In the western segment of the East Kunlun Orogen(WEKO),muscovite granite and garnet granite from the Hureguole intrusion exhibit zircon U-Pb ages of 435-442 Ma.Geochemically,these rocks contain high concentrations of ...In the western segment of the East Kunlun Orogen(WEKO),muscovite granite and garnet granite from the Hureguole intrusion exhibit zircon U-Pb ages of 435-442 Ma.Geochemically,these rocks contain high concentrations of SiO_(2),K_(2)O and Al_(2)O_(3),with low concentrations of TiO_(2)and MgO,indicating a peraluminous high-K calc-alkaline affinity.They are enriched in Rb,Th,U and LREE,depleted in Eu,Ba,Sr and Ti,being classified as S-type granites.Negative whole-rockε_(Nd)(t)values(-9.8 to-9.1)and zirconε_(Hf)(t)values(-11.6 to-8.2)for those granites indicate that they were derived from partial melting of pelitic rocks in the Paleoproterozoic Baishahe Formation of the Jinshuikou Group.Based on the collected zircon ages,Cambrian-Devonian magmatic activity in the WEKO was divided into three stages:early(446-520 Ma),middle(427-441 Ma)and late(372-424 Ma)stages.Statistically,whole-rock Nd and zircon Hf isotope data(ε_(Nd)(t)/T_(DM)^(Nd),ε_(Hf)(t)/T_(DMC)^(Hf))from Paleozoic igneous rocks in the WEKO reveal a magma source that was initially dominated by depleted mantle components in the northward subduction stage of the Proto-Tethy an Ocean plate(446-520 Ma),shifting to predominantly crustal sources during the closure period of the North Qimantagh back-arc basin(427-441 Ma),then to crust-mantle mixed sources in the post-collision stage(372-424 Ma).展开更多
A systematic study of early Paleozoic S-type granites in Pinghe enhances our understanding of the tectonic evolution of proto-Tethys and provides a foundation for exploring rare metal deposits in the region.The Pinghe...A systematic study of early Paleozoic S-type granites in Pinghe enhances our understanding of the tectonic evolution of proto-Tethys and provides a foundation for exploring rare metal deposits in the region.The Pinghe granites consist of monzogranite and leucogranite.Zircon U-Pb dating shows that the emplacement ages of the monzogranite and leucogranite are 502.0 Ma and 500.9 Ma,respectively.All samples have high SiO_(2) content and a weakly to strongly peraluminous character(A/CNK=1.08-1.23),consistent with S-type granites.The monzogranite has relatively high CaO,Sr,Ba,and CaO/Na_(2)O ratios but lower Rb.In contrast,the leucogranite has lower CaO,Sr,Ba,and CaO/Na_(2)O ratios but higher Rb.The similar ε_(Nd)(t)values(−9.3 to−8.4)and Pb isotopic compositions((^(206)Pb/^(204)Pb)t=18.03-19.36,(^(207)Pb/^(204)Pb)t=15.66-15.76,(^(208)Pb/^(204)Pb)t=37.97-38.55)suggest that the monzogranite formed through partial melting of crustal greywacke,while the leucogranite originated from partial melting of crustal pelite.Regional geological studies suggest that these S-type granites in Pinghe were emplaced in an active continental margin setting,associated with the westward subduction of the proto-Tethys oceanic slab.The geochemical characteristics of leucogranite are consistent with those of tungsten-tin-related granites,indicating significant metallogenic potential for W and Sn deposits.展开更多
The acceleration saltation of the traditional S-type acceleration model in the speed planning of the NURBS curve will result in the vibration and flexible impact of the machine tool.It will affect the surface quality ...The acceleration saltation of the traditional S-type acceleration model in the speed planning of the NURBS curve will result in the vibration and flexible impact of the machine tool.It will affect the surface quality of the components.The high speed smooth S-type acceleration and deceleration model deals with flexible impact,but the calculation is tedious.Aimed at the above problems,the traditional S-type acceleration and deceleration model is improved to make the jerk change linearly at a certain slope to reduce the flexible impact.Before the speed planning,it is needed to find the arc length and curvature of each point on the NURBS curve with a tiny step,and to determine the speed sensitivity point on the curve accordingly.According to the speed sensitive point,the NURBS curve is segmented.The attribute parameters of each section are determined by adaptive speed planning.Then,the speed planning can be performed on the NURBS curve according to the speed characteristics classification.The simulation results show that the algorithm can effectively reduce the flexible impact,improve the machining precision and efficiency,and simplify the classification of speed characteristics.展开更多
The Pengshan Sn-polymetallic ore field is located in the southeastern part of the Yangtze block,spanning the southeast edge of the MLYDZ and the northern edge of the mid-segment of the Jiangnan Uplift,and on one side ...The Pengshan Sn-polymetallic ore field is located in the southeastern part of the Yangtze block,spanning the southeast edge of the MLYDZ and the northern edge of the mid-segment of the Jiangnan Uplift,and on one side of the MLYDZ.The studies of LA-ICP-MS zircon U-Pb chronology and petrogeochemistry for Early Cretaceous acid granites from the Pengshan ore field were carried out in this paper.We report zircon U-Pb geochronology and whole-rock geochemistry for acid granites in the Pengshan ore field.The zircon U-Pb ages of the muscovite-granite,biotite adamellite and granite-porphyry are 127.6±1.7 Ma,126.9±1.6 Ma and 126.6±2.0 Ma,respectively.The granites in Pengshan are characterized by a high silicon content and are rich in alkali.They belong to high-potassium,calc-alkaline,peraluminous granite.The rocks have a relatively high Rb/Ba ratio,and the data points for muscovite-granite and biotite adamellite all fall within the clay-rich sources region,near the pelite-derived end-member,showing that the Pengshan muscovite-granite and biotite adamellite mainly originated from the partial melting of metapelites with high maturity.The transformation of the compressional and extensional tectonics in this region approximately 128 Ma obviously lags behind that in the mid-segment of the Jiangnan Uplift(135 Ma),but occurred earlier than the MLYDZ(126 Ma).The Pengshan ore field extends from the mid-segment of the Jiangnan Uplift to the MLYDZ.Although the tectonic stress field is constrained by the combination of the two secondary tectonic units,the time of tectonic system transformation is closer to the MLYDZ because the spatial orientation of the area is enclosed in the MLYDZ.Relevant geophysical and drilling data confirm the rationality of Pengshan-Ao'xia as a multi-center vertical zoning ore field,and show the scientificity of the prospecting idea of abutting joint between the north-west of Pengshan area and the south-east of Ao'xia area.展开更多
Fiber cladding surface plasmon resonance(SPR)sensors have few structures,and a clad SPR sensor based on S-type fiber is proposed in this paper.This new type of fiber cladding SPR sensor was formed by electrofusing an ...Fiber cladding surface plasmon resonance(SPR)sensors have few structures,and a clad SPR sensor based on S-type fiber is proposed in this paper.This new type of fiber cladding SPR sensor was formed by electrofusing an S-shaped structure on the fiber to couple the light in the fiber core to the cladding.In this paper,the effects of fiber parameters on the performance of the sensor were studied by simulation and experiment.Based on the conclusion that the smaller the core diameter is,the closer the working band of the SPR resonance is to long wavelengths,and that the geometric characteristics mean that a multimode fiber can receive the fiber cladding light from a small core diameter few-mode fiber,a dual channel SPR sensor with a double S-type fiber cascade was proposed.In the refractive index detection range of 1.333–1.385refractive index units(RIU),the resonant working band of channel I is 627.66 nm–759.78 nm,with an average sensitivity of 2540.77 nm/RIU,and the resonant working band of channel II is 518.24 nm–658.2 nm,with an average sensitivity of2691.54 nm/RIU.The processing method for the S-type fiber cladding SPR sensor is simple,effectively solving the problem of this type of SPR sensor structure and the difficult realization of a dual channel.The sensor is expected to be used in the fields of medical treatment and biological analysis.展开更多
The central wavelength of the first Bragg scattering bandgap is approximately twice that of the lattice.Therefore,a low-frequency Bragg scattering bandgap with a small structural dimension for phononic crystals is dif...The central wavelength of the first Bragg scattering bandgap is approximately twice that of the lattice.Therefore,a low-frequency Bragg scattering bandgap with a small structural dimension for phononic crystals is difficult to obtain.In this study,a folded S-type periodic structure is developed to reduce the dimension in the direction of vibration suppression by folding unit cells.According to the foregoing,an improved folded S-type periodic structure with different unit cell arrangements is designed to widen the bandgap frequency range.Energy band diagrams and frequency responses are calculated based on the Bloch theory and using the finite element method.Furthermore,a prototype of the improved folded S-type periodic structure is fabricated using a three-dimensional printing technique,and a vibration experiment is conducted.To verify the vibration reduction performance of the structure,numerical simulation and experimental results are compared.This type of folded periodic structure can effectively reduce dimensions to satisfy the dimension requirements pertaining to the direction of vibration suppression.Hence,the foregoing can aid in promoting the use of elastic bandgap structures in engineering.展开更多
1 Introduction Hetai district,which is a mountainous area,situated on Guangning and Zhaoqing city,west Guangdong Province.Hetai district is generally located on southwest of South China Caledonian fold belt,east margi...1 Introduction Hetai district,which is a mountainous area,situated on Guangning and Zhaoqing city,west Guangdong Province.Hetai district is generally located on southwest of South China Caledonian fold belt,east margin of Yunkai post-Caledonian uplift.Multiple type granites are widely distributed in Hetai district,including Caledonian,Indosinian and Yanshanian granites.Based on different展开更多
Objective:To determine the genetic diversity of Plasmodium(P.)knowlesi isolates from Sabah,Malaysian Borneo and Peninsular Malaysia,targeting the S-type SSU rRNA gene and including aspects of natural selection and hap...Objective:To determine the genetic diversity of Plasmodium(P.)knowlesi isolates from Sabah,Malaysian Borneo and Peninsular Malaysia,targeting the S-type SSU rRNA gene and including aspects of natural selection and haplotype.Methods:Thirty-nine blood samples infected with P.knowlesi were collected in Sabah,Malaysian Borneo and Peninsular Malaysia.The S-type SSU rRNA gene was amplified using polymerase chain reaction,cloned into a vector,and sequenced.The natural selection and haplotype of the S-type SSU rRNA gene sequences were determined using DnaSP v6 and illustrated using NETWORK v10.This study's 39 S-type SSU rRNA sequences and eight sequences from the Genbank database were subjected to phylogenetic analysis using MEGA 11.Results:Overall,the phylogenetic analysis showed no evidence of a geographical cluster of P.knowlesi isolates from different areas in Malaysia based on the S-type SSU rRNA gene sequences.The S-type SSU rRNA gene sequences were relatively conserved and with a purifying effect.Haplotype sharing of the S-type SSU rRNA gene was observed between the P.knowlesi isolates in Sabah,Malaysian Borneo,but not between Sabah,Malaysian Borneo and Peninsular Malaysia.Conclusions:This study suggests that the S-type SSU rRNA gene of P.knowlesi isolates in Sabah,Malaysian Borneo,and Peninsular Malaysia has fewer polymorphic sites,representing the conservation of the gene.These features make the S-type SSU rRNA gene suitable for comparative studies,such as determining the evolutionary relationships and common ancestry among P.knowlesi species.展开更多
The effects of Sonic hedgehog(Shh) signaling pathway activation on S-type neuroblastoma(NB) cell lines and its role in NB tumorigenesis were investigated.Immunohistochemistry was used to detect the expression of Shh p...The effects of Sonic hedgehog(Shh) signaling pathway activation on S-type neuroblastoma(NB) cell lines and its role in NB tumorigenesis were investigated.Immunohistochemistry was used to detect the expression of Shh pathway components— Patched1(PTCH1) and Gli1 in 40 human primary NB samples.Western blotting and RT-PCR were used to examine the protein expression and mRNA levels of PTCH1 and Gli1 in three kinds of S-type NB cell lines(SK-N-AS,SK-N-SH and SHEP1),respectively.Exogenous Shh was administrated to activate Shh signaling pathway while cyclopamine was used as a selective antagonist of Shh pathway.S-type NB cell lines were treated with different concentrations of Shh or/and cyclopamine for different durations.Cell viability was measured by using MTT method.Apoptosis rate and cell cycle were assayed by flow cytometry.The xenograft experiments were used to evaluate the role of Shh pathway in tumor growth in immunodeficient mice.High-level expression of PTCH1 and Gli1 was detected in both NB samples and S-type NB cell lines.Cyclopamine decreased the survival rate of the three cell lines while Shh increased it,and the inhibition effects of cyclopamine could be partially reversed by shh pre-treatment.Cyclopamine induced the cell apoptosis and the cell cycle arrest in G0/G1 phase,while Shh induced the reverse effects and could partially prevent effects of cyclopamine.Cyclopamine could also inhibit the growth of NB in vivo.Our studies revealed that activation of the Shh pathway is important for survival and proliferation of S-type NB cells in vivo and in vitro through affecting cell apoptosis and cell cycle,suggesting a new therapeutic approach to NB.展开更多
The western margin of the Yangtze Block hosts diverse Neoproterozoic igneous rocks,with exposed S-type granites serving as key indicators for deciphering regional geological evolution.This study focuses on the Jiudaow...The western margin of the Yangtze Block hosts diverse Neoproterozoic igneous rocks,with exposed S-type granites serving as key indicators for deciphering regional geological evolution.This study focuses on the Jiudaowan granite pluton,located on the western margin of the Yangtze Block,through systematic petrographic,whole-rock geochemical,zircon and monazite U-Pb geochronology,and whole-rock Nd isotopic analyses aiming to elucidate its petrogenesis and tectonic significance.The Jiudaowan granite pluton is a composite body,consisting of the Luotaijiu,Jiudaowan,and Daheishan units,characterized by biotite monzogranites,muscovite-plagioclase granites,and two-mica monzogranites,respectively.LA-ICP-MS zircon and monazite U-Pb dating reveals crystallization ages between 832 and 798 Ma.The three units are peraluminous,containing minerals such as muscovite,garnet,and tourma-line,and exhibiting high SiO_(2)(72.99-77.83 wt%),Al_(2)O_(3)(12.36-15.02 wt%),and A/CNK values(1.06-1.43),con-firming their classification as peraluminous S-type granites.Compositional variations within the Jiudaowan granite pluton are primarily controlled by protolith composition and melting mechanisms.The pluton is distinguished by low CaO/Na_(2)O ratios(0.02-0.18),high Rb/Sr(0.83-113)and Rb/Ba(0.33-15.2)ratios,and negativeεNd(t)values(−13.6 to−9.1),indicating derivation from partial melting of het-erogeneous metasedimentary sources.MgO,TiO_(2),Rb/Sr,and whole-rock Zr saturation temperatures suggest that the Luotaijiu and Daheishan units formed via biotite dehydration melting,whereas the Jiudaowan unit resulted from muscovite dehydration melting.Additionally,the Jiudaowan granite pluton displays a clear negative correlation between Al_(2)O_(3),CaO,Fe_(2)O_(3)T,MgO,TiO_(2),and SiO_(2),along with pronounced Eu negative anomalies and depletions in Sr and Ti,suggesting fractional crystallization of feldspar,mica,and Fe-Ti oxides during magma emplacement.Similarly,variable incompatible element ratios of Nb/U(1.07-18.97)and Nb/La(0.24-26.88)further indicate minor crustal assimilation and contamination during magma evolution.Integrating regional geological data,we propose that the Jiudaowan pluton formed during crustal thickening associated with post-collisional extension,likely related to the breakup of the Rodinia supercontinent.展开更多
基金funded by the China Postdoctoral Science Foundation(Grant No.2019M663959XB)the Geological Survey and Development Bureau of Qinghai Province 2019 Geological Survey Project(2019[45])the Key Laboratory of Deep Dynamics of the Ministry of Natural Resources,Open Research Project 2019。
文摘In the western segment of the East Kunlun Orogen(WEKO),muscovite granite and garnet granite from the Hureguole intrusion exhibit zircon U-Pb ages of 435-442 Ma.Geochemically,these rocks contain high concentrations of SiO_(2),K_(2)O and Al_(2)O_(3),with low concentrations of TiO_(2)and MgO,indicating a peraluminous high-K calc-alkaline affinity.They are enriched in Rb,Th,U and LREE,depleted in Eu,Ba,Sr and Ti,being classified as S-type granites.Negative whole-rockε_(Nd)(t)values(-9.8 to-9.1)and zirconε_(Hf)(t)values(-11.6 to-8.2)for those granites indicate that they were derived from partial melting of pelitic rocks in the Paleoproterozoic Baishahe Formation of the Jinshuikou Group.Based on the collected zircon ages,Cambrian-Devonian magmatic activity in the WEKO was divided into three stages:early(446-520 Ma),middle(427-441 Ma)and late(372-424 Ma)stages.Statistically,whole-rock Nd and zircon Hf isotope data(ε_(Nd)(t)/T_(DM)^(Nd),ε_(Hf)(t)/T_(DMC)^(Hf))from Paleozoic igneous rocks in the WEKO reveal a magma source that was initially dominated by depleted mantle components in the northward subduction stage of the Proto-Tethy an Ocean plate(446-520 Ma),shifting to predominantly crustal sources during the closure period of the North Qimantagh back-arc basin(427-441 Ma),then to crust-mantle mixed sources in the post-collision stage(372-424 Ma).
基金funded by the Science and Technology Department of Yunnan Province(202303AA080006)the National Natural Science Foundation of China(41972312 and 41672329).
文摘A systematic study of early Paleozoic S-type granites in Pinghe enhances our understanding of the tectonic evolution of proto-Tethys and provides a foundation for exploring rare metal deposits in the region.The Pinghe granites consist of monzogranite and leucogranite.Zircon U-Pb dating shows that the emplacement ages of the monzogranite and leucogranite are 502.0 Ma and 500.9 Ma,respectively.All samples have high SiO_(2) content and a weakly to strongly peraluminous character(A/CNK=1.08-1.23),consistent with S-type granites.The monzogranite has relatively high CaO,Sr,Ba,and CaO/Na_(2)O ratios but lower Rb.In contrast,the leucogranite has lower CaO,Sr,Ba,and CaO/Na_(2)O ratios but higher Rb.The similar ε_(Nd)(t)values(−9.3 to−8.4)and Pb isotopic compositions((^(206)Pb/^(204)Pb)t=18.03-19.36,(^(207)Pb/^(204)Pb)t=15.66-15.76,(^(208)Pb/^(204)Pb)t=37.97-38.55)suggest that the monzogranite formed through partial melting of crustal greywacke,while the leucogranite originated from partial melting of crustal pelite.Regional geological studies suggest that these S-type granites in Pinghe were emplaced in an active continental margin setting,associated with the westward subduction of the proto-Tethys oceanic slab.The geochemical characteristics of leucogranite are consistent with those of tungsten-tin-related granites,indicating significant metallogenic potential for W and Sn deposits.
基金the National Key Basic Research Program of China(973 Program)(No.2014CB046501)。
文摘The acceleration saltation of the traditional S-type acceleration model in the speed planning of the NURBS curve will result in the vibration and flexible impact of the machine tool.It will affect the surface quality of the components.The high speed smooth S-type acceleration and deceleration model deals with flexible impact,but the calculation is tedious.Aimed at the above problems,the traditional S-type acceleration and deceleration model is improved to make the jerk change linearly at a certain slope to reduce the flexible impact.Before the speed planning,it is needed to find the arc length and curvature of each point on the NURBS curve with a tiny step,and to determine the speed sensitivity point on the curve accordingly.According to the speed sensitive point,the NURBS curve is segmented.The attribute parameters of each section are determined by adaptive speed planning.Then,the speed planning can be performed on the NURBS curve according to the speed characteristics classification.The simulation results show that the algorithm can effectively reduce the flexible impact,improve the machining precision and efficiency,and simplify the classification of speed characteristics.
基金supported by China Geological Survey Development Research Center Program(Grant No.DD2016005234)Geological exploration fund Program of Jiangxi Province(Grant No.20160010)Jiangxi Bureau of Geology&Mineral Exploration Program(Grant No.201698)。
文摘The Pengshan Sn-polymetallic ore field is located in the southeastern part of the Yangtze block,spanning the southeast edge of the MLYDZ and the northern edge of the mid-segment of the Jiangnan Uplift,and on one side of the MLYDZ.The studies of LA-ICP-MS zircon U-Pb chronology and petrogeochemistry for Early Cretaceous acid granites from the Pengshan ore field were carried out in this paper.We report zircon U-Pb geochronology and whole-rock geochemistry for acid granites in the Pengshan ore field.The zircon U-Pb ages of the muscovite-granite,biotite adamellite and granite-porphyry are 127.6±1.7 Ma,126.9±1.6 Ma and 126.6±2.0 Ma,respectively.The granites in Pengshan are characterized by a high silicon content and are rich in alkali.They belong to high-potassium,calc-alkaline,peraluminous granite.The rocks have a relatively high Rb/Ba ratio,and the data points for muscovite-granite and biotite adamellite all fall within the clay-rich sources region,near the pelite-derived end-member,showing that the Pengshan muscovite-granite and biotite adamellite mainly originated from the partial melting of metapelites with high maturity.The transformation of the compressional and extensional tectonics in this region approximately 128 Ma obviously lags behind that in the mid-segment of the Jiangnan Uplift(135 Ma),but occurred earlier than the MLYDZ(126 Ma).The Pengshan ore field extends from the mid-segment of the Jiangnan Uplift to the MLYDZ.Although the tectonic stress field is constrained by the combination of the two secondary tectonic units,the time of tectonic system transformation is closer to the MLYDZ because the spatial orientation of the area is enclosed in the MLYDZ.Relevant geophysical and drilling data confirm the rationality of Pengshan-Ao'xia as a multi-center vertical zoning ore field,and show the scientificity of the prospecting idea of abutting joint between the north-west of Pengshan area and the south-east of Ao'xia area.
基金the National Natural Science Foundation of China(Grant No.61705025)Chongqing Natural Science Foundation(Grant Nos.cstc2019jcyjmsxmX0431 and cstc2018jcyjAX0817)+2 种基金the Science and Technology Project Affiliated to the Education Department of Chongqing Municipality(Grant Nos.KJQN201801217,KJQN201901226,KJQN202001214,and KJ1710247)Chongqing Key Laboratory of Geological Environment Monitoring and Disaster Early-Warning in Three Gorges Reservoir Area(Grant Nos.ZD2020A0103 and ZD2020A0102)Fundamental Research Funds for Chongqing Three Gorges University of China(Grant No.19ZDPY08).
文摘Fiber cladding surface plasmon resonance(SPR)sensors have few structures,and a clad SPR sensor based on S-type fiber is proposed in this paper.This new type of fiber cladding SPR sensor was formed by electrofusing an S-shaped structure on the fiber to couple the light in the fiber core to the cladding.In this paper,the effects of fiber parameters on the performance of the sensor were studied by simulation and experiment.Based on the conclusion that the smaller the core diameter is,the closer the working band of the SPR resonance is to long wavelengths,and that the geometric characteristics mean that a multimode fiber can receive the fiber cladding light from a small core diameter few-mode fiber,a dual channel SPR sensor with a double S-type fiber cascade was proposed.In the refractive index detection range of 1.333–1.385refractive index units(RIU),the resonant working band of channel I is 627.66 nm–759.78 nm,with an average sensitivity of 2540.77 nm/RIU,and the resonant working band of channel II is 518.24 nm–658.2 nm,with an average sensitivity of2691.54 nm/RIU.The processing method for the S-type fiber cladding SPR sensor is simple,effectively solving the problem of this type of SPR sensor structure and the difficult realization of a dual channel.The sensor is expected to be used in the fields of medical treatment and biological analysis.
基金supported by the National Natural Science Foundation of China(Nos.12072086,12211540384 and 11761131006)the Fundamental Research Funds for the Central Universities(No.3072022CF0203).
文摘The central wavelength of the first Bragg scattering bandgap is approximately twice that of the lattice.Therefore,a low-frequency Bragg scattering bandgap with a small structural dimension for phononic crystals is difficult to obtain.In this study,a folded S-type periodic structure is developed to reduce the dimension in the direction of vibration suppression by folding unit cells.According to the foregoing,an improved folded S-type periodic structure with different unit cell arrangements is designed to widen the bandgap frequency range.Energy band diagrams and frequency responses are calculated based on the Bloch theory and using the finite element method.Furthermore,a prototype of the improved folded S-type periodic structure is fabricated using a three-dimensional printing technique,and a vibration experiment is conducted.To verify the vibration reduction performance of the structure,numerical simulation and experimental results are compared.This type of folded periodic structure can effectively reduce dimensions to satisfy the dimension requirements pertaining to the direction of vibration suppression.Hence,the foregoing can aid in promoting the use of elastic bandgap structures in engineering.
基金co-funded by the China Geological Survey (No.12120114052801)the DREAM project of MOST, China (NO. 2016YFC0600401)
文摘1 Introduction Hetai district,which is a mountainous area,situated on Guangning and Zhaoqing city,west Guangdong Province.Hetai district is generally located on southwest of South China Caledonian fold belt,east margin of Yunkai post-Caledonian uplift.Multiple type granites are widely distributed in Hetai district,including Caledonian,Indosinian and Yanshanian granites.Based on different
基金This study was supported by the Ministry of Higher Education,Malaysia(FRGS0322-SG-1/2013)Universiti Malaysia Sabah(GUG0521-2/2020).
文摘Objective:To determine the genetic diversity of Plasmodium(P.)knowlesi isolates from Sabah,Malaysian Borneo and Peninsular Malaysia,targeting the S-type SSU rRNA gene and including aspects of natural selection and haplotype.Methods:Thirty-nine blood samples infected with P.knowlesi were collected in Sabah,Malaysian Borneo and Peninsular Malaysia.The S-type SSU rRNA gene was amplified using polymerase chain reaction,cloned into a vector,and sequenced.The natural selection and haplotype of the S-type SSU rRNA gene sequences were determined using DnaSP v6 and illustrated using NETWORK v10.This study's 39 S-type SSU rRNA sequences and eight sequences from the Genbank database were subjected to phylogenetic analysis using MEGA 11.Results:Overall,the phylogenetic analysis showed no evidence of a geographical cluster of P.knowlesi isolates from different areas in Malaysia based on the S-type SSU rRNA gene sequences.The S-type SSU rRNA gene sequences were relatively conserved and with a purifying effect.Haplotype sharing of the S-type SSU rRNA gene was observed between the P.knowlesi isolates in Sabah,Malaysian Borneo,but not between Sabah,Malaysian Borneo and Peninsular Malaysia.Conclusions:This study suggests that the S-type SSU rRNA gene of P.knowlesi isolates in Sabah,Malaysian Borneo,and Peninsular Malaysia has fewer polymorphic sites,representing the conservation of the gene.These features make the S-type SSU rRNA gene suitable for comparative studies,such as determining the evolutionary relationships and common ancestry among P.knowlesi species.
基金supported by a grant from the National Natural Sciences Foundation of China (No.30600189)
文摘The effects of Sonic hedgehog(Shh) signaling pathway activation on S-type neuroblastoma(NB) cell lines and its role in NB tumorigenesis were investigated.Immunohistochemistry was used to detect the expression of Shh pathway components— Patched1(PTCH1) and Gli1 in 40 human primary NB samples.Western blotting and RT-PCR were used to examine the protein expression and mRNA levels of PTCH1 and Gli1 in three kinds of S-type NB cell lines(SK-N-AS,SK-N-SH and SHEP1),respectively.Exogenous Shh was administrated to activate Shh signaling pathway while cyclopamine was used as a selective antagonist of Shh pathway.S-type NB cell lines were treated with different concentrations of Shh or/and cyclopamine for different durations.Cell viability was measured by using MTT method.Apoptosis rate and cell cycle were assayed by flow cytometry.The xenograft experiments were used to evaluate the role of Shh pathway in tumor growth in immunodeficient mice.High-level expression of PTCH1 and Gli1 was detected in both NB samples and S-type NB cell lines.Cyclopamine decreased the survival rate of the three cell lines while Shh increased it,and the inhibition effects of cyclopamine could be partially reversed by shh pre-treatment.Cyclopamine induced the cell apoptosis and the cell cycle arrest in G0/G1 phase,while Shh induced the reverse effects and could partially prevent effects of cyclopamine.Cyclopamine could also inhibit the growth of NB in vivo.Our studies revealed that activation of the Shh pathway is important for survival and proliferation of S-type NB cells in vivo and in vitro through affecting cell apoptosis and cell cycle,suggesting a new therapeutic approach to NB.
基金supported by the Deep Earth Probe and Mineral Resources Exploration-National Science and Technology Major Project(Grant No.2024ZD1001600)National Natural Science Foundation of China(Grants Nos.42302223,42162012 and 42402069)Yunnan Fundamental Research Projects(Grants No.202401CF070093).
文摘The western margin of the Yangtze Block hosts diverse Neoproterozoic igneous rocks,with exposed S-type granites serving as key indicators for deciphering regional geological evolution.This study focuses on the Jiudaowan granite pluton,located on the western margin of the Yangtze Block,through systematic petrographic,whole-rock geochemical,zircon and monazite U-Pb geochronology,and whole-rock Nd isotopic analyses aiming to elucidate its petrogenesis and tectonic significance.The Jiudaowan granite pluton is a composite body,consisting of the Luotaijiu,Jiudaowan,and Daheishan units,characterized by biotite monzogranites,muscovite-plagioclase granites,and two-mica monzogranites,respectively.LA-ICP-MS zircon and monazite U-Pb dating reveals crystallization ages between 832 and 798 Ma.The three units are peraluminous,containing minerals such as muscovite,garnet,and tourma-line,and exhibiting high SiO_(2)(72.99-77.83 wt%),Al_(2)O_(3)(12.36-15.02 wt%),and A/CNK values(1.06-1.43),con-firming their classification as peraluminous S-type granites.Compositional variations within the Jiudaowan granite pluton are primarily controlled by protolith composition and melting mechanisms.The pluton is distinguished by low CaO/Na_(2)O ratios(0.02-0.18),high Rb/Sr(0.83-113)and Rb/Ba(0.33-15.2)ratios,and negativeεNd(t)values(−13.6 to−9.1),indicating derivation from partial melting of het-erogeneous metasedimentary sources.MgO,TiO_(2),Rb/Sr,and whole-rock Zr saturation temperatures suggest that the Luotaijiu and Daheishan units formed via biotite dehydration melting,whereas the Jiudaowan unit resulted from muscovite dehydration melting.Additionally,the Jiudaowan granite pluton displays a clear negative correlation between Al_(2)O_(3),CaO,Fe_(2)O_(3)T,MgO,TiO_(2),and SiO_(2),along with pronounced Eu negative anomalies and depletions in Sr and Ti,suggesting fractional crystallization of feldspar,mica,and Fe-Ti oxides during magma emplacement.Similarly,variable incompatible element ratios of Nb/U(1.07-18.97)and Nb/La(0.24-26.88)further indicate minor crustal assimilation and contamination during magma evolution.Integrating regional geological data,we propose that the Jiudaowan pluton formed during crustal thickening associated with post-collisional extension,likely related to the breakup of the Rodinia supercontinent.
