Change in catalase activity was examined in leaves of rice plant exposed to salinity. Depending on the method of preparation of crude protein extract from leaf and the constituents of the assay medium, a significant d...Change in catalase activity was examined in leaves of rice plant exposed to salinity. Depending on the method of preparation of crude protein extract from leaf and the constituents of the assay medium, a significant difference in enzyme activity was recorded. Inclusion of sorbitol or mannitol or sucrose in the extraction and enzyme assay medium enhanced the enzyme activity in salt-stressed samples by nearly 1.5-1.8 fold, compared to the activity found in un- stressed plants, which otherwise showed a 50% declined activity in leaf extract prepared in buffer solution and assayed in a medium depleted of these sugars. In view of the accumulation of osmolytes under saline condition, these observations suggest that the catalase activity is modulated by the osmolytes and maintains a high rate of hydrogen peroxide scavenging property in vivo and serves as the major antioxidant enzyme to scavenge the salt-induced formation of peroxide. Therefore, the salt-stress induced appearance of low activity of the enzyme under normal buffer extraction and assay conditions, as reported in literature may represent an apparent than for its real in vivo activity.展开更多
为研究多花黄精(Polygonatum cyrtonema)脱水反应元件结合蛋白(dehydration responsive element binding,DREB)基因家族的功能,基于多花黄精不同组织器官转录组、全长转录组数据对DREB家族成员进行鉴定,同时对其蛋白理化性质、系统进化...为研究多花黄精(Polygonatum cyrtonema)脱水反应元件结合蛋白(dehydration responsive element binding,DREB)基因家族的功能,基于多花黄精不同组织器官转录组、全长转录组数据对DREB家族成员进行鉴定,同时对其蛋白理化性质、系统进化特征、保守基序和蛋白结构进行分析;并结合FPKM值及qRT-PCR分析在不同器官、盐胁迫和PEG-6000模拟干旱胁迫下的表达量;通过农杆菌介导法转化洋葱,进行PcDREB10亚细胞定位;利用发根农杆菌诱导多花黄精毛状根,利用qRT-PCR分析过表达PcDREB10盐胁迫后PcDREB10/12/27的表达情况。研究结果表明:多花黄精DREB家族有35个成员,命名为PcDREB1~PcDREB35,均为亲水蛋白,且稳定性较低。PcDREB家族成员在不同器官中的表达不一致,具有组织特异性,且在盐胁迫及干旱胁迫下表达模式不同。亚细胞定位结果显示PcDREB10在细胞核上。发根农杆菌介导的过表达PcDREB10后,经高盐溶液处理,根系中PcDREB10/27上调表达,而PcDREB12下调表达,提示PcDREB10/12/27在多花黄精抵御盐胁迫的过程中可能发挥不同作用。展开更多
文摘Change in catalase activity was examined in leaves of rice plant exposed to salinity. Depending on the method of preparation of crude protein extract from leaf and the constituents of the assay medium, a significant difference in enzyme activity was recorded. Inclusion of sorbitol or mannitol or sucrose in the extraction and enzyme assay medium enhanced the enzyme activity in salt-stressed samples by nearly 1.5-1.8 fold, compared to the activity found in un- stressed plants, which otherwise showed a 50% declined activity in leaf extract prepared in buffer solution and assayed in a medium depleted of these sugars. In view of the accumulation of osmolytes under saline condition, these observations suggest that the catalase activity is modulated by the osmolytes and maintains a high rate of hydrogen peroxide scavenging property in vivo and serves as the major antioxidant enzyme to scavenge the salt-induced formation of peroxide. Therefore, the salt-stress induced appearance of low activity of the enzyme under normal buffer extraction and assay conditions, as reported in literature may represent an apparent than for its real in vivo activity.
文摘为研究多花黄精(Polygonatum cyrtonema)脱水反应元件结合蛋白(dehydration responsive element binding,DREB)基因家族的功能,基于多花黄精不同组织器官转录组、全长转录组数据对DREB家族成员进行鉴定,同时对其蛋白理化性质、系统进化特征、保守基序和蛋白结构进行分析;并结合FPKM值及qRT-PCR分析在不同器官、盐胁迫和PEG-6000模拟干旱胁迫下的表达量;通过农杆菌介导法转化洋葱,进行PcDREB10亚细胞定位;利用发根农杆菌诱导多花黄精毛状根,利用qRT-PCR分析过表达PcDREB10盐胁迫后PcDREB10/12/27的表达情况。研究结果表明:多花黄精DREB家族有35个成员,命名为PcDREB1~PcDREB35,均为亲水蛋白,且稳定性较低。PcDREB家族成员在不同器官中的表达不一致,具有组织特异性,且在盐胁迫及干旱胁迫下表达模式不同。亚细胞定位结果显示PcDREB10在细胞核上。发根农杆菌介导的过表达PcDREB10后,经高盐溶液处理,根系中PcDREB10/27上调表达,而PcDREB12下调表达,提示PcDREB10/12/27在多花黄精抵御盐胁迫的过程中可能发挥不同作用。
