Every day,people follow a routine.What is your morning routine?Maybe you wake up at six or seven.You wash your face and brush your hair.You dress for school or work.Maybe you eat breakfast at home.
Objective:To compare the nursing effects of routine care and specialized nursing intervention on patients with dysphagia during acute stroke.Methods:A total of 188 patients with acute stroke and dysphagia who received...Objective:To compare the nursing effects of routine care and specialized nursing intervention on patients with dysphagia during acute stroke.Methods:A total of 188 patients with acute stroke and dysphagia who received treatment at our hospital from May 2022 to December 2024 were selected as study subjects.The patients were randomly divided into a control group and an observation group,with 94 patients in each group.The control group received routine nursing intervention,while the observation group received specialized nursing intervention.The general information,swallowing function,quality of life,and nursing satisfaction were compared between the two groups.Results:There was no statistically significant difference in general information between the two groups(P>0.05).The SAS score of the observation group after nursing was significantly lower than that of the control group(t=19.463,P=0.000<0.001).After intervention,the number of patients with lung infection in the observation group was significantly lower than that in the control group(χ2=7.309,P=0.007<0.01).The overall quality of life score of the observation group was significantly better than that of the control group after intervention(P<0.001).The nursing satisfaction of the observation group was significantly higher than that of the control group(χ^(2)=15.865,P=0.000<0.001).Conclusion:Compared with routine care,specialized nursing intervention can effectively improve the swallowing function of patients with dysphagia during acute stroke,enhance their quality of life,and increase nursing satisfaction.展开更多
Objective:To explore the accuracy and stability of the results of peripheral blood routine tests at different time points after anticoagulation and standing,providing a scientific basis for actual clinical work.Method...Objective:To explore the accuracy and stability of the results of peripheral blood routine tests at different time points after anticoagulation and standing,providing a scientific basis for actual clinical work.Methods:In this study,30 patients who visited the hospital in October 2023 were randomly selected and divided into two groups(15 cases in each group).The same collection method was used for routine blood tests.The tests were performed after anticoagulation and standing for 5 minutes,1 hour,and 5 minutes,2 hours respectively,and the routine blood test indicators at different time points were compared.Results:After comparison,there were no significant differences in the results of routine blood tests at 5 minutes after mixing,anticoagulating,and standing peripheral blood and those at 1 hour and 2 hours(P>0.05).Conclusion:The results of peripheral blood after mixing,anticoagulating,and standing for 5 minutes are stable compared with those after standing for 1 hour and 2 hours.In actual work,the pre-test turnaround time can be appropriately extended.展开更多
Background:In recent years,there has been a growing trend in the utilization of observational studies that make use of routinely collected healthcare data(RCD).These studies rely on algorithms to identify specific hea...Background:In recent years,there has been a growing trend in the utilization of observational studies that make use of routinely collected healthcare data(RCD).These studies rely on algorithms to identify specific health conditions(e.g.,diabetes or sepsis)for statistical analyses.However,there has been substantial variation in the algorithm development and validation,leading to frequently suboptimal performance and posing a significant threat to the validity of study findings.Unfortunately,these issues are often overlooked.Methods:We systematically developed guidance for the development,validation,and evaluation of algorithms designed to identify health status(DEVELOP-RCD).Our initial efforts involved conducting both a narrative review and a systematic review of published studies on the concepts and methodological issues related to algorithm development,validation,and evaluation.Subsequently,we conducted an empirical study on an algorithm for identifying sepsis.Based on these findings,we formulated specific workflow and recommendations for algorithm development,validation,and evaluation within the guidance.Finally,the guidance underwent independent review by a panel of 20 external experts who then convened a consensus meeting to finalize it.Results:A standardized workflow for algorithm development,validation,and evaluation was established.Guided by specific health status considerations,the workflow comprises four integrated steps:assessing an existing algorithm’s suitability for the target health status;developing a new algorithm using recommended methods;validating the algorithm using prescribed performance measures;and evaluating the impact of the algorithm on study results.Additionally,13 good practice recommendations were formulated with detailed explanations.Furthermore,a practical study on sepsis identification was included to demonstrate the application of this guidance.Conclusions:The establishment of guidance is intended to aid researchers and clinicians in the appropriate and accurate development and application of algorithms for identifying health status from RCD.This guidance has the potential to enhance the credibility of findings from observational studies involving RCD.展开更多
本研究以日本黄瓜绿斑驳花叶病毒(kyuri green mottle mosaic virus,KGMMV)为材料,设计1对常规RT-PCR检测引物KGCPN-F/KGCPN-R和1组引物/探针KGM-F/KGM-R/KGM-P,分析测试了2对已发表KGMMV常规RT-PCR检测引物KGCP-F/KGCP-R和KGMP-F/KGMP...本研究以日本黄瓜绿斑驳花叶病毒(kyuri green mottle mosaic virus,KGMMV)为材料,设计1对常规RT-PCR检测引物KGCPN-F/KGCPN-R和1组引物/探针KGM-F/KGM-R/KGM-P,分析测试了2对已发表KGMMV常规RT-PCR检测引物KGCP-F/KGCP-R和KGMP-F/KGMP-R,建立了KGMMV的常规和实时荧光RT-PCR检测方法。结果表明,引物KGCP-F/KGCP-R扩增KGMMV时出现预期大小的条带,扩增黄瓜绿斑驳花叶病毒(cucumber green mottle mosaic virus,CGMMV)时出现非常微弱的条带;引物KGCPN-F/KGCPN-R扩增KGMMV时出现预期大小的条带,扩增小西葫芦绿斑驳花叶病毒(zucchini green mottle mosaic virus,ZGMMV)时出现比预期稍大的条带,通过对PCR产物进行序列测定和分析比对可准确鉴定KGMMV。KGCP-F/KGCP-R和KGCPN-F/KGCPN-R的相对灵敏度分别为10-6和10-5稀释度,适用于KGMMV的常规RT-PCR检测。基于引物探针KGM-F/KGM-R/KGM-P建立的KGMMV实时荧光RT-PCR检测方法能特异性检出KGMMV,相对灵敏度达10-7稀释度,分别比2对常规RT-PCR检测引物高10倍和100倍,适用于瓜类种子中KGMMV的快速检测。展开更多
By modifying the salicylic-acid moiety with electron-withdrawing or –donating groups, three new terbium complexes（LⅠ·Tb, LⅡ·Tb, L·（Ⅲ）Tb） based on tripodal carboxylate ligands were synthesized. D...By modifying the salicylic-acid moiety with electron-withdrawing or –donating groups, three new terbium complexes（LⅠ·Tb, LⅡ·Tb, L·（Ⅲ）Tb） based on tripodal carboxylate ligands were synthesized. Due to different pull-push electronic effects of ligands, the fluorescence intensities of these terbium complexes significantly varied, that is： LⅡ·Tb 〉L（Ⅲ）·Tb〉 LⅠ·Tb. Meanwhile, the characteristic peaks at 492 nm（5D4→7F6） and 547 nm（5 D4→7F5） showed ＂Off–On–Off＂ fluorescence response to various p H conditions,which indicated that all of them can be used as the highly sensitive pH fluorescent probes. Notably, using LⅡ·Tb with the best fluorescence performance as a probe, some patients＇ urine samples can be easily monitored through the response triggered by pH value. Therefore, LⅡ·Tb has the potential to auxiliarily diagnose some diseases in clinical practice through p H detection of routine urine test.展开更多
BACKGROUND Rivaroxaban is a non-vitamin K antagonist oral anticoagulant that does not require coagulation monitoring based on current recommendations. Our goal is to explore whether routine coagulation monitoring shou...BACKGROUND Rivaroxaban is a non-vitamin K antagonist oral anticoagulant that does not require coagulation monitoring based on current recommendations. Our goal is to explore whether routine coagulation monitoring should not be required for all patients receiving oral rivaroxaban, what relationship between routine coagulation abnormalities and bleeding, and how to deal with the above clinical situations through our case and review of the literature.CASE SUMMARY We report a 67-year-old woman with a history of atrial fibrillation who presented to the hospital with worsening dyspnea and cough. Based on electrocardiogram,venous compression ultrasonography, and computed tomography pulmonary angiography, the diagnosis of atrial fibrillation, deep venous thrombosis, and acute pulmonary embolism was confirmed. Her coagulation assays and renal function were normal on admission; she was not underweight, did not have a history of hemorrhagic disease, and her CHA2 DS2-VAS, HAS-BLED, and simplified Pulmonary Embolism Severity Index scores were 3, 0, and 0,respectively. Oral rivaroxaban(15 mg twice daily) was administered. The following day, she presented gastrointestinal and gum bleeding, combined with coagulation abnormalities. Following cessation of rivaroxaban, her bleeding stopped and tests improved over the next 2 d. Rivaroxaban was begun again 3 d after recovery. However, she again presented with gastrointestinal and gum bleeding and the abnormal tests, and the therapy was discontinued. At 30-d follow-up after discharge, she presented normal coagulation tests without bleeding.CONCLUSION Although current guidelines recommend that using non-vitamin K antagonist oral anticoagulants including rivaroxaban do not require coagulation monitoring,a small number of patients may develop routine coagulation test changes and bleeding during rivaroxaban therapy, especially in the elderly. Clinicians should pay attention to these patients and further obtain evidence in practice.展开更多
[Objectives] In this study,routine blood indexes of Guike pig were detected to clarify quantity variation and morphology distribution of different functional cells（ red blood cells,white blood cells and platelets）,t...[Objectives] In this study,routine blood indexes of Guike pig were detected to clarify quantity variation and morphology distribution of different functional cells（ red blood cells,white blood cells and platelets）,thus providing reference data for immunological evaluation and disease resistance research of Guike pig. [Methods] Blood samples were collected from forty-four 49-day-old healthy Guike pigs（ 22 males and 22 females） once a week for seven consecutive weeks to detect routine blood indexes,and the results were compared with routine blood indexes of Landrace pig and Enshi black pig. [Results]Among red blood cell indexes of Guike pig at different day ages,red blood cell count（ RBC） and hemoglobin count（ HGB） both reached the lowest levels（ 6. 85 × 10^12/L and 97. 1 g/L）at 70 days of age,which were significantly lower than the corresponding indexes at 49,56,63,84 and 91 days of age（ P 〈 0. 05,similarly hereinafter）; mean corpuscular hemoglobin（ MCH） and mean corpuscular hemoglobin concentration（ MCHC） demonstrated basically the same variation pattern,which both reached the lowest levels（ 15. 11 pg and 275. 70 g/L） at 70 days of age and reached the highest levels（ 16. 40 pg and 314. 30 g/L） at 91 days of age; hematocrit（ HCT）,mean corpuscular volume（ MCV） and red blood cell distribution width（ RDW） exhibited relatively stable changes with no significant differences among different day ages（ P 〉 0. 05,similarly hereinafter）. Among white blood cell indexes of Guike pig at different day ages,white blood cell count（ WBC） reached the lowest level（ 13. 71 × 10^9/L） at 63 days of age and reached the highest level（ 17. 52 × 10^9/L） at 84 days of age,with significant differences; neutrophil count（ NEUT）reached the lowest level（ 3. 61 × 10^9/L） at 70 days of age,which was significantly lower than that at 49,56 and 77 days of age; lymphocyte count（ LYMPH） exhibited relatively stable changes and was not significantly affected by the increase in day age. Among platelet indexes of Guike pig at different day ages,platelet count（ PLT）,thrombocytocrit（ PCT） and platelet volume distribution width（ PDW） exhibited relatively stable changes among different day ages,which were not significantly affected by the increase in day age. Compared to 35-day-old Landrace pig and 5-month-old Enshi black pig,most routine blood indexes of Guike pig were similar to or ranged between those of Landrace pig and Enshi black pig except HCT,mean platelet volume（ MPV）,PCT and PDW. [Conclusions]Guike pig has a certain uniqueness in routine blood indexes,which has developed a stable immune system at 49-91 days of age.展开更多
文摘Every day,people follow a routine.What is your morning routine?Maybe you wake up at six or seven.You wash your face and brush your hair.You dress for school or work.Maybe you eat breakfast at home.
文摘Objective:To compare the nursing effects of routine care and specialized nursing intervention on patients with dysphagia during acute stroke.Methods:A total of 188 patients with acute stroke and dysphagia who received treatment at our hospital from May 2022 to December 2024 were selected as study subjects.The patients were randomly divided into a control group and an observation group,with 94 patients in each group.The control group received routine nursing intervention,while the observation group received specialized nursing intervention.The general information,swallowing function,quality of life,and nursing satisfaction were compared between the two groups.Results:There was no statistically significant difference in general information between the two groups(P>0.05).The SAS score of the observation group after nursing was significantly lower than that of the control group(t=19.463,P=0.000<0.001).After intervention,the number of patients with lung infection in the observation group was significantly lower than that in the control group(χ2=7.309,P=0.007<0.01).The overall quality of life score of the observation group was significantly better than that of the control group after intervention(P<0.001).The nursing satisfaction of the observation group was significantly higher than that of the control group(χ^(2)=15.865,P=0.000<0.001).Conclusion:Compared with routine care,specialized nursing intervention can effectively improve the swallowing function of patients with dysphagia during acute stroke,enhance their quality of life,and increase nursing satisfaction.
基金Project of Guangdong Provincial Medical Science and Technology Research Fund(A2022011)Major Science and Technology Project of Shenzhen Nanshan District Health System(NSZD2023067)Sub-project of Education(Health)Science and Technology Project of Nanshan District Technology Research and Development and Creative Design Project in Shenzhen(NS2022002)。
文摘Objective:To explore the accuracy and stability of the results of peripheral blood routine tests at different time points after anticoagulation and standing,providing a scientific basis for actual clinical work.Methods:In this study,30 patients who visited the hospital in October 2023 were randomly selected and divided into two groups(15 cases in each group).The same collection method was used for routine blood tests.The tests were performed after anticoagulation and standing for 5 minutes,1 hour,and 5 minutes,2 hours respectively,and the routine blood test indicators at different time points were compared.Results:After comparison,there were no significant differences in the results of routine blood tests at 5 minutes after mixing,anticoagulating,and standing peripheral blood and those at 1 hour and 2 hours(P>0.05).Conclusion:The results of peripheral blood after mixing,anticoagulating,and standing for 5 minutes are stable compared with those after standing for 1 hour and 2 hours.In actual work,the pre-test turnaround time can be appropriately extended.
基金supported by the National Natural Science Foundation of China(82225049,72104155)the Sichuan Provincial Central Government Guides Local Science and Technology Development Special Project(2022ZYD0127)the 1·3·5 Project for Disciplines of Excellence,West China Hospital,Sichuan University(ZYGD23004).
文摘Background:In recent years,there has been a growing trend in the utilization of observational studies that make use of routinely collected healthcare data(RCD).These studies rely on algorithms to identify specific health conditions(e.g.,diabetes or sepsis)for statistical analyses.However,there has been substantial variation in the algorithm development and validation,leading to frequently suboptimal performance and posing a significant threat to the validity of study findings.Unfortunately,these issues are often overlooked.Methods:We systematically developed guidance for the development,validation,and evaluation of algorithms designed to identify health status(DEVELOP-RCD).Our initial efforts involved conducting both a narrative review and a systematic review of published studies on the concepts and methodological issues related to algorithm development,validation,and evaluation.Subsequently,we conducted an empirical study on an algorithm for identifying sepsis.Based on these findings,we formulated specific workflow and recommendations for algorithm development,validation,and evaluation within the guidance.Finally,the guidance underwent independent review by a panel of 20 external experts who then convened a consensus meeting to finalize it.Results:A standardized workflow for algorithm development,validation,and evaluation was established.Guided by specific health status considerations,the workflow comprises four integrated steps:assessing an existing algorithm’s suitability for the target health status;developing a new algorithm using recommended methods;validating the algorithm using prescribed performance measures;and evaluating the impact of the algorithm on study results.Additionally,13 good practice recommendations were formulated with detailed explanations.Furthermore,a practical study on sepsis identification was included to demonstrate the application of this guidance.Conclusions:The establishment of guidance is intended to aid researchers and clinicians in the appropriate and accurate development and application of algorithms for identifying health status from RCD.This guidance has the potential to enhance the credibility of findings from observational studies involving RCD.
文摘本研究以日本黄瓜绿斑驳花叶病毒(kyuri green mottle mosaic virus,KGMMV)为材料,设计1对常规RT-PCR检测引物KGCPN-F/KGCPN-R和1组引物/探针KGM-F/KGM-R/KGM-P,分析测试了2对已发表KGMMV常规RT-PCR检测引物KGCP-F/KGCP-R和KGMP-F/KGMP-R,建立了KGMMV的常规和实时荧光RT-PCR检测方法。结果表明,引物KGCP-F/KGCP-R扩增KGMMV时出现预期大小的条带,扩增黄瓜绿斑驳花叶病毒(cucumber green mottle mosaic virus,CGMMV)时出现非常微弱的条带;引物KGCPN-F/KGCPN-R扩增KGMMV时出现预期大小的条带,扩增小西葫芦绿斑驳花叶病毒(zucchini green mottle mosaic virus,ZGMMV)时出现比预期稍大的条带,通过对PCR产物进行序列测定和分析比对可准确鉴定KGMMV。KGCP-F/KGCP-R和KGCPN-F/KGCPN-R的相对灵敏度分别为10-6和10-5稀释度,适用于KGMMV的常规RT-PCR检测。基于引物探针KGM-F/KGM-R/KGM-P建立的KGMMV实时荧光RT-PCR检测方法能特异性检出KGMMV,相对灵敏度达10-7稀释度,分别比2对常规RT-PCR检测引物高10倍和100倍,适用于瓜类种子中KGMMV的快速检测。
基金supported by the National Natural Science Foundation of China (Nos. 21572091 and 21472075)the Fundamental Research Funds for the Central Universities (No. lzujbky-2016-51) by Ministry of Education of China
文摘By modifying the salicylic-acid moiety with electron-withdrawing or –donating groups, three new terbium complexes（LⅠ·Tb, LⅡ·Tb, L·（Ⅲ）Tb） based on tripodal carboxylate ligands were synthesized. Due to different pull-push electronic effects of ligands, the fluorescence intensities of these terbium complexes significantly varied, that is： LⅡ·Tb 〉L（Ⅲ）·Tb〉 LⅠ·Tb. Meanwhile, the characteristic peaks at 492 nm（5D4→7F6） and 547 nm（5 D4→7F5） showed ＂Off–On–Off＂ fluorescence response to various p H conditions,which indicated that all of them can be used as the highly sensitive pH fluorescent probes. Notably, using LⅡ·Tb with the best fluorescence performance as a probe, some patients＇ urine samples can be easily monitored through the response triggered by pH value. Therefore, LⅡ·Tb has the potential to auxiliarily diagnose some diseases in clinical practice through p H detection of routine urine test.
文摘BACKGROUND Rivaroxaban is a non-vitamin K antagonist oral anticoagulant that does not require coagulation monitoring based on current recommendations. Our goal is to explore whether routine coagulation monitoring should not be required for all patients receiving oral rivaroxaban, what relationship between routine coagulation abnormalities and bleeding, and how to deal with the above clinical situations through our case and review of the literature.CASE SUMMARY We report a 67-year-old woman with a history of atrial fibrillation who presented to the hospital with worsening dyspnea and cough. Based on electrocardiogram,venous compression ultrasonography, and computed tomography pulmonary angiography, the diagnosis of atrial fibrillation, deep venous thrombosis, and acute pulmonary embolism was confirmed. Her coagulation assays and renal function were normal on admission; she was not underweight, did not have a history of hemorrhagic disease, and her CHA2 DS2-VAS, HAS-BLED, and simplified Pulmonary Embolism Severity Index scores were 3, 0, and 0,respectively. Oral rivaroxaban(15 mg twice daily) was administered. The following day, she presented gastrointestinal and gum bleeding, combined with coagulation abnormalities. Following cessation of rivaroxaban, her bleeding stopped and tests improved over the next 2 d. Rivaroxaban was begun again 3 d after recovery. However, she again presented with gastrointestinal and gum bleeding and the abnormal tests, and the therapy was discontinued. At 30-d follow-up after discharge, she presented normal coagulation tests without bleeding.CONCLUSION Although current guidelines recommend that using non-vitamin K antagonist oral anticoagulants including rivaroxaban do not require coagulation monitoring,a small number of patients may develop routine coagulation test changes and bleeding during rivaroxaban therapy, especially in the elderly. Clinicians should pay attention to these patients and further obtain evidence in practice.
基金Supported by Major Scientific and Technological Special Project of Guangxi Zhuang Autonomous Region"Innovation and Demonstration of Ecological Comprehensive Prevention and Control Technology for Major Diseases of Livestock and Poultry"(GKAA17204057)Natural Science Foundation of Guangxi Zhuang Autonomous Region(2015GXNSFAA139070)+1 种基金Open-end Fund for Key Laboratory of Livestock Genetic Improvement of Guangxi Zhuang Autonomous Region(2014GXKLLGI-07)Project of Aquatic Animal Husbandry Science and Technology Extension and Application of Guangxi Zhuang Autonomous Region(GYMK201528030,GYMK201633034)
文摘[Objectives] In this study,routine blood indexes of Guike pig were detected to clarify quantity variation and morphology distribution of different functional cells（ red blood cells,white blood cells and platelets）,thus providing reference data for immunological evaluation and disease resistance research of Guike pig. [Methods] Blood samples were collected from forty-four 49-day-old healthy Guike pigs（ 22 males and 22 females） once a week for seven consecutive weeks to detect routine blood indexes,and the results were compared with routine blood indexes of Landrace pig and Enshi black pig. [Results]Among red blood cell indexes of Guike pig at different day ages,red blood cell count（ RBC） and hemoglobin count（ HGB） both reached the lowest levels（ 6. 85 × 10^12/L and 97. 1 g/L）at 70 days of age,which were significantly lower than the corresponding indexes at 49,56,63,84 and 91 days of age（ P 〈 0. 05,similarly hereinafter）; mean corpuscular hemoglobin（ MCH） and mean corpuscular hemoglobin concentration（ MCHC） demonstrated basically the same variation pattern,which both reached the lowest levels（ 15. 11 pg and 275. 70 g/L） at 70 days of age and reached the highest levels（ 16. 40 pg and 314. 30 g/L） at 91 days of age; hematocrit（ HCT）,mean corpuscular volume（ MCV） and red blood cell distribution width（ RDW） exhibited relatively stable changes with no significant differences among different day ages（ P 〉 0. 05,similarly hereinafter）. Among white blood cell indexes of Guike pig at different day ages,white blood cell count（ WBC） reached the lowest level（ 13. 71 × 10^9/L） at 63 days of age and reached the highest level（ 17. 52 × 10^9/L） at 84 days of age,with significant differences; neutrophil count（ NEUT）reached the lowest level（ 3. 61 × 10^9/L） at 70 days of age,which was significantly lower than that at 49,56 and 77 days of age; lymphocyte count（ LYMPH） exhibited relatively stable changes and was not significantly affected by the increase in day age. Among platelet indexes of Guike pig at different day ages,platelet count（ PLT）,thrombocytocrit（ PCT） and platelet volume distribution width（ PDW） exhibited relatively stable changes among different day ages,which were not significantly affected by the increase in day age. Compared to 35-day-old Landrace pig and 5-month-old Enshi black pig,most routine blood indexes of Guike pig were similar to or ranged between those of Landrace pig and Enshi black pig except HCT,mean platelet volume（ MPV）,PCT and PDW. [Conclusions]Guike pig has a certain uniqueness in routine blood indexes,which has developed a stable immune system at 49-91 days of age.
