communities associated were driven more by geological processes than for big-scale seabed morphology, since they were found in both plains and ridges. This methodology allows an accurate seabed map of structured benthic communities, which may work as a precise geo-hazard map to ensure the oil & gas industry can avoid these areas of shallow gas for further developments, and as a map of deep-water structured benthic communities with environmental significance.展开更多
For our ancestors, oil seeps were both a fascination and a resource but as the planet's reserves of high quality low density oil becomes increasingly depleted, so there is now a renewed interest in heavier,biodegr...For our ancestors, oil seeps were both a fascination and a resource but as the planet's reserves of high quality low density oil becomes increasingly depleted, so there is now a renewed interest in heavier,biodegraded oils such as those encountered in terrestrial seeps. One such seep is Pitch Lake in the Caribbean island of Trinidad, which is the largest natural deposit of asphalt in the world. At the northern end of the Caribbean, oil emerges along a tectonic contact on the island on Cuba. The sources of the oils from these seeps are relatively recent and both are subject to intense weathering due to the tropical conditions. When analysed by gas chromatography(GC) both oils appear as unresolved complex mixtures(UCM) and show a very high degree of biodegradation thus presenting an analytical challenge. In this case study, these two Caribbean seep oils were analysed by comprehensive two dimensional GC with time of flight mass spectrometry(GC×GC-TOFMS) to expose many thousands of the individual compounds that comprise the UCM. The high chromatographic resolution of the GC×GC-TOFMS produced good quality mass spectra allowing many compounds including molecular fossil ‘biomarkers' to be identified. Compound classes included diamondoid hydrocarbons, demethylated hopanes and secohopanes, mono-and tri-aromatic steroids. D-ring aromatised structures of the 8,14-seco-hopanes,including demethylated forms were present in both oils but further demethylation, probably at position C-25 during biodegradation, was only observed in the Pitch Lake oil. Many polycyclic aromatic hydrocarbons(PAHs) were absent although the fungal-derived pentacyclic PAH perylene was present in both oils. The presence of the angiosperm biomarker lupane in the Pitch Lake oil constrained the age to the Late Cretaceous. The higher degree of biodegradation observed in the Cuban oil was likely due to relatively slow anaerobic processes whereas oil within Pitch Lake was probably subject to additional more rapid aerobic metabolism within the lake.展开更多
To confirm the seabed fluid flow at the Haima cold seeps,an integrated study of multi-beam and seismic data reveals the morphology and fate of four bubble plumes and investigates the detailed subsurface structure of t...To confirm the seabed fluid flow at the Haima cold seeps,an integrated study of multi-beam and seismic data reveals the morphology and fate of four bubble plumes and investigates the detailed subsurface structure of the active seepage area.The shapes of bubble plumes are not constant and influenced by the northeastward bottom currents,but the water depth where these bubble plumes disappear(630–650 m below the sea level)(mbsl)is very close to the upper limit of the gas hydrate stability zone in the water column(620 m below the sea level),as calculated from the CTD data within the study area,supporting the“hydrate skin”hypothesis.Gas chimneys directly below the bottom simulating reflectors,found at most sites,are speculated as essential pathways for both thermogenic gas and biogenic gas migrating from deep formations to the gas hydrate stability zone.The fracture network on the top of the basement uplift may be heavily gas-charged,which accounts for the chimney with several kilometers in diameter(beneath Plumes B and C).The much smaller gas chimney(beneath Plume D)may stem from gas saturated localized strong permeability zone.High-resolution seismic profiles reveal pipe-like structures,characterized by stacked localized amplitude anomalies,just beneath all the plumes,which act as the fluid conduits conveying gas from the gas hydrate-bearing sediments to the seafloor,feeding the gas plumes.The differences between these pipe-like structures indicate the dynamic process of gas seepage,which may be controlled by the build-up and dissipation of pore pressure.The 3D seismic data show high saturated gas hydrates with high RMS amplitude tend to cluster on the periphery of the gas chimney.Understanding the fluid migration and hydrate accumulation pattern of the Haima cold seeps can aid in the further exploration and study on the dynamic gas hydrate system in the South China Sea.展开更多
The succession of benthic fauna characterizes the environmental dynamics of cold seep ecosystems,offering insights into deepsea biodiversity patterns and the influence of geofluid on the local biosphere.Previous studi...The succession of benthic fauna characterizes the environmental dynamics of cold seep ecosystems,offering insights into deepsea biodiversity patterns and the influence of geofluid on the local biosphere.Previous studies documented the presence and abundance of specific taxa at different stages of seep development,revealing the patchy distribution of faunal assemblages[1].As research progresses,specific environmental factors,including the levels of methane,sulfide.展开更多
The paper deals with the urgent problem of gas-geochemical parameters in the seas and shelf transit zones based on a comparison of field studies and remote sensing data.The results of complex gas-geochemical studies o...The paper deals with the urgent problem of gas-geochemical parameters in the seas and shelf transit zones based on a comparison of field studies and remote sensing data.The results of complex gas-geochemical studies of the Daginsky gas-hydrothermal system are presented,as well as an assessment of methane emissions from the studied area.The Daginsky gas-hydrothermal system is located on the northeastern coast of Sakhalin Island,and is a unique object due to a combination of a number of factors:from zonality due to the proximity of the Okhotsk Sea and the geological structure,to the interaction of deep and surface processes manifested in the presence of biogenic and thermogenic methane,as well as mantle helium.Tectonic faults and oil and gas bearing structures of the northeastern shelf of Sakhalin,which determine the direction of natural gas flows and facilitate its migration to the surface,make a significant contribution to the gas appearance of thermal springs.The main gas is methane up to 90%,homologues of methane up to and including pentane have been established,and isolated high concentrations of helium and hydrogen,both dissolved and in the free gas of bubbles,have also been noted.The conducted isotope studies allow to speak about the complex genesis of the gas.δ^(13)C isotopic composition,ranging from−49.4‰to−60.2‰shows the dominance of biogenic methane with an admixture of thermogenic component.This is also confirmed by the presence of a fraction of mantle helium.The flow of methane into the atmosphere from the Daginsky area is 963757.5 mol/(km^(2)·day),or about 15.4 t/(km^(2)·year),which indicates the importance of this region for the regional and global carbon cycle.The dynamics of methane emissions can vary depending on various factors,such as seasonal fluctuations and geological activity,which further complicates the understanding of processes in the region.展开更多
Cold seeps are oases for biological communities on the sea floor around hydrocarbon emission pathways.Microbial utilization of methane and other hydrocarbons yield products that fuel rich chemosynthetic communities at...Cold seeps are oases for biological communities on the sea floor around hydrocarbon emission pathways.Microbial utilization of methane and other hydrocarbons yield products that fuel rich chemosynthetic communities at these sites.One such site in the cold seep ecosystem of Krishna-Godavari basin(K-G basin)along the east coast of India,discovered in Feb 2018 at a depth of 1800 m was assessed for its bacterial diversity.The seep bacterial communities were dominated by phylum Proteobacteria(57%),Firmicutes(16%)and unclassified species belonging to the family Helicobacteriaceae.The surface sediments of the seep had maximum OTUs(operational taxonomic units)(2.27×10^(3))with a Shannon alpha diversity index of 8.06.In general,environmental parameters like total organic carbon(p<0.01),sulfate(p<0.001),sulfide(p<0.05)and methane(p<0.01)were responsible for shaping the bacterial community of the cold seep ecosystem in the K-G Basin.Environmental parameters play a significant role in changing the bacterial diversity richness between different cold seep environments in the oceans.展开更多
This work reports on a preliminary taxonomic study of epibenthic macroinvertebrates collected or observed by underwater video at the Haima cold seeps and in adjacent deep-sea habitats,including a mud volcano feld and ...This work reports on a preliminary taxonomic study of epibenthic macroinvertebrates collected or observed by underwater video at the Haima cold seeps and in adjacent deep-sea habitats,including a mud volcano feld and Ganquan Plateau,during an expedition in the South China Sea by the Chinese-manned submersible Shenhai Yongshi in May 2018.A total of 41 species belonging to 6 phyla were identifed,among which 34 species were collected from the Haima cold seeps.Mollusks and crustaceans that are specialized in reducing habitats were predominant in biotopes of the Haima cold seeps,whereas sponges and cold-water corals and their commensals were prominent in communities of the mud volcano feld and the slopes of Ganquan Plateau.The distribution and faunal composition of each taxonomic group are discussed.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.91858208,92358301)the Laoshan Laboratory(No.LSKJ 202203500),the China Postdoctoral Science Foundation of China(No.2019M663209)the Fundamental Research Funds for the Central Universities in China(No.19lGPY100)。
文摘Bathymodiolus mussels distribute in both deep-sea cold seep and hydrothermal vent environments,whose endosymbiotic gill tissue is a prominent character for the adaptation of extreme habitats.However,few studies explored the adaptation mechanisms through comparative transcriptome sequencing and analysis of different tissues between seep mussels and vent mussels.We performed the comparative transcriptome sequencing and analysis for three tissue types(gill,mantle,and adductor muscle)of Bathymodiolus mussels collected from a cold seeping site Station S 11 and the 50-km away hydrothermal field Minami-Ensei Knoll in the Okinawa Trough.Results show that gene expression patterns had distinct tissue specificity.Compared with the non-endosymbiotic tissues(mantle and adductor muscle),the significantly strengthened gene functions in endosymbiotic gill included microbial recognition(fibrinogen C domain-containing protein 1-B-like(fibcd),fibrinogen-related protein 8(frp),peptidoglycan recognition proteins(pgrp),and C-type lectin(clec)),cell apoptosis and immunity(interferon regulatory factor 1/2-like 1(ir f),cathepsin D(ctsd),caspase 2(casp 2)),and antioxidant capacity(copper/zinc superoxide dismutase(czsod),glutathione peroxidase(gpx),selenoprotein(sel)),in both seep and vent individuals.Consistent with metal accumulation,high expression levels of genes related to heavy metal detoxification(cytochrome P 450(cyp),ferritin-like(ftl),metallothionein(mt),glutathione S-transferase(gst))were also observed in gill.Moreover,to adapt to high hydrostatic pressure in the deep sea,the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways associated with cellular community were significantly enriched in all three tissues,suggesting the regulation of cell structure and cell adhesion at transcriptional level.This study obtained gene expression profiles of deep-sea mussels subsisting at cold seep and hydrothermal vent sites,which could lay foundations for comprehensive investigations of molecular basis in adaptation of deep-sea mussels to the two extreme ecosystems.
基金supported by the Shandong Provincial Natural Science Foundation(No.ZR2021MD049)the National Natural Science Foundation of China(Nos.42176057,and 92358301)the Marine Geological Survey Program(Nos.DD20230402)。
文摘Dissolved carbon(dissolved organic carbon and dissolved inorganic carbon)is the major component of the ocean carbon cycle,representing one of the largest carbon pools on Earth.Cold seeps and hydrothermal systems serve as the two main windows for the material and energy recycling exchange between the lithosphere and outer spheres(biosphere,hydrosphere and atmosphere).However,recent studies have found that the dynamic activities of fluids in these two extreme systems are a crucial source of‘new'carbon in the deep ocean.These carbon sources may become vital contributors to carbon and energy in marine ecosystems,which affect the global deep-sea carbon budget,and the marine ecosystems as well.In this review,we summarize the sources and formation mechanisms of dissolved carbon in the seep fluids from the cold seeps and hydrothermal vents,the contribution of methane oxidation to dissolved carbon,and the characteristics of the carbon isotope composition in the fluid.Furthermore,we analyze and discuss the influence of carbon discharged from seabed on the seawater carbon cycle by comparing and contrasting these two extreme environments.The research may assist in promoting a deeper understanding of the carbon cycle and material interaction in the ocean,particularly further carbon cycle research in the back-arc basin where cold seeps and hydrothermal vents commonly prevail.
基金provided by the National Scientific Foundation of China (Nos. 41676046 and 41306045)the Knowledge Innovation Project of the Chinese Academy of Sciences (Nos. SIDSSE-201208 and SQ201110)+1 种基金the 'Hundred Talents Program' of the Chinese Academy of Sciencesfinancial support from China Scholarship Council
文摘Natural hydrocarbon seeps in the marine environment are important contributors to greenhouse gases in the atmosphere. Such gases include methane, which plays a significant role in global carbon cycling and climate change. To accurately quantify the methane flux from hydrocarbon seeps on the seafloor, a specialized in situ and online gas flux measuring(GFM) device was designed to obtain high-resolution time course gas fluxes using the process of equal volume exchange. The device consists of a 1.0-m diameter, 0.9-m tall, inverted conical tent and a GFM instrument that contains a solenoid valve, level transducer, and gas collection chamber. Rising gas bubbles from seeps were measured by laboratory-calibrated GFM instruments attached to the top of the tent. According to the experimental data, the optimal anti-shake time interval was 5 s. The measurement range of the device was 0–15 L min^(-1), and the relative error was ± 1.0%. The device was initially deployed at an active seep site in the Lingtou Promontory seep field in South China Sea. The amount of gas released from a single gas vent was 30.5 m^3 during the measurement period, and the gas flow rate ranged from 22 to 72 Lh^(-1), depending on tidal period, and was strongly negatively correlated with water depth. The measurement results strongly suggest that oceanic tides and swells had a significant forcing effect on gas flux. Low flow rates were associated with high tides and vice versa. The changes in gas volume escaping from the seafloor seeps could be attributed to the hydrostatic pressure induced by water depth. Our findings suggest that in the marine environment, especially in the shallow shelf area, sea level variation may play an important role in controlling methane release into the ocean. Such releases probably also affect atmospheric methane levels.
基金The National Natural Science Foundation of China under contract Nos 41306045,91228206,41422602 and 41676046the Hundred Talents Program of the Chinese Academy of Sciences
文摘Natural hydrocarbon seeps in a marine environment are one of the important contributors to greenhouse gases in the atmosphere,including methane,which is significant to the global carbon cycling and climate change.Four hydrocarbon seep areas,the Lingtou Promontory,the Yinggehai Rivulet mouth,the Yazhou Bay and the Nanshan Promontory,occurring in the Yinggehai Basin delineate a near-shore gas bubble zone.The gas composition and geochemistry of venting bubbles and the spatial distribution of hydrocarbon seeps are surveyed on the near-shore Lingtou Promontory.The gas composition of the venting bubbles is mainly composed of CO_2,CH_4,N_2 and O_2,with minor amounts of non-methane hydrocarbons.The difference in the bubbles＇ composition is a possible consequence of gas exchange during bubble ascent.The seepage gases from the seafloor are characterized by a high CO_2 content（67.35%） and relatively positive δ^（13）C_（V_PDB） values（-0.49×10^（-3）-0.86×10^（-3））,indicating that the CO_2 is of inorganic origin.The relatively low CH_4 content（23%） and their negative δ^（13）C_（V-PDB） values（-34.43×10^（-3）--37.53×10^（-3）） and high ratios of C_1 content to C_（1-5） one（0.98-0.99）as well point to thermogenic gases.The hydrocarbon seeps on the 3.5 Hz sub-bottom profile display a linear arrangement and are sub-parallel to the No.1 fault,suggesting that the hydrocarbon seeps may be associated with fracture activity or weak zones and that the seepage gases migrate laterally from the central depression of the Yinggehai Basin.
文摘A new methodology was developed to identify cold seeps and structured benthic communities associated, which was applied for the first time in the offshore southern Colombian Caribbean. The integral method consists on a new processing of double-coverage (200%) high-resolution backscatter data combined with bathymetric information;validation was done correlating identified gas plumes, seabed cores and drift camera surveys. Results showed that the elimination of artefacts and the increased signal of the backscatter data allowed accurate plotting of seep boundaries and categorization of seeps into an activity catalogue, with more than 200 seeps identified. Most seeps have chemosynthetic communities associated and data analysis from a previous survey showed two ridges with hard ground as the only possible areas for the development the of deep- water corals. Seep results were compared with designated Areas of Significance for Biodiversity (ASB) identifying seeps both within and outside the ASBs, and showing that the presence of seeps and chemosynthetic communities associated were driven more by geological processes than for big-scale seabed morphology, since they were found in both plains and ridges. This methodology allows an accurate seabed map of structured benthic communities, which may work as a precise geo-hazard map to ensure the oil & gas industry can avoid these areas of shallow gas for further developments, and as a map of deep-water structured benthic communities with environmental significance.
文摘For our ancestors, oil seeps were both a fascination and a resource but as the planet's reserves of high quality low density oil becomes increasingly depleted, so there is now a renewed interest in heavier,biodegraded oils such as those encountered in terrestrial seeps. One such seep is Pitch Lake in the Caribbean island of Trinidad, which is the largest natural deposit of asphalt in the world. At the northern end of the Caribbean, oil emerges along a tectonic contact on the island on Cuba. The sources of the oils from these seeps are relatively recent and both are subject to intense weathering due to the tropical conditions. When analysed by gas chromatography(GC) both oils appear as unresolved complex mixtures(UCM) and show a very high degree of biodegradation thus presenting an analytical challenge. In this case study, these two Caribbean seep oils were analysed by comprehensive two dimensional GC with time of flight mass spectrometry(GC×GC-TOFMS) to expose many thousands of the individual compounds that comprise the UCM. The high chromatographic resolution of the GC×GC-TOFMS produced good quality mass spectra allowing many compounds including molecular fossil ‘biomarkers' to be identified. Compound classes included diamondoid hydrocarbons, demethylated hopanes and secohopanes, mono-and tri-aromatic steroids. D-ring aromatised structures of the 8,14-seco-hopanes,including demethylated forms were present in both oils but further demethylation, probably at position C-25 during biodegradation, was only observed in the Pitch Lake oil. Many polycyclic aromatic hydrocarbons(PAHs) were absent although the fungal-derived pentacyclic PAH perylene was present in both oils. The presence of the angiosperm biomarker lupane in the Pitch Lake oil constrained the age to the Late Cretaceous. The higher degree of biodegradation observed in the Cuban oil was likely due to relatively slow anaerobic processes whereas oil within Pitch Lake was probably subject to additional more rapid aerobic metabolism within the lake.
基金The Shandong Province “Taishan Scholar” Construction Projectthe fund of the Laboratory for Marine Mineral Resources,Pilot National Laboratory for Marine Science and Technology (Qingdao) under contract No.MMRKF201810+1 种基金the National Natural Science Foundation of China under contract No.41606077the National Key R&D Program of China under contract No.2018YFC0310000.
文摘To confirm the seabed fluid flow at the Haima cold seeps,an integrated study of multi-beam and seismic data reveals the morphology and fate of four bubble plumes and investigates the detailed subsurface structure of the active seepage area.The shapes of bubble plumes are not constant and influenced by the northeastward bottom currents,but the water depth where these bubble plumes disappear(630–650 m below the sea level)(mbsl)is very close to the upper limit of the gas hydrate stability zone in the water column(620 m below the sea level),as calculated from the CTD data within the study area,supporting the“hydrate skin”hypothesis.Gas chimneys directly below the bottom simulating reflectors,found at most sites,are speculated as essential pathways for both thermogenic gas and biogenic gas migrating from deep formations to the gas hydrate stability zone.The fracture network on the top of the basement uplift may be heavily gas-charged,which accounts for the chimney with several kilometers in diameter(beneath Plumes B and C).The much smaller gas chimney(beneath Plume D)may stem from gas saturated localized strong permeability zone.High-resolution seismic profiles reveal pipe-like structures,characterized by stacked localized amplitude anomalies,just beneath all the plumes,which act as the fluid conduits conveying gas from the gas hydrate-bearing sediments to the seafloor,feeding the gas plumes.The differences between these pipe-like structures indicate the dynamic process of gas seepage,which may be controlled by the build-up and dissipation of pore pressure.The 3D seismic data show high saturated gas hydrates with high RMS amplitude tend to cluster on the periphery of the gas chimney.Understanding the fluid migration and hydrate accumulation pattern of the Haima cold seeps can aid in the further exploration and study on the dynamic gas hydrate system in the South China Sea.
基金supported by the National Natural Science Founda-tion of China(42122045 and 42306171)the Development Fund of South China Sea Institute of Oceanology,Chinese Academy of Sciences(SCSIO202202)the PI project of Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)(GML20190609).
文摘The succession of benthic fauna characterizes the environmental dynamics of cold seep ecosystems,offering insights into deepsea biodiversity patterns and the influence of geofluid on the local biosphere.Previous studies documented the presence and abundance of specific taxa at different stages of seep development,revealing the patchy distribution of faunal assemblages[1].As research progresses,specific environmental factors,including the levels of methane,sulfide.
基金funded by a grant Russian Science Foundation(No.23-77-10038,https://rscf.ru/project/23-77-10038/)partly within the framework of the state task of the POI FEB RAS(No.124022100078-7).
文摘The paper deals with the urgent problem of gas-geochemical parameters in the seas and shelf transit zones based on a comparison of field studies and remote sensing data.The results of complex gas-geochemical studies of the Daginsky gas-hydrothermal system are presented,as well as an assessment of methane emissions from the studied area.The Daginsky gas-hydrothermal system is located on the northeastern coast of Sakhalin Island,and is a unique object due to a combination of a number of factors:from zonality due to the proximity of the Okhotsk Sea and the geological structure,to the interaction of deep and surface processes manifested in the presence of biogenic and thermogenic methane,as well as mantle helium.Tectonic faults and oil and gas bearing structures of the northeastern shelf of Sakhalin,which determine the direction of natural gas flows and facilitate its migration to the surface,make a significant contribution to the gas appearance of thermal springs.The main gas is methane up to 90%,homologues of methane up to and including pentane have been established,and isolated high concentrations of helium and hydrogen,both dissolved and in the free gas of bubbles,have also been noted.The conducted isotope studies allow to speak about the complex genesis of the gas.δ^(13)C isotopic composition,ranging from−49.4‰to−60.2‰shows the dominance of biogenic methane with an admixture of thermogenic component.This is also confirmed by the presence of a fraction of mantle helium.The flow of methane into the atmosphere from the Daginsky area is 963757.5 mol/(km^(2)·day),or about 15.4 t/(km^(2)·year),which indicates the importance of this region for the regional and global carbon cycle.The dynamics of methane emissions can vary depending on various factors,such as seasonal fluctuations and geological activity,which further complicates the understanding of processes in the region.
文摘Cold seeps are oases for biological communities on the sea floor around hydrocarbon emission pathways.Microbial utilization of methane and other hydrocarbons yield products that fuel rich chemosynthetic communities at these sites.One such site in the cold seep ecosystem of Krishna-Godavari basin(K-G basin)along the east coast of India,discovered in Feb 2018 at a depth of 1800 m was assessed for its bacterial diversity.The seep bacterial communities were dominated by phylum Proteobacteria(57%),Firmicutes(16%)and unclassified species belonging to the family Helicobacteriaceae.The surface sediments of the seep had maximum OTUs(operational taxonomic units)(2.27×10^(3))with a Shannon alpha diversity index of 8.06.In general,environmental parameters like total organic carbon(p<0.01),sulfate(p<0.001),sulfide(p<0.05)and methane(p<0.01)were responsible for shaping the bacterial community of the cold seep ecosystem in the K-G Basin.Environmental parameters play a significant role in changing the bacterial diversity richness between different cold seep environments in the oceans.
基金This work was financially supported by the National Key R&D Program of China,(No.2018YFC0310800)the China Ocean Mineral Resources Research and Development Association Program(Nos.DY135-E2-3-04&DY135-E2-1-02)+1 种基金the National Natural Science Foundation of China(NSFC)(Nos.41876178&31572229)the Senior User Project of RV KEXUE(No.KEXUE2018G25).
文摘This work reports on a preliminary taxonomic study of epibenthic macroinvertebrates collected or observed by underwater video at the Haima cold seeps and in adjacent deep-sea habitats,including a mud volcano feld and Ganquan Plateau,during an expedition in the South China Sea by the Chinese-manned submersible Shenhai Yongshi in May 2018.A total of 41 species belonging to 6 phyla were identifed,among which 34 species were collected from the Haima cold seeps.Mollusks and crustaceans that are specialized in reducing habitats were predominant in biotopes of the Haima cold seeps,whereas sponges and cold-water corals and their commensals were prominent in communities of the mud volcano feld and the slopes of Ganquan Plateau.The distribution and faunal composition of each taxonomic group are discussed.
