We collected infection plants of rice bakanae desiease from 22 counties (cities) in Hubei Province in 1990-1992. Thirty five isolates of single spore were isolated from the plants. Fusarium species were identified fro...We collected infection plants of rice bakanae desiease from 22 counties (cities) in Hubei Province in 1990-1992. Thirty five isolates of single spore were isolated from the plants. Fusarium species were identified from these isolates based on the method of Booth(1971) and other authors. Six kinds of medium wereused in the experiment. Color and growth rate ofcolonies as well as other characters were determinedfrom cultures grown on PSA. The results showedthat thirty one isolates were Fusarium moniliformevar. zhejiangensis, two isolates were Fusariumgramineum, one was Fusariurn oxysporum, and onewas Fusarium solani.展开更多
Enhancement of nitrogen fixation in the rhizo-sphere of cereals has attracted a wide interestin biological and agricultural research,insteadof chemicals,for supplying higher plants withcombined nitrogen.Bacteria in as...Enhancement of nitrogen fixation in the rhizo-sphere of cereals has attracted a wide interestin biological and agricultural research,insteadof chemicals,for supplying higher plants withcombined nitrogen.Bacteria in association withrice plant.s were sensitive to the surroundingfactors in the soil,such as NH~+ or O,whichrepressed associative nitrogen fixation between展开更多
A set of near-isogenic rice lines withmonogenic resistance to bacterial blight weredeveloped by IRRI.The Cultivar IR24 wasused as the recurred parent.They wereevaluated with 6 races of Xanthomonascampestris pv.oryzae(...A set of near-isogenic rice lines withmonogenic resistance to bacterial blight weredeveloped by IRRI.The Cultivar IR24 wasused as the recurred parent.They wereevaluated with 6 races of Xanthomonascampestris pv.oryzae(Xco)in the Philip-pines at the maximum tillering and the bootingstages by ZHANG and MEW at IRRI in 1989.展开更多
[Objective] The paper was to study the expression characteristics and borer resistance of Bt gene of Songjing 9 （1C^＊）. [Method] With super japonica rice Songjing 9 as the receptor, transgenic crylC^＊ super japon...[Objective] The paper was to study the expression characteristics and borer resistance of Bt gene of Songjing 9 （1C^＊）. [Method] With super japonica rice Songjing 9 as the receptor, transgenic crylC^＊ super japonica rice Songjing 9 （1C^＊） regulated by ubi promoter was created by agrobacterium-mediated genetic transformation method. Ten strains of transgenic super japonica rice formed from different transformation events were planted in the field; the Tt-mRNA of leaf, stem-sheath and young spikelet at booting stage were detected by real-time fluorescent quantitative PCR; the Bt protein content of leaf, stem-sheath and young spikelet and harvested brown rice were detected by enzyme-linked immunosorbent assay （ELISA）; the white head rate damaged by Chilo suppressalis was manually investigated. [Result] The Bt-mRNA and Bt protein content of various organs and Bt protein content of mature brown rice at booting stage of Songjing 9 （1C^＊） significantly differed among strains; there was no correlation relationship in Bt-mRNA and Bt protein content between leaf and stem-sheath, between leaf and young spikelet, and between stem-sheath and young spikelet; there was no relation between Bt-mRNA or Bt protein content of leaf, stem-sheath and young spikelet and Bt protein content of brown rice; there was positive correlation between Bt-mRNA and Bt protein content in the same organ such as leaf, stem-sheath and young spikelet at booting stage; such strains as Songjing 9 （1C^＊）, whether the Bt gene expression was high or low, showed good resistance against C.suppressalis. This indicated that there were differences in Bt gene expression in different strains of transgenic cry1C^＊ super japonica rice or different organs of the same strain; there was no relation in Bt gene expression among different organs; the Bt-mRNA and protein content of the same organ usually had consistent expression; the Bt gene expression of various organs had nothing to do with the Bt protein content of brown rice. [Conclusion] The Bt gene and its expression cassette can meet the requirement of cultivating japonica rice varieties against stem borers.展开更多
The hardness of cooked rice is one of the most important criteria which determine the rice quality. The commonly used near-infrared reflectance (NIR) method is still in argument due to its indirectness and possible er...The hardness of cooked rice is one of the most important criteria which determine the rice quality. The commonly used near-infrared reflectance (NIR) method is still in argument due to its indirectness and possible error. In this paper, a mechanical method was proposed and its principle, automation, components and operative reliability were evaluated and compared with the NIR method. The results showed that the mechanical testing method can accurately detect the rice quality and were consistent with the NIR testing data. This new mechanical method can be effectively used in rice quality testing and branding with the advantage of simplicity, accuracy and reliability.展开更多
文摘We collected infection plants of rice bakanae desiease from 22 counties (cities) in Hubei Province in 1990-1992. Thirty five isolates of single spore were isolated from the plants. Fusarium species were identified from these isolates based on the method of Booth(1971) and other authors. Six kinds of medium wereused in the experiment. Color and growth rate ofcolonies as well as other characters were determinedfrom cultures grown on PSA. The results showedthat thirty one isolates were Fusarium moniliformevar. zhejiangensis, two isolates were Fusariumgramineum, one was Fusariurn oxysporum, and onewas Fusarium solani.
文摘Enhancement of nitrogen fixation in the rhizo-sphere of cereals has attracted a wide interestin biological and agricultural research,insteadof chemicals,for supplying higher plants withcombined nitrogen.Bacteria in association withrice plant.s were sensitive to the surroundingfactors in the soil,such as NH~+ or O,whichrepressed associative nitrogen fixation between
文摘A set of near-isogenic rice lines withmonogenic resistance to bacterial blight weredeveloped by IRRI.The Cultivar IR24 wasused as the recurred parent.They wereevaluated with 6 races of Xanthomonascampestris pv.oryzae(Xco)in the Philip-pines at the maximum tillering and the bootingstages by ZHANG and MEW at IRRI in 1989.
基金Supported by National High Technology Research and Development Program(863 plan)of China(2014AA10A600)National Major Project for Transgenic Organism Breeding of China(2016ZX08001001-001-007)Open Project of Key Laboratory of Molecular Biology in Colleges and Universities of Heilongjiang Province(1206)
文摘[Objective] The paper was to study the expression characteristics and borer resistance of Bt gene of Songjing 9 （1C^＊）. [Method] With super japonica rice Songjing 9 as the receptor, transgenic crylC^＊ super japonica rice Songjing 9 （1C^＊） regulated by ubi promoter was created by agrobacterium-mediated genetic transformation method. Ten strains of transgenic super japonica rice formed from different transformation events were planted in the field; the Tt-mRNA of leaf, stem-sheath and young spikelet at booting stage were detected by real-time fluorescent quantitative PCR; the Bt protein content of leaf, stem-sheath and young spikelet and harvested brown rice were detected by enzyme-linked immunosorbent assay （ELISA）; the white head rate damaged by Chilo suppressalis was manually investigated. [Result] The Bt-mRNA and Bt protein content of various organs and Bt protein content of mature brown rice at booting stage of Songjing 9 （1C^＊） significantly differed among strains; there was no correlation relationship in Bt-mRNA and Bt protein content between leaf and stem-sheath, between leaf and young spikelet, and between stem-sheath and young spikelet; there was no relation between Bt-mRNA or Bt protein content of leaf, stem-sheath and young spikelet and Bt protein content of brown rice; there was positive correlation between Bt-mRNA and Bt protein content in the same organ such as leaf, stem-sheath and young spikelet at booting stage; such strains as Songjing 9 （1C^＊）, whether the Bt gene expression was high or low, showed good resistance against C.suppressalis. This indicated that there were differences in Bt gene expression in different strains of transgenic cry1C^＊ super japonica rice or different organs of the same strain; there was no relation in Bt gene expression among different organs; the Bt-mRNA and protein content of the same organ usually had consistent expression; the Bt gene expression of various organs had nothing to do with the Bt protein content of brown rice. [Conclusion] The Bt gene and its expression cassette can meet the requirement of cultivating japonica rice varieties against stem borers.
文摘The hardness of cooked rice is one of the most important criteria which determine the rice quality. The commonly used near-infrared reflectance (NIR) method is still in argument due to its indirectness and possible error. In this paper, a mechanical method was proposed and its principle, automation, components and operative reliability were evaluated and compared with the NIR method. The results showed that the mechanical testing method can accurately detect the rice quality and were consistent with the NIR testing data. This new mechanical method can be effectively used in rice quality testing and branding with the advantage of simplicity, accuracy and reliability.
