OBJECTIVE To research the effect of Rhodiolae on cognitive function on dementia rats and to investigate its potential mechanism.METHODS Established dementia model via injecting ibotenic acid into basal nuclei,and rand...OBJECTIVE To research the effect of Rhodiolae on cognitive function on dementia rats and to investigate its potential mechanism.METHODS Established dementia model via injecting ibotenic acid into basal nuclei,and randomly divided the model rats into model group,Aricept group(1.4 mg·kg-1)and Rhodiolae group(0.14 mg·kg-1),moreover the sham group was built byinjecting normal saline.All animals received homologous drugs after modeling 1 week,and the sham group and model group were gave isometric normal saline(10 mL·kg-1),1/d.Morris water maze was used to evaluate the change of cognitive function after the rats were treated 40 d,and pathomorphism in CA1 and cerebral cortex were observed by Nissl staining and the numbers of nerve cell in these areas were counted.The concentration of MDA,H2O2,CAT,SOD and GR in brain tissue were detected by spectrophotography.RESULTS Compared to model group,the escape latency of Rhodiolae groups〔(31.10±20.97)s compare to(51.07±13.04)s〕was shortened but it hadn′t statistical difference,and the total swimming distance〔(8469.88±4724.74)cm compare to(15862.17±1315.69)cm〕was shortened obviously,furthermore the times entered the target quadrant〔(9.50±1.87)times compare to(6.50±1.38)times〕and the percentage in target quadrant〔(33.87±4.47)%compare to(26.17±2.58)%〕were evidently increased.Moderate shrinkage of nucleus of neurons in cerebral cortex of model rats,mild hyperplasia of glial cells and loosely neuron was also observed in CA1 area,but those lesions were distinctly ameliorated in Rhodiolae groups.What′s more,the numbers of nerve cells in CA1〔(70.40±4.00)compare to(45.50±4.95)〕and cerebral cortex〔(63.00±5.29)compare to(42.40±11.87)〕of Rhodiolae groups were obviously increased.In addition,compared to model group,the content of MDA〔(14.72±4.42)compare to(19.99±4.70)g protein·L-1〕and H2O2〔(60.07±6.38)mmol·L-1compare to(76.37±6.06)mmol·L-1〕were evidently decreased,the activity of GSH〔(41.30±14.81)k U·g-1protein compare to(15.93±3.59)k U·g-1protein〕was visibly increased,and the activity of SOD〔(5.74±3.76)g protein·L-1compare to(2.72±1.09)g protein·L-1〕and CAT〔(210.45±60.11)k U·g-1protein compare to(171.48±56.50)k U·g-1protein〕were also increased but without statistical difference.CONCLUSION Rhodiolae improve learning and remembering function,ameliorate pathological changes and protect neuronal loss which respond to decrease the oxidative products and enhance the activity of antioxidase.展开更多
Objective:To explore the effect of rhodiolae treating chronic myocardial infarction with heart failure on left ventricular remodeling and serum inflammatory factors.Methods:A total of 100 cases of chronic myocardial i...Objective:To explore the effect of rhodiolae treating chronic myocardial infarction with heart failure on left ventricular remodeling and serum inflammatory factors.Methods:A total of 100 cases of chronic myocardial infarction with heart failure were selected and randomly divided into treatment group and control group with 50 cases in each group, the control group was treated with strong heart, dehydration, nutrition myocardium, infection prevention and western comprehensive treatment, the treatment group was given rhodiola treatment based on the western medicine treatment, compared the changes of left ventricular remodeling indexes and serum inflammatory factors of two group patients before treatment (T0), 1 months of treatment (T1), 3 months of treatment (T2).Results: (1) There was statistical significance difference at different time points LVEF, LVEDD, LVESD, LVSTD, LVPWTD. LVEF, LVSTD, LVPWTD: T2 > T1 > T0, LVEDD, LVESD: T2 < T1 < T0;In treatment group LVEF, LVSTD and LVPWTD increased, and the decline rate of LVEDD and LVESD was higher than that of control group;(2) There was statistically significant difference in different time points of IL-6, hs-CRP, and NT-proBNP, serum IL-6, hsCRP and NT-proBNP levels: T2 < T1 < T0;The serum IL-6, hs-CRP and NT-proBNP levels of treatment group decreased more than control group.Conclusion: Rhodiolae is helpful to improve the left ventricular remodeling and serum inflammatory factors in patients with chronic myocardial infarction and heart failure.展开更多
In this study,34 deep eutectic solvents(DESs)were successfully prepared for the extraction of proanthocyanidin from Rhodiolae Crenulatae Radix et Rhizomes.The extraction process was optimized using single factor explo...In this study,34 deep eutectic solvents(DESs)were successfully prepared for the extraction of proanthocyanidin from Rhodiolae Crenulatae Radix et Rhizomes.The extraction process was optimized using single factor exploration and Box-Behnken design-response surface analysis.The extraction rate was significantly improved when the molar ratio of choline chloride to 1,3-propanediol was 1:3.5 and the water content was 30%(V/V)in DESs.AB-8 macroporous resin and ethyl acetate were used for separation and refining,and the oligomer-rich proanthocyanidin components were eventually obtained.The ultraviolet(UV)and infrared(IR)spectra showed that the proanthocyanidins were mainly composed of catechin and epicatechin.To further clarify the chemical composition of proanthocyanidin,an ion scan list containing 156 proanthocyanidins precursors was obtained by constructing a proanthocyanidins structural library and mass defect filtering(MDF)algorithm,combined with the full mass spectrometry(MS)/dd-MS2 scan mode that turns on the“if idle pick others”function.By using ultra-high performance liquid chromatography and high-resolution MS(UHPLC/HRMS),the analysis used both targeted and nontargeted methods to detect proanthocyanidins.Finally,50 oligomeric proanthocyanidin(OPC)compounds were identified,including 7 monomers,22 dimers,20 trimers,and 1 tetramer,most of which were procyanidins of proanthocyanidins(84%),and a small amount of prodelphinidin(14%)and other types of proanthocyanidins(2%),which enabled the systematic characterization of proanthocyanidin components from Rhodiolae Crenulatae Radix et Rhizomes.Meanwhile,the comparison with the grape seeds OPCs standard(United States Pharmacopeia)revealed that the proanthocyanidins in Rhodiolae Crenulatae Radix et Rhizomes were more abundant,suggesting that the proanthocyanidins in Rhodiolae Crenulatae Radix et Rhizomes has promising applications.展开更多
Chemical constituents of the whole herb of Rhodiola kirilowii(Regl) Maxim.were investigated.The separation of the constituents was achieved by using a combination of various chromatographic techniques including column...Chemical constituents of the whole herb of Rhodiola kirilowii(Regl) Maxim.were investigated.The separation of the constituents was achieved by using a combination of various chromatographic techniques including column chromatography over silica gel,ODS,Sephadex LH-20 and reversed-phase HPLC.Their structures were elucidated by spectroscopic techniques including 1D and 2D NMR spectroscopy.Eleven compounds were obtained and identified as 4-hydroxyphenylethyl-(4'- methoxyphenylethyl)ether(1),1-(2-hydroxy-2-methylbutanoate)-β-D-glucopyranose(2),4-ethoxy-phenylethanol acetate(3),p-hydroxyacetophenone (4),p-hydroxy-benzoic acid ethylester(5),4-hydroxybenzoic acid(6),4-hydroxybenzaldehyde(7),R(-)-mellein (8),stigmasterol(9),4-methoxy-phenylethanol(10),and methylgallate(11).Among them,1 and 2 are new compounds; compounds 3,5,7,8,and 10 were isolated from Rhodiola genus for the first time;and compounds 4,6,9,and 11 were isolated from Rhodiola kirilowii(Regl) Maxim.for the first time.展开更多
[Objective] The study aimed to analyze the ITS sequences of nrDNA from Rhodiola alisa and investigate the difference of evolution rate between nrDNA and trnS-trnG and rpl20-rps12 sequences of cpDNA(chloroplast DNA).[M...[Objective] The study aimed to analyze the ITS sequences of nrDNA from Rhodiola alisa and investigate the difference of evolution rate between nrDNA and trnS-trnG and rpl20-rps12 sequences of cpDNA(chloroplast DNA).[Method]Total DNA was extracted from silica-dried leaves of R.alsia by using modified CTAB method.With the extracted DNA sample as template,nrDNA ITS region was amplified,then purified and sequenced.In addition,the yielded ITS sequences were also compared with the known trnS-trnG and rpl20-rps12 sequences of cpDNA from R.alsia.[Result]The ITS sequence of nrDNA from R.alsia was 701 bp in length,of which 13 variable sites were found with a percentage of 1.85%.Of the 13 variable sites,8 were caused by point mutations,5 were the results of insertions or deletions.The(A+T)content and(G+C)content were 46.9% and 53.1%,respectively.The nucleotide diversity(π)was 0.004 27.[Conclusion]The ITS region of nrDNA from R.alsia was more conservative and evolved more slowly than the trnS-trnG and rpl20-rps12 sequences of its cpDNA.展开更多
The orthogonal experimental design was used to optimize the formula of maca lozenge and explore the preparation techniques and product property of maca lozenges. With the sensory evaluation of maca lozenge as the indi...The orthogonal experimental design was used to optimize the formula of maca lozenge and explore the preparation techniques and product property of maca lozenges. With the sensory evaluation of maca lozenge as the indicator, L16 （44） orthogonal design graph was used to select the optimal techniques, and to investigate the stability, heavy metals and microorganisms contents in the tablets. The optimal formula was 80% of maca power, 12% of Rhodiola rosea powder, and 6% of Angelica sinensis powder, where the comprehensive sensory index reached 246. Moreover, under the optimal conditions, various kinds of heavy metals contents were in consistent with the GB164740 standards, and there was no significant change in color, size, friability, and disintegration time, as well as microorganism content during the 12-month preservation. With simple, easy and reliable technique and quality control method, the process study had obtained the national patent process （patent number： ZL200810233796.X）, and the contents of heavy metals and micr6organisms laid foundation for the quality standard.展开更多
The Rhodiola genus（Crassulaceae） is composed of about 90 species, mainly growing in high and cold regions. Some species are used as medicinal herbs to treat many diseases, such as cerebral hypoxia, cardiovascular di...The Rhodiola genus（Crassulaceae） is composed of about 90 species, mainly growing in high and cold regions. Some species are used as medicinal herbs to treat many diseases, such as cerebral hypoxia, cardiovascular disease, plateau response and so on. Up to now, about 180 constituents which are mainly glycosides, flavonoids, terpenoids, tannins, sterol and other compounds have been identified in the genus. The present review summarizes the chemical constituents isolated from the Rhodiola genus over the past few decades, with particular emphasis on Rhodiola crenulata（HK. f. et Thoms.） H. Ohba which is being studied by our research group.展开更多
A new glucoside, fastigitin A (1), namely 2-O-β-D-glucopyransyl-3-methyl-methyl pinalate, together with twelve known constituents (2-13), was isolated from the root of Rhodiola fastigiata (Hook. f. et Thoms.) S. H....A new glucoside, fastigitin A (1), namely 2-O-β-D-glucopyransyl-3-methyl-methyl pinalate, together with twelve known constituents (2-13), was isolated from the root of Rhodiola fastigiata (Hook. f. et Thoms.) S. H. Fu collected from Nujiang Lisu autonomous region, Yunnan, China. Their structures were identified by spectral (including 2D-NMR techniques) and chemical methods. Compounds 2 and 5-9 were obtained from this plant for the first time.展开更多
Objective To investigate the pretreatment effects of Rhodiola rosea （R. rosea） extract on cognitive dysfunction, oxidative stress in hippocampus and hippocampal neuron injury in a rat model of Alzheimer＇s disease ...Objective To investigate the pretreatment effects of Rhodiola rosea （R. rosea） extract on cognitive dysfunction, oxidative stress in hippocampus and hippocampal neuron injury in a rat model of Alzheimer＇s disease （AD）. Methods Male Sprague-Dawley rats were pretreated with R. rosea extract at doses of 1.5, 3.0, and 6.0 g/kg for 3 weeks, followed by bilateral intracerebroventricular injection with streptozotocin （1.5 mg/kg） on days 1 and 3. Behavioral alterations were monitored after 2 weeks from the lesion using Morris water maze task. Three weeks after the lesion, the rats were sacrificed for measuring the malondialdehyde （MDA）, glutathione reductase （GR） and reduced glutathione （GSH） levels in hippocampus and histopathology of hippocampal neurons. Results The MDA level was significantly increased while the GR and GSH levels were significantly decreased with striking impairments in spatial learning and memory and severe damage to hippocampal neurons in the model rat induced by intracerebroventricular injection of streptozotocin. These abnormalities were significantly improved by pretreatment with R. rosea extract （3.0 g/kg）. Conclusion R. rosea extract can protect rats against cognitive deficits, neuronal injury and oxidative stress induced by intracerebroventricular injection of streptozotocin, and may be used as a potential agent in treatment of neurodegenerative diseases such as AD.展开更多
Objective:To investigate the in vitro antioxidant and antiproliferative activity of rhizome extracts of Rhodiola imbricata(R.imbricata) in HT-29 human colon cancer cell line.Methods:The successively extracted rhizome ...Objective:To investigate the in vitro antioxidant and antiproliferative activity of rhizome extracts of Rhodiola imbricata(R.imbricata) in HT-29 human colon cancer cell line.Methods:The successively extracted rhizome of R.imbricata using various solvents was analyzed for their total phenolics,tannins and flavonoid contents.In vitro antioxidant activity was evaluated by employing different assays,including DPPH,ABTS radical scavenging assays,FRAP, phosphomolybdenum reduction assay,superoxide anion,hydroxyl radical scavenging activities and metal chelating ability.Results:Acetone and methanol extracts recorded higher phenolic content and showed comparable antioxidant activity with standard reference.Additionally,they also inhibited the proliferation of HT-29 cells upon treatment at higher concentration(200μg/mL) (acetone and methanol,84%and 84%.respectively).On examination acetone extract exhibited antiproliferative activity in a concentration dependent manner whereas,methanol extract showed both dose dependent and lime dependent inhibitory activity.Conclusions:The results obtained justify the traditional usage of R.imbricata from their promising antioxidant activity.展开更多
Rhodioloside has been shown to protect cells from hypoxia injury,and bone marrow mesenchymal stem cells have a good effect on tissue repair.To study the effects of rhodioloside and bone marrow mesenchymal stem cells o...Rhodioloside has been shown to protect cells from hypoxia injury,and bone marrow mesenchymal stem cells have a good effect on tissue repair.To study the effects of rhodioloside and bone marrow mesenchymal stem cells on spinal cord injury,a rat model of spinal cord injury was established using the Infinite Horizons method.After establishing the model,the rats were randomly divided into five groups.Rats in the control group were intragastrically injected with phosphate buffered saline(PBS)(5μL).PBS was injected at 6 equidistant points around 5 mm from the injury site and at a depth of 5 mm.Rats in the rhodioloside group were intragastrically injected with rhodioloside(5 g/kg)and intramuscularly injected with PBS.Rats in the mesenchymal stem cell(MSC)group were intramuscularly injected with PBS and intramuscularly with MSCs(8×10^6/mL in a 50-μL cell suspension).Rats in the Ad-HIF-MSC group were intragastrically injected with PBS and intramuscularly injected with HIF-1 adenovirus-infected MSCs.Rats in the rhodioloside+Ad-HIF-MSC group were intramuscularly injected with MSCs infected with the HIF-1 adenovirus and intragastrically injected with rhodioloside.One week after treatment,exercise recovery was evaluated with a modified combined behavioral score scale.Hematoxylin-eosin staining and Pischingert’s methylene blue staining were used to detect any histological or pathological changes in spinal cord tissue.Levels of adenovirus IX and Sry mRNA were detected by real-time quantitative polymerase chain reaction and used to determine the number of adenovirus and mesenchymal stem cells that were transfected into the spinal cord.Immunohistochemical staining was applied to detect HIF-1 protein levels in the spinal cord.The results showed that:(1)compared with the other groups,the rhodioloside+Ad-HIF-MSC group exhibited the highest combined behavioral score(P<0.05),the most recovered tissue,and the greatest number of neurons,as indicated by Pischingert’s methylene blue staining.(2)Compared with the PBS group,HIF-1 protein expression was greater in the rhodioloside group(P<0.05).(3)Compared with the Ad-HIF-MSC group,Sry mRNA levels were higher in the rhodioloside+Ad-HIF-MSC group(P<0.05).These results confirm that rhodioloside combined with bone marrow mesenchymal stem cells can promote the recovery of spinal cord injury and activate the HIF-1 pathway to promote the survival of bone marrow mesenchymal stem cells and repair damaged neurons within spinal cord tissue.This experiment was approved by the Animal Ethics Committee of Gansu University of Traditional Chinese Medicine,China(approval No.2015KYLL029)in June 2015.展开更多
基金The project supported by Key Projects of National Natural Science Foundation of China(81430100)Academic and technical leaders of Sichuan Province to Raise Funds for Funding Projects(2015058)Sichuan Province Youth Science and technology Innovation Research Team(2014TD0007)
文摘OBJECTIVE To research the effect of Rhodiolae on cognitive function on dementia rats and to investigate its potential mechanism.METHODS Established dementia model via injecting ibotenic acid into basal nuclei,and randomly divided the model rats into model group,Aricept group(1.4 mg·kg-1)and Rhodiolae group(0.14 mg·kg-1),moreover the sham group was built byinjecting normal saline.All animals received homologous drugs after modeling 1 week,and the sham group and model group were gave isometric normal saline(10 mL·kg-1),1/d.Morris water maze was used to evaluate the change of cognitive function after the rats were treated 40 d,and pathomorphism in CA1 and cerebral cortex were observed by Nissl staining and the numbers of nerve cell in these areas were counted.The concentration of MDA,H2O2,CAT,SOD and GR in brain tissue were detected by spectrophotography.RESULTS Compared to model group,the escape latency of Rhodiolae groups〔(31.10±20.97)s compare to(51.07±13.04)s〕was shortened but it hadn′t statistical difference,and the total swimming distance〔(8469.88±4724.74)cm compare to(15862.17±1315.69)cm〕was shortened obviously,furthermore the times entered the target quadrant〔(9.50±1.87)times compare to(6.50±1.38)times〕and the percentage in target quadrant〔(33.87±4.47)%compare to(26.17±2.58)%〕were evidently increased.Moderate shrinkage of nucleus of neurons in cerebral cortex of model rats,mild hyperplasia of glial cells and loosely neuron was also observed in CA1 area,but those lesions were distinctly ameliorated in Rhodiolae groups.What′s more,the numbers of nerve cells in CA1〔(70.40±4.00)compare to(45.50±4.95)〕and cerebral cortex〔(63.00±5.29)compare to(42.40±11.87)〕of Rhodiolae groups were obviously increased.In addition,compared to model group,the content of MDA〔(14.72±4.42)compare to(19.99±4.70)g protein·L-1〕and H2O2〔(60.07±6.38)mmol·L-1compare to(76.37±6.06)mmol·L-1〕were evidently decreased,the activity of GSH〔(41.30±14.81)k U·g-1protein compare to(15.93±3.59)k U·g-1protein〕was visibly increased,and the activity of SOD〔(5.74±3.76)g protein·L-1compare to(2.72±1.09)g protein·L-1〕and CAT〔(210.45±60.11)k U·g-1protein compare to(171.48±56.50)k U·g-1protein〕were also increased but without statistical difference.CONCLUSION Rhodiolae improve learning and remembering function,ameliorate pathological changes and protect neuronal loss which respond to decrease the oxidative products and enhance the activity of antioxidase.
文摘Objective:To explore the effect of rhodiolae treating chronic myocardial infarction with heart failure on left ventricular remodeling and serum inflammatory factors.Methods:A total of 100 cases of chronic myocardial infarction with heart failure were selected and randomly divided into treatment group and control group with 50 cases in each group, the control group was treated with strong heart, dehydration, nutrition myocardium, infection prevention and western comprehensive treatment, the treatment group was given rhodiola treatment based on the western medicine treatment, compared the changes of left ventricular remodeling indexes and serum inflammatory factors of two group patients before treatment (T0), 1 months of treatment (T1), 3 months of treatment (T2).Results: (1) There was statistical significance difference at different time points LVEF, LVEDD, LVESD, LVSTD, LVPWTD. LVEF, LVSTD, LVPWTD: T2 > T1 > T0, LVEDD, LVESD: T2 < T1 < T0;In treatment group LVEF, LVSTD and LVPWTD increased, and the decline rate of LVEDD and LVESD was higher than that of control group;(2) There was statistically significant difference in different time points of IL-6, hs-CRP, and NT-proBNP, serum IL-6, hsCRP and NT-proBNP levels: T2 < T1 < T0;The serum IL-6, hs-CRP and NT-proBNP levels of treatment group decreased more than control group.Conclusion: Rhodiolae is helpful to improve the left ventricular remodeling and serum inflammatory factors in patients with chronic myocardial infarction and heart failure.
基金supported by the Science and Technology Program of Tianjin in China(Grant No.:23ZYJDSS00030)the Science and Technology Project of Haihe Laboratory of Modern Chinese Medicine,China(Grant Nos.:22HHZYSS00007 and 22HHZYJC00003).
文摘In this study,34 deep eutectic solvents(DESs)were successfully prepared for the extraction of proanthocyanidin from Rhodiolae Crenulatae Radix et Rhizomes.The extraction process was optimized using single factor exploration and Box-Behnken design-response surface analysis.The extraction rate was significantly improved when the molar ratio of choline chloride to 1,3-propanediol was 1:3.5 and the water content was 30%(V/V)in DESs.AB-8 macroporous resin and ethyl acetate were used for separation and refining,and the oligomer-rich proanthocyanidin components were eventually obtained.The ultraviolet(UV)and infrared(IR)spectra showed that the proanthocyanidins were mainly composed of catechin and epicatechin.To further clarify the chemical composition of proanthocyanidin,an ion scan list containing 156 proanthocyanidins precursors was obtained by constructing a proanthocyanidins structural library and mass defect filtering(MDF)algorithm,combined with the full mass spectrometry(MS)/dd-MS2 scan mode that turns on the“if idle pick others”function.By using ultra-high performance liquid chromatography and high-resolution MS(UHPLC/HRMS),the analysis used both targeted and nontargeted methods to detect proanthocyanidins.Finally,50 oligomeric proanthocyanidin(OPC)compounds were identified,including 7 monomers,22 dimers,20 trimers,and 1 tetramer,most of which were procyanidins of proanthocyanidins(84%),and a small amount of prodelphinidin(14%)and other types of proanthocyanidins(2%),which enabled the systematic characterization of proanthocyanidin components from Rhodiolae Crenulatae Radix et Rhizomes.Meanwhile,the comparison with the grape seeds OPCs standard(United States Pharmacopeia)revealed that the proanthocyanidins in Rhodiolae Crenulatae Radix et Rhizomes were more abundant,suggesting that the proanthocyanidins in Rhodiolae Crenulatae Radix et Rhizomes has promising applications.
基金National Natural Science Foundation of China (Grant No.30973628).
文摘Chemical constituents of the whole herb of Rhodiola kirilowii(Regl) Maxim.were investigated.The separation of the constituents was achieved by using a combination of various chromatographic techniques including column chromatography over silica gel,ODS,Sephadex LH-20 and reversed-phase HPLC.Their structures were elucidated by spectroscopic techniques including 1D and 2D NMR spectroscopy.Eleven compounds were obtained and identified as 4-hydroxyphenylethyl-(4'- methoxyphenylethyl)ether(1),1-(2-hydroxy-2-methylbutanoate)-β-D-glucopyranose(2),4-ethoxy-phenylethanol acetate(3),p-hydroxyacetophenone (4),p-hydroxy-benzoic acid ethylester(5),4-hydroxybenzoic acid(6),4-hydroxybenzaldehyde(7),R(-)-mellein (8),stigmasterol(9),4-methoxy-phenylethanol(10),and methylgallate(11).Among them,1 and 2 are new compounds; compounds 3,5,7,8,and 10 were isolated from Rhodiola genus for the first time;and compounds 4,6,9,and 11 were isolated from Rhodiola kirilowii(Regl) Maxim.for the first time.
基金Supported by National Natural Science Foundation of China(30170066,30370284)National Basic Research Program of China(2008CB117013)International Foundation for Science(IFSD4258-1)~~
文摘[Objective] The study aimed to analyze the ITS sequences of nrDNA from Rhodiola alisa and investigate the difference of evolution rate between nrDNA and trnS-trnG and rpl20-rps12 sequences of cpDNA(chloroplast DNA).[Method]Total DNA was extracted from silica-dried leaves of R.alsia by using modified CTAB method.With the extracted DNA sample as template,nrDNA ITS region was amplified,then purified and sequenced.In addition,the yielded ITS sequences were also compared with the known trnS-trnG and rpl20-rps12 sequences of cpDNA from R.alsia.[Result]The ITS sequence of nrDNA from R.alsia was 701 bp in length,of which 13 variable sites were found with a percentage of 1.85%.Of the 13 variable sites,8 were caused by point mutations,5 were the results of insertions or deletions.The(A+T)content and(G+C)content were 46.9% and 53.1%,respectively.The nucleotide diversity(π)was 0.004 27.[Conclusion]The ITS region of nrDNA from R.alsia was more conservative and evolved more slowly than the trnS-trnG and rpl20-rps12 sequences of its cpDNA.
基金Supported by the Special Project for the National Basic Work of Science and Technology(2006FY110700)the Provincial Plans for the Scientific and Technological Innovation of Yunnan Province(2007C0219Z)the Special Fund for the Provincial Financial Development of Bio-industry of Yunnan Province(Yuncainong[2011]274)~~
文摘The orthogonal experimental design was used to optimize the formula of maca lozenge and explore the preparation techniques and product property of maca lozenges. With the sensory evaluation of maca lozenge as the indicator, L16 （44） orthogonal design graph was used to select the optimal techniques, and to investigate the stability, heavy metals and microorganisms contents in the tablets. The optimal formula was 80% of maca power, 12% of Rhodiola rosea powder, and 6% of Angelica sinensis powder, where the comprehensive sensory index reached 246. Moreover, under the optimal conditions, various kinds of heavy metals contents were in consistent with the GB164740 standards, and there was no significant change in color, size, friability, and disintegration time, as well as microorganism content during the 12-month preservation. With simple, easy and reliable technique and quality control method, the process study had obtained the national patent process （patent number： ZL200810233796.X）, and the contents of heavy metals and micr6organisms laid foundation for the quality standard.
基金Tianjin City High School Science & Technology Fund Planning Project(Grant No.20130202)
文摘The Rhodiola genus（Crassulaceae） is composed of about 90 species, mainly growing in high and cold regions. Some species are used as medicinal herbs to treat many diseases, such as cerebral hypoxia, cardiovascular disease, plateau response and so on. Up to now, about 180 constituents which are mainly glycosides, flavonoids, terpenoids, tannins, sterol and other compounds have been identified in the genus. The present review summarizes the chemical constituents isolated from the Rhodiola genus over the past few decades, with particular emphasis on Rhodiola crenulata（HK. f. et Thoms.） H. Ohba which is being studied by our research group.
文摘A new glucoside, fastigitin A (1), namely 2-O-β-D-glucopyransyl-3-methyl-methyl pinalate, together with twelve known constituents (2-13), was isolated from the root of Rhodiola fastigiata (Hook. f. et Thoms.) S. H. Fu collected from Nujiang Lisu autonomous region, Yunnan, China. Their structures were identified by spectral (including 2D-NMR techniques) and chemical methods. Compounds 2 and 5-9 were obtained from this plant for the first time.
文摘Objective To investigate the pretreatment effects of Rhodiola rosea （R. rosea） extract on cognitive dysfunction, oxidative stress in hippocampus and hippocampal neuron injury in a rat model of Alzheimer＇s disease （AD）. Methods Male Sprague-Dawley rats were pretreated with R. rosea extract at doses of 1.5, 3.0, and 6.0 g/kg for 3 weeks, followed by bilateral intracerebroventricular injection with streptozotocin （1.5 mg/kg） on days 1 and 3. Behavioral alterations were monitored after 2 weeks from the lesion using Morris water maze task. Three weeks after the lesion, the rats were sacrificed for measuring the malondialdehyde （MDA）, glutathione reductase （GR） and reduced glutathione （GSH） levels in hippocampus and histopathology of hippocampal neurons. Results The MDA level was significantly increased while the GR and GSH levels were significantly decreased with striking impairments in spatial learning and memory and severe damage to hippocampal neurons in the model rat induced by intracerebroventricular injection of streptozotocin. These abnormalities were significantly improved by pretreatment with R. rosea extract （3.0 g/kg）. Conclusion R. rosea extract can protect rats against cognitive deficits, neuronal injury and oxidative stress induced by intracerebroventricular injection of streptozotocin, and may be used as a potential agent in treatment of neurodegenerative diseases such as AD.
基金supported by the DIHAR,Defense Research Development Organization Indiathe Food Security Center,University of Hohenheim,Stuttgart,Germany for awarding the Sandwich Scholarship
文摘Objective:To investigate the in vitro antioxidant and antiproliferative activity of rhizome extracts of Rhodiola imbricata(R.imbricata) in HT-29 human colon cancer cell line.Methods:The successively extracted rhizome of R.imbricata using various solvents was analyzed for their total phenolics,tannins and flavonoid contents.In vitro antioxidant activity was evaluated by employing different assays,including DPPH,ABTS radical scavenging assays,FRAP, phosphomolybdenum reduction assay,superoxide anion,hydroxyl radical scavenging activities and metal chelating ability.Results:Acetone and methanol extracts recorded higher phenolic content and showed comparable antioxidant activity with standard reference.Additionally,they also inhibited the proliferation of HT-29 cells upon treatment at higher concentration(200μg/mL) (acetone and methanol,84%and 84%.respectively).On examination acetone extract exhibited antiproliferative activity in a concentration dependent manner whereas,methanol extract showed both dose dependent and lime dependent inhibitory activity.Conclusions:The results obtained justify the traditional usage of R.imbricata from their promising antioxidant activity.
基金supported by the National High Technology Research and Development Program of China (863 Program), No. 2015CB755400 (to XQH)
文摘Rhodioloside has been shown to protect cells from hypoxia injury,and bone marrow mesenchymal stem cells have a good effect on tissue repair.To study the effects of rhodioloside and bone marrow mesenchymal stem cells on spinal cord injury,a rat model of spinal cord injury was established using the Infinite Horizons method.After establishing the model,the rats were randomly divided into five groups.Rats in the control group were intragastrically injected with phosphate buffered saline(PBS)(5μL).PBS was injected at 6 equidistant points around 5 mm from the injury site and at a depth of 5 mm.Rats in the rhodioloside group were intragastrically injected with rhodioloside(5 g/kg)and intramuscularly injected with PBS.Rats in the mesenchymal stem cell(MSC)group were intramuscularly injected with PBS and intramuscularly with MSCs(8×10^6/mL in a 50-μL cell suspension).Rats in the Ad-HIF-MSC group were intragastrically injected with PBS and intramuscularly injected with HIF-1 adenovirus-infected MSCs.Rats in the rhodioloside+Ad-HIF-MSC group were intramuscularly injected with MSCs infected with the HIF-1 adenovirus and intragastrically injected with rhodioloside.One week after treatment,exercise recovery was evaluated with a modified combined behavioral score scale.Hematoxylin-eosin staining and Pischingert’s methylene blue staining were used to detect any histological or pathological changes in spinal cord tissue.Levels of adenovirus IX and Sry mRNA were detected by real-time quantitative polymerase chain reaction and used to determine the number of adenovirus and mesenchymal stem cells that were transfected into the spinal cord.Immunohistochemical staining was applied to detect HIF-1 protein levels in the spinal cord.The results showed that:(1)compared with the other groups,the rhodioloside+Ad-HIF-MSC group exhibited the highest combined behavioral score(P<0.05),the most recovered tissue,and the greatest number of neurons,as indicated by Pischingert’s methylene blue staining.(2)Compared with the PBS group,HIF-1 protein expression was greater in the rhodioloside group(P<0.05).(3)Compared with the Ad-HIF-MSC group,Sry mRNA levels were higher in the rhodioloside+Ad-HIF-MSC group(P<0.05).These results confirm that rhodioloside combined with bone marrow mesenchymal stem cells can promote the recovery of spinal cord injury and activate the HIF-1 pathway to promote the survival of bone marrow mesenchymal stem cells and repair damaged neurons within spinal cord tissue.This experiment was approved by the Animal Ethics Committee of Gansu University of Traditional Chinese Medicine,China(approval No.2015KYLL029)in June 2015.
