Chilling stress is a production constraint of tomato,a tropical origin,chilling-sensitive horticultural crop.The development of chilling tolerant tomato thus has significant potential to impact tomato production.Gluta...Chilling stress is a production constraint of tomato,a tropical origin,chilling-sensitive horticultural crop.The development of chilling tolerant tomato thus has significant potential to impact tomato production.Glutaredoxins(GRXs)are ubiquitous oxidoreductases,which utilize the reducing power of glutathione to reduce disulfide bonds of substrate proteins and maintain cellular redox homeostasis.Here,we report that tomato expressing Arabidopsis GRX gene AtGRXS17 conferred tolerance to chilling stress without adverse effects on growth and development.AtGRXS17-expressing tomato plants displayed lower ion leakage,higher maximal photochemical efficiency of photosystem II(Fv/Fm)and increased accumulation of soluble sugar compared with wild-type plants after the chilling stress challenge.Furthermore,chilling tolerance was correlated with increased antioxidant enzyme activities and reduced H2O2 accumulation.At the same time,temporal expression patterns of the endogenous C-repeat/DRE-binding factor 1(SlCBF1)and CBF mediated-cold regulated genes were not altered in AtGRXS17-expressing plants when compared with wild-type plants,and proline concentrations remained unchanged relative to wild-type plants under chilling stress.Green fluorescent protein-AtGRXS17 fusion proteins,which were initially localized in the cytoplasm,migrated into the nucleus during chilling stress,reflecting a possible role of AtGRXS17 in nuclear signaling of chilling stress responses.Together,our findings demonstrate that genetically engineered tomato plants expressing AtGRXS17 can enhance chilling tolerance and suggest a genetic engineering strategy to improve chilling tolerance without yield penalty across different crop species.展开更多
红17S是福建省南平市农业科学研究所以广占63S为母本与南平当地优质特色红米美人红杂交,后又与籼型两系不育系K12S复交,经福建建阳及海南三亚等地充分利用自然生态条件,4 a 8代培育成的特色红米温敏核不育系,2022年通过福建省农作物品...红17S是福建省南平市农业科学研究所以广占63S为母本与南平当地优质特色红米美人红杂交,后又与籼型两系不育系K12S复交,经福建建阳及海南三亚等地充分利用自然生态条件,4 a 8代培育成的特色红米温敏核不育系,2022年通过福建省农作物品种审定。总结了红17S的选育过程、配组应用及配套繁殖、制种技术。展开更多
基金We thank Dr.Barbara Valent for supplying pBV579(35S::mCherry::NLS::Tnos)This research was supported by the Kansas State University AES project NAHF381121(to S.H.P.,Q.W.,S.A.S.)National Science Foundation award IOS-1258028(F.F.W.,Y.H.,J.P.).
文摘Chilling stress is a production constraint of tomato,a tropical origin,chilling-sensitive horticultural crop.The development of chilling tolerant tomato thus has significant potential to impact tomato production.Glutaredoxins(GRXs)are ubiquitous oxidoreductases,which utilize the reducing power of glutathione to reduce disulfide bonds of substrate proteins and maintain cellular redox homeostasis.Here,we report that tomato expressing Arabidopsis GRX gene AtGRXS17 conferred tolerance to chilling stress without adverse effects on growth and development.AtGRXS17-expressing tomato plants displayed lower ion leakage,higher maximal photochemical efficiency of photosystem II(Fv/Fm)and increased accumulation of soluble sugar compared with wild-type plants after the chilling stress challenge.Furthermore,chilling tolerance was correlated with increased antioxidant enzyme activities and reduced H2O2 accumulation.At the same time,temporal expression patterns of the endogenous C-repeat/DRE-binding factor 1(SlCBF1)and CBF mediated-cold regulated genes were not altered in AtGRXS17-expressing plants when compared with wild-type plants,and proline concentrations remained unchanged relative to wild-type plants under chilling stress.Green fluorescent protein-AtGRXS17 fusion proteins,which were initially localized in the cytoplasm,migrated into the nucleus during chilling stress,reflecting a possible role of AtGRXS17 in nuclear signaling of chilling stress responses.Together,our findings demonstrate that genetically engineered tomato plants expressing AtGRXS17 can enhance chilling tolerance and suggest a genetic engineering strategy to improve chilling tolerance without yield penalty across different crop species.
文摘红17S是福建省南平市农业科学研究所以广占63S为母本与南平当地优质特色红米美人红杂交,后又与籼型两系不育系K12S复交,经福建建阳及海南三亚等地充分利用自然生态条件,4 a 8代培育成的特色红米温敏核不育系,2022年通过福建省农作物品种审定。总结了红17S的选育过程、配组应用及配套繁殖、制种技术。
