BACKGROUND RPF2 is a crucial factor in ribosome synthesis,which has been linked to the development of several cancers.However,the mechanism of RPF2 in gastric carcinogenesis is unclear.AIM To explore the role and mech...BACKGROUND RPF2 is a crucial factor in ribosome synthesis,which has been linked to the development of several cancers.However,the mechanism of RPF2 in gastric carcinogenesis is unclear.AIM To explore the role and mechanism of RPF2 in the pathogenesis of Helicobacter pylori(H.pylori)infection.METHODS GES-1 was co-cultured with H.pylori in vitro to detect changes in the expression of RPF2.Overexpression and silencing of RPF2 were performed.Quantitative realtime polymerase chain reaction(q-PCR)and Western blot(WB)were used to determine mRNA and protein expression of RPF2,protein kinase B(AKT)/mammalian target of rapamycin(mTOR),and epithelial-mesenchymal transitionrelated factors MMP2 and MMP9;cell counting kit 8 and wound healing assays were utilized to evaluate cell viability and migratory capacity;q-PCR,WB,and immunohistochemistry were employed to establish RPF2 expression in cancer tissues.RESULTS H.pylori facilitated RPF2 expression and triggered AKT/mTOR signaling pathway.Functional experiments showed that RPF2 overexpression could promote a series of malignant transformations such as cell proliferation,cell migration and invasion,and further enhance AKT/mTOR signaling pathway activation.RPF2 knockdown had the opposite effect.In addition,RPF2 expression was higher in gastric cancer tissues than in adjacent tissues.CONCLUSION RPF2 plays a significant role in the pathogenic mechanism of H.pylori infection and may be useful in the detection and management of gastric cancer caused by H.pylori infection.展开更多
99%以上的微生物因处于活的但非可培养(viable but non-culturable,VBNC)状态而无法分离培养。复苏促进因子(resuscitation-promoting factors,Rpfs)是培养获取VBNC菌的最重要突破。结合课题组近十余年从环境功能视角利用Rpf复苏培养VBN...99%以上的微生物因处于活的但非可培养(viable but non-culturable,VBNC)状态而无法分离培养。复苏促进因子(resuscitation-promoting factors,Rpfs)是培养获取VBNC菌的最重要突破。结合课题组近十余年从环境功能视角利用Rpf复苏培养VBNC菌的研究,本文在阐述微生物VBNC状态的形成及复苏进展的基础上,从VBNC菌形成及复苏过程出发,探究"探索因子"与群体感应的内在关系。并总结了课题组利用Rpf所复苏培养的具有潜在环境功能的VBNC菌种。本论文将为揭示微生物VBNC状态的形成及复苏机制提供新的思路,并为认识和重新评价Rpf法复苏培养VBNC菌在污染环境微生物修复中的作用提供理论依据。展开更多
为了进一步认识闪电和固、液态降水的关系,本文利用三维雷暴云动力-电耦合数值模式,通过设置敏感性试验组,模拟了一次雷暴过程,分析雷暴中闪电和降水的特征,以及闪电和固、液态降水对垂直风速的依赖关系,探讨闪电与固、液态降水的时空...为了进一步认识闪电和固、液态降水的关系,本文利用三维雷暴云动力-电耦合数值模式,通过设置敏感性试验组,模拟了一次雷暴过程,分析雷暴中闪电和降水的特征,以及闪电和固、液态降水对垂直风速的依赖关系,探讨闪电与固、液态降水的时空分布关系和单次闪电表征的降水量(RPF:rainyields per flash)。结果表明:对流云降水中,液态降水占主要部分,但固态降水比液态降水对于垂直风速的依赖性更强。随着对流的增强,固态降水在总降水中占的比重越来越大。首次放电时间不断提前,闪电峰值落后垂直风速峰值,总闪数一开始随对流的增强而增加,对流一旦增强到一定程度,总闪数则逐渐减小。固态降水和液态降水的开始时间和峰值时间均随着对流的增强而不断提前,而液态降水出现时间和峰值时间均提前于固态降水。雷暴云首次放电的时间滞后于液态降水,而闪电峰值提前固态降水峰值或与固态降水峰值同时产生。雷暴云中的放电活动集中在强降水区域前缘的较弱降水区,强降水区对应的闪电较少,对流的增强会使降水区域面积、降水量和降水强度增加。由于液态降水总量远大于固态降水总量,固、液态RPF的数值相差达到一个量级,但单位时间内固态降水和液态降水增加的速率相近。在单位时间内闪电次数越多,RPF则越小,而固态RPF和闪电次数的线性相关性明显好于液态RPF,所以利用固态降水可以更好地预报闪电。这些结果有助于进一步认识闪电和降水的关系,并可为闪电预报提供新的思路。展开更多
基金Supported by the National Natural Science Foundation of China,No.82160634.
文摘BACKGROUND RPF2 is a crucial factor in ribosome synthesis,which has been linked to the development of several cancers.However,the mechanism of RPF2 in gastric carcinogenesis is unclear.AIM To explore the role and mechanism of RPF2 in the pathogenesis of Helicobacter pylori(H.pylori)infection.METHODS GES-1 was co-cultured with H.pylori in vitro to detect changes in the expression of RPF2.Overexpression and silencing of RPF2 were performed.Quantitative realtime polymerase chain reaction(q-PCR)and Western blot(WB)were used to determine mRNA and protein expression of RPF2,protein kinase B(AKT)/mammalian target of rapamycin(mTOR),and epithelial-mesenchymal transitionrelated factors MMP2 and MMP9;cell counting kit 8 and wound healing assays were utilized to evaluate cell viability and migratory capacity;q-PCR,WB,and immunohistochemistry were employed to establish RPF2 expression in cancer tissues.RESULTS H.pylori facilitated RPF2 expression and triggered AKT/mTOR signaling pathway.Functional experiments showed that RPF2 overexpression could promote a series of malignant transformations such as cell proliferation,cell migration and invasion,and further enhance AKT/mTOR signaling pathway activation.RPF2 knockdown had the opposite effect.In addition,RPF2 expression was higher in gastric cancer tissues than in adjacent tissues.CONCLUSION RPF2 plays a significant role in the pathogenic mechanism of H.pylori infection and may be useful in the detection and management of gastric cancer caused by H.pylori infection.
文摘99%以上的微生物因处于活的但非可培养(viable but non-culturable,VBNC)状态而无法分离培养。复苏促进因子(resuscitation-promoting factors,Rpfs)是培养获取VBNC菌的最重要突破。结合课题组近十余年从环境功能视角利用Rpf复苏培养VBNC菌的研究,本文在阐述微生物VBNC状态的形成及复苏进展的基础上,从VBNC菌形成及复苏过程出发,探究"探索因子"与群体感应的内在关系。并总结了课题组利用Rpf所复苏培养的具有潜在环境功能的VBNC菌种。本论文将为揭示微生物VBNC状态的形成及复苏机制提供新的思路,并为认识和重新评价Rpf法复苏培养VBNC菌在污染环境微生物修复中的作用提供理论依据。
文摘为了进一步认识闪电和固、液态降水的关系,本文利用三维雷暴云动力-电耦合数值模式,通过设置敏感性试验组,模拟了一次雷暴过程,分析雷暴中闪电和降水的特征,以及闪电和固、液态降水对垂直风速的依赖关系,探讨闪电与固、液态降水的时空分布关系和单次闪电表征的降水量(RPF:rainyields per flash)。结果表明:对流云降水中,液态降水占主要部分,但固态降水比液态降水对于垂直风速的依赖性更强。随着对流的增强,固态降水在总降水中占的比重越来越大。首次放电时间不断提前,闪电峰值落后垂直风速峰值,总闪数一开始随对流的增强而增加,对流一旦增强到一定程度,总闪数则逐渐减小。固态降水和液态降水的开始时间和峰值时间均随着对流的增强而不断提前,而液态降水出现时间和峰值时间均提前于固态降水。雷暴云首次放电的时间滞后于液态降水,而闪电峰值提前固态降水峰值或与固态降水峰值同时产生。雷暴云中的放电活动集中在强降水区域前缘的较弱降水区,强降水区对应的闪电较少,对流的增强会使降水区域面积、降水量和降水强度增加。由于液态降水总量远大于固态降水总量,固、液态RPF的数值相差达到一个量级,但单位时间内固态降水和液态降水增加的速率相近。在单位时间内闪电次数越多,RPF则越小,而固态RPF和闪电次数的线性相关性明显好于液态RPF,所以利用固态降水可以更好地预报闪电。这些结果有助于进一步认识闪电和降水的关系,并可为闪电预报提供新的思路。
