针对深井与超深井高温重载工况下S13Cr油管表面裂纹易引发失效的问题,基于失效评估图开展安全评定研究。通过单轴拉伸与热膨胀试验获取40℃、80℃、120℃、160℃下S13Cr的力学参数与热膨胀系数,得到了S13Cr的本构参数;建立Φ88.9 mm S1...针对深井与超深井高温重载工况下S13Cr油管表面裂纹易引发失效的问题,基于失效评估图开展安全评定研究。通过单轴拉伸与热膨胀试验获取40℃、80℃、120℃、160℃下S13Cr的力学参数与热膨胀系数,得到了S13Cr的本构参数;建立Φ88.9 mm S13Cr油管三维有限元模型,计算环向裂纹的应力强度因子与塑性极限载荷;基于R6规范确定失效评估曲线,通过断裂比(K_(r))与载荷比(L_(r))进行安全评定。结果表明,800 kN轴向拉力与30 MPa压差工况下,裂纹深度a/t=0.3~0.7、形状比a/c=0.8~1.2的S13Cr油管评定点均位于FAD图安全区。展开更多
目的克隆人核糖体蛋白S13(ribosomal protein S13,RPS13)cDNA全长,构建原核表达质粒,诱导表达、纯化RPS13蛋白并制备其多克隆抗体。方法应用RT-PCR方法从胃癌多药耐药细胞SGC7901/VCR中扩增出RPS13的cDNA全长,利用DNA重组技术构建重组...目的克隆人核糖体蛋白S13(ribosomal protein S13,RPS13)cDNA全长,构建原核表达质粒,诱导表达、纯化RPS13蛋白并制备其多克隆抗体。方法应用RT-PCR方法从胃癌多药耐药细胞SGC7901/VCR中扩增出RPS13的cDNA全长,利用DNA重组技术构建重组原核表达载体pET-28a(+)-RPS13,双酶切及测序鉴定。将重组载体转化入大肠杆菌BL21中,筛选抗性克隆并经DNA测序证实。IPTG诱导融合蛋白的表达,利用镍离子亲和层析柱提纯融合蛋白,经SDS-PAGE和Western blot鉴定。用纯化的融合蛋白His-RPS13免疫BALB/c小鼠,ELISA法测定抗体效价,饱和硫酸铵沉淀法纯化抗体,Western blot检测抗体活性。结果RT-PCR扩增片段与预计目的基因片段大小一致;双酶切鉴定表明,重组表达载体pET-28a(+)-RPS13构建成功,DNA测序结果显示所获基因序列与GenBank中收录完全相同。IPTG诱导3h后,经SDS-PAGE检测显示在Mr19000处出现一新生条带,与预计的融合白大小一致;利用抗His标签的抗体进行Western blot,结果证实融合蛋白表达成功。其免疫血清经Western blot证实可特异性的识别蛋白RPS13。结论成功地获得了RPS13的编码基因序列,并获得了纯化的RPS13蛋白质,为制备RPS13的单克隆抗体、进一步研究RPS13在肿瘤中的作用奠定了基础。展开更多
核糖体蛋白S13(Ribosomal protein S13,RPS13)属于核糖体蛋白家族,在核糖体中的生物学功能主要是维持核糖体大小亚基之间的动态连接。从蛇床子[Cnidium monnieri(L.)Cuss.]的根、茎、叶组织中提取总RNA,通过RT-PCR扩增蛇床子核糖体蛋白s...核糖体蛋白S13(Ribosomal protein S13,RPS13)属于核糖体蛋白家族,在核糖体中的生物学功能主要是维持核糖体大小亚基之间的动态连接。从蛇床子[Cnidium monnieri(L.)Cuss.]的根、茎、叶组织中提取总RNA,通过RT-PCR扩增蛇床子核糖体蛋白s13(rps13)的开放阅读框(ORF),成功获得了一段453 bp的基因,该基因片段编码151个氨基酸的蛋白;将获得的目的基因rps13 ORF连接到表达载体p ET22b(+)中,并进一步转化到大肠杆菌表达菌中诱导目的基因体外表达;通过对蛋白结构进行分析表明蛇床子RPS13含有6个α螺旋;对蛇床子RPS13进行系统进化分析表明该蛋白在进化上具有高度保守性,与植物类胡萝卜的RPS13亲缘关系最近。蛇床子RPS13蛋白在体外的成功表达以及对其进行系统的生物信息学分析为进一步深入研究该蛋白的结构和功能奠定了理论基础。展开更多
In the work,rGO nanosheet is synthesized using the typical Hummer’s method,then Cu12Sb4 S13 quantum dots@rGO composites are prepared by solvent thermal method,and Cu12Sb4 S13 quantum dots with the average size of 5 n...In the work,rGO nanosheet is synthesized using the typical Hummer’s method,then Cu12Sb4 S13 quantum dots@rGO composites are prepared by solvent thermal method,and Cu12Sb4 S13 quantum dots with the average size of 5 nm are densely distributed on the surface of rGO sheet.NH3 gas response of Cu12Sb4 S13quantum dots@rGO nanosheet composites at room te mperature of 25℃is enhanced compared with the pure Cu12Sb4 S13 quantum dots and rGO nanosheet,and the composites possess an excellent stability during the humidity range of 45%-80%with a low detection limit of 1 ppm,which is related with the intrinsic hydrophobicity characteristic of Cu12Sb4 S13 quantum dots.It also proves that Cu12Sb4 S13quantum dots@rGO nanosheet composites have a quite high selectivity towards ammonia compared with ethanol,methanol,acetone,toluene,hydrogen sulfide and nitrogen dioxide at room temperature.The gas sensing mechanism of the composites is discussed primarily.展开更多
文摘针对深井与超深井高温重载工况下S13Cr油管表面裂纹易引发失效的问题,基于失效评估图开展安全评定研究。通过单轴拉伸与热膨胀试验获取40℃、80℃、120℃、160℃下S13Cr的力学参数与热膨胀系数,得到了S13Cr的本构参数;建立Φ88.9 mm S13Cr油管三维有限元模型,计算环向裂纹的应力强度因子与塑性极限载荷;基于R6规范确定失效评估曲线,通过断裂比(K_(r))与载荷比(L_(r))进行安全评定。结果表明,800 kN轴向拉力与30 MPa压差工况下,裂纹深度a/t=0.3~0.7、形状比a/c=0.8~1.2的S13Cr油管评定点均位于FAD图安全区。
基金supported by the National Natural Science Foundation of China(No.11674258)the 111 Project(No.B18038)+4 种基金Key projects of Natural Science Foundation of Hubei Province(No.2019CFA044)Applied Basic Research Program of Wuhan(No.2018010401011278)Science and Technology Innovation Program of Hubei Province(No.2018B KJ005)Natural Science Foundation of Hunan Province,China(No.2018JJ3527)Students Innovation and Entrepreneurship Training Program(No.20181049721003)。
文摘In the work,rGO nanosheet is synthesized using the typical Hummer’s method,then Cu12Sb4 S13 quantum dots@rGO composites are prepared by solvent thermal method,and Cu12Sb4 S13 quantum dots with the average size of 5 nm are densely distributed on the surface of rGO sheet.NH3 gas response of Cu12Sb4 S13quantum dots@rGO nanosheet composites at room te mperature of 25℃is enhanced compared with the pure Cu12Sb4 S13 quantum dots and rGO nanosheet,and the composites possess an excellent stability during the humidity range of 45%-80%with a low detection limit of 1 ppm,which is related with the intrinsic hydrophobicity characteristic of Cu12Sb4 S13 quantum dots.It also proves that Cu12Sb4 S13quantum dots@rGO nanosheet composites have a quite high selectivity towards ammonia compared with ethanol,methanol,acetone,toluene,hydrogen sulfide and nitrogen dioxide at room temperature.The gas sensing mechanism of the composites is discussed primarily.
