The viral protein 22(VP22)in the tegument of Marek’s disease virus serotype 1(MDV-1)plays an im-portant role in cell-to-cell spread and viral propagation.Antiserum against the carboxyl terminus of VP22 was prepared b...The viral protein 22(VP22)in the tegument of Marek’s disease virus serotype 1(MDV-1)plays an im-portant role in cell-to-cell spread and viral propagation.Antiserum against the carboxyl terminus of VP22 was prepared by immunizing mice with recombinant VP22 expressed in E.coli,and used to in-vestigate its expression in chicken embryo fibroblast(CEF)cells infected with different MDV-1 strains.At an infection dose of PFU=50,intercellular trafficking of the VP22 into the nuclei of the surrounding receipt cells was detected as early as 3 hours post infection.By 6 hours after infection(before viral plague formation),the protein was detected in the whole nuclei of the recipient cells with no difference among MDV-1 strains CVI988/Rispens,GA and RB1B.Intra-nuclear accumulation of the VP22 protein was further increased when the viral plagues started to form.These results indicate that,albeit the ex-istence of the 201TKSERT206 deletion,the VP22 of the CVI988/Rispens vaccine strain has also intercel-lular-trafficking function,which might serve as a potential alternative delivering protein instead of virulent strains VP22.展开更多
基金the National Natural Science Foundation of China(Grant No.30371070)
文摘The viral protein 22(VP22)in the tegument of Marek’s disease virus serotype 1(MDV-1)plays an im-portant role in cell-to-cell spread and viral propagation.Antiserum against the carboxyl terminus of VP22 was prepared by immunizing mice with recombinant VP22 expressed in E.coli,and used to in-vestigate its expression in chicken embryo fibroblast(CEF)cells infected with different MDV-1 strains.At an infection dose of PFU=50,intercellular trafficking of the VP22 into the nuclei of the surrounding receipt cells was detected as early as 3 hours post infection.By 6 hours after infection(before viral plague formation),the protein was detected in the whole nuclei of the recipient cells with no difference among MDV-1 strains CVI988/Rispens,GA and RB1B.Intra-nuclear accumulation of the VP22 protein was further increased when the viral plagues started to form.These results indicate that,albeit the ex-istence of the 201TKSERT206 deletion,the VP22 of the CVI988/Rispens vaccine strain has also intercel-lular-trafficking function,which might serve as a potential alternative delivering protein instead of virulent strains VP22.
