Based on the comprehensive analysis of data from petrology and mineralogy,well logging,seismic surveys,paleontology,and geochemistry,a detailed research was conducted on paleoenvironmental and paleoclimatic conditions...Based on the comprehensive analysis of data from petrology and mineralogy,well logging,seismic surveys,paleontology,and geochemistry,a detailed research was conducted on paleoenvironmental and paleoclimatic conditions,and modeling of the source rocks in the second member of the Eocene Wenchang Formation(Wen 2 Member)in the Northern Shunde Subsag at the southwestern margin of the Pearl River Mouth Basin.The Wen 2 Member hosts excellent,thick lacustrine source rocks with strong longitudinal heterogeneity and an average total organic carbon(TOC)content of over 4.9%.The Wen 2 Member can be divided into three units(I,II,III)from bottom to top.Unit I features excellent source rocks with Type I organic matters(average TOC of 5.9%)primarily sourced from lake organisms;Unit II hosts source rocks dominated by Type II2 organic matters(average TOC of 2.2%),which are originated from mixed sources dominated by terrestrial input.Unit III contains good to excellent source rocks dominated by Type II1 organic matters(average TOC of 4.9%),which are mainly contributed by lake organisms and partially by terrestrial input.Under the background of rapid subsidence and limited source supply during intense rifting period in the Eocene,excellent source rocks were developed in Wen 2 Member in the Northern Shunde Subsag under the coordinated control of warm and humid climate,volcanic activity,and deep-water reducing conditions.During the deposition of Unit I,the warm and humid climate and volcanic activity promoted the proliferation of lake algaes,primarily Granodiscus,resulting in high initial productivity,and deep-water reducing conditions enabled satisfactory preservation of organic matters.These factors jointly controlled the development and occurrence of excellent source rocks.During the deposition of Unit II,a transition from warm to cool and semi-arid paleoclimatic conditions led to a decrease in lake algaes and initial productivity.Additionally,enhanced terrestrial input and shallow-water,weakly oxidizing water conditions caused a significant dilution and decomposition of organic matters,degrading the quality of source rocks.During the deposition of Unit III,when the paleoclimatic conditions are cool and humid,Pediastrum and Botryococcus began to thrive,leading to an increase in productivity.Meanwhile,the reducing environment of semi-deep water facilitated the preservation of excellent source rocks,albeit slightly inferior to those in Unit I.The study results clarify the differential origins and development models of various source rocks in the Shunde Sag,offering valuable guidance for evaluating source rocks and selecting petroleum exploration targets in similar marginal sags.展开更多
Organic-rich mudstones and shales,which hold significant potential for shale oil resources,characterize the first member of the Upper Cretaceous Qingshankou Formation(K_(2)qn~1)in the Sanzhao sag of the Songliao Basin...Organic-rich mudstones and shales,which hold significant potential for shale oil resources,characterize the first member of the Upper Cretaceous Qingshankou Formation(K_(2)qn~1)in the Sanzhao sag of the Songliao Basin,NE China.Focusing on 30 core samples obtained from the first shale oil parameter well,named SYY3 in the study area,we systematically analyzed the composition and stratigraphic distribution of the K_(2)qn~1 heteroatomic compounds using electrospray ionization Fourier transform-ion cyclotron resonance mass spectrometry(ESI FT-ICR MS),to assess their geological relevance to shale oil.The findings indicate that in the negative ion mode,the heteroatomic compounds predominantly consist of N_(1),N_(1)O_(1)-N_(1)O_(8),O_(1)-O_(8),O_(1)S_(1)-O_(6)S_(1);contrastingly,in the positive ion mode,they are primarily composed of N_(1)-N_(2),N_(1)O_(1)-N_(1)O_(4),N_(2)O_(1),O_(1)-O_(4),O_(1)S_(1)-O_(2)S_(1).Heteroatomic compound distributions vary significantly with depth in the negative ion mode,with minor variations in the positive ion mode.These distributions are categorized into three types based on the negative ion ratio((N_(1)+N_(1)O_(x))/O_(x)):TypeⅠ(>1.5),TypeⅡ(0.8-1.5),and TypeⅢ(<0.8);typesⅠandⅡgenerally exhibit a broader range of carbon numbers compared to TypeⅢ.The distribution of double bond equivalent(DBE)values across various sample types exhibits minimal variance,whereas that of carbon numbers shows substantial differences.Variations in heteroatomic compound compositions among the samples might have resulted from vertical sedimentary heterogeneity and differing biotic contributions.TypeⅢsamples show a decrease in total organic carbon(TOC)and free oil content(S_(1))compared to typesⅠandⅡ,but an increased oil saturation index(OSI),indicating a lower content of free oil but a higher proportion of movable oil.The reduced content of N-containing compounds implies lower paleolake productivity during deposition,leading to a reduction in TOC and S_(1).A lower TOC can enhance oil movability due to reduced oil adsorption,and the decreased presence of polar nitrogenous macromolecules with fewer highC-number heteroatomic compounds further promote shale oil movability.Additionally,the negative ion ratios of N1/N1O1and O2/O1 exhibit positive and negative correlations with the values of TOC,S_(1),and extractable organic matter(EOM),respectively,indicating that the salinity and redox conditions of the depositional water body are the primary controlling factors for both organic matter enrichment and shale oil accumulation.展开更多
Alkaline lacustrine shale is highly heterogeneous,and the complex relationship between the organicinorganic porosity network and hydrocarbon occurrence restricts the effectiveness of shale oil exploration and developm...Alkaline lacustrine shale is highly heterogeneous,and the complex relationship between the organicinorganic porosity network and hydrocarbon occurrence restricts the effectiveness of shale oil exploration and development.Herein,we investigated the Fengcheng Formation(P_(1)f)in Mahu Sag.This study integrated geochemistry,Soxhlet extraction,scanning electron microscopy,gas adsorption,and nuclear magnetic resonance T_(1)-T_(2)spectroscopy to elucidate the microscopic oil occurrence mechanisms in shales.Results indicate the presence of felsic shale,dolomitic shale,lime shale,and mixed shale within the P_(1)f.Matrix pores and microfractures associated with inorganic minerals are the predominant pore types in P_(1)f.Adsorbed oil primarily resides on the surfaces of organic matter and clay minerals,while free oil predominantly occupies inorganic pores and microfractures with larger pore sizes.Variations exist in the quantity and distribution of shale oil accumulation across different scales,where free oil and adsorbed oil are governed by dominant pores with diameters exceeding 10 nm and ineffective pores with diameters below 10 nm,respectively.Shale oil occurrence characteristics are influenced by organic matter,pore structure,and mineral composition.Felsic shale exhibits a high abundance of dominant pores,possesses the highest oil content,predominantly harbors free oil within these dominant pores,and demonstrates good mobility.Fluid occurrence in dolomitic shale and lime shale is intricate,with low oil content and a free oil to adsorbed oil ratio of 1:1.Mixed shale exhibits elevated clay mineral content and a scarcity of dominant pores.Moreover,ineffective pores contain increased bound water,resulting in medium oil content and limited mobility predominantly due to adsorption.Presently,shale oil mainly occurs in the dominant pores with a diameter larger than 10 nm in a free state.During the exploration and development of alkaline lacustrine shale oil resources,emphasis should be placed on identifying sweet spots within the felsic shale characterized by dominant pores.展开更多
Based on the experimental results of casting thin section,low temperature nitrogen adsorption,high pressure mercury injection,nuclear magnetic resonance T2 spectrum,contact angle and oil-water interfacial tension,the ...Based on the experimental results of casting thin section,low temperature nitrogen adsorption,high pressure mercury injection,nuclear magnetic resonance T2 spectrum,contact angle and oil-water interfacial tension,the relationship between pore throat structure and crude oil mobility characteristics of full particle sequence reservoirs in the Lower Permian Fengcheng Formation of Mahu Sag,Junggar Basin,are revealed.(1)With the decrease of reservoir particle size,the volume of pores connected by large throats and the volume of large pores show a decreasing trend,and the distribution and peak ranges of throat and pore radius shift to smaller size in an orderly manner.The upper limits of throat radius,porosity and permeability of unconventional reservoirs in Fengcheng Formation are approximately 0.7μm,8%and 0.1×10^(−3)μm^(2),respectively.(2)As the reservoir particle size decreases,the distribution and peak ranges of pores hosting retained oil and movable oil are shifted to a smaller size in an orderly manner.With the increase of driving pressure,the amount of retained and movable oil of the larger particle reservoir samples shows a more obvious trend of decreasing and increasing,respectively.(3)With the increase of throat radius,the driving pressure of reservoir with different particle levels presents three stages,namely rapid decrease,slow decrease and stabilization.The oil driving pressures of various reservoirs and the differences of them decrease with the increase of temperature and obviously decrease with the increase of throat radius.According to the above experimental analysis,it is concluded that the deep shale oil of Fengcheng Formation in Mahu Sag has great potential for production under geological conditions.展开更多
For shale oil reservoirs in the Jimsar Sag of Junggar Basin,the fracturing treatments are challenged by poor prediction accuracy and difficulty in parameter optimization.This paper presents a fracturing parameter inte...For shale oil reservoirs in the Jimsar Sag of Junggar Basin,the fracturing treatments are challenged by poor prediction accuracy and difficulty in parameter optimization.This paper presents a fracturing parameter intelligent optimization technique for shale oil reservoirs and verifies it by field application.A self-governing database capable of automatic capture,storage,calls and analysis is established.With this database,22 geological and engineering variables are selected for correlation analysis.A separated fracturing effect prediction model is proposed,with the fracturing learning curve decomposed into two parts:(1)overall trend,which is predicted by the algorithm combining the convolutional neural network with the characteristics of local connection and parameter sharing and the gated recurrent unit that can solve the gradient disappearance;and(2)local fluctuation,which is predicted by integrating the adaptive boosting algorithm to dynamically adjust the random forest weight.A policy gradient-genetic-particle swarm algorithm is designed,which can adaptively adjust the inertia weights and learning factors in the iterative process,significantly improving the optimization ability of the optimization strategy.The fracturing effect prediction and optimization strategy are combined to realize the intelligent optimization of fracturing parameters.The field application verifies that the proposed technique significantly improves the fracturing effects of oil wells,and it has good practicability.展开更多
Addressing the critical challenges of viscosity loss and barite sag in synthetic-based drilling fluids(SBDFs)under high-temperature,high-pressure(HTHP)conditions,this study innovatively developed a hyperbranched amide...Addressing the critical challenges of viscosity loss and barite sag in synthetic-based drilling fluids(SBDFs)under high-temperature,high-pressure(HTHP)conditions,this study innovatively developed a hyperbranched amide polymer(SS-1)through a unique stepwise polycondensation strategy.By integrating dynamic ionic crosslinking for temperature-responsive rheology and rigid aromatic moieties ensuring thermal stability beyond 260℃,SS-1 achieves a molecular-level breakthrough.Performance evaluations demonstrate that adding merely 2.0 wt% SS-1 significantly enhances key properties of 210℃-aged SBDFs:plastic viscosity rises to 45 mPa⋅s,electrical stability(emulsion voltage)reaches 1426 V,and the sag factor declines to 0.509,outperforming conventional sulfonated polyacrylamide(S-PAM,0.531)by 4.3%.Mechanistic investigations reveal a trifunctional synergistic anti-sag mechanism involving electrostatic adsorption onto barite surfaces,hyperbranched steric hindrance,and colloid-stabilizing network formation.SS-1 exhibits exceptional HTHP stabilization efficacy,substantially surpassing S-PAM,thereby providing an innovative molecular design strategy and scalable solution for next-generation high-performance drilling fluid stabilizers.展开更多
The Shanan sag in the central-western Bohai Bay Basin hosts high-quality Paleogene source rocks within the Shahejie Formation’s third member(E_(2)s_(3)).Despite hydrocarbon indications in Cenozoic strata,no commercia...The Shanan sag in the central-western Bohai Bay Basin hosts high-quality Paleogene source rocks within the Shahejie Formation’s third member(E_(2)s_(3)).Despite hydrocarbon indications in Cenozoic strata,no commercial accumulations have been discovered.An integrated approach combining geochemical analysis,fluid inclusion thermometry,apatite fission-track(AFT)thermochronology,and basin modeling was employed to unravel the paleo-geothermal regime and hydrocarbon generation history of E_(2)s_(3) source rocks.AFT data from the Shahejie Formation’s second member(E_(2)s_(2))reveal a tectonothermal event at 25 Ma that accelerated E_(2)s_(3) maturation.Outside three sub-sag depocenters,current E_(2)s_(2) reservoir temperatures remain below the 25 Ma paleo-geothermal maxima despite subsequent Neogene burial.Hydrocarbon-bearing brine inclusions in E_(2)s_(2) reservoirs exhibit peak homogenization temperatures(Th)at 25 Ma,with minimal high-temperature signals,indicating that E_(2)s_(3) hydrocarbon generation peaked during the Paleogene thermal event,with limited late-stage accumulation.The regional effects of the Dongying Movement necessitate thick Neogene sedimentation to compensate for the 25 Ma paleo-geothermal anomaly.Our findings emphasize targeting Neogene depocenters in petroleum exploration to mitigate the inhibitory effects of high paleo-heat flow on late hydrocarbon generation,thereby enhancing current accumulation potential.展开更多
The study on sand body connectivity and distribution patterns is of great significance for well emplacement and injection-production pattern analysis in the A oilfield of the Weixi?nan Sag currently at a pre-developme...The study on sand body connectivity and distribution patterns is of great significance for well emplacement and injection-production pattern analysis in the A oilfield of the Weixi?nan Sag currently at a pre-development stage.Based on the current drilling data,seismic data,and fault development characteristics,this study investigates the connectivity,geometric morphology,planar distribution,and vertical evolution of composite sand bodies(multi-stage superimposed channel sand bodies)within the fault block using seismic forward and inversion modeling.The El3I oil layer group in the third member of the Liushagang Formation is developed in the fan delta-front sub-facies,which mainly consists of subaqueous distributary channels.The thickness of single-stage subaqueous distributary channel sand bodies ranges from 2 to 6 m,and the width of composite channel sand bodies varies from 50 to 100 m.Under the long-term transgression background,the subaqueous distributary channels in the El3I oil layer group are relatively narrow,forming superimposed and continuous composite channel sand bodies through lateral migration and vertical stacking.The long-term base-level cycles control the width of subaqueous distributary channels,while the mid-term base-level cycles control the thickness of these channels.The subaqueous distributary channels developed during the late stage of mid-term base-level fall are thicker than those formed during the early stage.Accordingly,quantitative relationships between channel thickness and width are established for the early and late stages of mid-term base-level fall,to finely depict the evolution patterns of channel sand body geometry and stacking styles across different stages.These findings provide important guidance for accurately predicting the planar distribution and channel width of composite subaqueous distributary channels at different stages of the mid-term baselevel cycles.展开更多
Based on drilling,mud logging,core,seismic and imaging logging data,this paper studies the identification and evolution process of negative inversion structures in the Carboniferous buried hills in the No.1 and No.2 f...Based on drilling,mud logging,core,seismic and imaging logging data,this paper studies the identification and evolution process of negative inversion structures in the Carboniferous buried hills in the No.1 and No.2 fault zones of Weixinan Sag,Beibu Gulf Basin,China,and reveals the controls of these structures on high-quality reservoirs.The No.2 fault zone develops significant negative inversion structures in the Carboniferous buried hills,as a result of multi-stage transformations of compressive-tensile stress fields in the period from the Late Hercynian to the Himalayan.The Hercynian carbonates laid the material basis for the formation of high-quality reservoirs.The negative inversion structures mainly control the development of high-quality reservoirs in buried hills through:(1)creating large-scale fractures to increase reservoir space and improve oil-gas flow pathways;(2)regulating stratigraphic differential denudation to highlight dominant lithology for later reservoir transformation;(3)shaping the paleogeomorphological highlands to provide favorable conditions for superficial karstification.The negative inversion structures form a high-quality,composite reservoir space with the synergistic existence of superficial dissolution fractures/cavities and burial-enhanced karst systems through the coupling of fracture network creation,formation denudation screening and multi-stage karst transformation.The research results have guided the breakthrough of the first exploratory well with a daily oil production over 1000 m^(3)in carbonate buried-hill reservoir in the Beibu Gulf Basin,and provide referential geological basis for finding more reserves and achieving higher production in the Carboniferous buried hills in the Weixinan Sag.展开更多
The Jurassic tight sandstone oil and gas exploration and development in the eastern Yangxia Sag is a new field.To elucidate the origin,accumulation process and potential of tight oil and gas,the authors have conducted...The Jurassic tight sandstone oil and gas exploration and development in the eastern Yangxia Sag is a new field.To elucidate the origin,accumulation process and potential of tight oil and gas,the authors have conducted comprehensive analyses employing methodologies encompassing source rocks,oil geochemistry,and fluid inclusions.The results show that the abundance of organic matter of Jurassic source rocks is high,and the type of organic matter is ofⅡ-Ⅲand in mature evolution stage.The main source rocks of oil and gas are Huangshanjie Formation and Jurassic coal-bearing source rocks.Ahe Formation developed two stages of hydrocarbon charging,and the period is later than the reservoir densification time.Yangxia Formation oil charged before the reservoir densified,and the late gas charged after the reservoir densified.Hydrocarbon generation intensity of Jurassic source rocks has reached the basic conditions for the formation of tight gas reservoirs.Controlled by the difference of source rocks distribution and accumulation process,tight sandstone oil and gas accumulation conditions are better in the depression direction than in the southeast margin area.This study is of practical importance for expanding the exploration field and selecting favorable areas in the eastern Yangxia sag.展开更多
The Beibu Gulf Basin is an important oil-and gas-bearing basin offshore the China Sea,but the geological reserves of oil and gas are not very high,and there are significant differences between different sags.Previous ...The Beibu Gulf Basin is an important oil-and gas-bearing basin offshore the China Sea,but the geological reserves of oil and gas are not very high,and there are significant differences between different sags.Previous studies have shown that the formation and accumulation of oil and gas are closely related to deep structures,especially the crustal thickness or the relative undulation characteristics between the Moho and basement,but there is a lack of specific evaluation and quantitative standards.In this paper,we first invert the depth of the Moho in the Beibu Gulf Basin using satellite gravity anomalies and calculate the crustal thickness value based on this.Then,we count and analyze the crustal thickness value as well as the stretching factor that characterizes the crustal extension and thinning degree,and find that the NWW-trending contour closure of the 28 km Moho depth or 26 km crustal thickness corresponds significantly to the Weixinan-Fushan sag belt,which has already been proven to have considerable oil and gas discoveries,with a typical feature of crustal thinning to 22 km and the stretching factor reaching 1.1.Finally,we propose a comprehensive evaluation scheme based on the entropy weight method to normalize and evaluate the statistical features of the crust,and take a comprehensive score of Si>0.5 as an evaluation indicator for an oil-gas-rich sag.Based on this research,we believe that the Haizhong sag has considerable exploratory potential,followed by the Maichen sag,while the Leidong and Haitoubei sags have relatively smaller exploratory potential.The research results will help strategic decision-making of the oil and gas exploration in the Beibu Gulf Basin and provide new perspectives for studying the relationship between deep structures and the differential distribution of oil-gas-rich sags.展开更多
Research based on oil accumulation models is essential for exploring the hydrocarbon accumulation theory further.Studies on tight oil accumulation models focused on fan delta depositional systems,and in particular,sys...Research based on oil accumulation models is essential for exploring the hydrocarbon accumulation theory further.Studies on tight oil accumulation models focused on fan delta depositional systems,and in particular,systems involving source-reservoir separated type are scarce.To explore the accumulation model of tight oil in conglomerate,this study focused on the Permian-Triassic tight conglomerate oil in Mahu sag,Junggar Basin,using well drilling,well logging,seismic profiling,oil testing,and laboratory data,and analyzed the formation conditions,formation types,and distribution patterns of conglomerate reservoirs.The results show that,the conglomerate reservoirs are predominantly lithologic reservoirs and partly fault-lithologic reservoirs;there is no water evident at the edge or bottom around the reservoirs.The tight conglomerate layer in the delta plain subfacies of each fan exhibits high clay content and intense diagenesis,and the argillaceous rocks in the pro-fan delta subfacies and shallow lacustrine facies form the sealing and floor conditions.The sandy conglomerate of fan delta front subfacies is the main reservoir body.Additionally,strikeslip faulting in the Indosinian-Himalayan period formed an efficient faulting system for trans-stratal migration with Hercynian-Indosinian inverse faulting.Oil migration is driven by the overpressure caused by hydrocarbon generation from alkali lacustrine source rocks.The distribution of reservoirs is primarily controlled by the large fan bodies,namely the Zhongguai,Baijiantan,Karamay,Huangyangquan,Xiazijie,Xiayan,and Dabasong fans.Each fan body forms a group of reservoirs or oilfields,resulting in a widely distributed pattern,according to which reservoir and sealing constitute one whole body—i.e.,patterns of“one sand and one reservoir,one fan and one field.”This results in a quasi-continuous accumulation model,which includes strong oil charging,efficient faulting transportation,trans-stratal migration,and lithologic trapped accumulation.The proposed model is an important supplement to the existing model of quasi-continuous oil and gas accumulation.Overall,this study enriches unconventional oil and gas accumulation theories.展开更多
Understanding the occurrence state of shale oil is crucial for the effective development of shale oil resources.Although the second member of the Kongdian Formation(Ek2)is a key interval for lacustrine shale oil produ...Understanding the occurrence state of shale oil is crucial for the effective development of shale oil resources.Although the second member of the Kongdian Formation(Ek2)is a key interval for lacustrine shale oil production in the Cangdong Sag,Bohai Bay Basin,the occurrence state and controlling factors of shale oil in this formation remain poorly understood.This study established a multi-step programmed pyrolysis,combined with a light hydrocarbon recovery scheme,to quantitatively characterize the shale oil in different occurrence states.An integrated approach utilizing Rock-Eval pyrolysis,pyrolysis-gas chromatography,and crude oil gas chromatography was employed.Factors influencing the shale oil occurrence state were analyzed from petrology and organic geochemistry perspectives.The study revealed significant variations of shale oil occurrence states within the Ek2,attributed to differences in sedimentary organic matter,mineral compositions,sedimentary structures,and thermal maturity.Felsic laminae are the primary reservoir space for oil in laminated shales,and the frequent interbedding of felsic and organic-rich laminae facilitates the retention of free oil.The contents of free and adsorbed oil are primarily influenced by organic matter content and shale storage capacity,both of which exhibit distinct occurrence patterns.Based on the shale reservoir quality classification using the pyrolysis values of S1-1+S1-2 and(S1-1+S1-2)×100/TOC,the Ek2 shale demonstrates significant exploitation potential,with the first-level reservoirs comprising 66%,second-level reservoirs 11%,and third-level reservoirs 23%.These findings provide new insights into the geological accumulation and production of shale oil.展开更多
Guided by the fundamental principles of the whole petroleum system,the control of tectonism,sedimentation,and diagenesis on hydrocarbon accumulation in a rifted basin is studied using the data of petroleum geology and...Guided by the fundamental principles of the whole petroleum system,the control of tectonism,sedimentation,and diagenesis on hydrocarbon accumulation in a rifted basin is studied using the data of petroleum geology and exploration of the second member of the Paleogene Kongdian Formation(Kong-2 Member)in the Cangdong Sag,Bohai Bay Basin,China.It is clarified that the circle structure and circle effects are the marked features of a continental fault petroliferous basin,and they govern the orderly distribution of conventional and unconventional hydrocarbons in the whole petroleum systems of the rifted basin.Tectonic circle zones control sedimentary circle zones,while sedimentary circle zones and diagenetic circle zones control the spatial distribution of favorable reservoirs,thereby determining the orderly distribution of hydrocarbon accumulations in various circles.A model for the integrated,systematic accumulation of conventional and unconventional hydrocarbons under a multi-circle structure of the whole petroleum system of continental rifted basin has been developed.It reveals that each sag of the rifted basin is an independent whole petroleum system and circle system,which encompasses multiple orderly circles of conventional and unconventional hydrocarbons controlled by the same source kitchen.From the outer circle to the middle circle and then to the inner circle,there is an orderly transition from structural and stratigraphic reservoirs,to lithological and structural-lithological reservoirs,and finally to tight oil/gas and shale oil/gas enrichment zones.The significant feature of the whole petroleum system is the orderly control of hydrocarbons by multi-circle stratigraphic coupling,with the integrated,orderly distribution of conventional and unconventional reserves being the inevitable result of the multi-layered interaction within the whole petroleum system.This concept of multi-circle stratigraphic coupling for the orderly,integrated accumulation of conventional and unconventional hydrocarbons has guided significant breakthroughs in the overall,three-dimensional exploration and shale oil exploration in the Cangdong Sag.展开更多
本研究旨在观察斯氏艾美耳球虫裂殖生殖阶段特异性表面抗原Es-SAG13和Es-SAG14的原核表达产物对兔的免疫保护效果。利用相对荧光定量PCR分析Es-SAGs在未孢子化卵囊时期、孢子化卵囊时期、裂殖生殖时期和配子生殖时期的转录水平,并对裂...本研究旨在观察斯氏艾美耳球虫裂殖生殖阶段特异性表面抗原Es-SAG13和Es-SAG14的原核表达产物对兔的免疫保护效果。利用相对荧光定量PCR分析Es-SAGs在未孢子化卵囊时期、孢子化卵囊时期、裂殖生殖时期和配子生殖时期的转录水平,并对裂殖生殖阶段特异性表达的Es-SAG进行原核表达;然后将40只45日龄无球虫幼兔随机分为4组,分别是空白对照组、攻虫对照组、rEs-SAG13免疫组和rEs-SAG14免疫组,分别经颈部皮下注射1 mL PBS、1 mL PBS、1 mL(100μg·mL^(-1))rEs-SAG13、1 mL(100μg·mL^(-1))rEs-SAG14,首免14 d后,同等剂量加强免疫,二免14 d后,除空白对照外经口感染1×104个斯氏艾美耳球虫孢子化卵囊·只-1,观察各组临床症状并于感染21 d后剖检,并统计各组的卵囊排出量、相对增重、料肉比、肝指数和肝病变记分。结果显示:Es-SAGs在各阶段的转录水平存在差异,其中,SAG13、SAG14属于裂殖生殖阶段特异表达的成员,原核表达的rEs-SAG13和rEs-SAG14均具有良好的反应原性。免疫保护试验表明:感染后攻虫对照组出现兔肝球虫病典型症状,而免疫组症状不明显;rEs-SAG13免疫组和rEs-SAG14免疫组的卵囊减少率分别达82.8%和51.9%;免疫组平均相对增重均显著大于攻虫对照组(P<0.05);rEs-SAG13免疫组与攻虫对照组的肝指数、肝病变记分差异均显著(P<0.05),而rEs-SAG14免疫组与攻虫对照组的肝指数、肝病变记分差异均不明显(P>0.05)。rEs-SAG13和rEs-SAG14均能不同程度地减少增重损失和卵囊排出,减轻肝病变,其中rEs-SAG13免疫保护效果更佳。展开更多
基金Supported by the Research Project of China National Offshore Oil Corporation(SCKY-2023-HN-3)。
文摘Based on the comprehensive analysis of data from petrology and mineralogy,well logging,seismic surveys,paleontology,and geochemistry,a detailed research was conducted on paleoenvironmental and paleoclimatic conditions,and modeling of the source rocks in the second member of the Eocene Wenchang Formation(Wen 2 Member)in the Northern Shunde Subsag at the southwestern margin of the Pearl River Mouth Basin.The Wen 2 Member hosts excellent,thick lacustrine source rocks with strong longitudinal heterogeneity and an average total organic carbon(TOC)content of over 4.9%.The Wen 2 Member can be divided into three units(I,II,III)from bottom to top.Unit I features excellent source rocks with Type I organic matters(average TOC of 5.9%)primarily sourced from lake organisms;Unit II hosts source rocks dominated by Type II2 organic matters(average TOC of 2.2%),which are originated from mixed sources dominated by terrestrial input.Unit III contains good to excellent source rocks dominated by Type II1 organic matters(average TOC of 4.9%),which are mainly contributed by lake organisms and partially by terrestrial input.Under the background of rapid subsidence and limited source supply during intense rifting period in the Eocene,excellent source rocks were developed in Wen 2 Member in the Northern Shunde Subsag under the coordinated control of warm and humid climate,volcanic activity,and deep-water reducing conditions.During the deposition of Unit I,the warm and humid climate and volcanic activity promoted the proliferation of lake algaes,primarily Granodiscus,resulting in high initial productivity,and deep-water reducing conditions enabled satisfactory preservation of organic matters.These factors jointly controlled the development and occurrence of excellent source rocks.During the deposition of Unit II,a transition from warm to cool and semi-arid paleoclimatic conditions led to a decrease in lake algaes and initial productivity.Additionally,enhanced terrestrial input and shallow-water,weakly oxidizing water conditions caused a significant dilution and decomposition of organic matters,degrading the quality of source rocks.During the deposition of Unit III,when the paleoclimatic conditions are cool and humid,Pediastrum and Botryococcus began to thrive,leading to an increase in productivity.Meanwhile,the reducing environment of semi-deep water facilitated the preservation of excellent source rocks,albeit slightly inferior to those in Unit I.The study results clarify the differential origins and development models of various source rocks in the Shunde Sag,offering valuable guidance for evaluating source rocks and selecting petroleum exploration targets in similar marginal sags.
基金jointly funded by the National Natural Science Foundation of China(Grant Nos.42072178 and U2244207)the funding project of Northeast Geological S&T Innovation Center of China Geological Survey(Grant No.QCJJ2022-37)Geological Survey Project of China Geological Survey(Grant Nos.DD20190114,DD20230022,and DD20240045)。
文摘Organic-rich mudstones and shales,which hold significant potential for shale oil resources,characterize the first member of the Upper Cretaceous Qingshankou Formation(K_(2)qn~1)in the Sanzhao sag of the Songliao Basin,NE China.Focusing on 30 core samples obtained from the first shale oil parameter well,named SYY3 in the study area,we systematically analyzed the composition and stratigraphic distribution of the K_(2)qn~1 heteroatomic compounds using electrospray ionization Fourier transform-ion cyclotron resonance mass spectrometry(ESI FT-ICR MS),to assess their geological relevance to shale oil.The findings indicate that in the negative ion mode,the heteroatomic compounds predominantly consist of N_(1),N_(1)O_(1)-N_(1)O_(8),O_(1)-O_(8),O_(1)S_(1)-O_(6)S_(1);contrastingly,in the positive ion mode,they are primarily composed of N_(1)-N_(2),N_(1)O_(1)-N_(1)O_(4),N_(2)O_(1),O_(1)-O_(4),O_(1)S_(1)-O_(2)S_(1).Heteroatomic compound distributions vary significantly with depth in the negative ion mode,with minor variations in the positive ion mode.These distributions are categorized into three types based on the negative ion ratio((N_(1)+N_(1)O_(x))/O_(x)):TypeⅠ(>1.5),TypeⅡ(0.8-1.5),and TypeⅢ(<0.8);typesⅠandⅡgenerally exhibit a broader range of carbon numbers compared to TypeⅢ.The distribution of double bond equivalent(DBE)values across various sample types exhibits minimal variance,whereas that of carbon numbers shows substantial differences.Variations in heteroatomic compound compositions among the samples might have resulted from vertical sedimentary heterogeneity and differing biotic contributions.TypeⅢsamples show a decrease in total organic carbon(TOC)and free oil content(S_(1))compared to typesⅠandⅡ,but an increased oil saturation index(OSI),indicating a lower content of free oil but a higher proportion of movable oil.The reduced content of N-containing compounds implies lower paleolake productivity during deposition,leading to a reduction in TOC and S_(1).A lower TOC can enhance oil movability due to reduced oil adsorption,and the decreased presence of polar nitrogenous macromolecules with fewer highC-number heteroatomic compounds further promote shale oil movability.Additionally,the negative ion ratios of N1/N1O1and O2/O1 exhibit positive and negative correlations with the values of TOC,S_(1),and extractable organic matter(EOM),respectively,indicating that the salinity and redox conditions of the depositional water body are the primary controlling factors for both organic matter enrichment and shale oil accumulation.
基金financially supported by the State Key Laboratory of Shale Oil and Gas Enrichment Mechanisms and Efficient Development(33550000-22-ZC0613-0006)National Natural Science Foundation of China(42202133)+2 种基金CNPC Innovation Fund(2022DQ02-0106)Strategic Cooperation Technology Projects of the CNPC and CUPB(ZLZX2020-01-05)Key Laboratory of Tectonics and Petroleum Resources(China University of Geosciences),Ministry of Education,China(TPR-2023-05)。
文摘Alkaline lacustrine shale is highly heterogeneous,and the complex relationship between the organicinorganic porosity network and hydrocarbon occurrence restricts the effectiveness of shale oil exploration and development.Herein,we investigated the Fengcheng Formation(P_(1)f)in Mahu Sag.This study integrated geochemistry,Soxhlet extraction,scanning electron microscopy,gas adsorption,and nuclear magnetic resonance T_(1)-T_(2)spectroscopy to elucidate the microscopic oil occurrence mechanisms in shales.Results indicate the presence of felsic shale,dolomitic shale,lime shale,and mixed shale within the P_(1)f.Matrix pores and microfractures associated with inorganic minerals are the predominant pore types in P_(1)f.Adsorbed oil primarily resides on the surfaces of organic matter and clay minerals,while free oil predominantly occupies inorganic pores and microfractures with larger pore sizes.Variations exist in the quantity and distribution of shale oil accumulation across different scales,where free oil and adsorbed oil are governed by dominant pores with diameters exceeding 10 nm and ineffective pores with diameters below 10 nm,respectively.Shale oil occurrence characteristics are influenced by organic matter,pore structure,and mineral composition.Felsic shale exhibits a high abundance of dominant pores,possesses the highest oil content,predominantly harbors free oil within these dominant pores,and demonstrates good mobility.Fluid occurrence in dolomitic shale and lime shale is intricate,with low oil content and a free oil to adsorbed oil ratio of 1:1.Mixed shale exhibits elevated clay mineral content and a scarcity of dominant pores.Moreover,ineffective pores contain increased bound water,resulting in medium oil content and limited mobility predominantly due to adsorption.Presently,shale oil mainly occurs in the dominant pores with a diameter larger than 10 nm in a free state.During the exploration and development of alkaline lacustrine shale oil resources,emphasis should be placed on identifying sweet spots within the felsic shale characterized by dominant pores.
基金Supported by Leading Talent Program of Autonomous Region(2022TSYCLJ0070)PetroChina Prospective and Basic Technological Project(2021DJ0108)Natural Science Foundation for Outstanding Young People in Shandong Province(ZR2022YQ30).
文摘Based on the experimental results of casting thin section,low temperature nitrogen adsorption,high pressure mercury injection,nuclear magnetic resonance T2 spectrum,contact angle and oil-water interfacial tension,the relationship between pore throat structure and crude oil mobility characteristics of full particle sequence reservoirs in the Lower Permian Fengcheng Formation of Mahu Sag,Junggar Basin,are revealed.(1)With the decrease of reservoir particle size,the volume of pores connected by large throats and the volume of large pores show a decreasing trend,and the distribution and peak ranges of throat and pore radius shift to smaller size in an orderly manner.The upper limits of throat radius,porosity and permeability of unconventional reservoirs in Fengcheng Formation are approximately 0.7μm,8%and 0.1×10^(−3)μm^(2),respectively.(2)As the reservoir particle size decreases,the distribution and peak ranges of pores hosting retained oil and movable oil are shifted to a smaller size in an orderly manner.With the increase of driving pressure,the amount of retained and movable oil of the larger particle reservoir samples shows a more obvious trend of decreasing and increasing,respectively.(3)With the increase of throat radius,the driving pressure of reservoir with different particle levels presents three stages,namely rapid decrease,slow decrease and stabilization.The oil driving pressures of various reservoirs and the differences of them decrease with the increase of temperature and obviously decrease with the increase of throat radius.According to the above experimental analysis,it is concluded that the deep shale oil of Fengcheng Formation in Mahu Sag has great potential for production under geological conditions.
基金Supported by the National Science and Technology Major Project(2017ZX05009-005-003)National Natural Science Grant Fund for Surface Project(52174045)+1 种基金Chinese Academy of Engineering Strategic Consulting Project(2018-XZ-09)China National Petroleum Corporation(CNPC)-China University of Petroleum(Beijing)Special Project for Strategic Cooperation in Science and Technology(ZLZX2020-01)。
文摘For shale oil reservoirs in the Jimsar Sag of Junggar Basin,the fracturing treatments are challenged by poor prediction accuracy and difficulty in parameter optimization.This paper presents a fracturing parameter intelligent optimization technique for shale oil reservoirs and verifies it by field application.A self-governing database capable of automatic capture,storage,calls and analysis is established.With this database,22 geological and engineering variables are selected for correlation analysis.A separated fracturing effect prediction model is proposed,with the fracturing learning curve decomposed into two parts:(1)overall trend,which is predicted by the algorithm combining the convolutional neural network with the characteristics of local connection and parameter sharing and the gated recurrent unit that can solve the gradient disappearance;and(2)local fluctuation,which is predicted by integrating the adaptive boosting algorithm to dynamically adjust the random forest weight.A policy gradient-genetic-particle swarm algorithm is designed,which can adaptively adjust the inertia weights and learning factors in the iterative process,significantly improving the optimization ability of the optimization strategy.The fracturing effect prediction and optimization strategy are combined to realize the intelligent optimization of fracturing parameters.The field application verifies that the proposed technique significantly improves the fracturing effects of oil wells,and it has good practicability.
基金the National Natural Science Foundation(41802169)Key Laboratory of Polar Geology and Marine Mineral Resources Foudation(HNPY-202506)for supporting this study.
文摘The Shanan sag in the central-western Bohai Bay Basin hosts high-quality Paleogene source rocks within the Shahejie Formation’s third member(E_(2)s_(3)).Despite hydrocarbon indications in Cenozoic strata,no commercial accumulations have been discovered.An integrated approach combining geochemical analysis,fluid inclusion thermometry,apatite fission-track(AFT)thermochronology,and basin modeling was employed to unravel the paleo-geothermal regime and hydrocarbon generation history of E_(2)s_(3) source rocks.AFT data from the Shahejie Formation’s second member(E_(2)s_(2))reveal a tectonothermal event at 25 Ma that accelerated E_(2)s_(3) maturation.Outside three sub-sag depocenters,current E_(2)s_(2) reservoir temperatures remain below the 25 Ma paleo-geothermal maxima despite subsequent Neogene burial.Hydrocarbon-bearing brine inclusions in E_(2)s_(2) reservoirs exhibit peak homogenization temperatures(Th)at 25 Ma,with minimal high-temperature signals,indicating that E_(2)s_(3) hydrocarbon generation peaked during the Paleogene thermal event,with limited late-stage accumulation.The regional effects of the Dongying Movement necessitate thick Neogene sedimentation to compensate for the 25 Ma paleo-geothermal anomaly.Our findings emphasize targeting Neogene depocenters in petroleum exploration to mitigate the inhibitory effects of high paleo-heat flow on late hydrocarbon generation,thereby enhancing current accumulation potential.
文摘The study on sand body connectivity and distribution patterns is of great significance for well emplacement and injection-production pattern analysis in the A oilfield of the Weixi?nan Sag currently at a pre-development stage.Based on the current drilling data,seismic data,and fault development characteristics,this study investigates the connectivity,geometric morphology,planar distribution,and vertical evolution of composite sand bodies(multi-stage superimposed channel sand bodies)within the fault block using seismic forward and inversion modeling.The El3I oil layer group in the third member of the Liushagang Formation is developed in the fan delta-front sub-facies,which mainly consists of subaqueous distributary channels.The thickness of single-stage subaqueous distributary channel sand bodies ranges from 2 to 6 m,and the width of composite channel sand bodies varies from 50 to 100 m.Under the long-term transgression background,the subaqueous distributary channels in the El3I oil layer group are relatively narrow,forming superimposed and continuous composite channel sand bodies through lateral migration and vertical stacking.The long-term base-level cycles control the width of subaqueous distributary channels,while the mid-term base-level cycles control the thickness of these channels.The subaqueous distributary channels developed during the late stage of mid-term base-level fall are thicker than those formed during the early stage.Accordingly,quantitative relationships between channel thickness and width are established for the early and late stages of mid-term base-level fall,to finely depict the evolution patterns of channel sand body geometry and stacking styles across different stages.These findings provide important guidance for accurately predicting the planar distribution and channel width of composite subaqueous distributary channels at different stages of the mid-term baselevel cycles.
基金Supported by the Hainan Provincial Science and Technology Special Project(ZDYF2025GXJS013)CNOOC Zhanjiang Branch Project(CCL2023ZJFN0540).
文摘Based on drilling,mud logging,core,seismic and imaging logging data,this paper studies the identification and evolution process of negative inversion structures in the Carboniferous buried hills in the No.1 and No.2 fault zones of Weixinan Sag,Beibu Gulf Basin,China,and reveals the controls of these structures on high-quality reservoirs.The No.2 fault zone develops significant negative inversion structures in the Carboniferous buried hills,as a result of multi-stage transformations of compressive-tensile stress fields in the period from the Late Hercynian to the Himalayan.The Hercynian carbonates laid the material basis for the formation of high-quality reservoirs.The negative inversion structures mainly control the development of high-quality reservoirs in buried hills through:(1)creating large-scale fractures to increase reservoir space and improve oil-gas flow pathways;(2)regulating stratigraphic differential denudation to highlight dominant lithology for later reservoir transformation;(3)shaping the paleogeomorphological highlands to provide favorable conditions for superficial karstification.The negative inversion structures form a high-quality,composite reservoir space with the synergistic existence of superficial dissolution fractures/cavities and burial-enhanced karst systems through the coupling of fracture network creation,formation denudation screening and multi-stage karst transformation.The research results have guided the breakthrough of the first exploratory well with a daily oil production over 1000 m^(3)in carbonate buried-hill reservoir in the Beibu Gulf Basin,and provide referential geological basis for finding more reserves and achieving higher production in the Carboniferous buried hills in the Weixinan Sag.
基金supported by Joint Fund Project of National Natural Science Foundation(No.U22B6002)CNPC Scientific Research and Technology Development Project(No.2023ZZ14YJ02).
文摘The Jurassic tight sandstone oil and gas exploration and development in the eastern Yangxia Sag is a new field.To elucidate the origin,accumulation process and potential of tight oil and gas,the authors have conducted comprehensive analyses employing methodologies encompassing source rocks,oil geochemistry,and fluid inclusions.The results show that the abundance of organic matter of Jurassic source rocks is high,and the type of organic matter is ofⅡ-Ⅲand in mature evolution stage.The main source rocks of oil and gas are Huangshanjie Formation and Jurassic coal-bearing source rocks.Ahe Formation developed two stages of hydrocarbon charging,and the period is later than the reservoir densification time.Yangxia Formation oil charged before the reservoir densified,and the late gas charged after the reservoir densified.Hydrocarbon generation intensity of Jurassic source rocks has reached the basic conditions for the formation of tight gas reservoirs.Controlled by the difference of source rocks distribution and accumulation process,tight sandstone oil and gas accumulation conditions are better in the depression direction than in the southeast margin area.This study is of practical importance for expanding the exploration field and selecting favorable areas in the eastern Yangxia sag.
基金The Scientific and Technological Project of China National Offshore Oil Corporation(CNOOC)Research Institute Co.,Ltd.under contract No.CCL2021RCPS0167KQNthe Open Fund Project for the year 2022 of National Engineering Research Center of Offshore Oil and Gas Exploration under contract No.CCL2022RCPS0794RQN.
文摘The Beibu Gulf Basin is an important oil-and gas-bearing basin offshore the China Sea,but the geological reserves of oil and gas are not very high,and there are significant differences between different sags.Previous studies have shown that the formation and accumulation of oil and gas are closely related to deep structures,especially the crustal thickness or the relative undulation characteristics between the Moho and basement,but there is a lack of specific evaluation and quantitative standards.In this paper,we first invert the depth of the Moho in the Beibu Gulf Basin using satellite gravity anomalies and calculate the crustal thickness value based on this.Then,we count and analyze the crustal thickness value as well as the stretching factor that characterizes the crustal extension and thinning degree,and find that the NWW-trending contour closure of the 28 km Moho depth or 26 km crustal thickness corresponds significantly to the Weixinan-Fushan sag belt,which has already been proven to have considerable oil and gas discoveries,with a typical feature of crustal thinning to 22 km and the stretching factor reaching 1.1.Finally,we propose a comprehensive evaluation scheme based on the entropy weight method to normalize and evaluate the statistical features of the crust,and take a comprehensive score of Si>0.5 as an evaluation indicator for an oil-gas-rich sag.Based on this research,we believe that the Haizhong sag has considerable exploratory potential,followed by the Maichen sag,while the Leidong and Haitoubei sags have relatively smaller exploratory potential.The research results will help strategic decision-making of the oil and gas exploration in the Beibu Gulf Basin and provide new perspectives for studying the relationship between deep structures and the differential distribution of oil-gas-rich sags.
基金the National Science and Technology Major Project of China for their support。
文摘Research based on oil accumulation models is essential for exploring the hydrocarbon accumulation theory further.Studies on tight oil accumulation models focused on fan delta depositional systems,and in particular,systems involving source-reservoir separated type are scarce.To explore the accumulation model of tight oil in conglomerate,this study focused on the Permian-Triassic tight conglomerate oil in Mahu sag,Junggar Basin,using well drilling,well logging,seismic profiling,oil testing,and laboratory data,and analyzed the formation conditions,formation types,and distribution patterns of conglomerate reservoirs.The results show that,the conglomerate reservoirs are predominantly lithologic reservoirs and partly fault-lithologic reservoirs;there is no water evident at the edge or bottom around the reservoirs.The tight conglomerate layer in the delta plain subfacies of each fan exhibits high clay content and intense diagenesis,and the argillaceous rocks in the pro-fan delta subfacies and shallow lacustrine facies form the sealing and floor conditions.The sandy conglomerate of fan delta front subfacies is the main reservoir body.Additionally,strikeslip faulting in the Indosinian-Himalayan period formed an efficient faulting system for trans-stratal migration with Hercynian-Indosinian inverse faulting.Oil migration is driven by the overpressure caused by hydrocarbon generation from alkali lacustrine source rocks.The distribution of reservoirs is primarily controlled by the large fan bodies,namely the Zhongguai,Baijiantan,Karamay,Huangyangquan,Xiazijie,Xiayan,and Dabasong fans.Each fan body forms a group of reservoirs or oilfields,resulting in a widely distributed pattern,according to which reservoir and sealing constitute one whole body—i.e.,patterns of“one sand and one reservoir,one fan and one field.”This results in a quasi-continuous accumulation model,which includes strong oil charging,efficient faulting transportation,trans-stratal migration,and lithologic trapped accumulation.The proposed model is an important supplement to the existing model of quasi-continuous oil and gas accumulation.Overall,this study enriches unconventional oil and gas accumulation theories.
基金supported by the National Natural Science Foundation of China(No.41830431)the Shandong Provincial Key Research and Development Program(No.2020ZLYS08).
文摘Understanding the occurrence state of shale oil is crucial for the effective development of shale oil resources.Although the second member of the Kongdian Formation(Ek2)is a key interval for lacustrine shale oil production in the Cangdong Sag,Bohai Bay Basin,the occurrence state and controlling factors of shale oil in this formation remain poorly understood.This study established a multi-step programmed pyrolysis,combined with a light hydrocarbon recovery scheme,to quantitatively characterize the shale oil in different occurrence states.An integrated approach utilizing Rock-Eval pyrolysis,pyrolysis-gas chromatography,and crude oil gas chromatography was employed.Factors influencing the shale oil occurrence state were analyzed from petrology and organic geochemistry perspectives.The study revealed significant variations of shale oil occurrence states within the Ek2,attributed to differences in sedimentary organic matter,mineral compositions,sedimentary structures,and thermal maturity.Felsic laminae are the primary reservoir space for oil in laminated shales,and the frequent interbedding of felsic and organic-rich laminae facilitates the retention of free oil.The contents of free and adsorbed oil are primarily influenced by organic matter content and shale storage capacity,both of which exhibit distinct occurrence patterns.Based on the shale reservoir quality classification using the pyrolysis values of S1-1+S1-2 and(S1-1+S1-2)×100/TOC,the Ek2 shale demonstrates significant exploitation potential,with the first-level reservoirs comprising 66%,second-level reservoirs 11%,and third-level reservoirs 23%.These findings provide new insights into the geological accumulation and production of shale oil.
基金Supported by the National Science and Technology Major Project of China(2024ZD1400101)China National Key Research and Development Project(2022YFF0801204)Major Science and Technology Project of CNPC(2023ZZ15YJ01,2021DJ0702)。
文摘Guided by the fundamental principles of the whole petroleum system,the control of tectonism,sedimentation,and diagenesis on hydrocarbon accumulation in a rifted basin is studied using the data of petroleum geology and exploration of the second member of the Paleogene Kongdian Formation(Kong-2 Member)in the Cangdong Sag,Bohai Bay Basin,China.It is clarified that the circle structure and circle effects are the marked features of a continental fault petroliferous basin,and they govern the orderly distribution of conventional and unconventional hydrocarbons in the whole petroleum systems of the rifted basin.Tectonic circle zones control sedimentary circle zones,while sedimentary circle zones and diagenetic circle zones control the spatial distribution of favorable reservoirs,thereby determining the orderly distribution of hydrocarbon accumulations in various circles.A model for the integrated,systematic accumulation of conventional and unconventional hydrocarbons under a multi-circle structure of the whole petroleum system of continental rifted basin has been developed.It reveals that each sag of the rifted basin is an independent whole petroleum system and circle system,which encompasses multiple orderly circles of conventional and unconventional hydrocarbons controlled by the same source kitchen.From the outer circle to the middle circle and then to the inner circle,there is an orderly transition from structural and stratigraphic reservoirs,to lithological and structural-lithological reservoirs,and finally to tight oil/gas and shale oil/gas enrichment zones.The significant feature of the whole petroleum system is the orderly control of hydrocarbons by multi-circle stratigraphic coupling,with the integrated,orderly distribution of conventional and unconventional reserves being the inevitable result of the multi-layered interaction within the whole petroleum system.This concept of multi-circle stratigraphic coupling for the orderly,integrated accumulation of conventional and unconventional hydrocarbons has guided significant breakthroughs in the overall,three-dimensional exploration and shale oil exploration in the Cangdong Sag.
文摘本研究旨在观察斯氏艾美耳球虫裂殖生殖阶段特异性表面抗原Es-SAG13和Es-SAG14的原核表达产物对兔的免疫保护效果。利用相对荧光定量PCR分析Es-SAGs在未孢子化卵囊时期、孢子化卵囊时期、裂殖生殖时期和配子生殖时期的转录水平,并对裂殖生殖阶段特异性表达的Es-SAG进行原核表达;然后将40只45日龄无球虫幼兔随机分为4组,分别是空白对照组、攻虫对照组、rEs-SAG13免疫组和rEs-SAG14免疫组,分别经颈部皮下注射1 mL PBS、1 mL PBS、1 mL(100μg·mL^(-1))rEs-SAG13、1 mL(100μg·mL^(-1))rEs-SAG14,首免14 d后,同等剂量加强免疫,二免14 d后,除空白对照外经口感染1×104个斯氏艾美耳球虫孢子化卵囊·只-1,观察各组临床症状并于感染21 d后剖检,并统计各组的卵囊排出量、相对增重、料肉比、肝指数和肝病变记分。结果显示:Es-SAGs在各阶段的转录水平存在差异,其中,SAG13、SAG14属于裂殖生殖阶段特异表达的成员,原核表达的rEs-SAG13和rEs-SAG14均具有良好的反应原性。免疫保护试验表明:感染后攻虫对照组出现兔肝球虫病典型症状,而免疫组症状不明显;rEs-SAG13免疫组和rEs-SAG14免疫组的卵囊减少率分别达82.8%和51.9%;免疫组平均相对增重均显著大于攻虫对照组(P<0.05);rEs-SAG13免疫组与攻虫对照组的肝指数、肝病变记分差异均显著(P<0.05),而rEs-SAG14免疫组与攻虫对照组的肝指数、肝病变记分差异均不明显(P>0.05)。rEs-SAG13和rEs-SAG14均能不同程度地减少增重损失和卵囊排出,减轻肝病变,其中rEs-SAG13免疫保护效果更佳。
