The gel-forming ability of rohu (Labeo rohita) mince with and without egg white powder (EW) was investigated. Gel from washed mince (washed gel) was prepared under two setting conditions: kamaboko (40°C) and modo...The gel-forming ability of rohu (Labeo rohita) mince with and without egg white powder (EW) was investigated. Gel from washed mince (washed gel) was prepared under two setting conditions: kamaboko (40°C) and modori (60°C). The gel-forming ability of kamaboko and modori gels was improved by the addition of EW at 2%. The autolytic inhibition of kamaboko gel was obtained in gel added with 2% EW, and 1% EW of modori gel. No marked change was observed in the TCA-soluble peptide content of either gel with the addition of EW above 1%. No effect on the whiteness of both gels was shown after the addition of EW. The addition of EW exhibited smaller cavities and a more compact fibrous network in microstructure.展开更多
Information regarding molecular characteristics of leptin protein in different animal species in-cluding fish is scarce. With the aim of characterizing the native leptin protein of Indian major carprohu (Labeo rohita)...Information regarding molecular characteristics of leptin protein in different animal species in-cluding fish is scarce. With the aim of characterizing the native leptin protein of Indian major carprohu (Labeo rohita), at molecular level, the present study was designed to isolate rohu leptin from its hepatocytes (the prime source of leptin in fish) and immunobiochemical characterization of the same, subsequently. In the present study, chemical treatment and ultra-sonication technique was used for isolating leptin from rohu liver tissue. Purification of the protein was attempted using affinity column chromatography. The molecular, biophysical and serological characterization of rohu leptin was carried out by 2D-gel electrophoreis, SDS-PAGE, MALDI-TOF Mass spectroscopy and Western blot. The SDS-PAGE and 2D gel analysis revealed that rohu native leptin possesses molecular mass of 16 kDa. Western blot analysis showed that the fish hepatocytes possessed the sero-reactive leptin protein of 16 kDa. MALDI-TOF mass spectroscopy and peptide analysis showed the molecular mass of rohu leptin as 16283.38 Da. The serodiagnostic potential of native leptin of rohu was revealed for the first time while assessing its serological responses by ELISA using anti-leptin antibodies.展开更多
Fry rearing is one of the important stage which aims at obtaining high growth and survival for production of fingerlings required for stocking into grow out ponds as well as rehabilitation in natural habitat. This exp...Fry rearing is one of the important stage which aims at obtaining high growth and survival for production of fingerlings required for stocking into grow out ponds as well as rehabilitation in natural habitat. This experiment was conducted with the purpose to test the effect of dietary crude protein level (CP %) of feeds prepared from similar feed ingredients in different ratios on growth performance and survival rate of Rohu fry (Labeorohita). An initial density of 100 fry/m2 was maintained in hapa fixed in the cemented tank. The dietary CP% level of feed tested were 20% CP, 25% CP, 30% CP, and 35% CP fed at 5% body weight. The experiment ran for 53 nursing days. The results showed that there was no significant difference (P > 0.05) in the growth rate (g/day) of fry among treatments. Rather group fed with higher protein level grew comparatively better indicating possibility of increasing need of protein in diets. However, the survival rate (%) of rohu fry was significantly different (P < 0.05) in each tested CP% level of feed. Highest survival (82%) of fry was found in the feed of CP 35% and lowest (56%) in the feed of CP 25%. It was predicted that feed with increasing level of CP % in diet is essential for increasing survival rate.展开更多
The purpose of this study was to evaluate the effect of fish collagen peptide(FCP)on improving the skin barrier against chronological aging in 12-month-old hairless mice.From the 9th month,including the age of purchas...The purpose of this study was to evaluate the effect of fish collagen peptide(FCP)on improving the skin barrier against chronological aging in 12-month-old hairless mice.From the 9th month,including the age of purchase and the adaptation period,mice were orally administered each treatment for 3 months.The experimental groups included young control(YC,8-week-old),aged control(CON,12-month-old),low-dose FCP(FCP-L,300 mg/kg FCP,12-month-old),and high-dose FCP(FCP-H,600 mg/kg FCP,12-month-old).FCP administration showed significant improvements in skin hydration(p<0.001)and wrinkle indices(p<0.001)and reduced transepidermal water loss(p<0.05)due to chronological aging.In addition,FCP administration significantly increased the expression of skin barrier-related genes and upregulated TGF-β/Smad signaling proteins.The expression of collagen homeostasis-related factors was also significantly improved in the FCP-H group.Furthermore,FCP reduced oxidative stress by enhancing the expression of antioxidant enzymes.LDA scores indicated variations in the gut microbiota among groups,with YC(28),CON(8),FCP-L(6),and FCP-H(21).The abundance of Bacteroidetes and Firmicutes was significantly reversed in the CON group compared to the YC group.At the genus level,the YC had increased levels of Pseudobutyrivibrio sp.,Acetivibrio sp.,Phocea sp.and Ruminococcus sp.Relative to the CON.Notably,the FCP-H showed elevated levels of Adlercreutzia sp.compared to the CON(p<0.05).Overall,FCP administration effectively mitigated skin barrier damage caused by chronological aging,regulated key factors involved in skin aging,and contributed to the maintenance of youthful skin,thereby delaying the natural aging process.展开更多
Background: The homeobox containing transcription factor Nanog plays crucial roles in embryonic development/proliferation and/or maintenance of spermatogonial stem cells(SSCs) via interacting with transcription factor...Background: The homeobox containing transcription factor Nanog plays crucial roles in embryonic development/proliferation and/or maintenance of spermatogonial stem cells(SSCs) via interacting with transcription factors such as Oct4 and Sox2 in mammals. However, knowledge of its exact mechanistic pathways remains unexploited. Very little is known about teleost Nanog. Information on the Nanog gene of farmed rohu carp(Labeo rohita) is lacking. We cloned and characterized the Nanog gene of rohu carp to understand the expression pattern in early developmental stages and also deduced the genomic organization including promoter elements.Results: Rohu Nanog(LrNanog) cDNA comprised an open reading frame of 1,161 nucleotides bearing a structural homeodomain; whereas, the genomic structure contained four exons and three introns suggesting that it is homologous to mammalian counterparts. Phylogenetical y, it was closely related to freshwater counterparts. Protein sequence(386 AA of42.65 kDa) comparison revealed its low similarity with other vertebrate counterparts except that of the conserved homeodomain. Tissue distribution analysis revealed the existence of LrNanog transcripts only in adult gonads. The heightened abundances in the ovary and proliferating spermatogonia suggested its participations in maternal inheritance and male germ cell development. The potentiating abundances from fertilized egg onwards peaking at blastula stage vis-à-vis decreasing levels from gastrula stage onwards demonstrated its role in embryonic stem cell development. We also provided evidence of its presence in SSCs by western blotting analysis. Further, the promoter region was characterized, predicting a basal core promoter and other consensus elements.Conclusion: The molecular characterization of LrNanog and its documented expression profiling at transcript and protein levels are indicative of its functional linkage with embryonic/spermatogonial stem cell maintenance. This is the first report of LrNanog genomic organization including its promoter sequence information with predicted regulatory elements of a large-bodied carp species. This will be useful for elucidating its mechanism expression in future. Nanog could be used as a potential biomarker for proliferating carp SSCs.展开更多
文摘The gel-forming ability of rohu (Labeo rohita) mince with and without egg white powder (EW) was investigated. Gel from washed mince (washed gel) was prepared under two setting conditions: kamaboko (40°C) and modori (60°C). The gel-forming ability of kamaboko and modori gels was improved by the addition of EW at 2%. The autolytic inhibition of kamaboko gel was obtained in gel added with 2% EW, and 1% EW of modori gel. No marked change was observed in the TCA-soluble peptide content of either gel with the addition of EW above 1%. No effect on the whiteness of both gels was shown after the addition of EW. The addition of EW exhibited smaller cavities and a more compact fibrous network in microstructure.
文摘Information regarding molecular characteristics of leptin protein in different animal species in-cluding fish is scarce. With the aim of characterizing the native leptin protein of Indian major carprohu (Labeo rohita), at molecular level, the present study was designed to isolate rohu leptin from its hepatocytes (the prime source of leptin in fish) and immunobiochemical characterization of the same, subsequently. In the present study, chemical treatment and ultra-sonication technique was used for isolating leptin from rohu liver tissue. Purification of the protein was attempted using affinity column chromatography. The molecular, biophysical and serological characterization of rohu leptin was carried out by 2D-gel electrophoreis, SDS-PAGE, MALDI-TOF Mass spectroscopy and Western blot. The SDS-PAGE and 2D gel analysis revealed that rohu native leptin possesses molecular mass of 16 kDa. Western blot analysis showed that the fish hepatocytes possessed the sero-reactive leptin protein of 16 kDa. MALDI-TOF mass spectroscopy and peptide analysis showed the molecular mass of rohu leptin as 16283.38 Da. The serodiagnostic potential of native leptin of rohu was revealed for the first time while assessing its serological responses by ELISA using anti-leptin antibodies.
文摘Fry rearing is one of the important stage which aims at obtaining high growth and survival for production of fingerlings required for stocking into grow out ponds as well as rehabilitation in natural habitat. This experiment was conducted with the purpose to test the effect of dietary crude protein level (CP %) of feeds prepared from similar feed ingredients in different ratios on growth performance and survival rate of Rohu fry (Labeorohita). An initial density of 100 fry/m2 was maintained in hapa fixed in the cemented tank. The dietary CP% level of feed tested were 20% CP, 25% CP, 30% CP, and 35% CP fed at 5% body weight. The experiment ran for 53 nursing days. The results showed that there was no significant difference (P > 0.05) in the growth rate (g/day) of fry among treatments. Rather group fed with higher protein level grew comparatively better indicating possibility of increasing need of protein in diets. However, the survival rate (%) of rohu fry was significantly different (P < 0.05) in each tested CP% level of feed. Highest survival (82%) of fry was found in the feed of CP 35% and lowest (56%) in the feed of CP 25%. It was predicted that feed with increasing level of CP % in diet is essential for increasing survival rate.
基金supported by CKD Healthcare(Seoul,Republic of Korea).
文摘The purpose of this study was to evaluate the effect of fish collagen peptide(FCP)on improving the skin barrier against chronological aging in 12-month-old hairless mice.From the 9th month,including the age of purchase and the adaptation period,mice were orally administered each treatment for 3 months.The experimental groups included young control(YC,8-week-old),aged control(CON,12-month-old),low-dose FCP(FCP-L,300 mg/kg FCP,12-month-old),and high-dose FCP(FCP-H,600 mg/kg FCP,12-month-old).FCP administration showed significant improvements in skin hydration(p<0.001)and wrinkle indices(p<0.001)and reduced transepidermal water loss(p<0.05)due to chronological aging.In addition,FCP administration significantly increased the expression of skin barrier-related genes and upregulated TGF-β/Smad signaling proteins.The expression of collagen homeostasis-related factors was also significantly improved in the FCP-H group.Furthermore,FCP reduced oxidative stress by enhancing the expression of antioxidant enzymes.LDA scores indicated variations in the gut microbiota among groups,with YC(28),CON(8),FCP-L(6),and FCP-H(21).The abundance of Bacteroidetes and Firmicutes was significantly reversed in the CON group compared to the YC group.At the genus level,the YC had increased levels of Pseudobutyrivibrio sp.,Acetivibrio sp.,Phocea sp.and Ruminococcus sp.Relative to the CON.Notably,the FCP-H showed elevated levels of Adlercreutzia sp.compared to the CON(p<0.05).Overall,FCP administration effectively mitigated skin barrier damage caused by chronological aging,regulated key factors involved in skin aging,and contributed to the maintenance of youthful skin,thereby delaying the natural aging process.
基金provided by grant from Indian Council of Agricultural Research (ICAR) and Department of Biotechnology(DBT),Government of India
文摘Background: The homeobox containing transcription factor Nanog plays crucial roles in embryonic development/proliferation and/or maintenance of spermatogonial stem cells(SSCs) via interacting with transcription factors such as Oct4 and Sox2 in mammals. However, knowledge of its exact mechanistic pathways remains unexploited. Very little is known about teleost Nanog. Information on the Nanog gene of farmed rohu carp(Labeo rohita) is lacking. We cloned and characterized the Nanog gene of rohu carp to understand the expression pattern in early developmental stages and also deduced the genomic organization including promoter elements.Results: Rohu Nanog(LrNanog) cDNA comprised an open reading frame of 1,161 nucleotides bearing a structural homeodomain; whereas, the genomic structure contained four exons and three introns suggesting that it is homologous to mammalian counterparts. Phylogenetical y, it was closely related to freshwater counterparts. Protein sequence(386 AA of42.65 kDa) comparison revealed its low similarity with other vertebrate counterparts except that of the conserved homeodomain. Tissue distribution analysis revealed the existence of LrNanog transcripts only in adult gonads. The heightened abundances in the ovary and proliferating spermatogonia suggested its participations in maternal inheritance and male germ cell development. The potentiating abundances from fertilized egg onwards peaking at blastula stage vis-à-vis decreasing levels from gastrula stage onwards demonstrated its role in embryonic stem cell development. We also provided evidence of its presence in SSCs by western blotting analysis. Further, the promoter region was characterized, predicting a basal core promoter and other consensus elements.Conclusion: The molecular characterization of LrNanog and its documented expression profiling at transcript and protein levels are indicative of its functional linkage with embryonic/spermatogonial stem cell maintenance. This is the first report of LrNanog genomic organization including its promoter sequence information with predicted regulatory elements of a large-bodied carp species. This will be useful for elucidating its mechanism expression in future. Nanog could be used as a potential biomarker for proliferating carp SSCs.
