The rice ratooning system has attracted increasing attention in southern China due to its low carbon emissions and high yield potential.However,the net carbon budget and underlying mechanisms remain unclear.Three rice...The rice ratooning system has attracted increasing attention in southern China due to its low carbon emissions and high yield potential.However,the net carbon budget and underlying mechanisms remain unclear.Three rice cropping systems were established in this trial experiment conducted from 2021 to 2022 in Fuzhou(25°05'N,119°13'E),Southeast China:ratooning rice(RR:MC+RSR)pattern for rice ratooning,single-cropping rice(LR_(1)),and double-cropping rice(DC:ER+LR_(2)).The closed static dark box gas collection,dry matter determination,life cycle assessment(LCA)etc.approaches were utilized to investigate the mechanism of“high carbon fixation–low emissions”mechanism in RR.A comprehensive assessment was conducted across multiple dimensions,including crop yield,greenhouse gas(GHG)emissions,carbon and nitrogen footprints,resource use efficiency,carbon sequestration capacity,and carbon budget balance.Results showed that the average daily yield of ratoon season rice(RSR)across RR treatments from 2021 to 2022 was 28.21–47.40%higher than that of the main crop(MC)and LR_(1),and the average daily yield of RR was 13.50–27.76%higher than DC.This yield advantage was attributed to a 32.32–39.26%increase in the allocation of^(13)C-labeled photosynthetic products(including non-structural carbohydrates,NSCs)to panicle organs,and a 21.77–43.51%reduction in allocation to underground roots and soil.Furthermore,the average daily global warming potential(GWP)was 16.44 kg CO_(2)-eq ha^(–1)for RR,24.99 kg CO_(2)-eq ha^(–1)for LR_(1),and 21.32 kg CO_(2)-eq ha^(–1)for DC.Specifically,the average daily GWP of ratoon rice was 34.21%lower than that of LR_(1) and 22.90%lower than double-cropping rice.Similarly,the average daily greenhouse gas intensity(GHGI)of ratoon rice was 62.28%lower than LR_(1) and 28.96%lower than double-cropping rice.In terms of carbon and nitrogen footprints,the ratoon rice system exhibited average daily values of 34.54 kg CO_(2)-eq ha^(–1)and 0.47 kg N ha^(–1),respectively.In comparison,LR_(1) had values of 45.63 kg CO_(2)-eq ha^(–1)and 0.49 kg N ha^(–1),while double-cropping rice showed 43.38 kg CO_(2)-eq ha^(–1)and 0.53 kg N ha^(–1).These values represent reductions of 24.30%in carbon footprint and4.28%in nitrogen footprint relative to LR_(1),and 20.38 and 11.45%relative to double-cropping rice,respectively.Moreover,the average annual carbon budget surplus across systems was 22,380.01 kg CO_(2)-eq ha^(–1)for ratoon rice(MC+RSR),11,228.54 kg CO_(2)-eq ha^(–1)for LR_(1),and 23,772.15 kg CO_(2)-eq ha^(–1)for DC.Consequently,the resource utilization efficiency of the RR was 24.42 and 47.50%higher than that of single-cropping and double-cropping systems,respectively.Average daily economic returns also increased by 32.71 and 80.75%,respectively.These findings provide a robust theoretical foundation and practical guidance for advancing agricultural carbon neutrality technologies and ensuring food security.展开更多
The breakthrough in super hybrid rice yield has significantly contributed to China’s and global food security.However,the inherent conflict between high productivity and environmentally sustainable agriculture poses ...The breakthrough in super hybrid rice yield has significantly contributed to China’s and global food security.However,the inherent conflict between high productivity and environmentally sustainable agriculture poses substantial challenges.Issues such as water scarcity,energy crises,escalating greenhouse gas emissions,and diminishing farm profitability threaten longterm agricultural sustainability.In response,we applied a holistic food–carbon–nitrogen–water–energy–profit (FCNWEP)nexus framework to comprehensively assess the sustainability of distinct crop management strategies across three subsites in Central China.Field experiments were conducted in Hubei and Hunan provinces from 2017 to 2021 using a widely adopted elite super hybrid rice cultivar (Y-liangyou 900).Four crop management treatments were implemented:a control(CK,0 kg N ha^(-1)),conventional crop management (CCM,210–250 kg N ha^(-1),7:3 basal:mid-tiller fertilizer ratio),and two integrated crop management (ICM) treatments (ICM1,180–210 kg N ha^(-1),5:2:3 basal:mid-tiller:panicle initiation fertilizer ratio;ICM2,240–270 kg N ha^(-1),5:2:2:1 basal:mid-tiller:panicle initiation:flowering fertilizer ratio).Variables assessed included grain yield,carbon footprint,nitrogen footprint,water footprint,energy footprint,nitrogen use efficiency,and economic benefits.Our results showed significant yield variations,with ICM2 consistently outperforming CCM and ICM1across all three sites.In Jingzhou,Suizhou,and Changsha,ICM2’s grain yield was 30.2,24.7,and 13.3%higher than CCM,respectively.Net profits under ICM2 exceeded those of CCM and ICM1 by 31.8 and 115.2%in Jingzhou,32.2 and 109.9%in Suizhou,and 15.4 and 34.0%in Changsha,respectively.Integrated crop management,particularly ICM2,demonstrated improved nitrogen and energy use efficiency,leading to reduced carbon,nitrogen,water,and energy footprints.Overall,composite sustainability scores derived from the FCNWEP framework indicated that both ICM2 and ICM1 exhibited higher sustainability levels compared to CCM.This study provides valuable insights into practical management methodologies and offers recommendations for enhancing agricultural sustainability.展开更多
To examine the impact of anthropogenic land reconstruction,particularly the consolidation of small terraces into larger fields,on soil organic carbon(SOC),total nitrogen(TN)dynamics,rice yield,and its components,soil ...To examine the impact of anthropogenic land reconstruction,particularly the consolidation of small terraces into larger fields,on soil organic carbon(SOC),total nitrogen(TN)dynamics,rice yield,and its components,soil and plant samples were collected from seven newly reconstructed fields in Japanese Andosols in Tochigi,Japan.Samples were obtained from both the former low-and high-elevation sides within each field plot.During harvest season,nine rice plants were randomly selected from each plot(0.675 m^(2),comprising 3 rows and 3 hills per row),collected from a 3-m stretch along both the east(former low side)and west(former high side)ridges.Soil cores were collected from identical plots at two depths(0–15 and 15–30 cm)and combined into one composite sample per layer.Rice plant samples were air-dried for two weeks until reaching constant moisture content,after which stems and ears were separated and weighed to determine biomass,yield,yield components,and nitrogen uptake.This indicated that land reconstruction significantly affected rice yield and its components between the two sides of all field plots.SOC,TN,and their decomposition following land reconstruction showed notable changes,especially in the 15–30 cm subsurface soil layer.Additionally,grain weight demonstrated significant correlation with SOC,TN,and carbon decomposition in both the 0–15 and 15–30 cm layers,indicating that soil fertility to a depth of 30 cm was crucial for rice productivity after land reconstruction.展开更多
To elucidate the variations in volatile organic compounds(VOCs)among widely cultivated japonica rice varieties in China and offer novel perspectives on flavor formation during rice-based beer brewing,nine prevalent Ch...To elucidate the variations in volatile organic compounds(VOCs)among widely cultivated japonica rice varieties in China and offer novel perspectives on flavor formation during rice-based beer brewing,nine prevalent Chinese japonica rice variaties were selected as experimental materials.Comprehensive analyses were conducted to investigate three key aspects:differences in VOCs among the selected japonica rice varieties,the retention of rice VOCs after beer brewing,and the influence of rice additives on beer sensory characteristics.Results showed that the total contents of VOCs in Wuyoudao 4(WYD4)and Nangeng 9108(NG9108)were significantly higher than those in the other varieties(P<0.05).The popcorn-flavor compound 2-acetyl-1-pyrroline(2-AP)was detected in Suigeng 27(SG27,26.80 ng/g),WYD4(25.25 ng/g),NG9108(21.18 ng/g),and Suigeng 18(SG18,11.62 ng/g),with the levels in the first three varieties significantly higher than those in SG18.Circular heatmap analysis classified the nine japonica varieties into three major categories:fragrant japonica from North China(WYD4),fragrant japonica from South China(NG9108),and others.Ten characteristic VOCs across the japonica varieties were identified by headspace solid-phase microextraction gas chromatography-mass spectrometry(HS-SPME-GC-MS).Using beer brewed with whole wheat as the control,we analyzed the VOCs and sensory characteristics of beer brewed with fragrant japonica rice as an additive.We found that rice VOCs were not detected in beer brewed with fragrant japonica rice as an additive.Adding rice promoted the formation of banana-like flavors and reduced the beer’s richness and mellowness.展开更多
Photorespiration consumes photosynthetically fixed carbon and reduces yields by 20%–50%in C3 crops.In an attempt to increase photosynthetic efficiency in rice by bypassing the carbon-consuming process of photorespira...Photorespiration consumes photosynthetically fixed carbon and reduces yields by 20%–50%in C3 crops.In an attempt to increase photosynthetic efficiency in rice by bypassing the carbon-consuming process of photorespiration,a photorespiratory bypass consisting of Chlamydomonas reinhardtii glycolate dehydrogenase and Cucurbita maxima malate synthase(termed the GMS bypass)was introduced into the rice cultivar Zhonghua 11 and osplgg1b,a mutant of the rice chloroplast glycolate transporter,to generate GMS/ZH11 and GMS/osplgg1b transgenic plants.The GMS bypass reduced photorespiration and increased photosynthesis in the transgenic plants.The straw biomass of GMS/ZH11 and GMS/osplgg1b increased by up to 16.0%and 85.7%,respectively.The yield of GMS/ZH11 increased by 22.0%–34.7%in paddy fields.Thus,the GMS bypass can increase photosynthetic efficiency and yield in rice.展开更多
Chilo suppressalis(Walker)is one of the most important rice pests worldwide,posing a significant challenge to effective control.To develop a precision-timed,eco-friendly management strategy,overwintering population in...Chilo suppressalis(Walker)is one of the most important rice pests worldwide,posing a significant challenge to effective control.To develop a precision-timed,eco-friendly management strategy,overwintering population investigation and dynamic monitoring of C.suppressalis populations were conducted in the Meishan region of Sichuan,China,from 2023 to 2024.The optimal timing for insecticide application was estimated,followed by field trials evaluating the efficacy of different insecticides.Results demonstrated that the peak emergence of first-generation adults typically occurred in early July(under the environmental conditions of the Meishan region),with the ambient humidity below 75%and temperature around 29◦C.Pesticide efficacy trials show that insecticide combinations exhibited superior control.Notably,a combined treatment of emamectin benzoate⋅methoxyfenozide+chlorantraniliprole achieved the highest control efficacy(90.05%)and a corresponding yield of 12,491.55 kg/ha.All tested treatments were determined to be safe for rice growth.Furthermore,this optimized strategy resulted in notable economic benefits,including a 50%reduction in pesticide usage and cost savings of 4796.15 CNY compared to conventional practices.This study provides valuable insights into sustainable rice production and pest management and,for the first time,proposes a precision application time window based on intelligent monitoring.展开更多
Leaf thickness in rice critically influences photosynthetic efficiency and yield,yet its genetic basis remains poorly understood,with few functional genes previously characterized.In this study,we employ a pangenome-w...Leaf thickness in rice critically influences photosynthetic efficiency and yield,yet its genetic basis remains poorly understood,with few functional genes previously characterized.In this study,we employ a pangenome-wide association study(Pan-GWAS)on 302 diverse rice accessions from southern China,identifying 49 quantitative trait loci(QTLs)associated with leaf thickness.The most significant locus,qLT9,is fine-mapped to a 79-kb region on chromosome 9.Transcriptomic and genomic sequence analyses identify LOC_Os09g33480,which encodes a protein belonging to Multiple Organellar RNA Editing Factor family,as the key candidate gene.Overexpression and complementation transgenic experiments confirm LOC_Os09g33480(OsLT9)as the functional gene underlying qLT9,demonstrating a 24-bp Indel in its promoter correlates with the expression levels and leaf thickness.Notably,OsLT9 overexpression lines show not only thicker leaf,but also significantly enhanced photosynthetic efficiency and grain yield,establishing a link between leaf thickness modulation and yield enhancement.Population genomic analyses indicate strong selection for OsLT9 during domestication and breeding,with modern cultivars favoring thick leaf haplotype of OsLT9.This study establishes OsLT9 as a key regulator controlling leaf thickness in rice,and provides a valuable genetic resource for molecular breeding of high-yielding rice through optimization of plant architecture.展开更多
Accumulating evidence from recent studies has highlighted the critical regulatory functions of non-histone protein acetylation in rice biological processes.This review systematically synthesizes current advances in ch...Accumulating evidence from recent studies has highlighted the critical regulatory functions of non-histone protein acetylation in rice biological processes.This review systematically synthesizes current advances in characterizing the functional attributes and regulatory mechanisms of non-histone acetylation in rice,with a specific focus on its roles in regulating gene expression,modulating metabolic enzyme activities,and mediating stress responses.Emerging studies demonstrate that non-histone acetylation dynamically modulates transcription factors,metabolic enzymes,and other pivotal functional proteins to orchestrate essential physiological processes,including growth and development,photosynthetic efficiency,and environmental stress adaptation.Using mass spectrometry,gene editing,and related technologies,researchers have identified multiple acetyltransferases and deacetylases that regulate protein stability,subcellular localization,and protein-protein interactions.Despite these advances,challenges persist,such as the complexity of the acetylation regulatory networks and species-specific differences among cereal crops.Future investigations should integrate multi-omics approaches to elucidate the molecular mechanisms of this post-translational modification,thereby facilitating the development of targeted genetic engineering strategies for rice improvement.展开更多
Understanding Cd contamination in the soil-rice ecosystem and the underlying its threshold and interaction effects is crucial for controlling Cd pollution and ensuring food safety.Although the quantitative relationshi...Understanding Cd contamination in the soil-rice ecosystem and the underlying its threshold and interaction effects is crucial for controlling Cd pollution and ensuring food safety.Although the quantitative relationships between Cd and environmental variables have been extensively studied,the threshold and interaction effects of multi-source environmental variables remain largely unexplored.This study employs a combination of random forest analysis and a human health risk model to investigate the effects of variables on Cd levels in rice grains,with the goal of quantifying their contributions and elucidating their relationships.The results indicated that the 15 selected variables collectively explained 47.36%of the variation in Cd content,with the top three variables being soil pH,distance from industrial park,and soil Zn.The majority of variables exhibited threshold effects on Cd levels in rice grains.By visualizing the interaction between Soil pH,distance from industrial park,and soil Zn with Cd levels in rice,we demonstrate the threshold effects of them on Cd level in rice grains,thereby providing further insight into the variation observed.Furthermore,oral intake of rice has been identified as the primary route of human exposure,significantly contributing to overall exposure pathways.Understanding these interactions is crucial for gaining insights into the underlying processes driving Cd pollution and fostering sustainable development within the industry.Our findings underscore the crucial need to consider multiple environmental variables and their interactions when managing heavy metals(HMs)contamination and mitigating health risks.展开更多
Leaf angle is a pivotal agronomic trait that significantly influences crop architecture and yield.Plant hormones,such as auxin,play a critical role in regulating leaf angle;however,the underlying molecular mechanisms ...Leaf angle is a pivotal agronomic trait that significantly influences crop architecture and yield.Plant hormones,such as auxin,play a critical role in regulating leaf angle;however,the underlying molecular mechanisms remain to be fully elucidated.Here,we reveal that the auxin response factor gene,OsARF12,which is highly expressed in the leaf lamina joint,negatively regulates rice(Oryza sativa L.)leaf angle via affecting shoot gravitropism.Overexpression lines of OsARF12 exhibit more erect leaf angles,while the osarf12 mutants display enlarged leaf angles compared to the wild type.Further studies demonstrate that OsARF12 directly activates the expression of Loose Plant Architecture1(LPA1)and LAZY1 by binding to their promoters.The osarf12 mutant presents impaired shoot gravitropism,a phenotype consistent with that of the lpa1 and lazy1 mutants.Collectively,we elucidate the biological functions of OsARF12,which modulates leaf angle through its impact on shoot gravitropism by regulating the expression levels of LPA1 and LAZY1.This study provides insight into the role of auxin in determining rice leaf angle,potentially holding profound effects for the optimization of crop architecture.展开更多
Flooding stress is a major adverse condition during the emergence period of direct-seeded rice.This study investigated the use of wood vinegar as a seed soaking treatment to enhance rice seedling rates under flooding ...Flooding stress is a major adverse condition during the emergence period of direct-seeded rice.This study investigated the use of wood vinegar as a seed soaking treatment to enhance rice seedling rates under flooding stress,exploring both the methodology and physiological mechanisms involved.The optimal seed soaking concentration was determined through a gradient experiment,followed by a multi-cultivar validation test.The physiological mechanism of wood vinegar soaking on seedling emergence was analyzed by measuring the electrical conductivity of the flooding water,the changes in starch and soluble sugar contents in the grains and sprouts,and the dynamics ofα-amylase activity and antioxidant-related enzyme activities in the sprouts.The results showed that soaking rice seeds in a wood vinegar solution at a low concentration significantly enhanced the emergence of rice seedlings under flooding conditions,with a 100-fold dilution having the most pronounced effect,increasing seedling rates by 50.6%-60.0%.Further analysis indicated that wood vinegar treatment enhanced seedling establishment by inducing a significant increase inα-amylase activity,leading to a 74.9%-213.6%increase in soluble sugar content in the sprouts during 2-8 d after flooding stress compared with the control.Additionally,the treatment increased superoxide dismutase and peroxidase activities in the sprouts,mitigating lipid peroxidation of the cell membranes,and notably lower water electrical conductivity was observed in wood vinegar-treated seeds compared with the control.In conclusion,soaking rice seeds in a 100-fold diluted wood vinegar solution improves rice seedling rates under flooding stress by mitigating oxidative damage and maintaining energy supply.This approach is valuable for developing cost-effective seed treatment technologies and offering novel strategies to improve seedling rates and uniformity of direct-seeded rice under flooding conditions.展开更多
Rice production is increasingly challenged by flooding stress because of global warming and rising sea levels.As the world’s most important staple crop,rice is highly vulnerable to anaerobic and submergence condition...Rice production is increasingly challenged by flooding stress because of global warming and rising sea levels.As the world’s most important staple crop,rice is highly vulnerable to anaerobic and submergence conditions that occur during flooding,particularly at the germination and vegetative stages.Anaerobic environments hinder seedling establishment during germination,while prolonged submergence during the vegetative stage impairs growth,ultimately reducing yield and grain quality.These stresses,driven by extended inundation,trigger a cascade of detrimental physiological responses and represent a major barrier to stable rice production and global food security.In this review,we examine the effects of flooding on rice growth at both the germination and vegetative stages.We further summarize recent advances in the identification of flooding-tolerant germplasm,QTL mapping,genome-wide association study,transcriptomic and proteomic analyses,and other molecular studies.Subsequently,we highlight potential cultivation and regulatory strategies,including genetic,morphological,physiological,and endogenous hormone-related approaches,aimed at enhancing tolerance to anaerobic and submergence stress.Together,these approaches underscore the promise of integrating molecular insights with agronomic practices to mitigate flooding damage and support sustainable rice production.展开更多
Plant viruses pose significant threats to agriculture,with many vectored by insect pests.The entry of viruses and their encoded proteins into the host nucleus is a critical step for promoting some viral replication an...Plant viruses pose significant threats to agriculture,with many vectored by insect pests.The entry of viruses and their encoded proteins into the host nucleus is a critical step for promoting some viral replication and enabling systemic infection.Laodelphax striatellus,also known as the small brown planthopper(SBPH),is an efficient vector for rice stripe virus(RSV),one of the most damaging viruses of rice.In this study,we demonstrate that RSV infection induces the expression of genes in both the classical and non-classical nuclear import pathways of SBPH.A gene belonging to the importinβfamily,importin 5(LsIPO5),was upregulated by 84%in SBPH midguts infected with RSV.The nuclear localization signal(NLS,^(168)YRSPSKKRHKYV^(179))is located within the nonstructural protein NS3 directly bound to LsIPO5,thereby facilitating NS3nuclear entry.Moreover,a RING-type E3 ligase(LsRING)in SBPH,which mediated the ubiquitination of NS3 in the insect vector,enhanced NS3 binding to LsIPO5 and facilitated NS3 perinuclear localization.Combined treatment of SBPH with both ds IPO5 and ds RING significantly reduced RSV loads,highlighting the importance of LsIPO5 and NS3 ubiquitination cooperation in facilitating viral replication.Our findings provide new insights into synergistic molecular mechanisms that govern RSV infection and suggest potential therapeutic targets to control viral transmission through their insect vectors.展开更多
In China,farmers have increasingly adopted direct-seeded rice(DSR).While various impacts of DSR have been studied,limited evidence exists regarding the effect of DSR adoption on pesticide use.This study examines the i...In China,farmers have increasingly adopted direct-seeded rice(DSR).While various impacts of DSR have been studied,limited evidence exists regarding the effect of DSR adoption on pesticide use.This study examines the impact of DSR adoption on pesticide use utilizing data from a 2018 survey of 982 rice farmers in China's Yangtze River Basin.The endogenous treatment-regression and switching regression models are employed to address self-selection bias.The results indicate that,after accounting for self-selection,DSR adopters spend 401.72 CNY ha^(-1) more on pesticides compared to non-adopters.Although DSR adoption significantly increases the use of insecticides,fungicides and herbicides,its impact is most pronounced for insecticide expenditure and least pronounced for herbicide expenditure.The findings remain robust when altering the dependent variable,truncating the research sample,and modifying the estimation method.Heterogeneous analysis reveals that DSR adoption has a stronger positive impact on pesticide expenditure among farmers below 60 years of age,with at least 6 years of education,and managing rice sown areas less than 2 ha.Based on these findings,this study recommends enhancing complementary techniques for DSR,improving the dissemination of DSR cultivation technologies,and strengthening socialized services.This research provides a comprehensive assessment of DSR's advantages and disadvantages,particularly regarding pesticide use,offering important policy implications for pesticide reduction.展开更多
Investigating the biological processes of iron(Fe)homeostasis is crucial for comprehending crop genetic improvement,which in turn helps address human malnutrition.This study utilized phenotyping,ionomics,and transcrip...Investigating the biological processes of iron(Fe)homeostasis is crucial for comprehending crop genetic improvement,which in turn helps address human malnutrition.This study utilized phenotyping,ionomics,and transcriptome analysis to uncover the regulatory mechanism of Fe homeostasis in rice under different Fe concentrations and during Fe supplementation.Our results showed both Fe deficiency and excess impede rice growth,with Fe excess exerting a more severe impact,particularly on the roots.The decrease in crown roots under excessive Fe conditions likely serves as an adaptive mechanism to counteract Fe toxicity.Transcriptomic analysis identified 4652 differentially expressed genes affected by Fe stress and supplementation.When Fe is supplemented to Fe-deficient rice,there are upregulations in the expression of genes related to Fe ion concentration and Fe homeostasis at 10 min and 2 h after supplementation,respectively,along with a brief downregulation at 30 min.This indicated a protective mechanism in the roots during Fe uptake.Notably,shoots with a lack of Fe accumulation did not show re-entry of Fe after supplementation,and there was a sustained downregulation of Fe-regulated genes.This suggests that the signaling from roots to shoots influences the response of shoots to Fe supplementation in rice.The molecular changes in Fe homeostasis discovered in this study can contribute to the improvement of rice.展开更多
Atopic dermatitis(AD),which is associated with inflammatory response and skin damage,is increasing worldwide in recent years.Interest is increasing in the protective effects of natural and fermented products on the pr...Atopic dermatitis(AD),which is associated with inflammatory response and skin damage,is increasing worldwide in recent years.Interest is increasing in the protective effects of natural and fermented products on the prevention and treatment of AD.This study aimed to investigate the ameliorative effects of a new rice milk product fermented with Saccharomyces cerevisiae on AD mice.Fermented rice milk could effectively attenuated the symptoms of AD mice,such as skin lesions,scaliness and lichenification.Furthermore,fermented rice milk decreased the levels of inflammatory cytokines interleukin(IL)-4,IL-6 and tumor necrosis factor(TNF)-αin 2,4-dinitrochlorobenzene(DNCB)induced mice.It was also observed that fermented rice milk treatment downregulated the levels of p-IKBα,p-NF-κB,p-ERK,p-JNK and p-p38 proteins expression in skin of AD mice,which showed the anti-inflammatory function of fermented rice milk,mainly via the NF-κB and MAPK signaling pathways.In addition,S.cerevisiae fermentation reduced original flavor of rice milk,and increased the acid and alcohol compounds.Overall,the findings indicated that S.cerevisiae fermentation improved the flavor characteristics of rice milk,and fermented rice milk exhibited protective effects against DNCB-induced atopic dermatitis by downregulating NF-κB and MAPK signaling pathways and protecting the skin barrier.展开更多
Canopy photosynthesis,rather than leaf photosynthesis,is highly related to plant biomass and yield formation.Studying canopy photosynthesis and identifying the parameters that control it can help optimize agricultural...Canopy photosynthesis,rather than leaf photosynthesis,is highly related to plant biomass and yield formation.Studying canopy photosynthesis and identifying the parameters that control it can help optimize agricultural management and achieve crop yield potential.Compared with traditional parameters,canopy occupation volume(COV)offers an integrative parameter on canopy architecture related to canopy photosynthetic rates.In this study,we developed a high-throughput method to derive COV for different rice varieties.We first used multi-perspective two-dimensional imaging to reconstruct three-dimensional point clouds of rice plants and developed a suite of pipelines to calculate plant height,leaf number,tiller number,and biomass,with R^(2) values of 91.8%,95.9%,82.3%,and 94.3%,respectively.We further employed point cloud data to reconstruct the surfaces of rice plants and construct a virtual canopy model of the rice population.Light distribution was simulated using a ray-tracing algorithm and canopy photosynthetic rates were simulated via photosynthetic rate-incident light intensity curve fitting.Furthermore,we systematically explored the relationships between canopy phenotypes and photosynthetic rates,and found that COV was the most effective predictor of canopy photosynthesis,achieving an R^(2) value of 92.1%.Adjustment in atmospheric transmittance showed that COV strongly correlated with canopy photosynthesis under different light conditions,with higher accuracy observed under diffuse light.Variations in planting density confirmed that this correlation remained strong at the community level.In summary,this study demonstrates that COV is closely linked to simulated canopy photosynthesis and the developed pipeline can support future agronomic and breeding research.展开更多
As a vital food crop,rice is an important part of global food crops.Studying the spatiotemporal changes in rice cultivation facilitates early prediction of production risks and provides support for agricultural policy...As a vital food crop,rice is an important part of global food crops.Studying the spatiotemporal changes in rice cultivation facilitates early prediction of production risks and provides support for agricultural policy decisions related to rice.With the increasing application of satellite remote sensing technology in crop monitoring,remote sensing for rice cultivation has emerged as a novel approach,offering new perspectives for monitoring rice planting.This paper briefly outlined the current research and development status of satellite remote sensing for monitoring rice cultivation both at home and abroad.Foreign scholars have made innovations in data sources and methodologies for satellite remote sensing monitoring,and utilized multi-source satellite information and machine learning algorithms to enhance the accuracy of rice planting monitoring.Scholars in China have achieved significant results in the study of satellite remote sensing for monitoring rice cultivation.Their research and application in monitoring rice planting areas provide valuable references for agricultural production management.However,satellite remote sensing monitoring of rice still faces challenges such as low spatiotemporal resolution and difficulties related to cloud cover and data fusion,which require further in-depth investigation.Additionally,there are shortcomings in the accuracy of remote sensing monitoring for fragmented farmland plots and smallholder farming.To address these issues,future efforts should focus on developing multi-source heterogeneous data fusion analysis technologies and researching monitoring systems.These advancements are expected to enable high-precision large-scale acquisition of rice planting information,laying a foundation for future smart agriculture.展开更多
As sessile organisms,plants must adapt various stresses.Accordingly,they have evolved several plant-specific growth and developmental processes.WRKY53 is a member of the WRKY transcription factor family,which plays a ...As sessile organisms,plants must adapt various stresses.Accordingly,they have evolved several plant-specific growth and developmental processes.WRKY53 is a member of the WRKY transcription factor family,which plays a crucial role in rice growth and development,stress response,and hormone signal transduction.This review discusses the role of WRKY53 in stress response,focusing on its functions in cold tolerance,salt tolerance,disease resistance,and pest defense,and explores its role in regulating rice leaf senescence and seed germination.This article also proposes future research directions,including functional genomics studies,protein interaction network analyses,hormone signal transduction pathways,genetic improvement strategies,applications of gene editing technologies,molecular basis of stress responses,cross-species functional conservation,and bioinformatics and comparative genomics research.This review highlights the importance of WRKY53 in rice biology and provides new perspectives and strategies for future research and genetic improvement of rice.展开更多
基金funded by the National Key Research and Development Program of China(2016yfd30300508,2017YFD0301602,and 2018yfd0301105)the Science&Technology Development Fund of Fujian Agriculture and Forestry University,China(kf2015043)。
文摘The rice ratooning system has attracted increasing attention in southern China due to its low carbon emissions and high yield potential.However,the net carbon budget and underlying mechanisms remain unclear.Three rice cropping systems were established in this trial experiment conducted from 2021 to 2022 in Fuzhou(25°05'N,119°13'E),Southeast China:ratooning rice(RR:MC+RSR)pattern for rice ratooning,single-cropping rice(LR_(1)),and double-cropping rice(DC:ER+LR_(2)).The closed static dark box gas collection,dry matter determination,life cycle assessment(LCA)etc.approaches were utilized to investigate the mechanism of“high carbon fixation–low emissions”mechanism in RR.A comprehensive assessment was conducted across multiple dimensions,including crop yield,greenhouse gas(GHG)emissions,carbon and nitrogen footprints,resource use efficiency,carbon sequestration capacity,and carbon budget balance.Results showed that the average daily yield of ratoon season rice(RSR)across RR treatments from 2021 to 2022 was 28.21–47.40%higher than that of the main crop(MC)and LR_(1),and the average daily yield of RR was 13.50–27.76%higher than DC.This yield advantage was attributed to a 32.32–39.26%increase in the allocation of^(13)C-labeled photosynthetic products(including non-structural carbohydrates,NSCs)to panicle organs,and a 21.77–43.51%reduction in allocation to underground roots and soil.Furthermore,the average daily global warming potential(GWP)was 16.44 kg CO_(2)-eq ha^(–1)for RR,24.99 kg CO_(2)-eq ha^(–1)for LR_(1),and 21.32 kg CO_(2)-eq ha^(–1)for DC.Specifically,the average daily GWP of ratoon rice was 34.21%lower than that of LR_(1) and 22.90%lower than double-cropping rice.Similarly,the average daily greenhouse gas intensity(GHGI)of ratoon rice was 62.28%lower than LR_(1) and 28.96%lower than double-cropping rice.In terms of carbon and nitrogen footprints,the ratoon rice system exhibited average daily values of 34.54 kg CO_(2)-eq ha^(–1)and 0.47 kg N ha^(–1),respectively.In comparison,LR_(1) had values of 45.63 kg CO_(2)-eq ha^(–1)and 0.49 kg N ha^(–1),while double-cropping rice showed 43.38 kg CO_(2)-eq ha^(–1)and 0.53 kg N ha^(–1).These values represent reductions of 24.30%in carbon footprint and4.28%in nitrogen footprint relative to LR_(1),and 20.38 and 11.45%relative to double-cropping rice,respectively.Moreover,the average annual carbon budget surplus across systems was 22,380.01 kg CO_(2)-eq ha^(–1)for ratoon rice(MC+RSR),11,228.54 kg CO_(2)-eq ha^(–1)for LR_(1),and 23,772.15 kg CO_(2)-eq ha^(–1)for DC.Consequently,the resource utilization efficiency of the RR was 24.42 and 47.50%higher than that of single-cropping and double-cropping systems,respectively.Average daily economic returns also increased by 32.71 and 80.75%,respectively.These findings provide a robust theoretical foundation and practical guidance for advancing agricultural carbon neutrality technologies and ensuring food security.
基金funded by the National Natural Science Foundation of China (32172108 and 32301940)the Young Elite Scientists Sponsorship Program by the China Association for Science and Technology (2023QNRC001)+2 种基金the China Postdoctoral Science Foundation (2022M710489)the Chinese Scholarship Council (202310930003)the National Key Research and Development Program of China (2022YFD2301004)。
文摘The breakthrough in super hybrid rice yield has significantly contributed to China’s and global food security.However,the inherent conflict between high productivity and environmentally sustainable agriculture poses substantial challenges.Issues such as water scarcity,energy crises,escalating greenhouse gas emissions,and diminishing farm profitability threaten longterm agricultural sustainability.In response,we applied a holistic food–carbon–nitrogen–water–energy–profit (FCNWEP)nexus framework to comprehensively assess the sustainability of distinct crop management strategies across three subsites in Central China.Field experiments were conducted in Hubei and Hunan provinces from 2017 to 2021 using a widely adopted elite super hybrid rice cultivar (Y-liangyou 900).Four crop management treatments were implemented:a control(CK,0 kg N ha^(-1)),conventional crop management (CCM,210–250 kg N ha^(-1),7:3 basal:mid-tiller fertilizer ratio),and two integrated crop management (ICM) treatments (ICM1,180–210 kg N ha^(-1),5:2:3 basal:mid-tiller:panicle initiation fertilizer ratio;ICM2,240–270 kg N ha^(-1),5:2:2:1 basal:mid-tiller:panicle initiation:flowering fertilizer ratio).Variables assessed included grain yield,carbon footprint,nitrogen footprint,water footprint,energy footprint,nitrogen use efficiency,and economic benefits.Our results showed significant yield variations,with ICM2 consistently outperforming CCM and ICM1across all three sites.In Jingzhou,Suizhou,and Changsha,ICM2’s grain yield was 30.2,24.7,and 13.3%higher than CCM,respectively.Net profits under ICM2 exceeded those of CCM and ICM1 by 31.8 and 115.2%in Jingzhou,32.2 and 109.9%in Suizhou,and 15.4 and 34.0%in Changsha,respectively.Integrated crop management,particularly ICM2,demonstrated improved nitrogen and energy use efficiency,leading to reduced carbon,nitrogen,water,and energy footprints.Overall,composite sustainability scores derived from the FCNWEP framework indicated that both ICM2 and ICM1 exhibited higher sustainability levels compared to CCM.This study provides valuable insights into practical management methodologies and offers recommendations for enhancing agricultural sustainability.
基金support of the Japanese Government(Monbukagakusho)Scholarship for his studies in Japansupported by the Yamagata University YU-COE(S)program and by the Advanced Agri-food System Research Center of Yamagata University,Japan+2 种基金financially supported by a Japan Society for the Promotion of Science(JSPS)Grant-in-Aid for Scientific Research(26310304)Yamagata University YU-COE(S)programby the Advanced Agri-food System Research Center of Yamagata University,Japan。
文摘To examine the impact of anthropogenic land reconstruction,particularly the consolidation of small terraces into larger fields,on soil organic carbon(SOC),total nitrogen(TN)dynamics,rice yield,and its components,soil and plant samples were collected from seven newly reconstructed fields in Japanese Andosols in Tochigi,Japan.Samples were obtained from both the former low-and high-elevation sides within each field plot.During harvest season,nine rice plants were randomly selected from each plot(0.675 m^(2),comprising 3 rows and 3 hills per row),collected from a 3-m stretch along both the east(former low side)and west(former high side)ridges.Soil cores were collected from identical plots at two depths(0–15 and 15–30 cm)and combined into one composite sample per layer.Rice plant samples were air-dried for two weeks until reaching constant moisture content,after which stems and ears were separated and weighed to determine biomass,yield,yield components,and nitrogen uptake.This indicated that land reconstruction significantly affected rice yield and its components between the two sides of all field plots.SOC,TN,and their decomposition following land reconstruction showed notable changes,especially in the 15–30 cm subsurface soil layer.Additionally,grain weight demonstrated significant correlation with SOC,TN,and carbon decomposition in both the 0–15 and 15–30 cm layers,indicating that soil fertility to a depth of 30 cm was crucial for rice productivity after land reconstruction.
基金supported by the Natural Science Foundation of Zhejiang Province,China(Grant No.LTGN24C020004)the Enterprise Cooperation Project,China(Grant No.HRJYH-202330)+1 种基金the Zhejiang Province Pioneer and Bellwethers Research&Development Project of Science and Technology,China(Grant No.2023C02014)the Central Public-Interest Scientific Institution Basal Research Fund,China(Grant No.CPSIBRFCNRRI-202303).
文摘To elucidate the variations in volatile organic compounds(VOCs)among widely cultivated japonica rice varieties in China and offer novel perspectives on flavor formation during rice-based beer brewing,nine prevalent Chinese japonica rice variaties were selected as experimental materials.Comprehensive analyses were conducted to investigate three key aspects:differences in VOCs among the selected japonica rice varieties,the retention of rice VOCs after beer brewing,and the influence of rice additives on beer sensory characteristics.Results showed that the total contents of VOCs in Wuyoudao 4(WYD4)and Nangeng 9108(NG9108)were significantly higher than those in the other varieties(P<0.05).The popcorn-flavor compound 2-acetyl-1-pyrroline(2-AP)was detected in Suigeng 27(SG27,26.80 ng/g),WYD4(25.25 ng/g),NG9108(21.18 ng/g),and Suigeng 18(SG18,11.62 ng/g),with the levels in the first three varieties significantly higher than those in SG18.Circular heatmap analysis classified the nine japonica varieties into three major categories:fragrant japonica from North China(WYD4),fragrant japonica from South China(NG9108),and others.Ten characteristic VOCs across the japonica varieties were identified by headspace solid-phase microextraction gas chromatography-mass spectrometry(HS-SPME-GC-MS).Using beer brewed with whole wheat as the control,we analyzed the VOCs and sensory characteristics of beer brewed with fragrant japonica rice as an additive.We found that rice VOCs were not detected in beer brewed with fragrant japonica rice as an additive.Adding rice promoted the formation of banana-like flavors and reduced the beer’s richness and mellowness.
基金supported by the National Key Research and Development Program of China(2020YFA0907600)the Biological Breeding-National Science and Technology Major Project(2024ZD04080)+1 种基金the National Natural Science Foundation of China(32270252)the Natural Science Foundation of Guangdong Province(2024A1515011085).
文摘Photorespiration consumes photosynthetically fixed carbon and reduces yields by 20%–50%in C3 crops.In an attempt to increase photosynthetic efficiency in rice by bypassing the carbon-consuming process of photorespiration,a photorespiratory bypass consisting of Chlamydomonas reinhardtii glycolate dehydrogenase and Cucurbita maxima malate synthase(termed the GMS bypass)was introduced into the rice cultivar Zhonghua 11 and osplgg1b,a mutant of the rice chloroplast glycolate transporter,to generate GMS/ZH11 and GMS/osplgg1b transgenic plants.The GMS bypass reduced photorespiration and increased photosynthesis in the transgenic plants.The straw biomass of GMS/ZH11 and GMS/osplgg1b increased by up to 16.0%and 85.7%,respectively.The yield of GMS/ZH11 increased by 22.0%–34.7%in paddy fields.Thus,the GMS bypass can increase photosynthetic efficiency and yield in rice.
基金funded by the National Key R&D Project‘Innovation and Integration of Key Technologies for Integration of Agricultural Machinery and Agronomy in Weak Links of Hybrid Mid-season Rice in Hilly Areas of Southwest China’(2023YFD2301901).
文摘Chilo suppressalis(Walker)is one of the most important rice pests worldwide,posing a significant challenge to effective control.To develop a precision-timed,eco-friendly management strategy,overwintering population investigation and dynamic monitoring of C.suppressalis populations were conducted in the Meishan region of Sichuan,China,from 2023 to 2024.The optimal timing for insecticide application was estimated,followed by field trials evaluating the efficacy of different insecticides.Results demonstrated that the peak emergence of first-generation adults typically occurred in early July(under the environmental conditions of the Meishan region),with the ambient humidity below 75%and temperature around 29◦C.Pesticide efficacy trials show that insecticide combinations exhibited superior control.Notably,a combined treatment of emamectin benzoate⋅methoxyfenozide+chlorantraniliprole achieved the highest control efficacy(90.05%)and a corresponding yield of 12,491.55 kg/ha.All tested treatments were determined to be safe for rice growth.Furthermore,this optimized strategy resulted in notable economic benefits,including a 50%reduction in pesticide usage and cost savings of 4796.15 CNY compared to conventional practices.This study provides valuable insights into sustainable rice production and pest management and,for the first time,proposes a precision application time window based on intelligent monitoring.
基金supported by the National Natural Science Foundation of China(32301845)GuangDong Basic and Applied Basic Research Foundation(2022A1515012339)+3 种基金the National Key R&D Program of China(2024YFD1200801)Seed industry revitalization project of special fund for rural revitalization strategy in Guangdong Province(2024-NPY-00-001)Modern Seed Industry Innovation Capacity Enhancement Progject of Guangdong Academy of Agricultural Sciences,Elite Rice Plan of GDRRI(2023YG01)Guangdong Key Laboratory of Rice Science and Technology(2023B1212060042).
文摘Leaf thickness in rice critically influences photosynthetic efficiency and yield,yet its genetic basis remains poorly understood,with few functional genes previously characterized.In this study,we employ a pangenome-wide association study(Pan-GWAS)on 302 diverse rice accessions from southern China,identifying 49 quantitative trait loci(QTLs)associated with leaf thickness.The most significant locus,qLT9,is fine-mapped to a 79-kb region on chromosome 9.Transcriptomic and genomic sequence analyses identify LOC_Os09g33480,which encodes a protein belonging to Multiple Organellar RNA Editing Factor family,as the key candidate gene.Overexpression and complementation transgenic experiments confirm LOC_Os09g33480(OsLT9)as the functional gene underlying qLT9,demonstrating a 24-bp Indel in its promoter correlates with the expression levels and leaf thickness.Notably,OsLT9 overexpression lines show not only thicker leaf,but also significantly enhanced photosynthetic efficiency and grain yield,establishing a link between leaf thickness modulation and yield enhancement.Population genomic analyses indicate strong selection for OsLT9 during domestication and breeding,with modern cultivars favoring thick leaf haplotype of OsLT9.This study establishes OsLT9 as a key regulator controlling leaf thickness in rice,and provides a valuable genetic resource for molecular breeding of high-yielding rice through optimization of plant architecture.
基金supported by the Project of Zhongshan Biological Breeding Laboratory,China(Grant Nos.ZSBBL-KY2024-01 and ZSBBL-KY2025-2)the Key R&D Program of Jiangsu Province,China(Grant No.BE2022335)the Fund of Priority Academic Program Development of Jiangsu Higher Education Institutions,China.
文摘Accumulating evidence from recent studies has highlighted the critical regulatory functions of non-histone protein acetylation in rice biological processes.This review systematically synthesizes current advances in characterizing the functional attributes and regulatory mechanisms of non-histone acetylation in rice,with a specific focus on its roles in regulating gene expression,modulating metabolic enzyme activities,and mediating stress responses.Emerging studies demonstrate that non-histone acetylation dynamically modulates transcription factors,metabolic enzymes,and other pivotal functional proteins to orchestrate essential physiological processes,including growth and development,photosynthetic efficiency,and environmental stress adaptation.Using mass spectrometry,gene editing,and related technologies,researchers have identified multiple acetyltransferases and deacetylases that regulate protein stability,subcellular localization,and protein-protein interactions.Despite these advances,challenges persist,such as the complexity of the acetylation regulatory networks and species-specific differences among cereal crops.Future investigations should integrate multi-omics approaches to elucidate the molecular mechanisms of this post-translational modification,thereby facilitating the development of targeted genetic engineering strategies for rice improvement.
基金supported by the GDAS’Project of Science and Technology Development(No.2022GDASZH-2022010104-2)Guangdong Major Project of Basic and Applied Basic Research(No.2023B0303000006).
文摘Understanding Cd contamination in the soil-rice ecosystem and the underlying its threshold and interaction effects is crucial for controlling Cd pollution and ensuring food safety.Although the quantitative relationships between Cd and environmental variables have been extensively studied,the threshold and interaction effects of multi-source environmental variables remain largely unexplored.This study employs a combination of random forest analysis and a human health risk model to investigate the effects of variables on Cd levels in rice grains,with the goal of quantifying their contributions and elucidating their relationships.The results indicated that the 15 selected variables collectively explained 47.36%of the variation in Cd content,with the top three variables being soil pH,distance from industrial park,and soil Zn.The majority of variables exhibited threshold effects on Cd levels in rice grains.By visualizing the interaction between Soil pH,distance from industrial park,and soil Zn with Cd levels in rice,we demonstrate the threshold effects of them on Cd level in rice grains,thereby providing further insight into the variation observed.Furthermore,oral intake of rice has been identified as the primary route of human exposure,significantly contributing to overall exposure pathways.Understanding these interactions is crucial for gaining insights into the underlying processes driving Cd pollution and fostering sustainable development within the industry.Our findings underscore the crucial need to consider multiple environmental variables and their interactions when managing heavy metals(HMs)contamination and mitigating health risks.
基金funded by the National Natural Science Foundation of China(31871592)the Fundamental Research Funds for the Central Universities(2042022kf0015)+1 种基金the Creative Research Groups of the Natural Science Foundation of Hubei Province(2020CFA009)the Project for Technology Innovation of Hubei Province(2024BBA005).
文摘Leaf angle is a pivotal agronomic trait that significantly influences crop architecture and yield.Plant hormones,such as auxin,play a critical role in regulating leaf angle;however,the underlying molecular mechanisms remain to be fully elucidated.Here,we reveal that the auxin response factor gene,OsARF12,which is highly expressed in the leaf lamina joint,negatively regulates rice(Oryza sativa L.)leaf angle via affecting shoot gravitropism.Overexpression lines of OsARF12 exhibit more erect leaf angles,while the osarf12 mutants display enlarged leaf angles compared to the wild type.Further studies demonstrate that OsARF12 directly activates the expression of Loose Plant Architecture1(LPA1)and LAZY1 by binding to their promoters.The osarf12 mutant presents impaired shoot gravitropism,a phenotype consistent with that of the lpa1 and lazy1 mutants.Collectively,we elucidate the biological functions of OsARF12,which modulates leaf angle through its impact on shoot gravitropism by regulating the expression levels of LPA1 and LAZY1.This study provides insight into the role of auxin in determining rice leaf angle,potentially holding profound effects for the optimization of crop architecture.
基金supported by the National Key Research and Development Program of China(Grant No.2023YFD2301300)the National Rice Industry Technology System,China(Grant No.CARS-01).
文摘Flooding stress is a major adverse condition during the emergence period of direct-seeded rice.This study investigated the use of wood vinegar as a seed soaking treatment to enhance rice seedling rates under flooding stress,exploring both the methodology and physiological mechanisms involved.The optimal seed soaking concentration was determined through a gradient experiment,followed by a multi-cultivar validation test.The physiological mechanism of wood vinegar soaking on seedling emergence was analyzed by measuring the electrical conductivity of the flooding water,the changes in starch and soluble sugar contents in the grains and sprouts,and the dynamics ofα-amylase activity and antioxidant-related enzyme activities in the sprouts.The results showed that soaking rice seeds in a wood vinegar solution at a low concentration significantly enhanced the emergence of rice seedlings under flooding conditions,with a 100-fold dilution having the most pronounced effect,increasing seedling rates by 50.6%-60.0%.Further analysis indicated that wood vinegar treatment enhanced seedling establishment by inducing a significant increase inα-amylase activity,leading to a 74.9%-213.6%increase in soluble sugar content in the sprouts during 2-8 d after flooding stress compared with the control.Additionally,the treatment increased superoxide dismutase and peroxidase activities in the sprouts,mitigating lipid peroxidation of the cell membranes,and notably lower water electrical conductivity was observed in wood vinegar-treated seeds compared with the control.In conclusion,soaking rice seeds in a 100-fold diluted wood vinegar solution improves rice seedling rates under flooding stress by mitigating oxidative damage and maintaining energy supply.This approach is valuable for developing cost-effective seed treatment technologies and offering novel strategies to improve seedling rates and uniformity of direct-seeded rice under flooding conditions.
基金supported by the National Natural Science Foundation of China(Grant Nos.32160501 and 32201901)the Accelerated Breeding Initiative of the Consultative Group on International Agricultural Research(Grant No.INIT-01)+2 种基金the Natural Science Foundation of Guangxi,China(Grant No.2021GXNSFAA220026)the Program on National Modern Agricultural Technology System Guangxi Innovation Team,China(Grant No.nycytxgxcxtd-2021-01-04)the Advantage Team Project of Guangxi Academy of Agricultural Sciences,China(Grant No.2026YT070).
文摘Rice production is increasingly challenged by flooding stress because of global warming and rising sea levels.As the world’s most important staple crop,rice is highly vulnerable to anaerobic and submergence conditions that occur during flooding,particularly at the germination and vegetative stages.Anaerobic environments hinder seedling establishment during germination,while prolonged submergence during the vegetative stage impairs growth,ultimately reducing yield and grain quality.These stresses,driven by extended inundation,trigger a cascade of detrimental physiological responses and represent a major barrier to stable rice production and global food security.In this review,we examine the effects of flooding on rice growth at both the germination and vegetative stages.We further summarize recent advances in the identification of flooding-tolerant germplasm,QTL mapping,genome-wide association study,transcriptomic and proteomic analyses,and other molecular studies.Subsequently,we highlight potential cultivation and regulatory strategies,including genetic,morphological,physiological,and endogenous hormone-related approaches,aimed at enhancing tolerance to anaerobic and submergence stress.Together,these approaches underscore the promise of integrating molecular insights with agronomic practices to mitigate flooding damage and support sustainable rice production.
基金supported by the Natural Science Foundation of Jiangsu Province,China(BK20240902 and BK20240904)the National Natural Science Foundation of China(32272533)。
文摘Plant viruses pose significant threats to agriculture,with many vectored by insect pests.The entry of viruses and their encoded proteins into the host nucleus is a critical step for promoting some viral replication and enabling systemic infection.Laodelphax striatellus,also known as the small brown planthopper(SBPH),is an efficient vector for rice stripe virus(RSV),one of the most damaging viruses of rice.In this study,we demonstrate that RSV infection induces the expression of genes in both the classical and non-classical nuclear import pathways of SBPH.A gene belonging to the importinβfamily,importin 5(LsIPO5),was upregulated by 84%in SBPH midguts infected with RSV.The nuclear localization signal(NLS,^(168)YRSPSKKRHKYV^(179))is located within the nonstructural protein NS3 directly bound to LsIPO5,thereby facilitating NS3nuclear entry.Moreover,a RING-type E3 ligase(LsRING)in SBPH,which mediated the ubiquitination of NS3 in the insect vector,enhanced NS3 binding to LsIPO5 and facilitated NS3 perinuclear localization.Combined treatment of SBPH with both ds IPO5 and ds RING significantly reduced RSV loads,highlighting the importance of LsIPO5 and NS3 ubiquitination cooperation in facilitating viral replication.Our findings provide new insights into synergistic molecular mechanisms that govern RSV infection and suggest potential therapeutic targets to control viral transmission through their insect vectors.
基金supported by the General Project of Humanities and Social Sciences Research of the Ministry of Education of China(24YJA790085)the Science and Technology Innovation Program of Beijing Institute of Technology,China(2024CX01020)。
文摘In China,farmers have increasingly adopted direct-seeded rice(DSR).While various impacts of DSR have been studied,limited evidence exists regarding the effect of DSR adoption on pesticide use.This study examines the impact of DSR adoption on pesticide use utilizing data from a 2018 survey of 982 rice farmers in China's Yangtze River Basin.The endogenous treatment-regression and switching regression models are employed to address self-selection bias.The results indicate that,after accounting for self-selection,DSR adopters spend 401.72 CNY ha^(-1) more on pesticides compared to non-adopters.Although DSR adoption significantly increases the use of insecticides,fungicides and herbicides,its impact is most pronounced for insecticide expenditure and least pronounced for herbicide expenditure.The findings remain robust when altering the dependent variable,truncating the research sample,and modifying the estimation method.Heterogeneous analysis reveals that DSR adoption has a stronger positive impact on pesticide expenditure among farmers below 60 years of age,with at least 6 years of education,and managing rice sown areas less than 2 ha.Based on these findings,this study recommends enhancing complementary techniques for DSR,improving the dissemination of DSR cultivation technologies,and strengthening socialized services.This research provides a comprehensive assessment of DSR's advantages and disadvantages,particularly regarding pesticide use,offering important policy implications for pesticide reduction.
基金supported by the National Key Research and Development Program,China(Grant No.2023YFD2301900)the Natural Science Foundation of Sichuan Province,China(Grant No.23NSFSC0055).
文摘Investigating the biological processes of iron(Fe)homeostasis is crucial for comprehending crop genetic improvement,which in turn helps address human malnutrition.This study utilized phenotyping,ionomics,and transcriptome analysis to uncover the regulatory mechanism of Fe homeostasis in rice under different Fe concentrations and during Fe supplementation.Our results showed both Fe deficiency and excess impede rice growth,with Fe excess exerting a more severe impact,particularly on the roots.The decrease in crown roots under excessive Fe conditions likely serves as an adaptive mechanism to counteract Fe toxicity.Transcriptomic analysis identified 4652 differentially expressed genes affected by Fe stress and supplementation.When Fe is supplemented to Fe-deficient rice,there are upregulations in the expression of genes related to Fe ion concentration and Fe homeostasis at 10 min and 2 h after supplementation,respectively,along with a brief downregulation at 30 min.This indicated a protective mechanism in the roots during Fe uptake.Notably,shoots with a lack of Fe accumulation did not show re-entry of Fe after supplementation,and there was a sustained downregulation of Fe-regulated genes.This suggests that the signaling from roots to shoots influences the response of shoots to Fe supplementation in rice.The molecular changes in Fe homeostasis discovered in this study can contribute to the improvement of rice.
基金financial support received from the Shanghai Oriental Talent Youth Project(T2023218)the National Science Fund for Distinguished Young Scholars of China(32025029)+2 种基金the Young Elite Scientist Sponsorship Program of China Association for Science and Technology(YESS20220128)the Shanghai Research Center for Food Microbiology Engineering(19DZ2281100)the National Natural Science Foundation of China(Key Program)(U23A20261).
文摘Atopic dermatitis(AD),which is associated with inflammatory response and skin damage,is increasing worldwide in recent years.Interest is increasing in the protective effects of natural and fermented products on the prevention and treatment of AD.This study aimed to investigate the ameliorative effects of a new rice milk product fermented with Saccharomyces cerevisiae on AD mice.Fermented rice milk could effectively attenuated the symptoms of AD mice,such as skin lesions,scaliness and lichenification.Furthermore,fermented rice milk decreased the levels of inflammatory cytokines interleukin(IL)-4,IL-6 and tumor necrosis factor(TNF)-αin 2,4-dinitrochlorobenzene(DNCB)induced mice.It was also observed that fermented rice milk treatment downregulated the levels of p-IKBα,p-NF-κB,p-ERK,p-JNK and p-p38 proteins expression in skin of AD mice,which showed the anti-inflammatory function of fermented rice milk,mainly via the NF-κB and MAPK signaling pathways.In addition,S.cerevisiae fermentation reduced original flavor of rice milk,and increased the acid and alcohol compounds.Overall,the findings indicated that S.cerevisiae fermentation improved the flavor characteristics of rice milk,and fermented rice milk exhibited protective effects against DNCB-induced atopic dermatitis by downregulating NF-κB and MAPK signaling pathways and protecting the skin barrier.
基金supported by the National Natural Science Foundation of China(Grant Nos.32201654 and U22A20464)National Key Research and Development Program from the Ministry of Science and Technology of China(Grant No.2020YFA0907600)the 2115 Talent Development Program of China Agricultural University.
文摘Canopy photosynthesis,rather than leaf photosynthesis,is highly related to plant biomass and yield formation.Studying canopy photosynthesis and identifying the parameters that control it can help optimize agricultural management and achieve crop yield potential.Compared with traditional parameters,canopy occupation volume(COV)offers an integrative parameter on canopy architecture related to canopy photosynthetic rates.In this study,we developed a high-throughput method to derive COV for different rice varieties.We first used multi-perspective two-dimensional imaging to reconstruct three-dimensional point clouds of rice plants and developed a suite of pipelines to calculate plant height,leaf number,tiller number,and biomass,with R^(2) values of 91.8%,95.9%,82.3%,and 94.3%,respectively.We further employed point cloud data to reconstruct the surfaces of rice plants and construct a virtual canopy model of the rice population.Light distribution was simulated using a ray-tracing algorithm and canopy photosynthetic rates were simulated via photosynthetic rate-incident light intensity curve fitting.Furthermore,we systematically explored the relationships between canopy phenotypes and photosynthetic rates,and found that COV was the most effective predictor of canopy photosynthesis,achieving an R^(2) value of 92.1%.Adjustment in atmospheric transmittance showed that COV strongly correlated with canopy photosynthesis under different light conditions,with higher accuracy observed under diffuse light.Variations in planting density confirmed that this correlation remained strong at the community level.In summary,this study demonstrates that COV is closely linked to simulated canopy photosynthesis and the developed pipeline can support future agronomic and breeding research.
基金Supported by Natural Science Foundation General Project of Heilongjiang Province(C2018050).
文摘As a vital food crop,rice is an important part of global food crops.Studying the spatiotemporal changes in rice cultivation facilitates early prediction of production risks and provides support for agricultural policy decisions related to rice.With the increasing application of satellite remote sensing technology in crop monitoring,remote sensing for rice cultivation has emerged as a novel approach,offering new perspectives for monitoring rice planting.This paper briefly outlined the current research and development status of satellite remote sensing for monitoring rice cultivation both at home and abroad.Foreign scholars have made innovations in data sources and methodologies for satellite remote sensing monitoring,and utilized multi-source satellite information and machine learning algorithms to enhance the accuracy of rice planting monitoring.Scholars in China have achieved significant results in the study of satellite remote sensing for monitoring rice cultivation.Their research and application in monitoring rice planting areas provide valuable references for agricultural production management.However,satellite remote sensing monitoring of rice still faces challenges such as low spatiotemporal resolution and difficulties related to cloud cover and data fusion,which require further in-depth investigation.Additionally,there are shortcomings in the accuracy of remote sensing monitoring for fragmented farmland plots and smallholder farming.To address these issues,future efforts should focus on developing multi-source heterogeneous data fusion analysis technologies and researching monitoring systems.These advancements are expected to enable high-precision large-scale acquisition of rice planting information,laying a foundation for future smart agriculture.
基金supported by the Hubei Provincial Natural Science Foundation,China(Grant No.2024AFB917).
文摘As sessile organisms,plants must adapt various stresses.Accordingly,they have evolved several plant-specific growth and developmental processes.WRKY53 is a member of the WRKY transcription factor family,which plays a crucial role in rice growth and development,stress response,and hormone signal transduction.This review discusses the role of WRKY53 in stress response,focusing on its functions in cold tolerance,salt tolerance,disease resistance,and pest defense,and explores its role in regulating rice leaf senescence and seed germination.This article also proposes future research directions,including functional genomics studies,protein interaction network analyses,hormone signal transduction pathways,genetic improvement strategies,applications of gene editing technologies,molecular basis of stress responses,cross-species functional conservation,and bioinformatics and comparative genomics research.This review highlights the importance of WRKY53 in rice biology and provides new perspectives and strategies for future research and genetic improvement of rice.
