P SAG12 _ IPT gene was introduced into an elite rice (Oryza sativa L. ssp. indica ) restorer line Minghui 63 through Agrobacterium _mediated transformation method. Out of 61 independent transgenic plants ...P SAG12 _ IPT gene was introduced into an elite rice (Oryza sativa L. ssp. indica ) restorer line Minghui 63 through Agrobacterium _mediated transformation method. Out of 61 independent transgenic plants obtained, a few acquired a recognizable phenotype in which leave senescence was delayed to a great degree. The results of field plot test on two homozygous transgenic lines indicated: (1) the stay_green ability of transgenic plants was significantly improved; (2) both the seed_setting rate and the number of panicles per plant of transgenic plants were significantly increased compared with that of the non_transgenic plants of Minghui 63; and (3) the plant height of transgenic plants was significantly reduced.展开更多
文摘P SAG12 _ IPT gene was introduced into an elite rice (Oryza sativa L. ssp. indica ) restorer line Minghui 63 through Agrobacterium _mediated transformation method. Out of 61 independent transgenic plants obtained, a few acquired a recognizable phenotype in which leave senescence was delayed to a great degree. The results of field plot test on two homozygous transgenic lines indicated: (1) the stay_green ability of transgenic plants was significantly improved; (2) both the seed_setting rate and the number of panicles per plant of transgenic plants were significantly increased compared with that of the non_transgenic plants of Minghui 63; and (3) the plant height of transgenic plants was significantly reduced.
