In cultivated rice ( Oryza sativa L.), F-1 pollen sterility is controlled by at least 6 loci of the F, pollen sterility genes. To map S-b, one of loci, rice variety Taichung 65 (T65) carrying S-b(j)/S-b(j) and its nea...In cultivated rice ( Oryza sativa L.), F-1 pollen sterility is controlled by at least 6 loci of the F, pollen sterility genes. To map S-b, one of loci, rice variety Taichung 65 (T65) carrying S-b(j)/S-b(j) and its near-isogenic line TIST2 carrying S-b(i)/S-b(i) were used to develop the mapping population. One hundred and fifty-eight microsatellite markers were selected to survey T65 and TISL2. RM13 on chromosome 5 was found to be polymorphic between them. Cosegregation indicated that RM13 was closely linked with locus S-b. Eleven RFLP markers were selected on the corresponding region from the genetic map of Rice Genome Research Program (RGP) of Japan to convert into sequence-tagged site (STS) markers. Amplicon length polymorphism (ALP) was carried out, but none of them was found to be polymorphic between T65 and TISL2. Then PCR-based RFLP (PBR) was done using six 4-nucleotide recognizing restriction endonucleases. Polymorphism was detected when PCR products of R830STS and R2213SSTS were digested with Taq I. Genetic analysis indicated that the distance between locus S-b and markers, R830STS, RM13 and R2213SSTS were 3.3 cM (centi-Morgan), 5.2 cM and 5.5 cM, respectively. These PCR-based markers could be directly used in marker-assisted selection. The technical system combining genetic mapping and PCR-based marker-assisted selection will facilitate the development of molecular breeding.展开更多
广播式自动相关监视(ADS-B)报头检测方法的优劣直接决定着接收机的性能。为了解决脉冲匹配检测方法受脉冲能量影响较大的不足,提出了一种基带归一化的互相关报头检测方法。首先将基带信号根据动态门限进行归一化,然后将其与标准报头作...广播式自动相关监视(ADS-B)报头检测方法的优劣直接决定着接收机的性能。为了解决脉冲匹配检测方法受脉冲能量影响较大的不足,提出了一种基带归一化的互相关报头检测方法。首先将基带信号根据动态门限进行归一化,然后将其与标准报头作互相关,最后通过相关峰检测来判定信号的存在和到达时间。与主流基于脉冲位置和上升沿的检测方法相比,该方法隐含地利用了脉冲位置、上升沿、下降沿、脉冲宽度、非脉冲区等多个信息用于检测报头,且无需进行下行格式(DF)认证。仿真结果表明:该方法在信噪比大于2 d B时检测性能略优,在信噪比小于2 d B时检测性能与主流检测方法相差1 d B。在实际接收实验中,以该方法为基础的信号接收效果良好,在视距内可以形成稳定连续的航迹,证明了该方法的实用性。展开更多
文摘In cultivated rice ( Oryza sativa L.), F-1 pollen sterility is controlled by at least 6 loci of the F, pollen sterility genes. To map S-b, one of loci, rice variety Taichung 65 (T65) carrying S-b(j)/S-b(j) and its near-isogenic line TIST2 carrying S-b(i)/S-b(i) were used to develop the mapping population. One hundred and fifty-eight microsatellite markers were selected to survey T65 and TISL2. RM13 on chromosome 5 was found to be polymorphic between them. Cosegregation indicated that RM13 was closely linked with locus S-b. Eleven RFLP markers were selected on the corresponding region from the genetic map of Rice Genome Research Program (RGP) of Japan to convert into sequence-tagged site (STS) markers. Amplicon length polymorphism (ALP) was carried out, but none of them was found to be polymorphic between T65 and TISL2. Then PCR-based RFLP (PBR) was done using six 4-nucleotide recognizing restriction endonucleases. Polymorphism was detected when PCR products of R830STS and R2213SSTS were digested with Taq I. Genetic analysis indicated that the distance between locus S-b and markers, R830STS, RM13 and R2213SSTS were 3.3 cM (centi-Morgan), 5.2 cM and 5.5 cM, respectively. These PCR-based markers could be directly used in marker-assisted selection. The technical system combining genetic mapping and PCR-based marker-assisted selection will facilitate the development of molecular breeding.
文摘广播式自动相关监视(ADS-B)报头检测方法的优劣直接决定着接收机的性能。为了解决脉冲匹配检测方法受脉冲能量影响较大的不足,提出了一种基带归一化的互相关报头检测方法。首先将基带信号根据动态门限进行归一化,然后将其与标准报头作互相关,最后通过相关峰检测来判定信号的存在和到达时间。与主流基于脉冲位置和上升沿的检测方法相比,该方法隐含地利用了脉冲位置、上升沿、下降沿、脉冲宽度、非脉冲区等多个信息用于检测报头,且无需进行下行格式(DF)认证。仿真结果表明:该方法在信噪比大于2 d B时检测性能略优,在信噪比小于2 d B时检测性能与主流检测方法相差1 d B。在实际接收实验中,以该方法为基础的信号接收效果良好,在视距内可以形成稳定连续的航迹,证明了该方法的实用性。
