Liquid-liquid phase separation(LLPS)of proteins and nucleic acids is a common phenomenon in cells that underlies the formation of membraneless organelles.Although the macroscopic behavior of biomolecular coacervates h...Liquid-liquid phase separation(LLPS)of proteins and nucleic acids is a common phenomenon in cells that underlies the formation of membraneless organelles.Although the macroscopic behavior of biomolecular coacervates has been elucidated by microscopy,the detailed dynamic properties of proteins/peptides during the LLPS process remain poorly characterized.Here,site-directed spin labeling-electron paramagnetic resonance(SDSL-EPR)spectroscopy was employed to characterize the dynamic properties of a minimal model LLPS system consisting of positively charged peptides and RNA.The degree of phase separation,indicated by broadening of the EPR spectrum of the spin-labeled peptide due to slow molecular tumbling,was monitored by EPR.In addition,three distinct populations with varying molecular motion during LLPS,featuring different spectral lineshapes,were identified.These populations included a fast motion component(Ⅰ),a slower motion component(Ⅱ)associated with peptides in the dispersed phase and an immobile component(Ⅲ)observed in the dense phase.With gradual titration of the peptides to RNA,the EPR spectrum gradually shifted,refiecting changes in the populations of the components.Together,SDSL-EPR method not only provides new insights into the dynamic behavior of biomolecules during LLPS,but also offers a sensitive method for biomolecular phase separation processes at the molecular level.展开更多
随着近年来我国自然灾害与矿山安全事故的频繁发生,由其造成的人员伤亡和经济损失也妨碍了我国的发展与进步。尤其是矿山安全事故,其复杂性和险恶性,在一定程度上也阻碍了救援人员的指挥工作。如何快速、准确的实施救援行动,是我国亟待...随着近年来我国自然灾害与矿山安全事故的频繁发生,由其造成的人员伤亡和经济损失也妨碍了我国的发展与进步。尤其是矿山安全事故,其复杂性和险恶性,在一定程度上也阻碍了救援人员的指挥工作。如何快速、准确的实施救援行动,是我国亟待解决的一个重要难题。在煤矿中运用对称性数字用户环路(Symmetric Digital Subscriber Line.SDSL多媒体技术,旨在提高我国矿山事故的救援水平。展开更多
基金supported by the National Natural Science Foundation of China(No.21927814)the National Key Research and Development Program of China(Nos.2019YFA0405600,2019YFA0706900,2021YFA1200104,2022YFC3400500)+2 种基金the Strategic Priority Research Program of Chinese Academy of Sciences(Nos.XDB0540200,XDB37040201)Plans for Major Provincial Science&Technology Projects(No.202303a07020004)the Youth Innovation Promotion Association,CAS(No.2022455)。
文摘Liquid-liquid phase separation(LLPS)of proteins and nucleic acids is a common phenomenon in cells that underlies the formation of membraneless organelles.Although the macroscopic behavior of biomolecular coacervates has been elucidated by microscopy,the detailed dynamic properties of proteins/peptides during the LLPS process remain poorly characterized.Here,site-directed spin labeling-electron paramagnetic resonance(SDSL-EPR)spectroscopy was employed to characterize the dynamic properties of a minimal model LLPS system consisting of positively charged peptides and RNA.The degree of phase separation,indicated by broadening of the EPR spectrum of the spin-labeled peptide due to slow molecular tumbling,was monitored by EPR.In addition,three distinct populations with varying molecular motion during LLPS,featuring different spectral lineshapes,were identified.These populations included a fast motion component(Ⅰ),a slower motion component(Ⅱ)associated with peptides in the dispersed phase and an immobile component(Ⅲ)observed in the dense phase.With gradual titration of the peptides to RNA,the EPR spectrum gradually shifted,refiecting changes in the populations of the components.Together,SDSL-EPR method not only provides new insights into the dynamic behavior of biomolecules during LLPS,but also offers a sensitive method for biomolecular phase separation processes at the molecular level.
文摘矿山救援应急多媒体通信同时兼有时间的突发性、地点的不确定性、地形的复杂性和信息的交互性等特点,对信息的传输和交换都提出了更高的要求。系统采用对称数字用户线点对点宽带连接技术,利用一对电话线进行双向对称数字信号传输,0.5 mm线径的电话线在2 km的距离内可以达到1.5 M b/s的速率,提供了一种可实时监视和直接联络井下事故现场的技术手段。
文摘随着近年来我国自然灾害与矿山安全事故的频繁发生,由其造成的人员伤亡和经济损失也妨碍了我国的发展与进步。尤其是矿山安全事故,其复杂性和险恶性,在一定程度上也阻碍了救援人员的指挥工作。如何快速、准确的实施救援行动,是我国亟待解决的一个重要难题。在煤矿中运用对称性数字用户环路(Symmetric Digital Subscriber Line.SDSL多媒体技术,旨在提高我国矿山事故的救援水平。
