A novel photosensitive copolymer P（SS-co-AA-g-GMA）（PSAG） was synthesized and utilized to noncovalently functionalize pristine single-walled carbon nanotubes（SCNTs）. PSAG was highly effective for the solubilizati...A novel photosensitive copolymer P（SS-co-AA-g-GMA）（PSAG） was synthesized and utilized to noncovalently functionalize pristine single-walled carbon nanotubes（SCNTs）. PSAG was highly effective for the solubilization of SCNTs in water and validated by UV-vis absorption spectra experiments, resulting in homogeneous and stable PSAG-SCNT aqueous dispersion. The microstructure of SCNTs was observed through Raman spectroscopy and transmission electron microscopy. In addition, compared with the two common polymeric dispersants of Triton X-100 and PSS, PSAG demonstrated more effective performances for dispersing SCNTs under identical conditions. Furthermore, the photosensitive PSAG-SCNTs can be crosslinked after UV irradiation, leading to significant improvement in the water resistance of SCNT films. UV-cured films can be transferred to plastic wrap to form a flexible film with high electrical conductivity.展开更多
Successful cloning through somatic cell nuclear transfer(SCNT)faces significant challenges due to epigenetic obstacles.Recent studies have highlighted the roles of H3K4me3 and H3K27me3 as potential contributors to the...Successful cloning through somatic cell nuclear transfer(SCNT)faces significant challenges due to epigenetic obstacles.Recent studies have highlighted the roles of H3K4me3 and H3K27me3 as potential contributors to these obstacles.However,the underlying mechanisms remain largely unclear.In this study,we generated genome-wide maps of H3K4me3 and H3K27me3 in mouse pre-implantation NT embryos.Our analysis revealed that aberrantly over-represented broad H3K4me3 domain and H3K27me3 signal lead to increased bivalent marks at gene promoters in NT embryos compared with naturally fertilized(NF)embryos at the 2-cell stage,which may link to relatively low levels of H3K36me3 in NT 2-cell embryos.Notably,the overexpression of Setd2,a H3K36me3 methyltransferase,successfully restored multiple epigenetic marks,including H3K36me3,H3K4me3,and H3K27me3.In addition,it reinstated the expression levels of ZGA-related genes by reestablishing H3K36me3 at gene body regions,which excluded H3K27me3 from bivalent promoters,ultimately improving cloning efficiency.These findings highlight the excessive bivalent state at gene promoters as a potent barrier and emphasize the removal of these barriers as a promising approach for achieving higher cloning efficiency.展开更多
本研究的目的是探索自青春期前奶山羊获取大量可用于体细胞核移植的卵母细胞的可能性。为此,本研究比较了几种不同组合的激素处理方法(对照、FSH、E2-P4和E2-P4-FSH)对出生39-60日龄的奶山羊卵巢大小、卵泡数量和卵泡大小的影响:同时将...本研究的目的是探索自青春期前奶山羊获取大量可用于体细胞核移植的卵母细胞的可能性。为此,本研究比较了几种不同组合的激素处理方法(对照、FSH、E2-P4和E2-P4-FSH)对出生39-60日龄的奶山羊卵巢大小、卵泡数量和卵泡大小的影响:同时将出生39-120日龄奶山羊按年龄分成三组来研究年龄对激素处理时招募起始生长卵泡数量的影响:然后,比较了来自E2-P4- FSH和FSH处理的早青春期前奶山羊卵巢上直径大于3mm卵泡中卵母细胞减数分裂能力;最后,通过SCNT方法验证E2-P4-FSH处理的早青春期前奶山羊卵巢上直径大于3mm卵泡中卵母细胞的发育能力。在四组激素处理的早青春期前奶山羊中,E2-P4-FSH处理组的卵巢最大、卵泡(直径大于3 mm)数量最多。在不同的年龄组中,39-60天组奶山羊卵巢上直径大于3mm的卵泡数量显著多于61-90天和91-120天组的。卵母细胞减数分裂能力的分析结果表明,来自E2-P4-FSH处理组的卵母细胞减数分裂能力显著高于FSH处理组的卵母细胞。与E2-P4-FSH处理后的成年奶山羊卵母细胞相比,早青春期前奶山羊卵母细胞发育能力较低:卵母细胞成熟后,作为受体用于体细胞核移植后的克隆囊胚发育率低于成年奶山羊(15.3%versus 22.1%,P<0.01)。然而,早青春期前的奶山羊经E2-P4-FSH处理后,自每头羊卵巢上直径大于3mm的卵泡数显著高于成年奶山羊(108±10.3 versus 28±5.0),因此,每头早青春期前奶山羊产生的克隆囊胚绝对数量显著高于成年奶山羊(7.1±2.7 versus 4.2±1.4)。由此,从本研究可以得出结论:E2-P4-FSH处理的早青春期前奶山羊能够为体细胞核移植研究提供相对多数量的具备一定发育能力的成熟卵。展开更多
基金Funded by the National Natural Science Foundation of China(No.51403082)
文摘A novel photosensitive copolymer P（SS-co-AA-g-GMA）（PSAG） was synthesized and utilized to noncovalently functionalize pristine single-walled carbon nanotubes（SCNTs）. PSAG was highly effective for the solubilization of SCNTs in water and validated by UV-vis absorption spectra experiments, resulting in homogeneous and stable PSAG-SCNT aqueous dispersion. The microstructure of SCNTs was observed through Raman spectroscopy and transmission electron microscopy. In addition, compared with the two common polymeric dispersants of Triton X-100 and PSS, PSAG demonstrated more effective performances for dispersing SCNTs under identical conditions. Furthermore, the photosensitive PSAG-SCNTs can be crosslinked after UV irradiation, leading to significant improvement in the water resistance of SCNT films. UV-cured films can be transferred to plastic wrap to form a flexible film with high electrical conductivity.
基金primarily funded by the National Key R&D Program of China(Grant Nos.2022YFA1106000 to X.L.,2022YFC2702200 to S.G.and C.L.,2021YFA1102900 to X.L.)the National Natural Science Foundation of China(Grant Nos.32470857 to C.L.,32370868 and 32070802 to X.L.,32330030 to S.G.,32400678 to R.X.)+4 种基金supported by the Science and Technology Commission of Shanghai Municipality(Grant Nos.23JC1403700 to S.G.)the National Postdoctoral Program for Innovative Talents(Grant Nos.BX20240264 to R.X.)the China Postdoctoral Science Foundation(Grant Nos.2023M742658 to R.X.)the Shanghai Municipal Science and Technology Major Project to S.G.the Fundamental Research Funds for the Central Universities to S.G.and X.L.and Peak Disciplines(Type IV)of Institutions of Higher Learning in Shanghai to S.G.
文摘Successful cloning through somatic cell nuclear transfer(SCNT)faces significant challenges due to epigenetic obstacles.Recent studies have highlighted the roles of H3K4me3 and H3K27me3 as potential contributors to these obstacles.However,the underlying mechanisms remain largely unclear.In this study,we generated genome-wide maps of H3K4me3 and H3K27me3 in mouse pre-implantation NT embryos.Our analysis revealed that aberrantly over-represented broad H3K4me3 domain and H3K27me3 signal lead to increased bivalent marks at gene promoters in NT embryos compared with naturally fertilized(NF)embryos at the 2-cell stage,which may link to relatively low levels of H3K36me3 in NT 2-cell embryos.Notably,the overexpression of Setd2,a H3K36me3 methyltransferase,successfully restored multiple epigenetic marks,including H3K36me3,H3K4me3,and H3K27me3.In addition,it reinstated the expression levels of ZGA-related genes by reestablishing H3K36me3 at gene body regions,which excluded H3K27me3 from bivalent promoters,ultimately improving cloning efficiency.These findings highlight the excessive bivalent state at gene promoters as a potent barrier and emphasize the removal of these barriers as a promising approach for achieving higher cloning efficiency.
文摘本研究的目的是探索自青春期前奶山羊获取大量可用于体细胞核移植的卵母细胞的可能性。为此,本研究比较了几种不同组合的激素处理方法(对照、FSH、E2-P4和E2-P4-FSH)对出生39-60日龄的奶山羊卵巢大小、卵泡数量和卵泡大小的影响:同时将出生39-120日龄奶山羊按年龄分成三组来研究年龄对激素处理时招募起始生长卵泡数量的影响:然后,比较了来自E2-P4- FSH和FSH处理的早青春期前奶山羊卵巢上直径大于3mm卵泡中卵母细胞减数分裂能力;最后,通过SCNT方法验证E2-P4-FSH处理的早青春期前奶山羊卵巢上直径大于3mm卵泡中卵母细胞的发育能力。在四组激素处理的早青春期前奶山羊中,E2-P4-FSH处理组的卵巢最大、卵泡(直径大于3 mm)数量最多。在不同的年龄组中,39-60天组奶山羊卵巢上直径大于3mm的卵泡数量显著多于61-90天和91-120天组的。卵母细胞减数分裂能力的分析结果表明,来自E2-P4-FSH处理组的卵母细胞减数分裂能力显著高于FSH处理组的卵母细胞。与E2-P4-FSH处理后的成年奶山羊卵母细胞相比,早青春期前奶山羊卵母细胞发育能力较低:卵母细胞成熟后,作为受体用于体细胞核移植后的克隆囊胚发育率低于成年奶山羊(15.3%versus 22.1%,P<0.01)。然而,早青春期前的奶山羊经E2-P4-FSH处理后,自每头羊卵巢上直径大于3mm的卵泡数显著高于成年奶山羊(108±10.3 versus 28±5.0),因此,每头早青春期前奶山羊产生的克隆囊胚绝对数量显著高于成年奶山羊(7.1±2.7 versus 4.2±1.4)。由此,从本研究可以得出结论:E2-P4-FSH处理的早青春期前奶山羊能够为体细胞核移植研究提供相对多数量的具备一定发育能力的成熟卵。
