Native promoters that can drive high and stable transgene expression are important tools for modifying plant traits.Although several such promoters have been reported in soybean(Glycine max),few of them function at mu...Native promoters that can drive high and stable transgene expression are important tools for modifying plant traits.Although several such promoters have been reported in soybean(Glycine max),few of them function at multiple growth and development stages and during nodule development.Here,we report that the promoters of 40S RIBOSOMAL PROTEIN SMALL SUBUNIT S28(RPS28)and EUKARYOTIC TRANSLATION INITIATION FACTOR 1(EIF1)are ideal for high expression of transgene.Through bioinformatic analysis,we determined that RPS28 and EIF1 were highly expressed during soybean growth and development,nodule development,and various biotic and abiotic stresses.Fusion of both RPS28 and EIF1 promoters,with or without their first intron,with the reporter geneβ-GLUCURONIDASE(uidA)in transgenic soybean,resulted in high GUS activity in seedlings,seeds,and nodules.Fluorimetric GUS assays showed that the RPS28 promoter and the EIF1 promoter yielded high expression,comparable to the soybean Ubiquitin(GmUbi)promoter.RPS28 and EIF1 promoters were also highly expressed in Arabidopsis thaliana and Nicotiana benthamiana.Our results indicate the potential of RPS28 and EIF1 promoters to facilitate future genetic engineering and breeding to improve the quality and yield of soybean,as well as in a wide variety of other plant species.展开更多
Eucotylidae is a family of microphalloid digeneans parasitising as adults in the urinary system of birds.There are few molecular data on members of this family,and phylogenetic relationships of its genera and species ...Eucotylidae is a family of microphalloid digeneans parasitising as adults in the urinary system of birds.There are few molecular data on members of this family,and phylogenetic relationships of its genera and species are still unclear.Here we present new morphological and molecular data on a eucotylid species Tanaisia atra(Nezlobinsky,1926).Specimens of T.atra were found in Eurasian Coot Fulica atra Linnaeus,1758 in European and Asian parts of Russia and examined using optical and SEM microscopy and molecular techniques.We report the presence of a well-developed cirrus sac and different types of tegumental scales in this species for the first time.All molecular markers used in our study(ITS2 region,28S rRNA and cox1gene)indicate that T.atra isolates from European and Asian parts of Russia are conspecific.In phylograms based on ITS2 region and 28S rRNA gene sequences,T.atra clustered with other Tanaisia spp.展开更多
基金supported by National Natural Science Foundationof China(Grant No.31870257 and U21A20181 to X.W,Grant no.32170728 to H.W.)the National Key Research,and Development,Program(Grant No.2018YFE0112100 to X.W.)+1 种基金Excellent Youth Foundation of Henan Province(Grant No.222300420025 to H.W.)the 111 Project of China(Grant No.D16014).
文摘Native promoters that can drive high and stable transgene expression are important tools for modifying plant traits.Although several such promoters have been reported in soybean(Glycine max),few of them function at multiple growth and development stages and during nodule development.Here,we report that the promoters of 40S RIBOSOMAL PROTEIN SMALL SUBUNIT S28(RPS28)and EUKARYOTIC TRANSLATION INITIATION FACTOR 1(EIF1)are ideal for high expression of transgene.Through bioinformatic analysis,we determined that RPS28 and EIF1 were highly expressed during soybean growth and development,nodule development,and various biotic and abiotic stresses.Fusion of both RPS28 and EIF1 promoters,with or without their first intron,with the reporter geneβ-GLUCURONIDASE(uidA)in transgenic soybean,resulted in high GUS activity in seedlings,seeds,and nodules.Fluorimetric GUS assays showed that the RPS28 promoter and the EIF1 promoter yielded high expression,comparable to the soybean Ubiquitin(GmUbi)promoter.RPS28 and EIF1 promoters were also highly expressed in Arabidopsis thaliana and Nicotiana benthamiana.Our results indicate the potential of RPS28 and EIF1 promoters to facilitate future genetic engineering and breeding to improve the quality and yield of soybean,as well as in a wide variety of other plant species.
基金supported by the Russian Ministry of Science and Higher Education FFER-2024-0027。
文摘Eucotylidae is a family of microphalloid digeneans parasitising as adults in the urinary system of birds.There are few molecular data on members of this family,and phylogenetic relationships of its genera and species are still unclear.Here we present new morphological and molecular data on a eucotylid species Tanaisia atra(Nezlobinsky,1926).Specimens of T.atra were found in Eurasian Coot Fulica atra Linnaeus,1758 in European and Asian parts of Russia and examined using optical and SEM microscopy and molecular techniques.We report the presence of a well-developed cirrus sac and different types of tegumental scales in this species for the first time.All molecular markers used in our study(ITS2 region,28S rRNA and cox1gene)indicate that T.atra isolates from European and Asian parts of Russia are conspecific.In phylograms based on ITS2 region and 28S rRNA gene sequences,T.atra clustered with other Tanaisia spp.
