DNA methylation plays a vital role in the regulation of gene expression in response to environmental stress. However, little is known about the effect of DNA methylation on the cassava polyploidy. In the present study...DNA methylation plays a vital role in the regulation of gene expression in response to environmental stress. However, little is known about the effect of DNA methylation on the cassava polyploidy. In the present study, methylation-sensitive amplified polymorphisms （MSAP） were used to investigate DNA methylation profiles of cassava polyploidy following cold treatment to identify candidate genes involved in response to cold stress. The result showed that the genome-wide DNA methylation polymorphisms accounted for 34.02%-42.56% in SC8 and its autotetraploid exposed to 5 ~C for 2, 8, 24 and 48 h, respectively. The methylation levels of SC8 at 2 h-cold stress were the highest during 48h under cold treatments. With the time extension within 48 h under cold stress, the methylation levels gradually decreased to the same level as the control but DNA methylation levels of cassava autotetraploid were stable within 48 h. For future analysis of the methylation extent, the cold stress induced more DNA methylation than demethylation in SC8, where DNA methylation was consistent with demethylation in its autotetraploid. The expression analysis demonstrated increase in the transcription of one methylated gene and decrease in the transcription of two demethylated genes. The results revealed that gene methylations in specific sites would be a rapidly epigenetic response to cold stress, further elucidating the methylation functions in its autotetraploid.展开更多
1概况ISO/TC10于1947年建立,工作对象主要是机械制图、建筑制图、船舶制图、土木制图及电气制图等各类制图标准化技术委员会其中SC8分技术委员会创立于1982年。TC10/SC8分委员会秘书处设在瑞典,我国是积极成员P,主要的领域是建筑制图的...1概况ISO/TC10于1947年建立,工作对象主要是机械制图、建筑制图、船舶制图、土木制图及电气制图等各类制图标准化技术委员会其中SC8分技术委员会创立于1982年。TC10/SC8分委员会秘书处设在瑞典,我国是积极成员P,主要的领域是建筑制图的符号、简化画法、标识、表示方法、计算机CAD中的图层、设备和建筑管理元数据文件等方面的内容。目前ISO/TC10/SC8有一个工作组WG16 :New forms of constructiondocumentation (新形式建筑文件)。展开更多
第22届ISO/TC34/SC8国际茶叶标准化会议于3月31-4月2日在西子湖畔召开。大会就十几项议题进行了深入的讨论,特别是在绿茶国际标准中的儿茶素与茶多酚比值指标争执较大。中国代表团本着对中国以及全球茶产业负责任的态度,力促会议最终达...第22届ISO/TC34/SC8国际茶叶标准化会议于3月31-4月2日在西子湖畔召开。大会就十几项议题进行了深入的讨论,特别是在绿茶国际标准中的儿茶素与茶多酚比值指标争执较大。中国代表团本着对中国以及全球茶产业负责任的态度,力促会议最终达成以下决议:1、茶叶中的茶氨酸测量方法可行性研究(ISO/ TC 34/SC 8 Resolution 151:Feasibility study to measure theanine in tea):展开更多
文摘DNA methylation plays a vital role in the regulation of gene expression in response to environmental stress. However, little is known about the effect of DNA methylation on the cassava polyploidy. In the present study, methylation-sensitive amplified polymorphisms （MSAP） were used to investigate DNA methylation profiles of cassava polyploidy following cold treatment to identify candidate genes involved in response to cold stress. The result showed that the genome-wide DNA methylation polymorphisms accounted for 34.02%-42.56% in SC8 and its autotetraploid exposed to 5 ~C for 2, 8, 24 and 48 h, respectively. The methylation levels of SC8 at 2 h-cold stress were the highest during 48h under cold treatments. With the time extension within 48 h under cold stress, the methylation levels gradually decreased to the same level as the control but DNA methylation levels of cassava autotetraploid were stable within 48 h. For future analysis of the methylation extent, the cold stress induced more DNA methylation than demethylation in SC8, where DNA methylation was consistent with demethylation in its autotetraploid. The expression analysis demonstrated increase in the transcription of one methylated gene and decrease in the transcription of two demethylated genes. The results revealed that gene methylations in specific sites would be a rapidly epigenetic response to cold stress, further elucidating the methylation functions in its autotetraploid.
文摘1概况ISO/TC10于1947年建立,工作对象主要是机械制图、建筑制图、船舶制图、土木制图及电气制图等各类制图标准化技术委员会其中SC8分技术委员会创立于1982年。TC10/SC8分委员会秘书处设在瑞典,我国是积极成员P,主要的领域是建筑制图的符号、简化画法、标识、表示方法、计算机CAD中的图层、设备和建筑管理元数据文件等方面的内容。目前ISO/TC10/SC8有一个工作组WG16 :New forms of constructiondocumentation (新形式建筑文件)。
文摘第22届ISO/TC34/SC8国际茶叶标准化会议于3月31-4月2日在西子湖畔召开。大会就十几项议题进行了深入的讨论,特别是在绿茶国际标准中的儿茶素与茶多酚比值指标争执较大。中国代表团本着对中国以及全球茶产业负责任的态度,力促会议最终达成以下决议:1、茶叶中的茶氨酸测量方法可行性研究(ISO/ TC 34/SC 8 Resolution 151:Feasibility study to measure theanine in tea):
