Ubiquitination,a critical post-translational modification,plays a pivotal role in fine tuning the immune responses of plants.The tomato(Solanum lycopersicum)suffers significant yield and quality losses caused by the d...Ubiquitination,a critical post-translational modification,plays a pivotal role in fine tuning the immune responses of plants.The tomato(Solanum lycopersicum)suffers significant yield and quality losses caused by the devastating pathogen Botrytis cinerea.We have discovered the role of SlRAE1,a gene encoding an E3 ubiquitin ligase,as a pivotal negative regulator of resistance to B.cinerea.SlRAE1 interacts with SlSKP1,a component of the SKP1–Cullin1–F-box(SCF)complex,to modulate the protein stability of the transcription factor SlWRKY1 through the 26S proteasome pathway.SlWRKY1 targets and inhibits the transcription of SlJAZ7,a suppressor of jasmonic acid(JA)signaling,thereby activating the JA-induced defense system and affecting tomato susceptibility to B.cinerea.The resistance enhancement observed with knock-out SlRAE1 was reduced when SlWRKY1 was also knocked out,highlighting SlWRKY1's role in SlRAE1's regulation of tomato defense against B.cinerea.Our findings elucidate the defense mechanism in tomato and suggest that targeting SlRAE1,by modulating SlWRKY1 stability,could help to develop resistant tomato varieties.These insights have broader implications for using gene-editing technologies to enhance crop defense against fungi.展开更多
功能性消化不良(FD)的胃肠动力障碍与Cajal间质细胞(ICC)网络损伤密切相关。文章系统阐述干细胞因子(SCF)/c-kit信号通路在FD发病中的核心作用,提出柴桂和胃方通过三重机制修复ICC网络:1) 柴胡皂苷激活c-kit受体促ICC增殖;2) 桂枝成分...功能性消化不良(FD)的胃肠动力障碍与Cajal间质细胞(ICC)网络损伤密切相关。文章系统阐述干细胞因子(SCF)/c-kit信号通路在FD发病中的核心作用,提出柴桂和胃方通过三重机制修复ICC网络:1) 柴胡皂苷激活c-kit受体促ICC增殖;2) 桂枝成分抑制肿瘤坏死因子-α (TNF-α)改善炎症微环境;3) 白芍苷调节5-羟色胺(5-HT)信号恢复脑肠轴平衡。基于“肝郁脾虚-ICC功能障碍”病机对应理论,构建中药复方多靶点修复胃肠起搏网络的新假说,为中西医结合治疗提供理论依据。Gastrointestinal dyskinesia in functional dyspepsia (FD) is closely related to the damage of Cajal interstitial cell (ICC) network. In this paper, the central role of the stem cell factor (SCF)/c-kit signaling pathway in the pathogenesis of FD was systematically elucidated, and it was proposed that the Chai Gui Hewei Decoction repaired the ICC network through a three-fold mechanism: 1) Bupleurum saponin activated the c-kit receptor to promote the proliferation of ICC;2) Cinnamomum cassiae ingredient inhibited the tumor necrosis factor-α (TNF-α) to improve the inflammation microenvironment;3) Paeonia lactiflora glucoside regulates 5-hydroxytryptamine (5-HT) signaling to restore the balance of brain-gut axis. Based on the theory of “liver depression and spleen deficiency-ICC dysfunction”, a new hypothesis of multi-target repair of gastrointestinal pacing network by traditional Chinese herbal medicine compound was constructed, which provides a theoretical basis for the combination of Chinese and Western medicine.展开更多
The SKP1 gene is an important component of the SCF(SKP1-Cullin1-F-box)complex and serves as a bridge connecting the F-box and Cullin1genes(F-box-SKP1-Cullin1).The pattern of S-RNase being ubiquitously labelled by the ...The SKP1 gene is an important component of the SCF(SKP1-Cullin1-F-box)complex and serves as a bridge connecting the F-box and Cullin1genes(F-box-SKP1-Cullin1).The pattern of S-RNase being ubiquitously labelled by the SCF complex and degraded by the 26S protease accounts for the bulk of the available self-incompatibility studies.In this study,15 ClSKP1s from the‘Xiangshui'lemon genome and ubiquitome exist in the same SKP1 conserved domain(CD)as SKP1s in other species.The q PCR results showed that SKP1-6 and SKP1-14 have tissue expression patterns specific for expression in pollen.In addition,SKP1-6 and SKP1-14 in the stigma,style and ovary were significantly upregulated after self-pollination compared to those after cross-pollination.A subcellular location showed that SKP1-6 and SKP1-14 were located in the nucleus.In addition,yeast two-hybrid(Y2H)assays,bimolecular fluorescence complementation(BiFC)and luciferase complementation imaging(LCI)assays showed that SKP1-6 interacted with F-box1,F-box33,F-box34,F-box17,F-box19,Cullin1-2 and 26S proteasome subunit 4 homolog A(26S PS4HA).SKP1-14 interacted with F-box17,F-box19,F-box35,Cullin1-2 and 26S PS4HA.The interaction of Cullin1-2 and the F-box with SKP1 as a bridge was verified by a yeast three-hybrid experiment.The ability of S3-RNase to inhibit pollen and pollen tube growth and development was assessed using in vitro pollen co-culture experiments with recombinant S3-RNase proteins.Overall,this study provides important experimental evidence and theoretical basis for understanding the mechanism of self-incompatibility in plants by revealing the key role of the SCF complex in‘Xiangshui'lemon,which is bridged by ClSKP1-6,in self-incompatibility.The results of this study are of great significance for the future indepth exploration of the molecular mechanism of the SCF complex and its wide application in the self-incompatibility of plants.展开更多
胃肠动力障碍性疾病(disorders of gastrointestinal motility,DGIM)是指因胃肠动力失常引起的以消化道症状为主要临床表现的疾病。DGIM的发病率有逐年升高的趋势,降低人们生活质量的同时,还增加了社会的医疗支出。DGIM的发病机制尚未...胃肠动力障碍性疾病(disorders of gastrointestinal motility,DGIM)是指因胃肠动力失常引起的以消化道症状为主要临床表现的疾病。DGIM的发病率有逐年升高的趋势,降低人们生活质量的同时,还增加了社会的医疗支出。DGIM的发病机制尚未完全阐明,多项研究表明其发生发展与胃肠道动力异常关系密切。胃肠动力与胃肠道Cajal间质细胞(ICC)密切相关。ICC是胃肠道产生慢波运动的特殊细胞,ICC数量和形态的保持又与SCF/c-kit信号通路密切相关。近年来的研究表明,SCF/c-kit信号通路的异常改变可导致DGIM的发生。目前,DGIM的西医治疗主要为促动力及抑酸等对症治疗,在一定程度上能够缓解DGIM的症状,但常伴有不良反应,使药物的应用范围受限,而大量研究证实,中药、中药单体或成分、复方、中医外治法均可通过调节SCF/c-kit信号通路,有效改善胃肠动力的同时,不良反应较小。虽然中医药通过调控SCF/c-kit信号通路干预DGIM具有西医难以取代的独特优势,但目前中医药调控SCF/c-kit信号通路治疗DGIM系统综述仍较少。文章就SCF/c-kit信号通路与DGIM之间的相关性、中医药调控SCF/c-kit信号通路干预DGIM的作用机制进行系统性总结,旨在为临床治疗DGIM及新药研发提供新思考。展开更多
基金supported by the National Natural Science Foundation of China (32172599)the Beijing Rural Revitalization Agricultural Science and Technology Project (NY2401080000)+3 种基金the Beijing Agriculture Innovation Consortium (BAIC012025)the support from the Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Cropsthe Engineering Research Center of Breeding and Propagation of Horticultural Cropsthe 2115 Talent Development Program of China Agricultural University
文摘Ubiquitination,a critical post-translational modification,plays a pivotal role in fine tuning the immune responses of plants.The tomato(Solanum lycopersicum)suffers significant yield and quality losses caused by the devastating pathogen Botrytis cinerea.We have discovered the role of SlRAE1,a gene encoding an E3 ubiquitin ligase,as a pivotal negative regulator of resistance to B.cinerea.SlRAE1 interacts with SlSKP1,a component of the SKP1–Cullin1–F-box(SCF)complex,to modulate the protein stability of the transcription factor SlWRKY1 through the 26S proteasome pathway.SlWRKY1 targets and inhibits the transcription of SlJAZ7,a suppressor of jasmonic acid(JA)signaling,thereby activating the JA-induced defense system and affecting tomato susceptibility to B.cinerea.The resistance enhancement observed with knock-out SlRAE1 was reduced when SlWRKY1 was also knocked out,highlighting SlWRKY1's role in SlRAE1's regulation of tomato defense against B.cinerea.Our findings elucidate the defense mechanism in tomato and suggest that targeting SlRAE1,by modulating SlWRKY1 stability,could help to develop resistant tomato varieties.These insights have broader implications for using gene-editing technologies to enhance crop defense against fungi.
文摘功能性消化不良(FD)的胃肠动力障碍与Cajal间质细胞(ICC)网络损伤密切相关。文章系统阐述干细胞因子(SCF)/c-kit信号通路在FD发病中的核心作用,提出柴桂和胃方通过三重机制修复ICC网络:1) 柴胡皂苷激活c-kit受体促ICC增殖;2) 桂枝成分抑制肿瘤坏死因子-α (TNF-α)改善炎症微环境;3) 白芍苷调节5-羟色胺(5-HT)信号恢复脑肠轴平衡。基于“肝郁脾虚-ICC功能障碍”病机对应理论,构建中药复方多靶点修复胃肠起搏网络的新假说,为中西医结合治疗提供理论依据。Gastrointestinal dyskinesia in functional dyspepsia (FD) is closely related to the damage of Cajal interstitial cell (ICC) network. In this paper, the central role of the stem cell factor (SCF)/c-kit signaling pathway in the pathogenesis of FD was systematically elucidated, and it was proposed that the Chai Gui Hewei Decoction repaired the ICC network through a three-fold mechanism: 1) Bupleurum saponin activated the c-kit receptor to promote the proliferation of ICC;2) Cinnamomum cassiae ingredient inhibited the tumor necrosis factor-α (TNF-α) to improve the inflammation microenvironment;3) Paeonia lactiflora glucoside regulates 5-hydroxytryptamine (5-HT) signaling to restore the balance of brain-gut axis. Based on the theory of “liver depression and spleen deficiency-ICC dysfunction”, a new hypothesis of multi-target repair of gastrointestinal pacing network by traditional Chinese herbal medicine compound was constructed, which provides a theoretical basis for the combination of Chinese and Western medicine.
基金supported by grants from the National Natural Science Foundation of China(Grant No.31960585)Science and Technology Major Project of Guangxi(Grant No.Guike AA22068092)+1 种基金Guangxi Science and Technology Vanguard Special Action Project(Grant No.202204)State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources(Grant Nos.SKLCUSA-a201906,SKLCU-SA-c201901)。
文摘The SKP1 gene is an important component of the SCF(SKP1-Cullin1-F-box)complex and serves as a bridge connecting the F-box and Cullin1genes(F-box-SKP1-Cullin1).The pattern of S-RNase being ubiquitously labelled by the SCF complex and degraded by the 26S protease accounts for the bulk of the available self-incompatibility studies.In this study,15 ClSKP1s from the‘Xiangshui'lemon genome and ubiquitome exist in the same SKP1 conserved domain(CD)as SKP1s in other species.The q PCR results showed that SKP1-6 and SKP1-14 have tissue expression patterns specific for expression in pollen.In addition,SKP1-6 and SKP1-14 in the stigma,style and ovary were significantly upregulated after self-pollination compared to those after cross-pollination.A subcellular location showed that SKP1-6 and SKP1-14 were located in the nucleus.In addition,yeast two-hybrid(Y2H)assays,bimolecular fluorescence complementation(BiFC)and luciferase complementation imaging(LCI)assays showed that SKP1-6 interacted with F-box1,F-box33,F-box34,F-box17,F-box19,Cullin1-2 and 26S proteasome subunit 4 homolog A(26S PS4HA).SKP1-14 interacted with F-box17,F-box19,F-box35,Cullin1-2 and 26S PS4HA.The interaction of Cullin1-2 and the F-box with SKP1 as a bridge was verified by a yeast three-hybrid experiment.The ability of S3-RNase to inhibit pollen and pollen tube growth and development was assessed using in vitro pollen co-culture experiments with recombinant S3-RNase proteins.Overall,this study provides important experimental evidence and theoretical basis for understanding the mechanism of self-incompatibility in plants by revealing the key role of the SCF complex in‘Xiangshui'lemon,which is bridged by ClSKP1-6,in self-incompatibility.The results of this study are of great significance for the future indepth exploration of the molecular mechanism of the SCF complex and its wide application in the self-incompatibility of plants.
文摘胃肠动力障碍性疾病(disorders of gastrointestinal motility,DGIM)是指因胃肠动力失常引起的以消化道症状为主要临床表现的疾病。DGIM的发病率有逐年升高的趋势,降低人们生活质量的同时,还增加了社会的医疗支出。DGIM的发病机制尚未完全阐明,多项研究表明其发生发展与胃肠道动力异常关系密切。胃肠动力与胃肠道Cajal间质细胞(ICC)密切相关。ICC是胃肠道产生慢波运动的特殊细胞,ICC数量和形态的保持又与SCF/c-kit信号通路密切相关。近年来的研究表明,SCF/c-kit信号通路的异常改变可导致DGIM的发生。目前,DGIM的西医治疗主要为促动力及抑酸等对症治疗,在一定程度上能够缓解DGIM的症状,但常伴有不良反应,使药物的应用范围受限,而大量研究证实,中药、中药单体或成分、复方、中医外治法均可通过调节SCF/c-kit信号通路,有效改善胃肠动力的同时,不良反应较小。虽然中医药通过调控SCF/c-kit信号通路干预DGIM具有西医难以取代的独特优势,但目前中医药调控SCF/c-kit信号通路治疗DGIM系统综述仍较少。文章就SCF/c-kit信号通路与DGIM之间的相关性、中医药调控SCF/c-kit信号通路干预DGIM的作用机制进行系统性总结,旨在为临床治疗DGIM及新药研发提供新思考。
