The SQUAMOSA promoter binding protein （SBP）-box genes encode a kind of plant-specific transcription factors （TFs） and play important roles in the regulation of plant development. In this study, a genome-wide chara...The SQUAMOSA promoter binding protein （SBP）-box genes encode a kind of plant-specific transcription factors （TFs） and play important roles in the regulation of plant development. In this study, a genome-wide characterization of this family was conducted in maize （Zea mays）. Thirty-one SBP-box genes were identified to be distributed in nine chromosomes and 16 of them were complementary to the mature ZmmiR156 sequences. All the Z. mays SBP （ZmSBP） genes were classified into two clusters with eight subgroups according to the phylogenetic analysis of proteins, which were consistent with the pattern of exon-intron structures. The phylogenetic tree of the ZmSBP, Oryza sativa SBP-like （OsSPL） and Arabidopsis thaliana SBP-like （AtSPL） genes were constructed and all the SBP-box genes were divided into eight groups, which was the same as the classification of ZmSBP genes. The comparision of the expression profiles of all SBP-box genes in these three species indicated that most orthologous genes had similar expression patterns. The results from this study provided a basic understanding of the ZmSBP genes and might facilitate future researches for elucidating the SBP-box genes function in maize.展开更多
SQUAMOSA PROMOTER BINDING PROTEIN box(SBP-box)基因编码一类植物特有的转录因子,其功能涉及植物生长发育的诸多过程,包括植物叶片的形态建成、胚胎发生、营养生长到生殖生长的更替等,在作物遗传改良方面具有重要的应用价值。本研究...SQUAMOSA PROMOTER BINDING PROTEIN box(SBP-box)基因编码一类植物特有的转录因子,其功能涉及植物生长发育的诸多过程,包括植物叶片的形态建成、胚胎发生、营养生长到生殖生长的更替等,在作物遗传改良方面具有重要的应用价值。本研究克隆了高粱SbSBP5基因,分析了其序列特征及其在不同逆境下的表达模式。结果表明SbSBP5编码884个氨基酸,含有一个典型SBP结构域,其启动子区含有ABRE、ARE和MBS等逆境相关顺式元件;SbSBP5可受PEG和外源ABA诱导上调表达,表达峰值时分别是对照的12倍和11倍,SbSBP5启动子在转基因拟南芥中可驱动GUS报告基因表达,并且其活性可受干旱和ABA诱导增强,分别是对照的3倍和4倍。本研究结果进一步证明SbSBP5参与了植株对干旱和外源ABA的响应,为深入研究SbSBP5的功能奠定了基础。展开更多
基金support by the National Natural Science Foundation of China(31200911,31101576)the China Postdoctoral Science Foundation(20100471197,201104475)the Research Fund for the Doctoral Program of Higher Education of China(20110146120040)
文摘The SQUAMOSA promoter binding protein （SBP）-box genes encode a kind of plant-specific transcription factors （TFs） and play important roles in the regulation of plant development. In this study, a genome-wide characterization of this family was conducted in maize （Zea mays）. Thirty-one SBP-box genes were identified to be distributed in nine chromosomes and 16 of them were complementary to the mature ZmmiR156 sequences. All the Z. mays SBP （ZmSBP） genes were classified into two clusters with eight subgroups according to the phylogenetic analysis of proteins, which were consistent with the pattern of exon-intron structures. The phylogenetic tree of the ZmSBP, Oryza sativa SBP-like （OsSPL） and Arabidopsis thaliana SBP-like （AtSPL） genes were constructed and all the SBP-box genes were divided into eight groups, which was the same as the classification of ZmSBP genes. The comparision of the expression profiles of all SBP-box genes in these three species indicated that most orthologous genes had similar expression patterns. The results from this study provided a basic understanding of the ZmSBP genes and might facilitate future researches for elucidating the SBP-box genes function in maize.
文摘SQUAMOSA PROMOTER BINDING PROTEIN box(SBP-box)基因编码一类植物特有的转录因子,其功能涉及植物生长发育的诸多过程,包括植物叶片的形态建成、胚胎发生、营养生长到生殖生长的更替等,在作物遗传改良方面具有重要的应用价值。本研究克隆了高粱SbSBP5基因,分析了其序列特征及其在不同逆境下的表达模式。结果表明SbSBP5编码884个氨基酸,含有一个典型SBP结构域,其启动子区含有ABRE、ARE和MBS等逆境相关顺式元件;SbSBP5可受PEG和外源ABA诱导上调表达,表达峰值时分别是对照的12倍和11倍,SbSBP5启动子在转基因拟南芥中可驱动GUS报告基因表达,并且其活性可受干旱和ABA诱导增强,分别是对照的3倍和4倍。本研究结果进一步证明SbSBP5参与了植株对干旱和外源ABA的响应,为深入研究SbSBP5的功能奠定了基础。
