[Objective] By using the genomic DNA of Cymbidium faberi Rolfe as template,the factors that affect the result of ISSR-PCR reaction system were researched and the optimal system was established.[Method] The genomic DNA...[Objective] By using the genomic DNA of Cymbidium faberi Rolfe as template,the factors that affect the result of ISSR-PCR reaction system were researched and the optimal system was established.[Method] The genomic DNA was extracted from C.faberi Rolfe with method of modified CTAB.Different factors which affected ISSR amplification reaction were optimized.[Result] High-quality genomic DNA was obtained from C.faberi Rolfe.And the optimal reaction system was as follows：25 μl amplification reactions system contained 2.5 μl 10 × PCR buffer,2.0 mmol/L MgCl2,60 ng template DNA,160 μmol/L dNTPs,1.25 U Taq DNA polymerase,0.4 μmol/L ISSR primer and 15.85 μl ddH2O.The optimal amplification procedures were pre-denaturing for 5 min at 94 ℃,followed by 40 cycles of denaturing for 30 s at 94 ℃,annealing for 30 s at a temperature of 2-3 ℃ lower than melting temperature of each primer pair,extension for 50 s at 72 ℃.Then extension step of 7 min at 72 ℃ was performed.[Conclusion] The optimal system could provide a favorable basis for further study on genetic diversity of C.faberi Rolfe by using ISSR molecular marker technique.展开更多
[Objectives] This study was conducted to establish a characteristic fingerprinting research method for the chemical constituents of the Zhuang medicine Pholidota cantonensis Rolfe.and its characteristic fingerprint of...[Objectives] This study was conducted to establish a characteristic fingerprinting research method for the chemical constituents of the Zhuang medicine Pholidota cantonensis Rolfe.and its characteristic fingerprint of chemical constituents,in order to identify P.cantonensis.[Methods] Ten batches of P.cantonensis from different habitats in Guangxi and out of Guangxi (Shaoguan of Guangdong Province) were determined.The materials were extracted in 80% methanol ultrasonically for 1.5 h under the material-to-liquid ratio of 2.5 g/80 ml.The HPLC determination was carried out using an Agilent 1260 HPLP instrument equipped with an Agilent5 TC-C18 (2) column at the column temperature of 30 ℃,the wavelength of 280 nm and the injection volume of 10 μl using acetonitrile-0.4% phosphoric acid as the mobile phase and chlorogenic acid as the reference peak,and the detection took 58 min.[Results] The characteristic fingerprint of the chemical constituents of P.cantonensis were obtained,and the three chromatographic peaks of gallic acid,chlorogenic acid and syringaldehyde were compared.[Conclusions] This study can provide reference for the identification of the Zhuang medicine P.cantonensis and provide guarantee for the clinical use of Chinese medicine.展开更多
[Objectives]Pharmacognosy identification was performed on Xiaohuangcao ( Dendrobium loddigesi Rolfe).[Methods]The medicinal materials were identified through original plants,characters,microscopic structure and thin-l...[Objectives]Pharmacognosy identification was performed on Xiaohuangcao ( Dendrobium loddigesi Rolfe).[Methods]The medicinal materials were identified through original plants,characters,microscopic structure and thin-layer identification characteristics.[Results]D.loddigesi has obvious plant morphology,characters,microscopic structure and thin-layer identification characteristics.The stem of Xiaohuangcao is slender and cylindrical,and the surface is golden yellow;and the fiber bundles outside the vascular bundles are crescent-shaped or semi-circular in the stem transection.For the powder,crystal fiber can be observed;the vascular bundles are embedded with siliceous block cells;and there are more starch grains.In the thin layer chromatography,petroleum ether-ethyl acetate-butanone-glacial acetic acid (8.5∶ 3.5∶ 1.5∶ 5 d) was used as a developing solvent,and 10% sulfuric acid ethanol solution was used as a color developing agent.[Conclusions]The research results provide reference for the application of the medicinal material and the formulation of its related quality standards.展开更多
Research progress of pharmacognostical identification and chemical composition of Pholidota cantonensis Rolfe. was reviewed. This paper will facilitate assurance of the safety of clinical treatment.
Viktor & Rolf由Viktor Horsting和Rolf Snoeren这两位毕业于著名的Arnhem Academy of the Arts的荷兰设计师于1993年在巴黎创建。这个二人组合一出道便以新奇的创意、浓烈的实验色彩和骨子里的Couture情结让人们领略到主流时尚以...Viktor & Rolf由Viktor Horsting和Rolf Snoeren这两位毕业于著名的Arnhem Academy of the Arts的荷兰设计师于1993年在巴黎创建。这个二人组合一出道便以新奇的创意、浓烈的实验色彩和骨子里的Couture情结让人们领略到主流时尚以外的另类精彩。他们的设计一直游走在艺术和时尚之间,以反传统的剪裁、颠覆性的比例、夸张的细节和前卫概念性的秀场布置而闻名,其带点玩味的设计及独特的时装美学概念一直是时装传媒的焦点。展开更多
目的通过研究杀青方式和时间、干燥方式对药用植物独蒜兰Pleione bulbocodioides假鳞茎外观、显微结构和化学成分含量的影响,探索其最佳初加工工艺。方法采用烘箱直接烘干(oven direct drying,OD)、煮制10、20、30 min后烘干(boiling fo...目的通过研究杀青方式和时间、干燥方式对药用植物独蒜兰Pleione bulbocodioides假鳞茎外观、显微结构和化学成分含量的影响,探索其最佳初加工工艺。方法采用烘箱直接烘干(oven direct drying,OD)、煮制10、20、30 min后烘干(boiling for 10,20,30 min and then drying,B10D、B20D、B30D)、隔水蒸制10、20、30 min后烘干(steaming for 10,20,30 min and then drying,S10D、S20D、S30D)和冷冻干燥(freeze drying,FD)共8种方式处理独蒜兰假鳞茎,测定其2-O-葡萄糖基白及苷(dactylorhin A)、白及苷和山药素Ⅲ的含量。通过构建HPLC指纹图谱,并进行相似度分析(similarity analysis,SA)、层次聚类分析(hierarchical cluster analysis,HCA)和主成分分析(principal component analysis,PCA)评价加工方法对假鳞茎质量的影响。通过外观特征、组织结构及粉末显微结构观察,评估加工方法对假鳞茎结构的影响。以dactylorhin A、白及苷、山药素Ⅲ、醇溶性浸出物、水溶性浸出物、折干率、水分、灰分和酸不溶性灰分含量为指标,利用熵权法、层次分析法(analytic hierarchy process,AHP)和逼近理想解排序法(technique for order preference by similarity to ideal solution,TOPSIS)综合评价不同加工方法对假鳞茎品质的影响。结果8种加工方法所得样品的HPLC指纹图谱相似度为0.681~0.994。其中,FD样品的相似度最低,且HCA和PCA均将其单独归为一类,其余7种加工方法的样品则聚为另一类。通过HPLC指纹图谱24个共有峰鉴定出3个共有峰,分别为dactylorhin A(峰7)、白及苷(峰9)和山药素Ⅲ(峰15)。TOPSIS综合评价结果发现,S10D处理样品的贴近度以及dactylorhin A、白及苷和山药素Ⅲ总含量最高。结论隔水蒸制10 min后烘干是独蒜兰假鳞茎初加工的最优方法,该方法有利于活性成分保留,且工艺简便。为独蒜兰假鳞茎的规范化加工以及山慈菇药材质量标准的完善提供了重要依据。展开更多
Abstract: Two novel triterpenes (1 and 2) were isolated from the orchid Pholidota yunnanensis Rolfe. Using chemical and spectral analyses (UV, IR, MS,1D-NMR and 2D-NMR), these two triterpenes were established as 25-me...Abstract: Two novel triterpenes (1 and 2) were isolated from the orchid Pholidota yunnanensis Rolfe. Using chemical and spectral analyses (UV, IR, MS,1D-NMR and 2D-NMR), these two triterpenes were established as 25-methylenecyclopholidonyl p-hydroxy-trans-cinnamate (1; named pholidotine A) and 25-methylenecyclopholidonyl p-hydroxy-cis-cinnamate (2; named pholidotine B).展开更多
基金Supported by a Program from the State Environmental Protection Administration(20061A0013)~~
文摘[Objective] By using the genomic DNA of Cymbidium faberi Rolfe as template,the factors that affect the result of ISSR-PCR reaction system were researched and the optimal system was established.[Method] The genomic DNA was extracted from C.faberi Rolfe with method of modified CTAB.Different factors which affected ISSR amplification reaction were optimized.[Result] High-quality genomic DNA was obtained from C.faberi Rolfe.And the optimal reaction system was as follows：25 μl amplification reactions system contained 2.5 μl 10 × PCR buffer,2.0 mmol/L MgCl2,60 ng template DNA,160 μmol/L dNTPs,1.25 U Taq DNA polymerase,0.4 μmol/L ISSR primer and 15.85 μl ddH2O.The optimal amplification procedures were pre-denaturing for 5 min at 94 ℃,followed by 40 cycles of denaturing for 30 s at 94 ℃,annealing for 30 s at a temperature of 2-3 ℃ lower than melting temperature of each primer pair,extension for 50 s at 72 ℃.Then extension step of 7 min at 72 ℃ was performed.[Conclusion] The optimal system could provide a favorable basis for further study on genetic diversity of C.faberi Rolfe by using ISSR molecular marker technique.
基金Supported by Collaborative Innovation Center for Zhuang and Yao Medicines(GJKY[2013]20)Guangxi Key Laboratory of Zhuang and Yao Medicine(GKJZ[2014]32)+3 种基金Guangxi Key Discipline of Zhuang Medicine(GJKY[2013]16)Masters Innovation Project of Postgraduate Education Innovation Program of Guangxi University of Chinese Medicine(YJSP201738)Guangxi Key Discipline of Traditional Chinese pharmacology(0501802815)Development of Zhuangyao Huotanmu Capsule for the Treatment of Hepatitis B(NKF[2018]11)
文摘[Objectives] This study was conducted to establish a characteristic fingerprinting research method for the chemical constituents of the Zhuang medicine Pholidota cantonensis Rolfe.and its characteristic fingerprint of chemical constituents,in order to identify P.cantonensis.[Methods] Ten batches of P.cantonensis from different habitats in Guangxi and out of Guangxi (Shaoguan of Guangdong Province) were determined.The materials were extracted in 80% methanol ultrasonically for 1.5 h under the material-to-liquid ratio of 2.5 g/80 ml.The HPLC determination was carried out using an Agilent 1260 HPLP instrument equipped with an Agilent5 TC-C18 (2) column at the column temperature of 30 ℃,the wavelength of 280 nm and the injection volume of 10 μl using acetonitrile-0.4% phosphoric acid as the mobile phase and chlorogenic acid as the reference peak,and the detection took 58 min.[Results] The characteristic fingerprint of the chemical constituents of P.cantonensis were obtained,and the three chromatographic peaks of gallic acid,chlorogenic acid and syringaldehyde were compared.[Conclusions] This study can provide reference for the identification of the Zhuang medicine P.cantonensis and provide guarantee for the clinical use of Chinese medicine.
基金Supported by Guangxi"2011 Collaborative Innovation Center"-Zhuang Yao Medicine Collaborative Innovation Center Project(GJKY[2013]20)Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32)+7 种基金Guangxi Key Discipline:Zhuang Pharmacy(GJKY[2013]16)Bagui Scholars ProjectNational Natural Science Foundation of China(81460587)Nanning Science and Technology Bureau Project(20183046-1)Project of Guangxi Association for Science and TechnologyThe Fourth National General Survey of Chinese Medicine Resources(Guangxi)Pilot Survey Project(GXZYZYPC-2)Guangxi Marine Drug Resources Survey-Qinzhou City(GXZYZYPC13-7-2)Guangxi First-class Discipline:Chinese Pharmacy(0501802803)
文摘[Objectives]Pharmacognosy identification was performed on Xiaohuangcao ( Dendrobium loddigesi Rolfe).[Methods]The medicinal materials were identified through original plants,characters,microscopic structure and thin-layer identification characteristics.[Results]D.loddigesi has obvious plant morphology,characters,microscopic structure and thin-layer identification characteristics.The stem of Xiaohuangcao is slender and cylindrical,and the surface is golden yellow;and the fiber bundles outside the vascular bundles are crescent-shaped or semi-circular in the stem transection.For the powder,crystal fiber can be observed;the vascular bundles are embedded with siliceous block cells;and there are more starch grains.In the thin layer chromatography,petroleum ether-ethyl acetate-butanone-glacial acetic acid (8.5∶ 3.5∶ 1.5∶ 5 d) was used as a developing solvent,and 10% sulfuric acid ethanol solution was used as a color developing agent.[Conclusions]The research results provide reference for the application of the medicinal material and the formulation of its related quality standards.
基金Supported by Zhuang Yao Medicine Collaborative Innovation Center(GJKY[2013]20)Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32)+1 种基金Guangxi Key Discipline:Zhuang pharmacy(GJKY[2013]16)Chinese Traditional Medicine Innovation Theory and Drug Efficacy Study of Bagui Scholars(J13162)
文摘Research progress of pharmacognostical identification and chemical composition of Pholidota cantonensis Rolfe. was reviewed. This paper will facilitate assurance of the safety of clinical treatment.
文摘Viktor & Rolf由Viktor Horsting和Rolf Snoeren这两位毕业于著名的Arnhem Academy of the Arts的荷兰设计师于1993年在巴黎创建。这个二人组合一出道便以新奇的创意、浓烈的实验色彩和骨子里的Couture情结让人们领略到主流时尚以外的另类精彩。他们的设计一直游走在艺术和时尚之间,以反传统的剪裁、颠覆性的比例、夸张的细节和前卫概念性的秀场布置而闻名,其带点玩味的设计及独特的时装美学概念一直是时装传媒的焦点。
文摘目的通过研究杀青方式和时间、干燥方式对药用植物独蒜兰Pleione bulbocodioides假鳞茎外观、显微结构和化学成分含量的影响,探索其最佳初加工工艺。方法采用烘箱直接烘干(oven direct drying,OD)、煮制10、20、30 min后烘干(boiling for 10,20,30 min and then drying,B10D、B20D、B30D)、隔水蒸制10、20、30 min后烘干(steaming for 10,20,30 min and then drying,S10D、S20D、S30D)和冷冻干燥(freeze drying,FD)共8种方式处理独蒜兰假鳞茎,测定其2-O-葡萄糖基白及苷(dactylorhin A)、白及苷和山药素Ⅲ的含量。通过构建HPLC指纹图谱,并进行相似度分析(similarity analysis,SA)、层次聚类分析(hierarchical cluster analysis,HCA)和主成分分析(principal component analysis,PCA)评价加工方法对假鳞茎质量的影响。通过外观特征、组织结构及粉末显微结构观察,评估加工方法对假鳞茎结构的影响。以dactylorhin A、白及苷、山药素Ⅲ、醇溶性浸出物、水溶性浸出物、折干率、水分、灰分和酸不溶性灰分含量为指标,利用熵权法、层次分析法(analytic hierarchy process,AHP)和逼近理想解排序法(technique for order preference by similarity to ideal solution,TOPSIS)综合评价不同加工方法对假鳞茎品质的影响。结果8种加工方法所得样品的HPLC指纹图谱相似度为0.681~0.994。其中,FD样品的相似度最低,且HCA和PCA均将其单独归为一类,其余7种加工方法的样品则聚为另一类。通过HPLC指纹图谱24个共有峰鉴定出3个共有峰,分别为dactylorhin A(峰7)、白及苷(峰9)和山药素Ⅲ(峰15)。TOPSIS综合评价结果发现,S10D处理样品的贴近度以及dactylorhin A、白及苷和山药素Ⅲ总含量最高。结论隔水蒸制10 min后烘干是独蒜兰假鳞茎初加工的最优方法,该方法有利于活性成分保留,且工艺简便。为独蒜兰假鳞茎的规范化加工以及山慈菇药材质量标准的完善提供了重要依据。
文摘Abstract: Two novel triterpenes (1 and 2) were isolated from the orchid Pholidota yunnanensis Rolfe. Using chemical and spectral analyses (UV, IR, MS,1D-NMR and 2D-NMR), these two triterpenes were established as 25-methylenecyclopholidonyl p-hydroxy-trans-cinnamate (1; named pholidotine A) and 25-methylenecyclopholidonyl p-hydroxy-cis-cinnamate (2; named pholidotine B).
