Histogenesis of the immune system and specific activity of superoxide dismutase(SOD) were studied in rock bream Oplegnathus fasciatus from fertilization to 50 days after hatching(DAH).The pronephric tubule primordium ...Histogenesis of the immune system and specific activity of superoxide dismutase(SOD) were studied in rock bream Oplegnathus fasciatus from fertilization to 50 days after hatching(DAH).The pronephric tubule primordium developed in the embryo,14 h 30 min post fertilization.The spleen anlage was observed between the swim bladder and the intestine at 5 DAH,and the thymus was formed as a paired structure under the pharyngeal epithelium above the gill arch at 10 DAH.The order of the immune organs becoming lymphoid was the pronephric kidney(10 DAH),thymus(15 DAH) and spleen(21 DAH).As the embryo developed,the specific activity of SOD gradually increased until hatching,but subsequently SOD activity continuously decreased to a minimum at 14 DAH.After the spleen became lymphoid,the specific activity of SOD was relatively stable.It is suggested that the immaturity of the lymphoid organs and low specific activity of SOD was the cause of the high mortality of fingerlings 12 to 16 DAH.展开更多
Rock bream (Oplegnathus fasciatus) is one of the most economically valuable fish in Korea. In recent years, artificial breeding techniques with molecular and microsatellite markers have been developed to enhance rock ...Rock bream (Oplegnathus fasciatus) is one of the most economically valuable fish in Korea. In recent years, artificial breeding techniques with molecular and microsatellite markers have been developed to enhance rock bream resources. Microsatellite loci to define genetic diversity were screened in rock bream (n = 30) from Jeju areas of Korea and fifteen polymorphic microsatellite loci were newly identified and analyzed. The number of alleles per locus ranged from 9 to 34 while observed and expected heterozygosity ranged from 0.600 to 1.000 and from 0.772 to 0.977, respectively. These markers will serve as a foundation for future population genetic studies and the selective breeding technology of rock bream farming.展开更多
基金Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A413-1)
文摘Histogenesis of the immune system and specific activity of superoxide dismutase(SOD) were studied in rock bream Oplegnathus fasciatus from fertilization to 50 days after hatching(DAH).The pronephric tubule primordium developed in the embryo,14 h 30 min post fertilization.The spleen anlage was observed between the swim bladder and the intestine at 5 DAH,and the thymus was formed as a paired structure under the pharyngeal epithelium above the gill arch at 10 DAH.The order of the immune organs becoming lymphoid was the pronephric kidney(10 DAH),thymus(15 DAH) and spleen(21 DAH).As the embryo developed,the specific activity of SOD gradually increased until hatching,but subsequently SOD activity continuously decreased to a minimum at 14 DAH.After the spleen became lymphoid,the specific activity of SOD was relatively stable.It is suggested that the immaturity of the lymphoid organs and low specific activity of SOD was the cause of the high mortality of fingerlings 12 to 16 DAH.
文摘Rock bream (Oplegnathus fasciatus) is one of the most economically valuable fish in Korea. In recent years, artificial breeding techniques with molecular and microsatellite markers have been developed to enhance rock bream resources. Microsatellite loci to define genetic diversity were screened in rock bream (n = 30) from Jeju areas of Korea and fifteen polymorphic microsatellite loci were newly identified and analyzed. The number of alleles per locus ranged from 9 to 34 while observed and expected heterozygosity ranged from 0.600 to 1.000 and from 0.772 to 0.977, respectively. These markers will serve as a foundation for future population genetic studies and the selective breeding technology of rock bream farming.
