Shoot meristems used for the study were exercised from the in vitro regenerated shoots cultured on MS medium supplemented with 0.5 mg/L of BAP for multiplication. The sensitivity of the in vitro regenerated was studie...Shoot meristems used for the study were exercised from the in vitro regenerated shoots cultured on MS medium supplemented with 0.5 mg/L of BAP for multiplication. The sensitivity of the in vitro regenerated was studied using shoot meristems of 0.5 cm. Shoot meristems were cultured on medium containing 10-100 mg/l kanamycin to determine the concentration that was lethal for multiple shoot induction and root induction. The response of shoot multiplication decreased (66.2%-6.2%) as the concentration of kanamycin increased (10.0-70.0 mg/L) with complete inhibition of shoot proliferation at 100 mg/L kanamycin. The rooting phase was very sensitive to kanamycin compared to shoot multiplication. The percentage of shoots that rooted decreased (53.8%-4.8%) with increase in the concentration of kanamycin (10.0-70.0 mg/l) on IBA and 2,4-D supplemented medium. For transformation studies, the shoot tips that were infected with Agrobacterium strain were placed on selection medium containing MS medium with 0.5 mg/L BAP and 100 mg/L kanamycin and scored for the putative transformed shoots. An average of 62.2% of shoot tips developed shoot buds from the base and the shoots reached a length of 0.5-1.0 cm at the end of 30 days of culture on the selective medium in comparison to control which showed no response. An average of 66.7% of the regenerated plants showed GUS expression on selection medium where 43.2% and 65% of GUS expression was recorded in the leaves and callus. Leaves and callus induced from the controls did not show GUS activity. Stable integration of nptII gene with the genomic DNA from these transformed plants was confirmed through PCR analysis. Our result presents an efficient regeneration system using in vitro derived shoot meristems for Agrobacterium mediated gene transfer.展开更多
目的评价健康调查简表(36-item short form,SF-36)用于测量库欣综合征患者生活质量的信度和效度。方法 2011年1月至2012年4月便利选取上海市瑞金医院内分泌科的73例库欣综合征患者,采用SF-36对研究对象进行调查,信度检验包括内部一致性...目的评价健康调查简表(36-item short form,SF-36)用于测量库欣综合征患者生活质量的信度和效度。方法 2011年1月至2012年4月便利选取上海市瑞金医院内分泌科的73例库欣综合征患者,采用SF-36对研究对象进行调查,信度检验包括内部一致性信度和折半信度,结构效度检验采用因子分析法。结果 SF-36内部一致性信度的Cronbachα系数为0.822,折半信度Spearman-Brown系数为0.771;将8个维度的得分进行因子分析,提取2个公因子,累计方差贡献率为64.587%。结论中文版SF-36用于评价国内库欣综合征患者的生活质量具有较好的信度和效度,接受性好;在目前国内库欣综合征患者的生活质量研究尚处于初级阶段的前提下,可推广应用。展开更多
文摘Shoot meristems used for the study were exercised from the in vitro regenerated shoots cultured on MS medium supplemented with 0.5 mg/L of BAP for multiplication. The sensitivity of the in vitro regenerated was studied using shoot meristems of 0.5 cm. Shoot meristems were cultured on medium containing 10-100 mg/l kanamycin to determine the concentration that was lethal for multiple shoot induction and root induction. The response of shoot multiplication decreased (66.2%-6.2%) as the concentration of kanamycin increased (10.0-70.0 mg/L) with complete inhibition of shoot proliferation at 100 mg/L kanamycin. The rooting phase was very sensitive to kanamycin compared to shoot multiplication. The percentage of shoots that rooted decreased (53.8%-4.8%) with increase in the concentration of kanamycin (10.0-70.0 mg/l) on IBA and 2,4-D supplemented medium. For transformation studies, the shoot tips that were infected with Agrobacterium strain were placed on selection medium containing MS medium with 0.5 mg/L BAP and 100 mg/L kanamycin and scored for the putative transformed shoots. An average of 62.2% of shoot tips developed shoot buds from the base and the shoots reached a length of 0.5-1.0 cm at the end of 30 days of culture on the selective medium in comparison to control which showed no response. An average of 66.7% of the regenerated plants showed GUS expression on selection medium where 43.2% and 65% of GUS expression was recorded in the leaves and callus. Leaves and callus induced from the controls did not show GUS activity. Stable integration of nptII gene with the genomic DNA from these transformed plants was confirmed through PCR analysis. Our result presents an efficient regeneration system using in vitro derived shoot meristems for Agrobacterium mediated gene transfer.
文摘目的评价健康调查简表(36-item short form,SF-36)用于测量库欣综合征患者生活质量的信度和效度。方法 2011年1月至2012年4月便利选取上海市瑞金医院内分泌科的73例库欣综合征患者,采用SF-36对研究对象进行调查,信度检验包括内部一致性信度和折半信度,结构效度检验采用因子分析法。结果 SF-36内部一致性信度的Cronbachα系数为0.822,折半信度Spearman-Brown系数为0.771;将8个维度的得分进行因子分析,提取2个公因子,累计方差贡献率为64.587%。结论中文版SF-36用于评价国内库欣综合征患者的生活质量具有较好的信度和效度,接受性好;在目前国内库欣综合征患者的生活质量研究尚处于初级阶段的前提下,可推广应用。
