RXLR effectors are pathogenic factors secreted from oomycetes to manipulate the immunity of the host.Typical RXLR effectors contain an RXLR-dEER motif at the N-terminus,whereas atypical RXLRs show variations on this m...RXLR effectors are pathogenic factors secreted from oomycetes to manipulate the immunity of the host.Typical RXLR effectors contain an RXLR-dEER motif at the N-terminus,whereas atypical RXLRs show variations on this motif.The oomycete Phytophthora cactorum is known to infect over 200 plant species,resulting in significant agricultural economic losses.Although genome-wide identification and functional analyses of typical RXLRs from P.cactorum have been performed,little is known of atypical PcaRXLRs.Here,we identified RXLRs,both typical and atypical,in P.cactorum and compared them with those of other oomycete pathogens.Fewer RXLRs were identified in P.cactorum compared with other Phytophthora species,possibly due to fewer duplication events of RXLRs.In contrast,the percentage of atypical RXLRs was higher in P.cactorum than in other species,suggesting significant roles in P.cactorum pathogenesis.Analysis of RXLR gene expression showed that most were transcribed,suggesting their functionality.Transient expression of two atypical RXLRs in Nicotiana benthamiana showed that they induced necrosis dependent on host SGT1 and HSP90.Furthermore,two additional atypical RXLRs suppressed the defense response in N.benthamiana and promoted P.cactorum infection.These results demonstrate the vital role of atypical RXLRs in P.cactorum and provide valuable information on their evolutionary patterns and interactions with host plants.展开更多
An accurate assessment of host and pathogen gene expression during infection is critical for understanding the molecular aspects of host-pathogen interactions.Often,pathogen-derived transcripts are difficult to ascert...An accurate assessment of host and pathogen gene expression during infection is critical for understanding the molecular aspects of host-pathogen interactions.Often,pathogen-derived transcripts are difficult to ascertain at early infection stages owing to the unfavourable transcript representation compared to the host genes.In this study,we compare two sequencing techniques,RNAseq and enrichment sequencing(RenSeq and PenSeq)of cDNA,to investigate gene expression patterns in the doubled monoploid potato(DM)infected with the late blight pathogen Phytophthora infestans.Our results reveal distinct advantages of cDNA RenSeq and PenSeq over traditional RNAseq in terms of target gene representation and transcriptional quantification at early infection stages.Throughout the infection time course,cDNA enrichment sequencing enables transcriptomic analyses for more targeted host and pathogen genes.For highly expressed genes that were sampled in parallel by both cDNA enrichment and RNAseq,a high level of concordance in expression profiles is observed,indicative of at least semi-quantitative gene expression representation following enrichment.展开更多
Phytophthora pathogens secrete a large arsenal of effectors that manipulate host processes to create an environment conducive to pathogen colonization. However, the underlying mechanisms by which Phytophthora effector...Phytophthora pathogens secrete a large arsenal of effectors that manipulate host processes to create an environment conducive to pathogen colonization. However, the underlying mechanisms by which Phytophthora effectors manipulate host plant cells still remain largely unclear. In this study, we report that PcAvr3a12, a Phytophthera capsici RXLR effector and a member of the Avr3a effector family, suppresses plant immunity by targeting and inhibiting host plant peptidyl-prolyl cis-trans isomerase （PPlase）. Overexpression of PcAvr3a 12 in Arabidopsis thaliana enhanced plant susceptibility to P. capsici. FKBP15-2, an endoplasmic reticulum （ER）-Iocalized protein, was identified as a host target of PcAvr3a12 during early P. capsici infection. Analyses of A. thaliana T-DNA insertion mutant （fkbp15-2）, RNAi, and overexpression lines consistently showed that FKBP15-2 positively regulates plant immunity in response to Phytophthora infection. FKBP15-2 possesses PPlase activity essential for its contribution to immunity but is directly suppressed by PcAvr3a12. Interestingly, we found that FKBP15-2 is involved in ER stress sensing and is required for ER stress-mediated plant immunity. Taken together, these results suggest that P. capsici deploys an RXLR effector, PcAvr3a12, to facilitate infection by targeting and suppressing a novel ER-Iocalized PPlase, FKBP15-2, which is required for ER stress-mediated plant immunity.展开更多
Plant pathogens rely on effector proteins to suppress host innate immune responses and facilitate colonization.Although the Phytophthora sojae RxLR effector Avh241 promotes Phytophthora infection,the molecular basis o...Plant pathogens rely on effector proteins to suppress host innate immune responses and facilitate colonization.Although the Phytophthora sojae RxLR effector Avh241 promotes Phytophthora infection,the molecular basis of Avh241 virulence remains poorly understood.Here we identified non-race specific disease resistance 1(NDR1)-like proteins,the critical components in plant effector-triggered immunity(ETI)responses,as host targets of Avh241.Avh241 interacts with NDR1 in the plasma membrane and suppresses NDR1-participated ETI responses.Silencing of GmNDR1s increases the susceptibility of soybean to P.sojae infection,and overexpression of GmNDR1s reduces infection,which supports its positive role in plant immunity against P.sojae.Furthermore,we demonstrate that GmNDR1 interacts with itself,and Avh241 probably disrupts the self-association of GmNDR1.These data highlight an effective counter-defense mechanism by which a Phytophthora effector suppresses plant immune responses,likely by disturbing the function of NDR1 during infection.展开更多
RxLR effectors produced by Phytophthora pathogens have been proposed to bind to phosphatidylinositol 3-phosphate （Ptdlns（3）P） to mediate their translocation into host cells and/or to increase their stability in pl...RxLR effectors produced by Phytophthora pathogens have been proposed to bind to phosphatidylinositol 3-phosphate （Ptdlns（3）P） to mediate their translocation into host cells and/or to increase their stability in planta. Since the levels of Ptdlns（3）P in plants are low, we examined whether Phytophthora species may produce Ptdlns（3）P to pro- mote infection. We observed that Ptdlns（3）P-specific GFP biosensors could bind to P. parasitica and P. sojae hyphae dur- ing infection of Nicotiana benthamiana leaves transiently secreting the biosensors, suggesting that the hyphae exposed Ptdlns（3）P on their plasma membrane and/or secreted Ptdlns（3）R Silencing of the phosphatidylinositol 3-kinases （PI3K） genes, treatment with LY294002, or expression of Ptdlns（3）pobinding proteins by P. sojae reduced the virulence of the pathogen on soybean, indicating that pathogen-synthesized Ptdlns（3）P was required for full virulence. Secretion of Ptdlns（3）P-binding proteins or of a PI3P-5-kinase by N. benthamiana leaves significantly increased the level of resist- ance to infection by P. parasitica and P. capsici. Together, our results support the hypothesis that Phytophthora species produce external Ptdlns（3）P to aid in infection, such as to promote entry of RxLR effectors into host cells. Our results derived from P. sojae RxLR effector Avrlb confirm that both the N-terminus and the C-terminus of this effector can bind Ptdlns（3）P.展开更多
Phytophthora pathogens lead to numerous economically damaging plant diseases worldwide,including potato late blight caused by P.infestans and soybean root rot caused by P.sojae.Our previous work showed that Phytophtho...Phytophthora pathogens lead to numerous economically damaging plant diseases worldwide,including potato late blight caused by P.infestans and soybean root rot caused by P.sojae.Our previous work showed that Phytophthora pathogens may generate abundant phosphatidylinositol 3-phosphate(PI3P)to promote infection via direct association with RxLR effectors.Here,we designed a disease control strategy for metabolizing pathogen-derived PI3P by expressing secreted Arabidopsis thaliana phosphatidylinositol-4-phosphate 5-kinase 1(AtPIP5K1),which can phosphorylate PI3P to PI(3,4)P2.We fused AtPIP5K1 with the soybean PR1a signal peptide(SP-PIP5K1)to enable its secretion into the plant apoplast.Transgenic soybean and potato plants expressing SP-PIP5K1 showed substantially enhanced resistance to various P.sojae and P.infestans isolates,respectively.SP-PIP5K1 significantly reduced PI3P accumulation during P.sojae and soybean interaction.Knockout or inhibition of PI3 kinases(PI3Ks)in P.sojae compromised the resistance mediated by SP-PIP5K1,indicating that SP-PIP5K1 action requires a supply of pathogen-derived PI3P.Furthermore,we revealed that SP-PIP5K1 can interfere with the action of P.sojae mediated by the RxLR effector Avr1k.This novel disease control strategy has the potential to confer durable broad-spectrum Phytophthora resistance in plants through a clear mechanism in which catabolism of PI3P interferes with RxLR effector actions.展开更多
基金funded by the Liaoning Applied Basic Research Program(2022JH2/101300284)Liaoning Agricultural Science and Technology Innovation Fund(2022XTCX0503 and 2023QN2417).
文摘RXLR effectors are pathogenic factors secreted from oomycetes to manipulate the immunity of the host.Typical RXLR effectors contain an RXLR-dEER motif at the N-terminus,whereas atypical RXLRs show variations on this motif.The oomycete Phytophthora cactorum is known to infect over 200 plant species,resulting in significant agricultural economic losses.Although genome-wide identification and functional analyses of typical RXLRs from P.cactorum have been performed,little is known of atypical PcaRXLRs.Here,we identified RXLRs,both typical and atypical,in P.cactorum and compared them with those of other oomycete pathogens.Fewer RXLRs were identified in P.cactorum compared with other Phytophthora species,possibly due to fewer duplication events of RXLRs.In contrast,the percentage of atypical RXLRs was higher in P.cactorum than in other species,suggesting significant roles in P.cactorum pathogenesis.Analysis of RXLR gene expression showed that most were transcribed,suggesting their functionality.Transient expression of two atypical RXLRs in Nicotiana benthamiana showed that they induced necrosis dependent on host SGT1 and HSP90.Furthermore,two additional atypical RXLRs suppressed the defense response in N.benthamiana and promoted P.cactorum infection.These results demonstrate the vital role of atypical RXLRs in P.cactorum and provide valuable information on their evolutionary patterns and interactions with host plants.
基金supported by the Rural & Environment Science & Analytical Services (RESAS) Division of the Scottish Government through project JHI-B1-1the Biotechnology and Biological Sciences Research Council (BBSRC) through awards BB/ S015663/1+2 种基金BB/X009068/1Research Leaders 2025 fellowship funded by European Union’s Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreement no. 754380the Research/Scientific Computing teams at The James Hutton Institute and NIAB for providing computational resources and technical support for the “UK’s Crop Diversity Bioinformatics HPC” (BBSRC grant BB/ S019669/1)。
文摘An accurate assessment of host and pathogen gene expression during infection is critical for understanding the molecular aspects of host-pathogen interactions.Often,pathogen-derived transcripts are difficult to ascertain at early infection stages owing to the unfavourable transcript representation compared to the host genes.In this study,we compare two sequencing techniques,RNAseq and enrichment sequencing(RenSeq and PenSeq)of cDNA,to investigate gene expression patterns in the doubled monoploid potato(DM)infected with the late blight pathogen Phytophthora infestans.Our results reveal distinct advantages of cDNA RenSeq and PenSeq over traditional RNAseq in terms of target gene representation and transcriptional quantification at early infection stages.Throughout the infection time course,cDNA enrichment sequencing enables transcriptomic analyses for more targeted host and pathogen genes.For highly expressed genes that were sampled in parallel by both cDNA enrichment and RNAseq,a high level of concordance in expression profiles is observed,indicative of at least semi-quantitative gene expression representation following enrichment.
基金This work was supported by the China Agriculture Research System （CARS-09）, the National Natural Science Foundation of China （31125022 and 31561143007）, and the Program of Introducing Talents of Innovative Discipline to Universities （Project 111） from the State Administration of Foreign Experts Affairs （#B18042）.
文摘Phytophthora pathogens secrete a large arsenal of effectors that manipulate host processes to create an environment conducive to pathogen colonization. However, the underlying mechanisms by which Phytophthora effectors manipulate host plant cells still remain largely unclear. In this study, we report that PcAvr3a12, a Phytophthera capsici RXLR effector and a member of the Avr3a effector family, suppresses plant immunity by targeting and inhibiting host plant peptidyl-prolyl cis-trans isomerase （PPlase）. Overexpression of PcAvr3a 12 in Arabidopsis thaliana enhanced plant susceptibility to P. capsici. FKBP15-2, an endoplasmic reticulum （ER）-Iocalized protein, was identified as a host target of PcAvr3a12 during early P. capsici infection. Analyses of A. thaliana T-DNA insertion mutant （fkbp15-2）, RNAi, and overexpression lines consistently showed that FKBP15-2 positively regulates plant immunity in response to Phytophthora infection. FKBP15-2 possesses PPlase activity essential for its contribution to immunity but is directly suppressed by PcAvr3a12. Interestingly, we found that FKBP15-2 is involved in ER stress sensing and is required for ER stress-mediated plant immunity. Taken together, these results suggest that P. capsici deploys an RXLR effector, PcAvr3a12, to facilitate infection by targeting and suppressing a novel ER-Iocalized PPlase, FKBP15-2, which is required for ER stress-mediated plant immunity.
文摘剑麻是硬质纤维的主要来源,由烟草疫霉(Phytophthora nicotianae)引起的斑马纹病是剑麻生产的重要病害。RXLR效应蛋白是卵菌第一大类效应蛋白,能通过直接的蛋白质相互作用,靶向寄主防卫相关蛋白,干扰和抑制寄主防卫。利用转录组分析不同侵染阶段的烟草疫霉,发现PnRXLR5863表达量较高,并且其编码的蛋白含有信号肽、RXLR-EER和WY结构域,瞬时表达发现PnRXLR5863能引起烟草细胞死亡,推测PnRXLR5863在烟草疫霉侵染剑麻过程发挥重要作用,但是它作用于剑麻的靶蛋白和致病机制还未知。为了筛选PnRXLR5863的剑麻靶蛋白,应用Switching Mechanism at 5'end of RNA Transcript(SMART)和Duplex-Specific Nuclease(DSN)技术构建了剑麻均一化酵母cDNA表达文库,并筛选了与PnRXLR5863相互作用的蛋白。结果显示,构建的文库库容为5.32×10^(7)CFU/mL,文库总克隆数为1.064×10^(8)CFU,文库片段平均大小为1000 bp,重组率为100%;构建的pGBKT7-PnRXLR5863诱饵载体无自激活性;利用酵母双杂交系统,以pGBKT7-PnRXLR5863诱饵载体,从构建的文库中筛选获得23个与PnRXLR5863相互作用的蛋白。获得的候选靶蛋白主要参与分子功能和生物过程,涉及泛素化过程、蛋白合成、蛋白翻译和蛋白修饰等生理事件,暗示PnRXLR5863效应蛋白可能作用于剑麻的蛋白代谢。剑麻均一化酵母表达文库的构建和PnRXLR5863靶蛋白的筛选,为进一步深入研究PnRXLR5863的致病机理奠定基础。
基金supported by the Natural ScienceFoundation of Jiangsu Province(BK20190520)the NationalNatural Science Foundation of China(31721004,32001882)the National Postdoctoral Program for Innovative Talents(BX20180142)。
文摘Plant pathogens rely on effector proteins to suppress host innate immune responses and facilitate colonization.Although the Phytophthora sojae RxLR effector Avh241 promotes Phytophthora infection,the molecular basis of Avh241 virulence remains poorly understood.Here we identified non-race specific disease resistance 1(NDR1)-like proteins,the critical components in plant effector-triggered immunity(ETI)responses,as host targets of Avh241.Avh241 interacts with NDR1 in the plasma membrane and suppresses NDR1-participated ETI responses.Silencing of GmNDR1s increases the susceptibility of soybean to P.sojae infection,and overexpression of GmNDR1s reduces infection,which supports its positive role in plant immunity against P.sojae.Furthermore,we demonstrate that GmNDR1 interacts with itself,and Avh241 probably disrupts the self-association of GmNDR1.These data highlight an effective counter-defense mechanism by which a Phytophthora effector suppresses plant immune responses,likely by disturbing the function of NDR1 during infection.
基金This work was supported by grants from the National Natural Science Foundation of China （No. 30971889）, National Science and Technology Major Projects （No. 2009ZX08005-003B）, and the Natural Science Foundation of Jiangsu Province （No. BK2012027）.We appreciate Prof. Brett Tyler （Oregon State） for manuscript preparation, and Yuanming Zhang （Nanjing Agricultural University） for suggestions in data analysis. No conflict of interest declared.
文摘RxLR effectors produced by Phytophthora pathogens have been proposed to bind to phosphatidylinositol 3-phosphate （Ptdlns（3）P） to mediate their translocation into host cells and/or to increase their stability in planta. Since the levels of Ptdlns（3）P in plants are low, we examined whether Phytophthora species may produce Ptdlns（3）P to pro- mote infection. We observed that Ptdlns（3）P-specific GFP biosensors could bind to P. parasitica and P. sojae hyphae dur- ing infection of Nicotiana benthamiana leaves transiently secreting the biosensors, suggesting that the hyphae exposed Ptdlns（3）P on their plasma membrane and/or secreted Ptdlns（3）R Silencing of the phosphatidylinositol 3-kinases （PI3K） genes, treatment with LY294002, or expression of Ptdlns（3）pobinding proteins by P. sojae reduced the virulence of the pathogen on soybean, indicating that pathogen-synthesized Ptdlns（3）P was required for full virulence. Secretion of Ptdlns（3）P-binding proteins or of a PI3P-5-kinase by N. benthamiana leaves significantly increased the level of resist- ance to infection by P. parasitica and P. capsici. Together, our results support the hypothesis that Phytophthora species produce external Ptdlns（3）P to aid in infection, such as to promote entry of RxLR effectors into host cells. Our results derived from P. sojae RxLR effector Avrlb confirm that both the N-terminus and the C-terminus of this effector can bind Ptdlns（3）P.
基金supported by the National Natural Science Foundation of China,China(32072507,32272495,and 31721004)the Natural Science Foundation of Jiangsu Province,China(BK20220147).
文摘Phytophthora pathogens lead to numerous economically damaging plant diseases worldwide,including potato late blight caused by P.infestans and soybean root rot caused by P.sojae.Our previous work showed that Phytophthora pathogens may generate abundant phosphatidylinositol 3-phosphate(PI3P)to promote infection via direct association with RxLR effectors.Here,we designed a disease control strategy for metabolizing pathogen-derived PI3P by expressing secreted Arabidopsis thaliana phosphatidylinositol-4-phosphate 5-kinase 1(AtPIP5K1),which can phosphorylate PI3P to PI(3,4)P2.We fused AtPIP5K1 with the soybean PR1a signal peptide(SP-PIP5K1)to enable its secretion into the plant apoplast.Transgenic soybean and potato plants expressing SP-PIP5K1 showed substantially enhanced resistance to various P.sojae and P.infestans isolates,respectively.SP-PIP5K1 significantly reduced PI3P accumulation during P.sojae and soybean interaction.Knockout or inhibition of PI3 kinases(PI3Ks)in P.sojae compromised the resistance mediated by SP-PIP5K1,indicating that SP-PIP5K1 action requires a supply of pathogen-derived PI3P.Furthermore,we revealed that SP-PIP5K1 can interfere with the action of P.sojae mediated by the RxLR effector Avr1k.This novel disease control strategy has the potential to confer durable broad-spectrum Phytophthora resistance in plants through a clear mechanism in which catabolism of PI3P interferes with RxLR effector actions.
