Over-expression of the pathway specific positive regulator gene is an effective way to activate silent gene cluster.In the curret study,the SARP family regulatory gene,vasR2,was over-expressed in strain Verrucosispora...Over-expression of the pathway specific positive regulator gene is an effective way to activate silent gene cluster.In the curret study,the SARP family regulatory gene,vasR2,was over-expressed in strain Verrucosispora sp.NS0172 and the cryptic gene cluster responsible for the biosynthesis of pentaketide ansamycin was partially activated.Two tetraketides(1 and 2)and a triketide(3)ansamycins,together with five known compounds(4–8),were isolated and elucidated from strain NS0172OEvasR2.Their NMR data were completely assigned by analysis of their HR-ESI-MS and 1H,13C NMR,HMQC,HMBC and 1H-1H COSY spectra.展开更多
随着国家统计局编制的sarp 2000 for windows程序在统计报表工作中的应用,极大地方便了基层统计对统计报表的数据处理,但是在sarp2000程序里统计报表都是以数据形式存在的,我们不能直接对统计报表进行查看,更不能按年份查询统计指标,那...随着国家统计局编制的sarp 2000 for windows程序在统计报表工作中的应用,极大地方便了基层统计对统计报表的数据处理,但是在sarp2000程序里统计报表都是以数据形式存在的,我们不能直接对统计报表进行查看,更不能按年份查询统计指标,那么,我们如何解决这个问题呢?下面,简单谈一下统计电子年鉴的制作.展开更多
基金supported by the National Natural Science Foundation of China(Nos.81673317,81530091,and 31570039)the Open Funding Project of State Key Laboratory of Microbial Metabolite Funding(No.MMLKF17-09)the Program for Changjiang Scholars and Innovative Research Team in University(No.IRT_17R68).
文摘Over-expression of the pathway specific positive regulator gene is an effective way to activate silent gene cluster.In the curret study,the SARP family regulatory gene,vasR2,was over-expressed in strain Verrucosispora sp.NS0172 and the cryptic gene cluster responsible for the biosynthesis of pentaketide ansamycin was partially activated.Two tetraketides(1 and 2)and a triketide(3)ansamycins,together with five known compounds(4–8),were isolated and elucidated from strain NS0172OEvasR2.Their NMR data were completely assigned by analysis of their HR-ESI-MS and 1H,13C NMR,HMQC,HMBC and 1H-1H COSY spectra.
文摘随着国家统计局编制的sarp 2000 for windows程序在统计报表工作中的应用,极大地方便了基层统计对统计报表的数据处理,但是在sarp2000程序里统计报表都是以数据形式存在的,我们不能直接对统计报表进行查看,更不能按年份查询统计指标,那么,我们如何解决这个问题呢?下面,简单谈一下统计电子年鉴的制作.
