Biological insecticides have gained importance in modern society because they cause minimal environmental harm and reduce pests’ability to develop resistance.Nomuraea rileyi,a facultative entomopathogenic fungus,is s...Biological insecticides have gained importance in modern society because they cause minimal environmental harm and reduce pests’ability to develop resistance.Nomuraea rileyi,a facultative entomopathogenic fungus,is suitable for infecting lepidopteran pests,including Spodoptera litura and Helicoverpa armigera.This work aims to identify a suitable semi-solid fermentation medium to increase the conidia production of N.rileyi and enhance its biological control ability as mycoinsecticide.Research is carried out on broken rice,wheat,sorghum,and corn as substrates,both with and without nutrient addition.It also covers incubation under set conditions,colony harvesting,and conidial yield and germination determination using a haemocytometer.All the study was conducted using a completely randomized design(CRD)and a test of significance was set at p<0.05 using analysis of variance(ANOVA).This work aimed to establish the effect of various substrates on growth and conidia formation in N.rileyi.The highest conidia count of 10×10^(8)CFU/g on day 15.The result was obtained from BR+Nu followed by sorghum 7×10^(8)CFU/g,corn 7×10^(8)CFU/g and wheat 6.5×10^(8)CFU/g.Thus,post-drying,the highest corresponding viability was observed in the product containing BR+Nu–6.20×10^(8)CFU/g.Adding rice bran(BR+RB,150 g each)still increases conidial yield up to 14×10^(8) CFU/g at 15 DAI.These indings thus assert that broken rice and supplementation affect conidia yield and viability.展开更多
文摘Biological insecticides have gained importance in modern society because they cause minimal environmental harm and reduce pests’ability to develop resistance.Nomuraea rileyi,a facultative entomopathogenic fungus,is suitable for infecting lepidopteran pests,including Spodoptera litura and Helicoverpa armigera.This work aims to identify a suitable semi-solid fermentation medium to increase the conidia production of N.rileyi and enhance its biological control ability as mycoinsecticide.Research is carried out on broken rice,wheat,sorghum,and corn as substrates,both with and without nutrient addition.It also covers incubation under set conditions,colony harvesting,and conidial yield and germination determination using a haemocytometer.All the study was conducted using a completely randomized design(CRD)and a test of significance was set at p<0.05 using analysis of variance(ANOVA).This work aimed to establish the effect of various substrates on growth and conidia formation in N.rileyi.The highest conidia count of 10×10^(8)CFU/g on day 15.The result was obtained from BR+Nu followed by sorghum 7×10^(8)CFU/g,corn 7×10^(8)CFU/g and wheat 6.5×10^(8)CFU/g.Thus,post-drying,the highest corresponding viability was observed in the product containing BR+Nu–6.20×10^(8)CFU/g.Adding rice bran(BR+RB,150 g each)still increases conidial yield up to 14×10^(8) CFU/g at 15 DAI.These indings thus assert that broken rice and supplementation affect conidia yield and viability.
