Background Rickettsia and related diseases have been identified as significant global public health threats.This study involved comprehensive field and systematic investigations of various rickettsial organisms in Yun...Background Rickettsia and related diseases have been identified as significant global public health threats.This study involved comprehensive field and systematic investigations of various rickettsial organisms in Yunnan Province.Methods Between May 18,2011 and November 23,2020,field investigations were conducted across 42 counties in Yunnan Province,China,encompassing small mammals,livestock,and ticks.Preliminary screenings for Rickettsiales involved amplifying the 16S rRNA genes,along with additional genus-or species-specific genes,which were subse-quently confrmed through sequencing results.Sequence comparisons were carried out using the Basic Local Align-ment Search Tool(BLAST).Phylogenetic relationships were analyzed using the default parameters in the Molecular Evolutionary Genetics Analysis(MEGA)program.The chi-squared test was used to assess the diversities and compo-nent ratios of rickettsial agents across various parameters.Results A total of 7964 samples were collected from small mammals,livestock,and ticks through Yunnan Province and submitted for screening for rickettsial organisms.Sixteen rickettsial species from the genera Rickettsia,Anaplasma,Ehrlichia,Neoehrlichia,and Wolbachia were detected,with an overall prevalence of 14.72%.Among these,11 species were identifed as pathogens or potential pathogens to humans and livestock.Specifically,10 rickettsial organisms were widely found in 42.11%(24 out of 57)of small mammal species.High prevalence was observed in Dremomys samples at 5.60%,in samples from regions with latitudes above 4000 m or alpine meadows,and in those obtained from Yuanmou County.Anaplasma phagocytophilum and Candidatus Neoehrlichia mikurensis were broadly infect-ing multiple genera of animal hosts.In contrast,the small mammal genera Neodon,Dremomys,Ochotona,Anouro-sorex,and Mus were carrying individually specific rickettsial agents,indicating host tropism.There were 13 rickettsial species detected in 57.14%(8 out of 14)of tick species,with the highest prevalence(37.07%)observed in the genus Rhipicephalus.Eight rickettsial species were identified in 2375 livestock samples.Notably,six new Rickettsiales variants/strains were discovered,and Candidatus Rickettsia longicorni was unambiguously identified.Conclusions This large-scale survey provided further insight into the high genetic diversity and overall prevalence of emerging Rickettsiales within endemic hotspots in Yunnan Province.The potential threats posed by these emerging tick-borne Rickettsiales to public health warrant attention,underscoring the need for effective strategies to guide the prevention and control of emerging zoonotic diseases in China.展开更多
Pathogens like bacteria and protozoa,which affect human and animal health worldwide,can be transmitted by vectors like ticks.To investigate the epidemiology and genetic diversity of bacteria and protozoans carried by ...Pathogens like bacteria and protozoa,which affect human and animal health worldwide,can be transmitted by vectors like ticks.To investigate the epidemiology and genetic diversity of bacteria and protozoans carried by ticks in Chengmai county of Hainan province,China,285 adult hard ticks belonging to two species[Rhipicephalus sanguineus(sensu lato):183,64.21%and Rhipicephalus microplus:102,35.79%]from dogs,cattle,and goats were collected.Microbial families were identified in these ticks by amplifying the 18 S r RNA,16 S r RNA(rrs),citrate synthase(glt A),and heat shock protein(gro EL)genes.Our data revealed the presence of four recognized species and two Candidatus spp.of Anaplasmataceae and Coxiellaceae.In sum,these data reveal an extensive diversity of Anaplasmataceae bacteria,Coxiellaceae bacteria,Babesiidae,and Hepatozoidae in ticks from Hainan Island,highlighting the need to understand the tickborne pathogen infection in local animals and humans.展开更多
Background:In this study,we present a case of Japanese spotted fever(JSF)caused by Rickettsia japonica and use this case to investigate the process of diagnosing and reintegrating traceability of infectious diseases v...Background:In this study,we present a case of Japanese spotted fever(JSF)caused by Rickettsia japonica and use this case to investigate the process of diagnosing and reintegrating traceability of infectious diseases via metagenomic next-generation sequencing(mNGS).Methods:From data relating to epidemiological history,clinical and laboratory examinations,and mNGS se-quencing,a diagnosis of severe JSF was concluded.Results:A detailed field epidemiological investigation discovered parasitic Haemaphysalis longicornis from a host animal(dog)in the domicile of the patient,within which R.japonica was detected,along with a diverse array of other potentially pathogenic microorganisms that could cause other infectious diseases.Conclusion:The mNGS provided an efficient method to diagnose JSF infection.This methodology could also be applied to field epidemiological investigations to establish the traceability of infectious diseases.展开更多
文摘Background Rickettsia and related diseases have been identified as significant global public health threats.This study involved comprehensive field and systematic investigations of various rickettsial organisms in Yunnan Province.Methods Between May 18,2011 and November 23,2020,field investigations were conducted across 42 counties in Yunnan Province,China,encompassing small mammals,livestock,and ticks.Preliminary screenings for Rickettsiales involved amplifying the 16S rRNA genes,along with additional genus-or species-specific genes,which were subse-quently confrmed through sequencing results.Sequence comparisons were carried out using the Basic Local Align-ment Search Tool(BLAST).Phylogenetic relationships were analyzed using the default parameters in the Molecular Evolutionary Genetics Analysis(MEGA)program.The chi-squared test was used to assess the diversities and compo-nent ratios of rickettsial agents across various parameters.Results A total of 7964 samples were collected from small mammals,livestock,and ticks through Yunnan Province and submitted for screening for rickettsial organisms.Sixteen rickettsial species from the genera Rickettsia,Anaplasma,Ehrlichia,Neoehrlichia,and Wolbachia were detected,with an overall prevalence of 14.72%.Among these,11 species were identifed as pathogens or potential pathogens to humans and livestock.Specifically,10 rickettsial organisms were widely found in 42.11%(24 out of 57)of small mammal species.High prevalence was observed in Dremomys samples at 5.60%,in samples from regions with latitudes above 4000 m or alpine meadows,and in those obtained from Yuanmou County.Anaplasma phagocytophilum and Candidatus Neoehrlichia mikurensis were broadly infect-ing multiple genera of animal hosts.In contrast,the small mammal genera Neodon,Dremomys,Ochotona,Anouro-sorex,and Mus were carrying individually specific rickettsial agents,indicating host tropism.There were 13 rickettsial species detected in 57.14%(8 out of 14)of tick species,with the highest prevalence(37.07%)observed in the genus Rhipicephalus.Eight rickettsial species were identified in 2375 livestock samples.Notably,six new Rickettsiales variants/strains were discovered,and Candidatus Rickettsia longicorni was unambiguously identified.Conclusions This large-scale survey provided further insight into the high genetic diversity and overall prevalence of emerging Rickettsiales within endemic hotspots in Yunnan Province.The potential threats posed by these emerging tick-borne Rickettsiales to public health warrant attention,underscoring the need for effective strategies to guide the prevention and control of emerging zoonotic diseases in China.
基金The National Science and Technology Major Project of China[No.2018ZX10101002-002]the National Natural Science Foundation of China[grants 81702016]the National Science and Technology Major Project of China[2018ZX10712001-006-002 and 2018ZX10305409-003-005]。
文摘Pathogens like bacteria and protozoa,which affect human and animal health worldwide,can be transmitted by vectors like ticks.To investigate the epidemiology and genetic diversity of bacteria and protozoans carried by ticks in Chengmai county of Hainan province,China,285 adult hard ticks belonging to two species[Rhipicephalus sanguineus(sensu lato):183,64.21%and Rhipicephalus microplus:102,35.79%]from dogs,cattle,and goats were collected.Microbial families were identified in these ticks by amplifying the 18 S r RNA,16 S r RNA(rrs),citrate synthase(glt A),and heat shock protein(gro EL)genes.Our data revealed the presence of four recognized species and two Candidatus spp.of Anaplasmataceae and Coxiellaceae.In sum,these data reveal an extensive diversity of Anaplasmataceae bacteria,Coxiellaceae bacteria,Babesiidae,and Hepatozoidae in ticks from Hainan Island,highlighting the need to understand the tickborne pathogen infection in local animals and humans.
基金supported by the National Natural Science Foundation of China(82273689)Key Research and Development Program of Shaanxi(2024SF-YBXM-289).
文摘Background:In this study,we present a case of Japanese spotted fever(JSF)caused by Rickettsia japonica and use this case to investigate the process of diagnosing and reintegrating traceability of infectious diseases via metagenomic next-generation sequencing(mNGS).Methods:From data relating to epidemiological history,clinical and laboratory examinations,and mNGS se-quencing,a diagnosis of severe JSF was concluded.Results:A detailed field epidemiological investigation discovered parasitic Haemaphysalis longicornis from a host animal(dog)in the domicile of the patient,within which R.japonica was detected,along with a diverse array of other potentially pathogenic microorganisms that could cause other infectious diseases.Conclusion:The mNGS provided an efficient method to diagnose JSF infection.This methodology could also be applied to field epidemiological investigations to establish the traceability of infectious diseases.
