Fallen leaves of Ficus altissima, F. virens, F. benjamina, F. fistulosa and F. semicordata, were collected in Chiang Mai Province in northern Thailand and examined for fungi. Eighty taxa were identified, comprising 56...Fallen leaves of Ficus altissima, F. virens, F. benjamina, F. fistulosa and F. semicordata, were collected in Chiang Mai Province in northern Thailand and examined for fungi. Eighty taxa were identified, comprising 56 anamorphic taxa, 23 ascomycetes and l basidiomycete. Common fungal species occurring on five host species with high frequency of occurrence were Beltraniella nilgirica, Lasiodiplodia theobromae, Ophioceras leptosporum, Periconia byssoides and Septonema harknessi. Colletotrichum and Stachybotrys were also common genera. The leaves of different Ficus species supported diverse fungal taxa, and the fungal assemblages on the different hosts showed varying overlap. The fungal diversity of saprobes at the host species level is discussed.展开更多
A fungus similar to the monotypic genus Natarajania,isolated from dead wood and collected in Thailand,is reported.Analysis of partial ribosomal LSU and a protein coding gene(RPB2)demonstrated that the new isolate belo...A fungus similar to the monotypic genus Natarajania,isolated from dead wood and collected in Thailand,is reported.Analysis of partial ribosomal LSU and a protein coding gene(RPB2)demonstrated that the new isolate belonged to Stilbosporaceae,Diaporthales and genetically different from N.indica.It is unique in producing synnematous conidiophores,smooth-walled conidiogenous cells and a flared collarette but lacks an elongated collar-canal which is distinct in the type species.Therefore,sequence data and morphological traits are used to introduce the new species,Natarajania thailandica.展开更多
To study the relationship between zooplankton community structure and environmental factors and water quality in the Harbin Section of the Songhua River,investigations were carried out in June,August,and October 2011....To study the relationship between zooplankton community structure and environmental factors and water quality in the Harbin Section of the Songhua River,investigations were carried out in June,August,and October 2011.Canonical correspondence analysis(CCA) and saprobic indices were used to process and analyze the data.Seasonal variability was identified as a significant source of variation,which explains the fluctuation in zooplankton density.In autumn,the dry season,water residence time increased and zooplankton biomass and abundance accumulated in the slow flowing waters.Zooplankton abundance increased when food conditions improved.Therefore,the total zooplankton abundance in autumn is much higher than that in spring and summer.According to the saprobic indices,all the sample sites had mesosaprobic water and water quality was worse in autumn.CCA revealed that temperature accounted for most of the spatial variation in the zooplankton community.Moreover,pH,dissolved oxygen saturation,and turbidity were important factors affecting zooplankton community distribution.展开更多
The present condition of Southern Kazakhstan natural reservoirs is represented in the article. The research was held in summer time of 2010. Quantitative and qualitative features and dominating types of zooplankton of...The present condition of Southern Kazakhstan natural reservoirs is represented in the article. The research was held in summer time of 2010. Quantitative and qualitative features and dominating types of zooplankton of the individual reservoirs are shown. It is detected that the waters of the South Kazakhstan oblast （region） are inhabited by 70 types of zooplankton organisms of different taxonomic groups, whose association to certain reservoir depends on the hydrochemical indicators of the aquatic environment.展开更多
Sexual morph of didymellaceous taxa are characterized by their ascomata with relatively thin peridium,cylindric-clavate to clavate,short-pedicellate or apedicellate asci,hyaline to brown,1-septate to muriform ascospor...Sexual morph of didymellaceous taxa are characterized by their ascomata with relatively thin peridium,cylindric-clavate to clavate,short-pedicellate or apedicellate asci,hyaline to brown,1-septate to muriform ascospores.Its asexual morphs are coelomycetous and comprising pycnidial or acervulus conidiomata,phialidic,hyaline conidiogenous cells and hyaline or pale brown,septate or aseptate conidia.The majority of these cosmopolitan species are plant associated fungi which can be pathogens on a wide range of hosts and some species are of particular relevance for quarantine measures.Recent studies have significantly improved the taxonomy and systematics of didymellaceous taxa based on molecular phylogenetics.In contrast to the accurate and detailed studies on the asexual morphs which are common obligate pathogens,information on their usually saprobic sexual morphs is still limited.Among these phenotypically diverse species,spore characteristics are quite unique as most have hyaline spores with 0-1 septum,while only Neomicrosphaeropsis and Didymellocamarosporium are reported as producing pigmented,muriform spores.These dematiaceous muriform spores are characteristic of a considerable number of species that may be quite divergent in other characters.During taxonomic investigations on the diversity of didymellaceous taxa,we have isolated species from Alhagi pseudalhagi,Coronilla emerus,Cytisus sp.,Elaeagnus angustifolia and Spartium junceum in Italy,Russia and Uzbekistan.A comprehensive phylogeny,based on four loci(ITS,LSU,rpb2 and tub2)is used to infer species relationships.Comprehensive morphological descriptions and in-depth phylogenetic investigations of five new species viz.Ascochyta coronillae-emeri,Microsphaeropsis spartii-juncei,Neomicrosphaeropsis alhagi-pseudalhagi,N.cytisicola and N.elaeagni are presented.展开更多
The present paper provides a checklist of reported species from genera Ascobolus Pers.and Saccobolus Boud.in India with one additional first record from India,Saccobolus depauperatus.In addition,A.brassicae,A.furfurac...The present paper provides a checklist of reported species from genera Ascobolus Pers.and Saccobolus Boud.in India with one additional first record from India,Saccobolus depauperatus.In addition,A.brassicae,A.furfuraceus,A.foliicola,A.saccharifereus,Saccobolus depauperatus and S.glaber are reported for the first time from Kashmir,India and descriptions and photomicroplates of all said species are provided for their easy identification.展开更多
We identified a sporocarp as Agrocybe dura growing next to a living corn using PacBio sequencing.The mycoparasitism of Trichoderma spp.on A.dura were revealed by the co-occurrence network analysis.For long-read HTS da...We identified a sporocarp as Agrocybe dura growing next to a living corn using PacBio sequencing.The mycoparasitism of Trichoderma spp.on A.dura were revealed by the co-occurrence network analysis.For long-read HTS data,we updated a bioinformatic pipeline to enhance fungal taxonomic resolution.In forests,fungal sporocarps house the diverse fungicolous fungi;however,the relationships of sporocarps and associated fungal communities are rarely explored in agroecosystems.In a corn field near Gongzhuling City,Jilin Province,China,we found an epigeous sporocarp with agaricoid morphology that could grow next to the living corn plants.Using PacBio metabarcoding combined with an updated bioinformatic pipeline,we surveyed the fungal community profile along its cap,rhizomorph and hyphosphere soil at a much-improved taxonomic resolution.We identified the sporocarp,at a high probability,as Agrocybe dura,and this mushroom was significantly negatively correlated with Trichoderma hamatum and T.harzianum in the co-occurrence network.Fungal diversity in hyphosphere habitat was significantly higher than that in cap and rhizomorph habitats.Consistent with the pattern in fungal diversity,the node number,edge number,network diameter and average degree were significantly higher in hyphosphere habitat than other habitats.However,both the negative and positive cohesion were significantly higher in rhizomorph habitat than other habitats.Moreover,the z-c plot identified A.dura as the only network hub,linking multiple fungal species.The results give us a glimpse of the ecological relevance of saprobic mushrooms across the extensive northeastern black soil region of China.Our findings will aid in the assessment and forecasting of fungal diversity hotspots and their relationships with soil fertility in the‘Golden Corn Belt’of northeast China.展开更多
Refined conversion factors for soil fungal biomarkers are proposed.High interspecific variability is present in all fungal biomarkers.A modeling approach supports the validity of biomarker estimates in diverse soils.I...Refined conversion factors for soil fungal biomarkers are proposed.High interspecific variability is present in all fungal biomarkers.A modeling approach supports the validity of biomarker estimates in diverse soils.ITS1 copies vary strongly,but are fungal-specific with least phylogenetic bias.A combination of fungal biomarkers will reveal soil fungal physiology and activity.The abundances of fungi and bacteria in soil are used as simple predictors for carbon dynamics,and represent widely available microbial traits.Soil biomarkers serve as quantitative estimates of these microbial groups,though not quantifying microbial biomass per se.The accurate conversion to microbial carbon pools,and an understanding of its comparability among soils is therefore needed.We refined conversion factors for classical fungal biomarkers,and evaluated the application of quantitative PCR(qPCR,rDNA copies)as a biomarker for soil fungi.Based on biomarker contents in pure fungal cultures of 30 isolates tested here,combined with comparable published datasets,we propose average conversion factors of 95.3 g fungal C g^(−1) ergosterol,32.0 mg fungal Cμmol−1 PLFA 18:2ω6,9 and 0.264 pg fungal C ITS1 DNA copy−1.As expected,interspecific variability was most pronounced in rDNA copies,though qPCR results showed the least phylogenetic bias.A modeling approach based on exemplary agricultural soils further supported the hypothesis that high diversity in soil buffers against biomarker variability,whereas also phylogenetic biases impact the accuracy of comparisons in biomarker estimates.Our analyses suggest that qPCR results cover the fungal community in soil best,though with a variability only partly offset in highly diverse soils.PLFA 18:2ω6,9 and ergosterol represent accurate biomarkers to quantify Ascomycota and Basidiomycota.To conclude,the ecological interpretation and coverage of biomarker data prior to their application in global models is important,where the combination of different biomarkers may be most insightful.展开更多
Helicosporous hyphomycetes are a fungal group with similar coiled or spiral conidial morphology but significant differences in genetic information,resulting in them being distributed in different phyla,classes,orders,...Helicosporous hyphomycetes are a fungal group with similar coiled or spiral conidial morphology but significant differences in genetic information,resulting in them being distributed in different phyla,classes,orders,and families.However,there are no comprehensive studies for this group.In this study,we have systematically organized the helicosporous taxa,revised their classification and provided a modern taxonomic framework based on morphology and phylogeny.This group encompasses 112 genera and 474 species distributed in three phyla,ten classes,20 orders and 25 families,while some taxa are classified as incertae sedis within Ascomycota.The illustrations and notes for all helicosporous genera and the drawings of the type or representative species that have not been collected and examined are provided.Additionally,a phylogenetic taxonomic distribution of helicosporous families based on maximum likelihood analysis of LSU,ITS,SSU,tef1-α,and rpb2 sequence data is provided.In addition,we obtained 160 new collections from China and Thailand.Based on molecular evidence and morphological characteristics,six new helicosporous genera viz.Acrohelicosporium,Hyalohelicoon,Hyalohelisphora,Hyalotubeufia,Pseudocirrenalia,and Pseudohelicosporium and 53 new species,viz.Acrohelicosporium abundatum,A.aquaticum,A.guizhouense,A.viridisporum,Berkleasmium hainanense,Helicoma brunneum,H.astrictum,H.dipterocarpi,H.guizhouense,H.sclerotiferum,H.tropicum,H.yinggelingense,Helicoma yunnanense,Helicosporium acropleurogenum,H.brunneisporum,H.changjiangense,H.jiangkouense,H.latisporum,H.ramosiphorum,Helicotubeufia laxisporum,Hya-lohelicoon multiseptatum,Hyalohelisphora lignicola,Neohelicomyces acropleurogenus,N.aseptatus,N.edgeworthiae,N.guttulatus,N.lignicola,N.macrosporus,N.qixingyaensis,N.xiayadongensis,N.yunnanensis,N.baihualingense,Neoheli-cosporium hainanense,N.jianfenglingense,N.latisporum,Parahelicomyces latisporus,Pa.laxisporus,Pa.parvisporus,Paratrimmatostroma helicosporum,Pleurohelicosporium brunneisporum,Pl.hyalosporum,Pl.multiseptatum,Pseudocir-renalia aquialpina,Pseudohelicosporium irregular,Ps.laxisporum,Pseudotubeufia dematiolaxispora,Sclerococcum astric-tum,Tubeufia acropleurogena,T.baomeilingensis,T.denticulate,T.subrenispora,T.tropica,and Troposporella guttulata are introduced.Additionally,37 new records and 22 known species are reported.Furthermore,we synonymize 30 species and provide 30 new combinations.Complete descriptions,illustrations,notes,and phylogenetic trees based on maximum likelihood and Bayesian analyses of LSU,ITS,mtSSU,SSU,tef1-α,and rpb2 sequence data are also provided for the new taxa,new combinations,new records,and known species.A worldwide checklist comprising the distribution,habitat,and availability of molecular data for the accepted helicosporous species is also provided.展开更多
The genus Thelonectria and closely related species with cylindrocarpon-like asexual states are a group of perithecial ascomycetes in the family Nectriaceae that occur as saprobes and in few cases as pathogens of hardw...The genus Thelonectria and closely related species with cylindrocarpon-like asexual states are a group of perithecial ascomycetes in the family Nectriaceae that occur as saprobes and in few cases as pathogens of hardwood trees,shrubs or other plants.Although a key component of forest ecosystems around the world,species relationships and distributions of these fungi are largely unknown.The objectives of this study were to:1)infer species rank phylogenetic relationships of the genus Thelonectria and closely related species with cylindrocarpon-like asexual states and test the monophyly of each of the groups studied;2)delimit taxa establishing taxon circumscriptions;3)resolve nomenclatural issues by identifying redundantly used names and synonyms;and 4)provide an updated outline to the genus,geographical distributions data and identification tools,specifically diagnostic keys and molecular data that can be used as molecular barcodes.The recovered consensus phylogeny resulted in a narrow circumscription of the genus Thelonectria,based on the type T.discophora,excluding one of the common species T.jungneri.According to the phylogenetic analyses,T.jungneri belongs in a segregate clade that should be recognized as a different genus.In the genus Thelonectria,a total of four new species and three new combinations are recognized.Additionally,three new genera,closely related to Thelonectria,are described to accommodate species displaying a morphological resemblance to those of Thelonectria:Cinnamomeonectria gen.nov.with C.cinnamomea as type species,Macronectria gen.nov.with M.jungneri as type species and including four additional newly described species,and Tumenectria gen.nov.with T.laetidisca as type species.展开更多
Fungi that are barely lichenized or non-lichenized and closely related to lichenized taxa,the so-called borderline fungi,are an important element in reconstructing the evolutionary history of lichenized lineages.Artho...Fungi that are barely lichenized or non-lichenized and closely related to lichenized taxa,the so-called borderline fungi,are an important element in reconstructing the evolutionary history of lichenized lineages.Arthoniaceae is a prime example including non-lichenized,saprotrophic lineages which potentially were precursors to lichenized taxa.In this study,we focused on saprotrophic species of Arthonia sensu lato,including new sequence data for Arthonia pinastri.We obtained fresh material of this taxon from a living branch of Fraxinus ornus in Italy to assess its taxonomic status and to elucidate its phylogenetic relationships within Arthonia.Thin sections of the thallus and ascomata of A.pinastri confirmed the absence of a photobiont.Maximum likelihood and Bayesian analyses of combined mtSSU,nuLSU and RPB2 sequence data placed the species close to A.dispersa(barely lichenized or non-lichenized)and A.punctiformis(non-lichenized)in a clade closely related to Arthonia sensu stricto,and the A.pinastri clade is here resurrected under the name Naevia.Ancestral character state analysis within a broader context of Arthoniales does not support the saprotrophic lifestyle to be a plesiomorphic feature,but suggests loss of lichenization in Naevia,as well as loss and possible regain in a second clade containing saprotrophic species and including taxa resembling Mycoporum,underlining the evolutionary plasticity of Arthoniales.These two clades constitute model taxa to further investigate the evolution of alternative biological lifestyles within the context of chiefly lichenized taxa.展开更多
Hot desert regions are undoubtedly challenging to fungal survival on the Arabian Peninsula.Fungi are,however,recognized as the most stress-resistant organisms among all eukaryotes,which could be a result of the rapid ...Hot desert regions are undoubtedly challenging to fungal survival on the Arabian Peninsula.Fungi are,however,recognized as the most stress-resistant organisms among all eukaryotes,which could be a result of the rapid evolution of distinct species.Our current understanding of these microorganisms is derived from studies examining only a fraction of the overall fungal diversity.Therefore,further studies are needed to understand the diversity of fungi in desert regions.This paper highlights the taxonomy of several unusual fungal genera collected in a range of aquatic and terrestrial environments in Oman.These taxa were identified based on phylogenetic analyses of nuclear ribosomal DNA(rDNA)(LSU,SSU and ITS)and protein-coding genes(TEF,RPB2 and TUB),plus morphological comparisons.Phylogenetic analyses,including the presently recognized genera in Patellariales resulted in splitting the order into four clades in Dothideomycetes.The new family Holmiellaceae and new order Holmiellales are introduced to include Holmiella.Species of Homortomycetaceae form a well-supported and distinct clade and raise it to Homortomycetales ordo novus.Omania(Halojulellaceae),Desertiserpentica(Lophiostomata-ceae)and Montanitestudina(Testudinaceae)are described as novel genera in Dothideomycetes.A synnematous hyphomycete with basidiomycetous affinity(Corticiales)was also identified and described as Basidiodesertica.Additionally,an asexual morph was observed for Holmiella junipericola;Patellaria quercus is synonymized under P.atrata,and seven new species are described.展开更多
The family Pleosporaceae includes numerous saprobic,opportunistic human,and plant pathogenic taxa.The classification of genera and species Pleosporaceae has been a major challenge due to the lack of a clear understand...The family Pleosporaceae includes numerous saprobic,opportunistic human,and plant pathogenic taxa.The classification of genera and species Pleosporaceae has been a major challenge due to the lack of a clear understanding of the importance of the morphological characters used to distinguish taxa as well as the lack of reference strains.Recent treatments concluded that Pleospora and some other genera in Pleosporaceae are likely polyphyletic.In order to establish the evolutionary relationships and to resolve the polyphyletic nature of Pleospora and allied genera,we sequenced the 18S nrDNA,28S nrDNA,ITS,GAPDH,RPB2 and TEF1-alpha gene regions of Pleosporaceae species and phylogenetically analysed this data.Multigene phylogenies strongly support the monophyletic nature of Pleosporaceae among the other families in Pleosporales,and the acceptance of the genera Alternaria,Bipolaris,Clathrospora,Comoclathris,Curvularia,Dactuliophora,Decorospora,Diademosa,Exserohilum,Extrawettsteinina,Gibbago,Neocamarosporium,Paradendryphiella,Platysporoides,Pleospora,Porocercospora,Pseudoyuconia and Pyrenophora.Austropleospora,Dendryphion,Edenia and Macrospora are excluded from the family based on morphology coupled with molecular data.Two novel species,Alternaria murispora in this paper and Comoclathris sedi are introduced.The sexual morph of Alternaria alternata is re-described and illustrated using modern concepts from fresh collections.The paraphyletic nature of Pleospora is resolved based on the available morpho-molecular data,but further sampling with fresh collections,reference or ex-type strains and molecular data are needed to obtain a natural classification of genera and the family.展开更多
Pestalotiopsis is a taxonomically confused,pathogenic and chemically creative genus requiring a critical reexamination using a multi-gene phylogeny based on ex-type and ex-epitype cultures.In this study 40 isolates of...Pestalotiopsis is a taxonomically confused,pathogenic and chemically creative genus requiring a critical reexamination using a multi-gene phylogeny based on ex-type and ex-epitype cultures.In this study 40 isolates of Pestalotiopsis,comprised of 28 strains collected from living and dead plant material of various host plants from China were studied by means of morphology and analysis of ITS,β–tubulin and tef1 gene sequence data.Based on molecular and morphological data we describe 14 new species(Pestalotiopsis asiatica,P.chinensis,P.chrysea,P.clavata,P.diversiseta,P.ellipsospora,P.inflexa,P.intermedia,P.linearis,P.rosea,P.saprophyta,P.umberspora,P.unicolor and P.verruculosa)and three species are epitypified(P.adusta,P.clavispora and P.foedans).Of the 10 gene regions(ACT,β-tubulin,CAL,GPDH,GS,ITS,LSU,RPB 1,SSU and tef1)utilized to resolve cryptic Pestalotiopsis species,ITS,β–tubulin and tef1 proved to be the better markers.The other gene regions were less useful due to poor success in PCR amplification and/or in their ability to resolve species boundaries.As a single gene tef1 met the requirements for an ideal candidate and functions well for species delimitation due to its better species resolution and PCR success.Althoughβ-tubulin showed fairly good differences among species,a combination of ITS,β-tubulin and tef1 gene data gave the best resolution as compared to single gene analysis.This work provides a backbone tree for 22 ex-type/epitypified species of Pestalotiopsis and can be used in future studies of the genus.展开更多
This paper provides illustrated descriptions of micro-fungi newly found on Pandanaceae in China and Thailand.The fungi are accommodated in 31 families.New taxa described include a new family,seven new genera,65 new sp...This paper provides illustrated descriptions of micro-fungi newly found on Pandanaceae in China and Thailand.The fungi are accommodated in 31 families.New taxa described include a new family,seven new genera,65 new species,16 previously known species.A new family:Malaysiascaceae(Glomerellales).New genera are Acremoniisimulans(Plec-tosphaerellaceae),Pandanaceomyces,Pseudoachroiostachy(Nectriaceae),Pseudohyaloseta(Niessliaceae),Pseudoor-natispora(Stachybotriaceae)and Yunnanomyces(Sympoventuriaceae).New species are Acremoniisimulans thailandensis,Beltrania krabiensis,Beltraniella pandanicola,B.thailandicus,Canalisporium krabiense,C.thailandensis,Clonostachys krabiensis,Curvularia chonburiensis,C.pandanicola,C.thailandicum,C.xishuangbannaensis,Cylindrocladiella xishuangbannaensis,Dictyochaeta pandanicola,Dictyocheirospora nabanheensis,D.pandanicola,D.xishuangbannaen-sis,Dictyosporium appendiculatum,Di.guttulatum,Di.hongkongensis,Di.krabiense,Di.pandanicola,Distoseptispora thailandica,D.xishuangbannaensis,Helicoma freycinetiae,Hermatomyces biconisporus,Lasiodiplodia chonburiensis,L.pandanicola,Lasionectria krabiense,Menisporopsis pandanicola,Montagnula krabiensis,Musicillium pandanicola,Neofusicoccum pandanicola,Neohelicomyces pandanicola,Neooccultibambusa thailandensis,Neopestalotiopsis chiang-maiensis,N.pandanicola,N.phangngaensis,Pandanaceomyces krabiensis,Paracylindrocarpon nabanheensis,P.pan-danicola,P.xishuangbannaensis,Parasarcopodium hongkongensis,Pestalotiopsis krabiensis,P.pandanicola,Polyplosphaeria nabanheensis,P.pandanicola,P.xishuangbannaensis,Pseudoachroiostachys krabiense,Pseu-doberkleasmium pandanicola,Pseudochaetosphaeronema pandanicola,Pseudohyaloseta pandanicola,Pseudoornatispora krabiense,Pseudopithomyces pandanicola,Rostriconidium pandanicola,Sirastachys phangngaensis,Stictis pandanicola,Terriera pandanicola,Thozetella pandanicola,Tubeufia freycinetiae,T.parvispora,T.pandanicola,Vermiculariopsiella hongkongensis,Volutella krabiense,V.thailandensis and Yunnanomyces pandanicola.Previous studies of micro-fungi on Pandanaceae have not included phylogenetic support.Inspiration for this study came from the book Fungi Associated with Pandanaceae by Whitton,McKenzie and Hyde in 2012.Both studies reveal that the micro-fungi on Pandanaceae is particularly rich in hyphomycetes.All data presented herein are based on morphological examination of specimens,coupled with phylogenetic sequence data to better integrate taxa into appropriate taxonomic ranks and infer their evolu-tionary relationships.展开更多
基金the Mushroom Research Centre, Chiang Mai,Thailand and the National Natural Science Foundation of China (No. 30670072)the Natural Science Foundation of Zhejiang Province of China (No. Y350568)
文摘Fallen leaves of Ficus altissima, F. virens, F. benjamina, F. fistulosa and F. semicordata, were collected in Chiang Mai Province in northern Thailand and examined for fungi. Eighty taxa were identified, comprising 56 anamorphic taxa, 23 ascomycetes and l basidiomycete. Common fungal species occurring on five host species with high frequency of occurrence were Beltraniella nilgirica, Lasiodiplodia theobromae, Ophioceras leptosporum, Periconia byssoides and Septonema harknessi. Colletotrichum and Stachybotrys were also common genera. The leaves of different Ficus species supported diverse fungal taxa, and the fungal assemblages on the different hosts showed varying overlap. The fungal diversity of saprobes at the host species level is discussed.
基金Thailand Research Fund(TRF)grant no RSA5980068(Biodiversity,phylogeny and role of fungal endophytes on above parts of Rhizophora apiculata)is acknowledged.Monika C.Dayarathne would like to acknowledge projects:National Natural Science Foundation of China(Nos.31972222,31560489)Talent project of Guizhou Science and Technology Cooperation Platform([2017]5788-5 and[2019]5641)and Guizhou ScienceTechnology Department International Cooperation Basic project([2018]5806).
文摘A fungus similar to the monotypic genus Natarajania,isolated from dead wood and collected in Thailand,is reported.Analysis of partial ribosomal LSU and a protein coding gene(RPB2)demonstrated that the new isolate belonged to Stilbosporaceae,Diaporthales and genetically different from N.indica.It is unique in producing synnematous conidiophores,smooth-walled conidiogenous cells and a flared collarette but lacks an elongated collar-canal which is distinct in the type species.Therefore,sequence data and morphological traits are used to introduce the new species,Natarajania thailandica.
基金Supported by the National Natural Science Foundation of China(No.41271106)
文摘To study the relationship between zooplankton community structure and environmental factors and water quality in the Harbin Section of the Songhua River,investigations were carried out in June,August,and October 2011.Canonical correspondence analysis(CCA) and saprobic indices were used to process and analyze the data.Seasonal variability was identified as a significant source of variation,which explains the fluctuation in zooplankton density.In autumn,the dry season,water residence time increased and zooplankton biomass and abundance accumulated in the slow flowing waters.Zooplankton abundance increased when food conditions improved.Therefore,the total zooplankton abundance in autumn is much higher than that in spring and summer.According to the saprobic indices,all the sample sites had mesosaprobic water and water quality was worse in autumn.CCA revealed that temperature accounted for most of the spatial variation in the zooplankton community.Moreover,pH,dissolved oxygen saturation,and turbidity were important factors affecting zooplankton community distribution.
文摘The present condition of Southern Kazakhstan natural reservoirs is represented in the article. The research was held in summer time of 2010. Quantitative and qualitative features and dominating types of zooplankton of the individual reservoirs are shown. It is detected that the waters of the South Kazakhstan oblast （region） are inhabited by 70 types of zooplankton organisms of different taxonomic groups, whose association to certain reservoir depends on the hydrochemical indicators of the aquatic environment.
基金the Committee for Coordination Science and Technology Development under the Cabinet of Ministers of Uzbekistan for research support(#P3-2014-0830174425).
文摘Sexual morph of didymellaceous taxa are characterized by their ascomata with relatively thin peridium,cylindric-clavate to clavate,short-pedicellate or apedicellate asci,hyaline to brown,1-septate to muriform ascospores.Its asexual morphs are coelomycetous and comprising pycnidial or acervulus conidiomata,phialidic,hyaline conidiogenous cells and hyaline or pale brown,septate or aseptate conidia.The majority of these cosmopolitan species are plant associated fungi which can be pathogens on a wide range of hosts and some species are of particular relevance for quarantine measures.Recent studies have significantly improved the taxonomy and systematics of didymellaceous taxa based on molecular phylogenetics.In contrast to the accurate and detailed studies on the asexual morphs which are common obligate pathogens,information on their usually saprobic sexual morphs is still limited.Among these phenotypically diverse species,spore characteristics are quite unique as most have hyaline spores with 0-1 septum,while only Neomicrosphaeropsis and Didymellocamarosporium are reported as producing pigmented,muriform spores.These dematiaceous muriform spores are characteristic of a considerable number of species that may be quite divergent in other characters.During taxonomic investigations on the diversity of didymellaceous taxa,we have isolated species from Alhagi pseudalhagi,Coronilla emerus,Cytisus sp.,Elaeagnus angustifolia and Spartium junceum in Italy,Russia and Uzbekistan.A comprehensive phylogeny,based on four loci(ITS,LSU,rpb2 and tub2)is used to infer species relationships.Comprehensive morphological descriptions and in-depth phylogenetic investigations of five new species viz.Ascochyta coronillae-emeri,Microsphaeropsis spartii-juncei,Neomicrosphaeropsis alhagi-pseudalhagi,N.cytisicola and N.elaeagni are presented.
文摘The present paper provides a checklist of reported species from genera Ascobolus Pers.and Saccobolus Boud.in India with one additional first record from India,Saccobolus depauperatus.In addition,A.brassicae,A.furfuraceus,A.foliicola,A.saccharifereus,Saccobolus depauperatus and S.glaber are reported for the first time from Kashmir,India and descriptions and photomicroplates of all said species are provided for their easy identification.
基金supported by the National Program on Key Basic Research Project(Grant No.2022YFD1500202)the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDA28020202)the National Natural Science Foundation of China(Grant No.42277308).
文摘We identified a sporocarp as Agrocybe dura growing next to a living corn using PacBio sequencing.The mycoparasitism of Trichoderma spp.on A.dura were revealed by the co-occurrence network analysis.For long-read HTS data,we updated a bioinformatic pipeline to enhance fungal taxonomic resolution.In forests,fungal sporocarps house the diverse fungicolous fungi;however,the relationships of sporocarps and associated fungal communities are rarely explored in agroecosystems.In a corn field near Gongzhuling City,Jilin Province,China,we found an epigeous sporocarp with agaricoid morphology that could grow next to the living corn plants.Using PacBio metabarcoding combined with an updated bioinformatic pipeline,we surveyed the fungal community profile along its cap,rhizomorph and hyphosphere soil at a much-improved taxonomic resolution.We identified the sporocarp,at a high probability,as Agrocybe dura,and this mushroom was significantly negatively correlated with Trichoderma hamatum and T.harzianum in the co-occurrence network.Fungal diversity in hyphosphere habitat was significantly higher than that in cap and rhizomorph habitats.Consistent with the pattern in fungal diversity,the node number,edge number,network diameter and average degree were significantly higher in hyphosphere habitat than other habitats.However,both the negative and positive cohesion were significantly higher in rhizomorph habitat than other habitats.Moreover,the z-c plot identified A.dura as the only network hub,linking multiple fungal species.The results give us a glimpse of the ecological relevance of saprobic mushrooms across the extensive northeastern black soil region of China.Our findings will aid in the assessment and forecasting of fungal diversity hotspots and their relationships with soil fertility in the‘Golden Corn Belt’of northeast China.
基金funding by the Deutsche Forschungsgemeinschaft(DFG,grant number 465123751,SPP2322 SoilSystems)supported by DFG grant HE 6183/5-1 and SM by MA4436/1-5.
文摘Refined conversion factors for soil fungal biomarkers are proposed.High interspecific variability is present in all fungal biomarkers.A modeling approach supports the validity of biomarker estimates in diverse soils.ITS1 copies vary strongly,but are fungal-specific with least phylogenetic bias.A combination of fungal biomarkers will reveal soil fungal physiology and activity.The abundances of fungi and bacteria in soil are used as simple predictors for carbon dynamics,and represent widely available microbial traits.Soil biomarkers serve as quantitative estimates of these microbial groups,though not quantifying microbial biomass per se.The accurate conversion to microbial carbon pools,and an understanding of its comparability among soils is therefore needed.We refined conversion factors for classical fungal biomarkers,and evaluated the application of quantitative PCR(qPCR,rDNA copies)as a biomarker for soil fungi.Based on biomarker contents in pure fungal cultures of 30 isolates tested here,combined with comparable published datasets,we propose average conversion factors of 95.3 g fungal C g^(−1) ergosterol,32.0 mg fungal Cμmol−1 PLFA 18:2ω6,9 and 0.264 pg fungal C ITS1 DNA copy−1.As expected,interspecific variability was most pronounced in rDNA copies,though qPCR results showed the least phylogenetic bias.A modeling approach based on exemplary agricultural soils further supported the hypothesis that high diversity in soil buffers against biomarker variability,whereas also phylogenetic biases impact the accuracy of comparisons in biomarker estimates.Our analyses suggest that qPCR results cover the fungal community in soil best,though with a variability only partly offset in highly diverse soils.PLFA 18:2ω6,9 and ergosterol represent accurate biomarkers to quantify Ascomycota and Basidiomycota.To conclude,the ecological interpretation and coverage of biomarker data prior to their application in global models is important,where the combination of different biomarkers may be most insightful.
基金funded by the National Natural Science Foundation of China(NSFC 32360011)the Second Qinghai-Tibet Plateau Scientific Investigation and Research Program(STEP)Program(Grant No.2019QZKK0503)Guizhou Institute of Technology High-Level Talent Research Start-up Project(2023GCC069).
文摘Helicosporous hyphomycetes are a fungal group with similar coiled or spiral conidial morphology but significant differences in genetic information,resulting in them being distributed in different phyla,classes,orders,and families.However,there are no comprehensive studies for this group.In this study,we have systematically organized the helicosporous taxa,revised their classification and provided a modern taxonomic framework based on morphology and phylogeny.This group encompasses 112 genera and 474 species distributed in three phyla,ten classes,20 orders and 25 families,while some taxa are classified as incertae sedis within Ascomycota.The illustrations and notes for all helicosporous genera and the drawings of the type or representative species that have not been collected and examined are provided.Additionally,a phylogenetic taxonomic distribution of helicosporous families based on maximum likelihood analysis of LSU,ITS,SSU,tef1-α,and rpb2 sequence data is provided.In addition,we obtained 160 new collections from China and Thailand.Based on molecular evidence and morphological characteristics,six new helicosporous genera viz.Acrohelicosporium,Hyalohelicoon,Hyalohelisphora,Hyalotubeufia,Pseudocirrenalia,and Pseudohelicosporium and 53 new species,viz.Acrohelicosporium abundatum,A.aquaticum,A.guizhouense,A.viridisporum,Berkleasmium hainanense,Helicoma brunneum,H.astrictum,H.dipterocarpi,H.guizhouense,H.sclerotiferum,H.tropicum,H.yinggelingense,Helicoma yunnanense,Helicosporium acropleurogenum,H.brunneisporum,H.changjiangense,H.jiangkouense,H.latisporum,H.ramosiphorum,Helicotubeufia laxisporum,Hya-lohelicoon multiseptatum,Hyalohelisphora lignicola,Neohelicomyces acropleurogenus,N.aseptatus,N.edgeworthiae,N.guttulatus,N.lignicola,N.macrosporus,N.qixingyaensis,N.xiayadongensis,N.yunnanensis,N.baihualingense,Neoheli-cosporium hainanense,N.jianfenglingense,N.latisporum,Parahelicomyces latisporus,Pa.laxisporus,Pa.parvisporus,Paratrimmatostroma helicosporum,Pleurohelicosporium brunneisporum,Pl.hyalosporum,Pl.multiseptatum,Pseudocir-renalia aquialpina,Pseudohelicosporium irregular,Ps.laxisporum,Pseudotubeufia dematiolaxispora,Sclerococcum astric-tum,Tubeufia acropleurogena,T.baomeilingensis,T.denticulate,T.subrenispora,T.tropica,and Troposporella guttulata are introduced.Additionally,37 new records and 22 known species are reported.Furthermore,we synonymize 30 species and provide 30 new combinations.Complete descriptions,illustrations,notes,and phylogenetic trees based on maximum likelihood and Bayesian analyses of LSU,ITS,mtSSU,SSU,tef1-α,and rpb2 sequence data are also provided for the new taxa,new combinations,new records,and known species.A worldwide checklist comprising the distribution,habitat,and availability of molecular data for the accepted helicosporous species is also provided.
基金This study was funded by a grant from United States National Science Foundation(PEET program)DEB-0925696。
文摘The genus Thelonectria and closely related species with cylindrocarpon-like asexual states are a group of perithecial ascomycetes in the family Nectriaceae that occur as saprobes and in few cases as pathogens of hardwood trees,shrubs or other plants.Although a key component of forest ecosystems around the world,species relationships and distributions of these fungi are largely unknown.The objectives of this study were to:1)infer species rank phylogenetic relationships of the genus Thelonectria and closely related species with cylindrocarpon-like asexual states and test the monophyly of each of the groups studied;2)delimit taxa establishing taxon circumscriptions;3)resolve nomenclatural issues by identifying redundantly used names and synonyms;and 4)provide an updated outline to the genus,geographical distributions data and identification tools,specifically diagnostic keys and molecular data that can be used as molecular barcodes.The recovered consensus phylogeny resulted in a narrow circumscription of the genus Thelonectria,based on the type T.discophora,excluding one of the common species T.jungneri.According to the phylogenetic analyses,T.jungneri belongs in a segregate clade that should be recognized as a different genus.In the genus Thelonectria,a total of four new species and three new combinations are recognized.Additionally,three new genera,closely related to Thelonectria,are described to accommodate species displaying a morphological resemblance to those of Thelonectria:Cinnamomeonectria gen.nov.with C.cinnamomea as type species,Macronectria gen.nov.with M.jungneri as type species and including four additional newly described species,and Tumenectria gen.nov.with T.laetidisca as type species.
基金We thank the Thailand Research Fund(“The future of specialist fungi in a changing climate:baseline data for generalist and specialist fungi associated with ants,Rhododendron species and Dracaena species DBG6080013”and“Impact of climate change on fungal diversity and biogeography in the Greater Mekong Subregion RDG6130001”)“The 2019 high-end foreign expert introduction plan to Kunming Institute of Botany(granted by the Ministry of Science and Technology of the People’s Republic of China(Grant Number G20190139006))for funding this research.S.C.Karunarathna would like to thank the CAS President’s International Fellowship Initiative(PIFI)under the following grant:2018PC0006 and the National Science Foundation of China(NSFC,project code 31851110759)+1 种基金We also thank Udeni Jayalal,Nalin Wijayawardene,Ming-Ying Zhang,Mohammed Warris,Lee B.G and Eleni Gentekaki for their support during this research.Dhanushka Wanasinghe would like to thank CAS President’s International Fellowship Initiative(PIFI)for funding his postdoctoral research(Number 2019PC0008)the National Science Foundation of China and the Chinese Academy of Sciences for financial support under the following Grants:41761144055,41771063 and Y4ZK111B01.We also extend our gratitude to Teuvo Ahti,David Hawksworth,Pier-Luigi Nimis and Rikard Sundin and Mats Wedin who helped to clarify the nomenclatural status of the genus Naevia.
文摘Fungi that are barely lichenized or non-lichenized and closely related to lichenized taxa,the so-called borderline fungi,are an important element in reconstructing the evolutionary history of lichenized lineages.Arthoniaceae is a prime example including non-lichenized,saprotrophic lineages which potentially were precursors to lichenized taxa.In this study,we focused on saprotrophic species of Arthonia sensu lato,including new sequence data for Arthonia pinastri.We obtained fresh material of this taxon from a living branch of Fraxinus ornus in Italy to assess its taxonomic status and to elucidate its phylogenetic relationships within Arthonia.Thin sections of the thallus and ascomata of A.pinastri confirmed the absence of a photobiont.Maximum likelihood and Bayesian analyses of combined mtSSU,nuLSU and RPB2 sequence data placed the species close to A.dispersa(barely lichenized or non-lichenized)and A.punctiformis(non-lichenized)in a clade closely related to Arthonia sensu stricto,and the A.pinastri clade is here resurrected under the name Naevia.Ancestral character state analysis within a broader context of Arthoniales does not support the saprotrophic lifestyle to be a plesiomorphic feature,but suggests loss of lichenization in Naevia,as well as loss and possible regain in a second clade containing saprotrophic species and including taxa resembling Mycoporum,underlining the evolutionary plasticity of Arthoniales.These two clades constitute model taxa to further investigate the evolution of alternative biological lifestyles within the context of chiefly lichenized taxa.
基金the CAS President’s International Fellowship Initiative(number 2019PC0008)the 64th batch of China Postdoctoral Science Foundation(grant no.2018M643549)Postdoctoral Fund from Human Resources and Social Security Bureau of Yunnan Province and the National Science Foundation of China and the Chinese Academy of Sciences(Grant No.:41761144055).
文摘Hot desert regions are undoubtedly challenging to fungal survival on the Arabian Peninsula.Fungi are,however,recognized as the most stress-resistant organisms among all eukaryotes,which could be a result of the rapid evolution of distinct species.Our current understanding of these microorganisms is derived from studies examining only a fraction of the overall fungal diversity.Therefore,further studies are needed to understand the diversity of fungi in desert regions.This paper highlights the taxonomy of several unusual fungal genera collected in a range of aquatic and terrestrial environments in Oman.These taxa were identified based on phylogenetic analyses of nuclear ribosomal DNA(rDNA)(LSU,SSU and ITS)and protein-coding genes(TEF,RPB2 and TUB),plus morphological comparisons.Phylogenetic analyses,including the presently recognized genera in Patellariales resulted in splitting the order into four clades in Dothideomycetes.The new family Holmiellaceae and new order Holmiellales are introduced to include Holmiella.Species of Homortomycetaceae form a well-supported and distinct clade and raise it to Homortomycetales ordo novus.Omania(Halojulellaceae),Desertiserpentica(Lophiostomata-ceae)and Montanitestudina(Testudinaceae)are described as novel genera in Dothideomycetes.A synnematous hyphomycete with basidiomycetous affinity(Corticiales)was also identified and described as Basidiodesertica.Additionally,an asexual morph was observed for Holmiella junipericola;Patellaria quercus is synonymized under P.atrata,and seven new species are described.
基金MFLU grant number 56101020032 is thanked for supporting studies on Dothideomycetes.We are grateful to the Mushroom Research Foundation,Chiang Rai,Thailand for supporting studies on Dothideomycetes.Kevin D.Hyde thanks the Chinese Academy of Sciences,project number 2013T2S0030,for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botany.Jian-Chu Xu and Peter E Mortimer would like to thank Humidtropics,a CGIAR Research Program that aims to develop new opportunities for improved livelihoods in a sustainable environment,for partially funding this work.H.A Ariyawansa and J.C.Kang are grateful to the agricultural science and technology foundation of Guizhou province(Nos.NY[2013]3042),the international collaboration plan of Guizhou province(No.G[2012]7006)and the innovation team construction for science and technology of Guizhou province(No.[2012]4007)from the Science and Technology Department of Guizhou province,China.Hiran Ariyawansa is grateful to A.D Ariyawansa,D.M.K Ariyawansa and Dhanuska Udayanga for their valuable suggestions.E.B.Gareth Jones is supported by the Distinguished Scientist Fellowship Program(DSFP),King Saud University,Saudi Arabia.
文摘The family Pleosporaceae includes numerous saprobic,opportunistic human,and plant pathogenic taxa.The classification of genera and species Pleosporaceae has been a major challenge due to the lack of a clear understanding of the importance of the morphological characters used to distinguish taxa as well as the lack of reference strains.Recent treatments concluded that Pleospora and some other genera in Pleosporaceae are likely polyphyletic.In order to establish the evolutionary relationships and to resolve the polyphyletic nature of Pleospora and allied genera,we sequenced the 18S nrDNA,28S nrDNA,ITS,GAPDH,RPB2 and TEF1-alpha gene regions of Pleosporaceae species and phylogenetically analysed this data.Multigene phylogenies strongly support the monophyletic nature of Pleosporaceae among the other families in Pleosporales,and the acceptance of the genera Alternaria,Bipolaris,Clathrospora,Comoclathris,Curvularia,Dactuliophora,Decorospora,Diademosa,Exserohilum,Extrawettsteinina,Gibbago,Neocamarosporium,Paradendryphiella,Platysporoides,Pleospora,Porocercospora,Pseudoyuconia and Pyrenophora.Austropleospora,Dendryphion,Edenia and Macrospora are excluded from the family based on morphology coupled with molecular data.Two novel species,Alternaria murispora in this paper and Comoclathris sedi are introduced.The sexual morph of Alternaria alternata is re-described and illustrated using modern concepts from fresh collections.The paraphyletic nature of Pleospora is resolved based on the available morpho-molecular data,but further sampling with fresh collections,reference or ex-type strains and molecular data are needed to obtain a natural classification of genera and the family.
基金We thank Thailand Research Fund(grant:BRG5280002)The National Research Council of Thailand(grant for Pestalotiopsis No:55201020008)+4 种基金Mae Fah Luang University(grant for Pestalotiopsis No:55101020004)The National Natural Science Foundation of China(grant:30930005)the Knowledge Innovation Program of the Chinese Academy of Sciences(grant:KSCX2-YW-Z0935)Mushroom Research Foundation,Chiang Mai,ThailandState Key Laboratory of Mycology,Institute of Microbiology,Chinese Academy of Sciences,Beijing,China and the King Saud University for supporting this research.
文摘Pestalotiopsis is a taxonomically confused,pathogenic and chemically creative genus requiring a critical reexamination using a multi-gene phylogeny based on ex-type and ex-epitype cultures.In this study 40 isolates of Pestalotiopsis,comprised of 28 strains collected from living and dead plant material of various host plants from China were studied by means of morphology and analysis of ITS,β–tubulin and tef1 gene sequence data.Based on molecular and morphological data we describe 14 new species(Pestalotiopsis asiatica,P.chinensis,P.chrysea,P.clavata,P.diversiseta,P.ellipsospora,P.inflexa,P.intermedia,P.linearis,P.rosea,P.saprophyta,P.umberspora,P.unicolor and P.verruculosa)and three species are epitypified(P.adusta,P.clavispora and P.foedans).Of the 10 gene regions(ACT,β-tubulin,CAL,GPDH,GS,ITS,LSU,RPB 1,SSU and tef1)utilized to resolve cryptic Pestalotiopsis species,ITS,β–tubulin and tef1 proved to be the better markers.The other gene regions were less useful due to poor success in PCR amplification and/or in their ability to resolve species boundaries.As a single gene tef1 met the requirements for an ideal candidate and functions well for species delimitation due to its better species resolution and PCR success.Althoughβ-tubulin showed fairly good differences among species,a combination of ITS,β-tubulin and tef1 gene data gave the best resolution as compared to single gene analysis.This work provides a backbone tree for 22 ex-type/epitypified species of Pestalotiopsis and can be used in future studies of the genus.
文摘This paper provides illustrated descriptions of micro-fungi newly found on Pandanaceae in China and Thailand.The fungi are accommodated in 31 families.New taxa described include a new family,seven new genera,65 new species,16 previously known species.A new family:Malaysiascaceae(Glomerellales).New genera are Acremoniisimulans(Plec-tosphaerellaceae),Pandanaceomyces,Pseudoachroiostachy(Nectriaceae),Pseudohyaloseta(Niessliaceae),Pseudoor-natispora(Stachybotriaceae)and Yunnanomyces(Sympoventuriaceae).New species are Acremoniisimulans thailandensis,Beltrania krabiensis,Beltraniella pandanicola,B.thailandicus,Canalisporium krabiense,C.thailandensis,Clonostachys krabiensis,Curvularia chonburiensis,C.pandanicola,C.thailandicum,C.xishuangbannaensis,Cylindrocladiella xishuangbannaensis,Dictyochaeta pandanicola,Dictyocheirospora nabanheensis,D.pandanicola,D.xishuangbannaen-sis,Dictyosporium appendiculatum,Di.guttulatum,Di.hongkongensis,Di.krabiense,Di.pandanicola,Distoseptispora thailandica,D.xishuangbannaensis,Helicoma freycinetiae,Hermatomyces biconisporus,Lasiodiplodia chonburiensis,L.pandanicola,Lasionectria krabiense,Menisporopsis pandanicola,Montagnula krabiensis,Musicillium pandanicola,Neofusicoccum pandanicola,Neohelicomyces pandanicola,Neooccultibambusa thailandensis,Neopestalotiopsis chiang-maiensis,N.pandanicola,N.phangngaensis,Pandanaceomyces krabiensis,Paracylindrocarpon nabanheensis,P.pan-danicola,P.xishuangbannaensis,Parasarcopodium hongkongensis,Pestalotiopsis krabiensis,P.pandanicola,Polyplosphaeria nabanheensis,P.pandanicola,P.xishuangbannaensis,Pseudoachroiostachys krabiense,Pseu-doberkleasmium pandanicola,Pseudochaetosphaeronema pandanicola,Pseudohyaloseta pandanicola,Pseudoornatispora krabiense,Pseudopithomyces pandanicola,Rostriconidium pandanicola,Sirastachys phangngaensis,Stictis pandanicola,Terriera pandanicola,Thozetella pandanicola,Tubeufia freycinetiae,T.parvispora,T.pandanicola,Vermiculariopsiella hongkongensis,Volutella krabiense,V.thailandensis and Yunnanomyces pandanicola.Previous studies of micro-fungi on Pandanaceae have not included phylogenetic support.Inspiration for this study came from the book Fungi Associated with Pandanaceae by Whitton,McKenzie and Hyde in 2012.Both studies reveal that the micro-fungi on Pandanaceae is particularly rich in hyphomycetes.All data presented herein are based on morphological examination of specimens,coupled with phylogenetic sequence data to better integrate taxa into appropriate taxonomic ranks and infer their evolu-tionary relationships.
