In this paper, the glycoconjugated volatile compounds of four Croatian Satureja species (Satureja montana L., S. cuneifolia Ten., S. subspicata Vis. and endemic S. visianii Silic) were investigated. Content and compos...In this paper, the glycoconjugated volatile compounds of four Croatian Satureja species (Satureja montana L., S. cuneifolia Ten., S. subspicata Vis. and endemic S. visianii Silic) were investigated. Content and composition of these compounds were examined depending on the stage of plant development. GC and GC–MS analysis of volatile aglycones revealed twenty-one compounds. Thymoquinone, geraniol and carvacrol were detected in all vegetative phases of the investigated plants. Other quantitatively important aglycones were eugenol and thymol of S. montana, phenyl ethyl alcohol, benzene acetaldehyde, borneol, α-terpineol, thymol and eugenol of S. cuneifolia, phenyl ethyl alcohol, benzene acet-aldehyde, terpinen-4-ol, α-terpineol and β-ionone of S. subspicata and camphor, thymol and 8a-acetoxylemolol of S. visianii. Moderate similarity in the chemical composition of essential oils and volatile aglycones of investigated plant species indicate that many biologically active compounds are glycosylated and accumulate as non-volatile glycosides.展开更多
目的探讨动脉粥样硬化(AS)患者口腔菌群多样性与炎症因子水平的特征及关联。方法纳入AS患者50例与健康对照50例,采集唾液和血样进行16S rRNA测序、IL-6、IL-1β、TNF-α、CRP检测;比较两组菌群结构与炎症指标差异,并进行Spearman相关和...目的探讨动脉粥样硬化(AS)患者口腔菌群多样性与炎症因子水平的特征及关联。方法纳入AS患者50例与健康对照50例,采集唾液和血样进行16S rRNA测序、IL-6、IL-1β、TNF-α、CRP检测;比较两组菌群结构与炎症指标差异,并进行Spearman相关和多因素回归分析。结果AS组IL-6、IL-1β、TNF-α、CRP水平均显著高于对照组(IL-6:8.24±2.15 vs 6.15±1.76,P<0.01;CRP:7.42±2.41 vs 3.98±1.57,P<0.01);口腔菌群Shannon指数低于对照组(4.38±0.55 vs 4.61±0.52,P=0.040);Fusobacterium与CRP、IL-6正相关(r=0.41、0.36,均P<0.05)。多因素回归提示Fusobacterium丰度及IL-6、CRP水平均为AS潜在危险因素。结论AS患者口腔菌群多样性下降伴随炎症显著增高,二者或通过免疫和代谢途径相互作用,为AS的风险评估与干预策略提供新的思路。展开更多
针对触摸屏监控系统不能满足大中型立体仓库对数据进行存储和处理的功能需求,在Visual Studio 2019集成开发环境中,采用C#语言开发一套立体仓库上位机控制系统。以多线程的方式实时读取库位信息;以S7-1200 PLC作为主控制器,设计产品的...针对触摸屏监控系统不能满足大中型立体仓库对数据进行存储和处理的功能需求,在Visual Studio 2019集成开发环境中,采用C#语言开发一套立体仓库上位机控制系统。以多线程的方式实时读取库位信息;以S7-1200 PLC作为主控制器,设计产品的自动入库和自动出库程序流程,采用SCL语言设计了库位先进先出的控制程序。C#和S7-1200 PLC之间采用S7通信的方式控制立体仓库的出入库操作和库位信息采集,3年的现场运行情况表明,整个系统能在上位机上对立体仓库进行手动控制和自动控制,能精确快速地进行入库出库操作,运行平稳,上位机上能正确实时显示库位信息,达到了预期的结果。展开更多
食源性蛋白淀粉样纤维化聚集具有独特的结构特性,蚕豆11S蛋白(fava bean 11S protein,FP)作为一种可持续蛋白资源,表现出巨大的潜力。该研究探究了蚕豆11S蛋白淀粉样纤维化聚集(fibrotic aggregation of 11S protein in fava bean,FPF)...食源性蛋白淀粉样纤维化聚集具有独特的结构特性,蚕豆11S蛋白(fava bean 11S protein,FP)作为一种可持续蛋白资源,表现出巨大的潜力。该研究探究了蚕豆11S蛋白淀粉样纤维化聚集(fibrotic aggregation of 11S protein in fava bean,FPF)在形成过程中的动态演变,包括其结构表征和功能特性。6 g/100 mL的FP通过酸热处理(pH 2,85℃)不同时间(0~24 h)后得到FPF。处理后的样品通过硫黄素T、荧光、二酪氨酸、透射电子显微镜、傅里叶红外光谱等进行结构表征,结果表明FP先在酸热过程中水解成多肽,再自组装成富含β-折叠结构的FPF(由0 h的34.44%增加到24 h的45.89%)。通过起泡性、乳化性和凝胶特性等对FPF功能特性进行表征,与FP相比,反应24 h后的FPF具有更好的起泡性、乳化性和凝胶特性。此外,FPF在体外细胞实验中没有表现出细胞毒性。研究结果为FPF的形成规律提供了理论支撑。展开更多
文摘In this paper, the glycoconjugated volatile compounds of four Croatian Satureja species (Satureja montana L., S. cuneifolia Ten., S. subspicata Vis. and endemic S. visianii Silic) were investigated. Content and composition of these compounds were examined depending on the stage of plant development. GC and GC–MS analysis of volatile aglycones revealed twenty-one compounds. Thymoquinone, geraniol and carvacrol were detected in all vegetative phases of the investigated plants. Other quantitatively important aglycones were eugenol and thymol of S. montana, phenyl ethyl alcohol, benzene acetaldehyde, borneol, α-terpineol, thymol and eugenol of S. cuneifolia, phenyl ethyl alcohol, benzene acet-aldehyde, terpinen-4-ol, α-terpineol and β-ionone of S. subspicata and camphor, thymol and 8a-acetoxylemolol of S. visianii. Moderate similarity in the chemical composition of essential oils and volatile aglycones of investigated plant species indicate that many biologically active compounds are glycosylated and accumulate as non-volatile glycosides.
文摘目的探讨动脉粥样硬化(AS)患者口腔菌群多样性与炎症因子水平的特征及关联。方法纳入AS患者50例与健康对照50例,采集唾液和血样进行16S rRNA测序、IL-6、IL-1β、TNF-α、CRP检测;比较两组菌群结构与炎症指标差异,并进行Spearman相关和多因素回归分析。结果AS组IL-6、IL-1β、TNF-α、CRP水平均显著高于对照组(IL-6:8.24±2.15 vs 6.15±1.76,P<0.01;CRP:7.42±2.41 vs 3.98±1.57,P<0.01);口腔菌群Shannon指数低于对照组(4.38±0.55 vs 4.61±0.52,P=0.040);Fusobacterium与CRP、IL-6正相关(r=0.41、0.36,均P<0.05)。多因素回归提示Fusobacterium丰度及IL-6、CRP水平均为AS潜在危险因素。结论AS患者口腔菌群多样性下降伴随炎症显著增高,二者或通过免疫和代谢途径相互作用,为AS的风险评估与干预策略提供新的思路。
文摘针对触摸屏监控系统不能满足大中型立体仓库对数据进行存储和处理的功能需求,在Visual Studio 2019集成开发环境中,采用C#语言开发一套立体仓库上位机控制系统。以多线程的方式实时读取库位信息;以S7-1200 PLC作为主控制器,设计产品的自动入库和自动出库程序流程,采用SCL语言设计了库位先进先出的控制程序。C#和S7-1200 PLC之间采用S7通信的方式控制立体仓库的出入库操作和库位信息采集,3年的现场运行情况表明,整个系统能在上位机上对立体仓库进行手动控制和自动控制,能精确快速地进行入库出库操作,运行平稳,上位机上能正确实时显示库位信息,达到了预期的结果。
