为探讨超级杂交中稻超高产栽培的缓释尿素(硫包衣尿素,SCU)最佳施用量及其环境效应,在大田试验条件下,研究了SCU不同用量对超级杂交中稻Y两优1号产量以及对土水中氮素含量的影响。结果表明,与普通尿素相比,施用SCU有显著的增产效果,同...为探讨超级杂交中稻超高产栽培的缓释尿素(硫包衣尿素,SCU)最佳施用量及其环境效应,在大田试验条件下,研究了SCU不同用量对超级杂交中稻Y两优1号产量以及对土水中氮素含量的影响。结果表明,与普通尿素相比,施用SCU有显著的增产效果,同等施氮量(180 kg N.hm-2)的SCU和普通尿素相比,增产幅度为13.2%;施用135 kg N.hm-2的SCU与180 kg N.hm-2普通尿素相比(氮素用量减少25%)仍可增产12.9%。稻田施氮量可显著影响土壤中铵态氮的累积和分布,但对硝态氮的影响较小。折合等量纯氮的SCU与普通尿素处理相比,可以显著减少肥料施用初期土壤水中氮素含量,施肥后10 d,SCU处理农田面水、0～20 cm层和20～40 cm层土壤水全氮含量分别为施用普通尿素处理的26.3%、64.4%和64.0%。展开更多
Strain Pseudomonas Aeraginosa SCU isolated from rotten hides is shown to produce various gelatinolytic enzymes with molecular masses ranging from ～50 to ～200 kD. A gelatinolytic enzyme called PAC exhibiting collagen...Strain Pseudomonas Aeraginosa SCU isolated from rotten hides is shown to produce various gelatinolytic enzymes with molecular masses ranging from ～50 to ～200 kD. A gelatinolytic enzyme called PAC exhibiting collagenolytic activity is purified by SP sepharose fast flow, Sephadex G-200 gel filtration and native PAGE cutting method. The purified enzyme has an apparent molecular weight of about 110 kD by SDS PAGE without β-mercaptoethanol. Treatment withβ-Me suggests that PAC is dissociated into three subunits approximately 33 kD,25 kD and 20 kD with a ratio of 2∶1∶1, named sub A, sub B and sub C repectively. EDTA and EGTA display a significant inhibitory effect on the enzyme activity while PMSF, leupeptin and pepstain do not appreciably inhibit it. The first 15 amino acid residues of the major subunit (subA) are determined and the sequence is Ala-Glu-Ala-Gly-Gly-Pro-Gly-Gly-Asn-Gln-Lys-Ile-Gly -Lys-Tyr. This sequence is identical to that of elastase of P.aeruginosa. The fragment of encoding mature sub A is cloned and its sequence is determined, which has a high homology with the gene of elastase. These results indicate that PAC is a novel collagenolytic metalloprotease composed of three kinds of subunits, of which elastase is the major one.展开更多
文摘为探讨超级杂交中稻超高产栽培的缓释尿素(硫包衣尿素,SCU)最佳施用量及其环境效应,在大田试验条件下,研究了SCU不同用量对超级杂交中稻Y两优1号产量以及对土水中氮素含量的影响。结果表明,与普通尿素相比,施用SCU有显著的增产效果,同等施氮量(180 kg N.hm-2)的SCU和普通尿素相比,增产幅度为13.2%;施用135 kg N.hm-2的SCU与180 kg N.hm-2普通尿素相比(氮素用量减少25%)仍可增产12.9%。稻田施氮量可显著影响土壤中铵态氮的累积和分布,但对硝态氮的影响较小。折合等量纯氮的SCU与普通尿素处理相比,可以显著减少肥料施用初期土壤水中氮素含量,施肥后10 d,SCU处理农田面水、0～20 cm层和20～40 cm层土壤水全氮含量分别为施用普通尿素处理的26.3%、64.4%和64.0%。
文摘Strain Pseudomonas Aeraginosa SCU isolated from rotten hides is shown to produce various gelatinolytic enzymes with molecular masses ranging from ～50 to ～200 kD. A gelatinolytic enzyme called PAC exhibiting collagenolytic activity is purified by SP sepharose fast flow, Sephadex G-200 gel filtration and native PAGE cutting method. The purified enzyme has an apparent molecular weight of about 110 kD by SDS PAGE without β-mercaptoethanol. Treatment withβ-Me suggests that PAC is dissociated into three subunits approximately 33 kD,25 kD and 20 kD with a ratio of 2∶1∶1, named sub A, sub B and sub C repectively. EDTA and EGTA display a significant inhibitory effect on the enzyme activity while PMSF, leupeptin and pepstain do not appreciably inhibit it. The first 15 amino acid residues of the major subunit (subA) are determined and the sequence is Ala-Glu-Ala-Gly-Gly-Pro-Gly-Gly-Asn-Gln-Lys-Ile-Gly -Lys-Tyr. This sequence is identical to that of elastase of P.aeruginosa. The fragment of encoding mature sub A is cloned and its sequence is determined, which has a high homology with the gene of elastase. These results indicate that PAC is a novel collagenolytic metalloprotease composed of three kinds of subunits, of which elastase is the major one.
