In forage grasses, the nitrogen concentration is directly related to the nutritional value. The studies examined the hypothesis that global elevation of CO2 concentration probably affects the biomass, nitrogen (N) c...In forage grasses, the nitrogen concentration is directly related to the nutritional value. The studies examined the hypothesis that global elevation of CO2 concentration probably affects the biomass, nitrogen (N) concentration, and allocation and distribution patterns in the organs of forage grasses. While sainfoin (Onobrychis viciaefolia Scop.) seedlings grew on a low nutrient soil in closed chambers for 90 days, they were exposed to two CO2 concentrations (ambient or ambient+350 μmol mol^-1 CO2) without adding nutrients to them. After 90 days exposure to CO2, the biomasses of leaves, stems, and roots, and N concentrations and contents of different parts were measured. Compared with the ambient CO2 concentration, the elevated CO2 concentration increased the total dry matter by 25.07%, mainly due to the root and leaf having positive response to the elevated CO2 concentration. However, the elevated CO2 concentration did not change the proportions of the dry matters in different parts and the total plants compared with the ambient CO2 concentration. The elevated CO2 concentration lowered the N concentrations of the plant parts. Because the dry matter was higher, the elevated CO2 concentration had no effect on the N content in the plants compared to the ambient CO2 concentration. The elevated CO2 concentration promoted N allocations of the different parts significantly and increased N allocation of the underground part. The results have confirmed the previous suggestions that the elevated CO2 concentration stimulates plant biomass production and decreases the N concentrations of the plant parts.展开更多
To solve the problem of lack of water in arid areas and barren soil in the process of ecological restoration, the growth and drought resistance of Sainfoin (Onobrychis vidifolia Scop.) was studied by inoculation of ...To solve the problem of lack of water in arid areas and barren soil in the process of ecological restoration, the growth and drought resistance of Sainfoin (Onobrychis vidifolia Scop.) was studied by inoculation of AMF (arbuscular rnycorrhizal fungi), under drought stress. The results show that the inoculation of AMF can significantly improve the growth and mycorrhizal infection rate of Sainfoin and accelerate the absorption of water and nutrients by the roots compared with uninoculated plants. On the 40th day under drought stress, the relative water content, total nitrogen and total phosphorus content of the leaves of inoculated plants increased by 7.27%, 4.21% and 2.40%, respectively, the osmotic adjustment property in plant cells was improved, the protective enzyme system was improved and membrane lipid peroxida- tion was reduced. The inoculation of AMF can slow down the damage from drought, improve the drought resistance of Sainfoin, and will be of great significance to the ecologic restoration in arid areas.展开更多
The effects of replacing grass silage by sainfoin silage in a total mixed ration(TMR)based diet on fatty acid(FA)reticular inflow and milk FA profile of dairy cows was investigated.The experiment followed a crossover ...The effects of replacing grass silage by sainfoin silage in a total mixed ration(TMR)based diet on fatty acid(FA)reticular inflow and milk FA profile of dairy cows was investigated.The experiment followed a crossover design with 2 dietary treatments.The control diet consisted of grass silage,corn silage,concentrate and linseed.In the sainfoin diet,half of the grass silage was replaced by a sainfoin silage.Six rumen cannulated lactating multiparous dairy cows with a metabolic body weight of 132.5±3.6 kg BW^0.75,214±72 d in milk and an average milk production of 23.1±2.8 kg/d were used in the experiment.Cows were paired based on parity and milk production.Within pairs,cows were randomly assigned to either the control diet or the sainfoin diet for 2 experimental periods(29 d per period).In each period,the first 21 d,cows were housed individually in tie-stalls for adaptation,then next 4 d cows were housed individually in climate-controlled respiration chambers to measure CH4.During the last 4 d,cows were housed individually in tie stalls to measure milk FA profile and determine FA reticular inflow using the reticular sampling technique with Cr-ethylenediaminetetraacetic acid disodium salt dihydrate(EDTA)and Yb-acetate used as digesta flow markers.Although the dietary C18:3n-3 intake was lower(P=0.025)in the sainfoin diet group,the mono-unsaturated FA reticular inflow was greater(P=0.042)in cows fed the sainfoin diet.The reticular inflow of trans-9,trans-12-C18:2 and cis-12,trans-10 C18:2 was greater(P≤0.024)in the sainfoin diet group.The cows fed sainfoin diet had a lower(P≤0.038)apparent ruminal biohydrogenation of cis-9-C18:1 and C18:3n-3,compared to the cows fed the control diet.The sainfoin diet group had greater(P≤0.018)C18:3n-3 and cis-9,cis-12-C18:2 proportions in the milk FA profile compared to the control diet group.Transfer efficiencies from feed to milk of C18:2,C18:3n-3 and unsaturated FA were greater(P≤0.0179)for the sainfoin diet.Based on the results,it could be concluded that replacing grass silage by sainfoin silage in dairy cow rations reduces ruminal C18:3n-3 biohydrogenation and improves milk FA profile.展开更多
【目的】数量性状是红豆草DUS(Distinctness,Uniformity and Stability)测试指南中易受环境条件影响的性状。为了能够顺利开展红豆草DUS测试工作,需要对红豆草品种数量性状的表达状态进行适宜的分级。【方法】以76个红豆草(Onobrychis v...【目的】数量性状是红豆草DUS(Distinctness,Uniformity and Stability)测试指南中易受环境条件影响的性状。为了能够顺利开展红豆草DUS测试工作,需要对红豆草品种数量性状的表达状态进行适宜的分级。【方法】以76个红豆草(Onobrychis viciaefolia Scop.)种质资源为研究对象,对红豆草13个数量性状进行观测,选择合适的分级方法将各数量性状的表达状态进行合理的分级,为红豆草品种DUS测试指南研制提供技术支撑。【结果】13个数量性状的变异范围为6.36%~70.58%;采用最小显著差法,将正态分布的荚果长度分为3级,小叶数、复叶长度、穗长度分为5级,节间长度、荚果宽度分为7级,株高、百粒重分为9级;采用极差法,将非正态分布的分枝数分为7级,开花期、茎直径、叶片长度、叶片宽度分为9级。通过聚类分析可将红豆草资源分为3大类群,类群Ⅰ包含18份材料,占24%;类群Ⅱ包含23份材料,占30%;类群Ⅲ包含35份材料,占46%。【结论】研究初步建立了红豆草种质资源13个数量性状的分级指标,为中国红豆草DUS测试提供了科学依据。展开更多
基金supported by the Specialized Research Fund for the Doctoral Program of Higher Education(SRFDP200807181008)the Science Fund of Shaanxi Normal University for the Young Scholars,China
文摘In forage grasses, the nitrogen concentration is directly related to the nutritional value. The studies examined the hypothesis that global elevation of CO2 concentration probably affects the biomass, nitrogen (N) concentration, and allocation and distribution patterns in the organs of forage grasses. While sainfoin (Onobrychis viciaefolia Scop.) seedlings grew on a low nutrient soil in closed chambers for 90 days, they were exposed to two CO2 concentrations (ambient or ambient+350 μmol mol^-1 CO2) without adding nutrients to them. After 90 days exposure to CO2, the biomasses of leaves, stems, and roots, and N concentrations and contents of different parts were measured. Compared with the ambient CO2 concentration, the elevated CO2 concentration increased the total dry matter by 25.07%, mainly due to the root and leaf having positive response to the elevated CO2 concentration. However, the elevated CO2 concentration did not change the proportions of the dry matters in different parts and the total plants compared with the ambient CO2 concentration. The elevated CO2 concentration lowered the N concentrations of the plant parts. Because the dry matter was higher, the elevated CO2 concentration had no effect on the N content in the plants compared to the ambient CO2 concentration. The elevated CO2 concentration promoted N allocations of the different parts significantly and increased N allocation of the underground part. The results have confirmed the previous suggestions that the elevated CO2 concentration stimulates plant biomass production and decreases the N concentrations of the plant parts.
基金provided by the Science and Technology Major Project of Shanxi Province (No. 20121101008), is gratefully acknowledged
文摘To solve the problem of lack of water in arid areas and barren soil in the process of ecological restoration, the growth and drought resistance of Sainfoin (Onobrychis vidifolia Scop.) was studied by inoculation of AMF (arbuscular rnycorrhizal fungi), under drought stress. The results show that the inoculation of AMF can significantly improve the growth and mycorrhizal infection rate of Sainfoin and accelerate the absorption of water and nutrients by the roots compared with uninoculated plants. On the 40th day under drought stress, the relative water content, total nitrogen and total phosphorus content of the leaves of inoculated plants increased by 7.27%, 4.21% and 2.40%, respectively, the osmotic adjustment property in plant cells was improved, the protective enzyme system was improved and membrane lipid peroxida- tion was reduced. The inoculation of AMF can slow down the damage from drought, improve the drought resistance of Sainfoin, and will be of great significance to the ecologic restoration in arid areas.
基金financially supported by the European Commission Marie Curie Research Training Network grant“LegumePlus”(PITN-GA-2011-289377)
文摘The effects of replacing grass silage by sainfoin silage in a total mixed ration(TMR)based diet on fatty acid(FA)reticular inflow and milk FA profile of dairy cows was investigated.The experiment followed a crossover design with 2 dietary treatments.The control diet consisted of grass silage,corn silage,concentrate and linseed.In the sainfoin diet,half of the grass silage was replaced by a sainfoin silage.Six rumen cannulated lactating multiparous dairy cows with a metabolic body weight of 132.5±3.6 kg BW^0.75,214±72 d in milk and an average milk production of 23.1±2.8 kg/d were used in the experiment.Cows were paired based on parity and milk production.Within pairs,cows were randomly assigned to either the control diet or the sainfoin diet for 2 experimental periods(29 d per period).In each period,the first 21 d,cows were housed individually in tie-stalls for adaptation,then next 4 d cows were housed individually in climate-controlled respiration chambers to measure CH4.During the last 4 d,cows were housed individually in tie stalls to measure milk FA profile and determine FA reticular inflow using the reticular sampling technique with Cr-ethylenediaminetetraacetic acid disodium salt dihydrate(EDTA)and Yb-acetate used as digesta flow markers.Although the dietary C18:3n-3 intake was lower(P=0.025)in the sainfoin diet group,the mono-unsaturated FA reticular inflow was greater(P=0.042)in cows fed the sainfoin diet.The reticular inflow of trans-9,trans-12-C18:2 and cis-12,trans-10 C18:2 was greater(P≤0.024)in the sainfoin diet group.The cows fed sainfoin diet had a lower(P≤0.038)apparent ruminal biohydrogenation of cis-9-C18:1 and C18:3n-3,compared to the cows fed the control diet.The sainfoin diet group had greater(P≤0.018)C18:3n-3 and cis-9,cis-12-C18:2 proportions in the milk FA profile compared to the control diet group.Transfer efficiencies from feed to milk of C18:2,C18:3n-3 and unsaturated FA were greater(P≤0.0179)for the sainfoin diet.Based on the results,it could be concluded that replacing grass silage by sainfoin silage in dairy cow rations reduces ruminal C18:3n-3 biohydrogenation and improves milk FA profile.
文摘【目的】数量性状是红豆草DUS(Distinctness,Uniformity and Stability)测试指南中易受环境条件影响的性状。为了能够顺利开展红豆草DUS测试工作,需要对红豆草品种数量性状的表达状态进行适宜的分级。【方法】以76个红豆草(Onobrychis viciaefolia Scop.)种质资源为研究对象,对红豆草13个数量性状进行观测,选择合适的分级方法将各数量性状的表达状态进行合理的分级,为红豆草品种DUS测试指南研制提供技术支撑。【结果】13个数量性状的变异范围为6.36%~70.58%;采用最小显著差法,将正态分布的荚果长度分为3级,小叶数、复叶长度、穗长度分为5级,节间长度、荚果宽度分为7级,株高、百粒重分为9级;采用极差法,将非正态分布的分枝数分为7级,开花期、茎直径、叶片长度、叶片宽度分为9级。通过聚类分析可将红豆草资源分为3大类群,类群Ⅰ包含18份材料,占24%;类群Ⅱ包含23份材料,占30%;类群Ⅲ包含35份材料,占46%。【结论】研究初步建立了红豆草种质资源13个数量性状的分级指标,为中国红豆草DUS测试提供了科学依据。