Atrophic rhinitis is a disease which manifests itself mainly by anosmia due to dryness and atrophy of the nasal mucosa. There is no specific therapy for the disease at present. In the past few years, 23 cases of atrop...Atrophic rhinitis is a disease which manifests itself mainly by anosmia due to dryness and atrophy of the nasal mucosa. There is no specific therapy for the disease at present. In the past few years, 23 cases of atrophic rhinitis were treated mainly by deep puncture at three points in the nasal region with satisfactory results. In order to find out the functional changes of the nasal mucosa, the mucociliary transport rate (MTR), surface temperature of the conchal mucosa, acid-base scale of nasal secretion, and volume of nasal secretion were determined before and after the treatment.展开更多
BACKGROUND Chronic atrophic gastritis(CAG)is a clinically refractory gastric disease often characterized by high recurrence rates and adverse drug reactions.Anwei decoction(AWD),a traditional Chinese medicine formula,...BACKGROUND Chronic atrophic gastritis(CAG)is a clinically refractory gastric disease often characterized by high recurrence rates and adverse drug reactions.Anwei decoction(AWD),a traditional Chinese medicine formula,has been shown to significantly improve clinical symptoms in patients with CAG,as demonstrated by a multicenter cohort study(overall effective rate:82.5%,P<0.01).However,the unclear molecular mechanisms and therapeutic targets of AWD limit its international acceptance.AIM To investigate the therapeutic mechanisms of AWD against CAG from an integrated perspective.METHODS In this study,N-methyl-N’-nitro-N-nitrosoguanidine was used to establish a CAG rat model.Serum-derived constituents transferred from AWD were first identified using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry.The concentrations of inflammatory cytokines in serum samples were determined by enzyme-linked immunosorbent assay.Moreover,gastric mucosal tissues were analyzed by quantitative realtime polymerase chain reaction to measure messenger RNA(mRNA)levels of the NLRP3 inflammasome.Western blotting was used to detect the protein expression of NLRP3,caspase-1,and interleukin(IL)-1β.To elucidate the regulatory mechanisms underlying AWD treatment,structural alterations of the gut microbiota(GM)and associated metabolites were analyzed using integrated high-throughput sequencing(16S rRNA)and liquid chromatography-mass spectrometry based untargeted metabolomics.This comprehensive approach systematically clarified AWD’s multi-target therapeutic mechanisms against CAG.RESULTS AWD notably reduced serum levels of pro-inflammatory cytokines,such as IL-1β,IL-18,tumor necrosis factor-α,and lipopolysaccharide,demonstrating significant statistical differences(all P<0.01).Additionally,AWD substantially inhibited NLRP3 mRNA expression in gastric mucosal tissue(P<0.01)and concurrently decreased the protein abundance of NLRP3,IL-1β,and caspase-1(all P<0.01),thereby suppressing inflammasome signaling activation.GM analysis indicated that AWD intervention significantly increased the relative abundance of beneficial bacteria.Associated microbial metabolites likely inhibited the NLRP3 inflammasome pathway by modulating immune cell function.Non-targeted metabolomics further indicated that AWD exerted anti-inflammatory effects by regulating critical metabolic pathways,including the Kaposi’s sarcoma-associated herpesvirus infection pathway,autophagy processes,and glycosylphosphatidylinositol-anchor biosynthesis.CONCLUSION AWD alleviates the pathological progression of CAG through multi-target synergistic mechanisms.On one hand,AWD directly suppresses gastric mucosal inflammation by inhibiting NLRP3 inflammasome activation.On the other hand,AWD remodels intestinal microbiota-metabolite homeostasis,enhances intestinal barrier function,and regulates mucosal immune responses.展开更多
Background:The development of gastric cancer(GC)encompasses precancerous conditions like chronic atrophic gastritis(CAG)and premalignant lesions of gastric cancer(PLGC).In these situations,abnormal Notch signaling res...Background:The development of gastric cancer(GC)encompasses precancerous conditions like chronic atrophic gastritis(CAG)and premalignant lesions of gastric cancer(PLGC).In these situations,abnormal Notch signaling results in mucosal impairment and the initiation of cancer.Banxia Xiexin Decoction(BXD),a well-known formula in traditional Chinese medicine(TCM),shows promise in treating gastric disorders,but its mechanisms in gastric restoration remain unclear.Methods:Using MNNG-induced CAG and PLGC rat models,BXD was administered for 12 weeks.Gastric mucosal pathology was assessed via hematoxylin-eosin staining.Proliferation(Ki-67)and angiogenesis(VEGFA)markers were evaluated by immunohistochemistry.Network pharmacology identified BXD’s targets and pathways.Notch pathway components(Notch1,Jagged1,Dll4,Hes1)were analyzed via qPCR,Western blot,and immunohistochemistry.Results:BXD significantly ameliorated mucosal atrophy,glandular structural disorder,and dysplasia in CAG and PLGC rats.Network pharmacology revealed 323 overlapping targets between BXD and PLGC,with Notch signaling as a central pathway.BXD downregulated Notch1,Jagged1,Dll4,and Hes1 expression at transcriptional and protein levels,suppressed Ki-67(proliferation)and VEGFA(angiogenesis)overexpression,and restored gastric mucosal integrity.Conclusion:BXD inhibits Notch signaling,reduces aberrant proliferation and angiogenesis,and interrupts Correa’s gastric carcinogenesis cascade.This study provides mechanistic evidence supporting BXD as a TCM-based intervention for gastric precancerous lesions.展开更多
This study makes an in-depth exploration of the core pathogenesis of chronic atrophic gastritis,Qi Deficiency with Stagnation,to systematically interpret it within the theoretical framework of Achieving Central Harmon...This study makes an in-depth exploration of the core pathogenesis of chronic atrophic gastritis,Qi Deficiency with Stagnation,to systematically interpret it within the theoretical framework of Achieving Central Harmony,and to provide a treatment plan.Professor Li Tingquan believes that the occurrence and development of chronic atrophic gastritis(CAG)is fundamentally a process of harmony imbalance of the spleen and stomach.Its specific manifestation lies in the interaction between qi deficiency and stagnation,namely,spleen deficiency as the root cause,and qi stagnation,phlegm-dampness,and blood stasis obstructing the middle energizer as the secondary manifestations.These factors are mutually causal and interact with one another.Based on this,he proposed a four-step method to treat the disease:eliminating pathogenic factors,harmonizing the spleen and stomach,activating blood circulation to resolve stasis,and tonifying the kidney to generate blood(metaphorically described as"closing the mountains to cultivate forests and thicken the soil,breeding seeds and irrigating for stomach recovery").This approach aims to restore the harmonious state of the middle energizer,providing a complete clinical framework of theory,principle,formula,and herbs for the prevention and treatment of CAG.展开更多
Chronic atrophic gastritis(CAG)is an important stage of precancerous lesions of gastric cancer.Effective treatment and regulation of CAG are essential to prevent its progression to malignancy.Traditional Chinese medic...Chronic atrophic gastritis(CAG)is an important stage of precancerous lesions of gastric cancer.Effective treatment and regulation of CAG are essential to prevent its progression to malignancy.Traditional Chinese medicine(TCM)has shown multi-targeted efficacy in CAG treatment,with advantages in enhancing gastric mucosal barrier defense,improving microcirculation,modulating inflammatory and immune responses,and promoting lesion healing,etc.Clinical studies and meta-analyses indicate that TCM provides significant benefits,with specific Chinese herbal compounds and monomers demonstrating protective effects on the gastric mucosa through mechanisms including anti-inflammation,antioxidation,and regulation of cellular proliferation and apoptosis,etc.Finally,it is pointed out that the efficacy of TCM in the treatment of CAG requires standardized research and unified standards,and constantly clarifies and improves the evaluation criteria of each dimension of gastric mucosal barrier function.展开更多
OBJECTIVE:To explore the correlation between acupuncture treatment for allergic rhinitis(AR) and hypothalamic-pituitary-adrenal(HPA) axis regulation by investigating changes in serum immune factors,HPA axis-associated...OBJECTIVE:To explore the correlation between acupuncture treatment for allergic rhinitis(AR) and hypothalamic-pituitary-adrenal(HPA) axis regulation by investigating changes in serum immune factors,HPA axis-associated hormone levels,activation levels of paraventricular nucleus(PVN) neurons,and the severity of nasal mucosal lesions,in rats with AR before and after acupuncture treatment.METHODS:After establishing the AR rat model,ovalbumin was administered via nasal drip to all groups except the blank control.Each group received continuous treatment for 14 d:the acupuncture,acupuncture + RU-486(mifepristone),and RU-486 groups received acupuncture only,RU486 intraperitoneal injection and acupuncture,and RU-486 intraperitoneal injection only,respectively.Following the intervention period,behavioral scoring was performed on all AR rats,and peripheral blood,nasal mucosa samples,and brains tissue(containing PVN region) were obtained following euthanization.Interleukin(IL-4,IL-5,IL-13),interferon gamma(IFN-γ),corticosterone(CORT),and corticotrophin-releasing hormone(CRH) levels were evaluated in peripheral blood samples.Adrenocorticotropic hormone(ACTH) levels were determined using an enzyme-linked immunoassay assay,and hematoxylin and eosin staining was performed on the nasal mucosa samples.The expression levels of c-Fos in PVN neurons following acupuncture treatment were evaluated by immunofluorescent staining.RESULTS:Following the intervention period,the behavioral scores for the blank control group were lower than those of other groups(P < 0.05),while the acupuncture group scores were lower than those in the model control,acupuncture + RU486,and RU486 groups(P < 0.05).The blank control group had lower serum IL-4,IL-5,and IL-13 levels than those in the other groups(P < 0.05).The acupuncture group had lower serum IL-4,IL-5,and IL-13 levels than those in the model control,acupuncture + RU486,and RU486 groups(P < 0.05).The blank control group had the highest serum IFN-γ levels among all groups,followed by the acupuncture group.The serum CORT,CRH,and ACTH levels in the blank control group were lower than those in the remaining groups(P < 0.05).These biomarker levels were also lower in the acupuncture group than those in the model control,acupuncture + RU486,and RU486 groups(P < 0.05).Compared with model,rats in the acupuncture group exhibited an increased c-Fos expression in PVN neurons.CONCLUSION:Acupuncture can alleviate AR symptoms and regulate serum inflammatory factor levels and HPA axis-related hormones in AR rats.Moreover,these effects are inhibited by glucocorticoid antagonists,suggesting that acupuncture may regulate AR symptoms through HPA axis regulation.展开更多
Sophorae Flavescentis Radix(Ku Shen)is a traditional Chinese medicine used to treat damp-heat syndrome-related diseases,such as dysentery,jaundice,rhinitis,and skin inflammation.Recent studies have revealed the potent...Sophorae Flavescentis Radix(Ku Shen)is a traditional Chinese medicine used to treat damp-heat syndrome-related diseases,such as dysentery,jaundice,rhinitis,and skin inflammation.Recent studies have revealed the potential pharmacological value of its active alkaloid components,matrine(MAT)and oxymatrine(OMT),in the treatment of allergic rhinitis(AR).To develop a thermosensitive in-situ gel containing MAT and OMT for the intranasal treatment of AR,a thermosensitive matrix composed of Pluronic F127/F68 was optimized using the Box-Behnken Response Surface Methodology.The biosafety of the formulation was evaluated using a palatal ciliary movement model.A guinea pig model of AR induced by ovalbumin and aluminum hydroxide was established.Pharmacodynamic effects were comprehensively assessed through behavioral scoring,histopathological analysis(hematoxylin and eosin)of nasal mucosa,and serum immunological markers(IgE,IL-4,and IFN-γ).The MAT-OMT in-situ gel showed no toxicity during ciliary movement in the toad palatal ciliary motility model.The MAT-OMT combination significantly alleviated nasal itching,sneezing,and rhinorrhea in guinea pigs with AR,mitigated mucosal edema and epithelial damage,and improved inflammatory cytokine levels,suggesting its efficacy in correcting Th1/Th2 immune imbalance.The comprehensive therapeutic effect of MAT-OMT was significantly superior to that of MAT or OMT alone and comparable to that of the positive control,budesonide.展开更多
AG(atrophic gastritis)is characterized by precancerous lesions associated with gastric cancer and can cause serious adverse health effects.The high incidence coupled with a low diagnosis rate and the mediocre effectiv...AG(atrophic gastritis)is characterized by precancerous lesions associated with gastric cancer and can cause serious adverse health effects.The high incidence coupled with a low diagnosis rate and the mediocre effectiveness of clinical treatment raises concerns.This article reviews the pathologic features,clinical manifestations,and treatment progress of AG.展开更多
Background:Allergic rhinitis(AR)is a kind of immune disease mediated by Ig E.We are intrigued by the potential role of DEK proto-oncogene(DEK)in inflammation-related diseases.We investigated the effects and mechanisms...Background:Allergic rhinitis(AR)is a kind of immune disease mediated by Ig E.We are intrigued by the potential role of DEK proto-oncogene(DEK)in inflammation-related diseases.We investigated the effects and mechanisms of DEK in treating AR,aiming to identify potential new treatment targets for AR.Methods:The AR mouse model was induced by house dust mite(HDM)(1 mg/m L).HNEp Cs stimulated by HDM(1 mg/m L)were pretreated for 24 h with or without DEK lentivirus.The effect of DEK knockout or knockdown on AR was evaluated in vitro and in vivo using western blotting,ELISA,flow cytometry,real-time quantitative PCR,immunohistochemistry,HE staining,PAS staining,Diff staining,and immunofluorescence.Results:After DEK knockdown,the inflammatory response of AR mice was reduced.In addition,DEK deletion mitigated nasal tissue damage and mitochondrial division.Our further studies showed that DEK deletion or inhibition led to the down-regulation of Rho A activity and decreased phosphorylation of Ezrin and Drp1 proteins,and inhibited mitochondrial division.Overall,DEK deficiency mitigated AR by down-regulating Rho A/Ezrin/Drp1 pathway activity.Conclusion:DEK alleviates AR through Rho A/Ezrin/Drp1 signaling pathway,which provides a new perspective for developing improved therapies and understanding the pathogenesis of AR.展开更多
Chronic atrophic gastritis(CAG)is a common type of chronic gastric disease characterized by high incidence and a certain tendency toward cancerization.In recent years,with the deepening of related research,it has been...Chronic atrophic gastritis(CAG)is a common type of chronic gastric disease characterized by high incidence and a certain tendency toward cancerization.In recent years,with the deepening of related research,it has been found that traditional Chinese medicine(TCM)exerts therapeutic effects on CAG through various signaling pathways.This article systematically reviews and summarizes the literature on TCM treatment of CAG,elaborating on the etiology and pathogenesis of CAG,new ideas from different physicians in treating CAG,and the effects of different TCM monomers and compound prescriptions on signaling pathways such as PI3K-AKT and JAK1/STAT3 to provide a reference for subsequent research on CAG.展开更多
OBJECTIVE:To explore the mechanism of Tonglaga-5(通拉嘎-5,TLG-5)for the treatment of chronic atrophic gastritis(CAG),based on network pharmacology and metabolomics.METHODS:Forty-eight male Sprague-Dawley rats were ran...OBJECTIVE:To explore the mechanism of Tonglaga-5(通拉嘎-5,TLG-5)for the treatment of chronic atrophic gastritis(CAG),based on network pharmacology and metabolomics.METHODS:Forty-eight male Sprague-Dawley rats were randomly divided into six groups(n=8):control group;model group;teprenone group,and low-,median-,and high-dose TLG-5 groups.The enzyme linked immunosorbent assay(ELISA)was used to measure the expression of pepsinogenⅠ(PGⅠ),pepsinogenⅡ(PGⅡ)and gastrin-17(G-17)in the serum.Hematoxylin and eosin staining were performed to observe the pathological condition.And the network pharmacology was employed to identify the targets and signaling pathways of TLG-5 affecting CAG.Then,the metabolomics approach was applied to explore the specific metabolites and metabolic pathways.Finally,validation was performed using the“metabolite-gene”interaction network,molecular docking and quantitative real-time polymerase chain reaction(q PCR).RESULTS:High-dose TLG-5 significantly improved the expression of PGⅠ,PGR(PGⅠ/PGⅡ)and G-17(P<0.05)and inhibited the expression of phosphoinositide-3-kinase regulatory subunit 2,AKT serine/threonine kinase(AKT),hypoxia-inducible factor 1-alpha(HIF-1α)(P<0.05).Further,high-dose TLG-5 reduced the number of glands was reduced,and fibrosis with oedema and ecchymosis appeared at the base.Overlapping TLG-5 and CAG gene targets produced 270 interactive targets.The results of gene ontology and Kyoto encyclopedia of genes and genomes enrichment analyses suggested that TLG-5 could affect CAG through the predominantly cancer and inflammation-related pathways.Pyrimidine metabolism was identified as a significantly differential pathway in the mechanism of TLG-5 for treating CAG.CONCLUSIONS:TLG-5 exerts a therapeutic effect on CAG by regulatingβ-alanine metabolism,pyrimidine metabolism pathways,and inhibiting the PI3K-AKT signaling pathway and HIF-1 signaling pathways.展开更多
Objective:To evaluate the therapeutic effects of Huangqi Sijun Decoction on chronic atrophic gastritis(CAG).Methods:Sixty CAG patients hospitalized between January 2020 and December 2022 were selected and randomly div...Objective:To evaluate the therapeutic effects of Huangqi Sijun Decoction on chronic atrophic gastritis(CAG).Methods:Sixty CAG patients hospitalized between January 2020 and December 2022 were selected and randomly divided into two groups using a random number table.The Traditional Chinese Medicine(TCM)group(n=30)was treated with Huangqi Sijun Decoction,while the Western medicine group(n=30)received omeprazole.The total effective rate,TCM syndrome scores,and serological indicators were compared.Results:The total effective rate in the TCM group was higher than that in the Western medicine group,while the adverse reaction rate was lower(P<0.05).Before treatment,there were no significant differences in TCM syndrome scores or serological indicators between the two groups(P>0.05).After treatment,the TCM group had lower TCM syndrome scores and better serological indicators compared to the Western medicine group(P<0.05).Conclusion:Huangqi Sijun Decoction can enhance the clinical efficacy of CAG patients,prevent adverse reactions,alleviate TCM symptoms,and regulate specific levels of serological indicators,demonstrating significant therapeutic advantages.展开更多
Traditional Chinese medicine(TCM)has been extensively explored with various naturally derived compounds as a potential therapeutic agent for chronic atrophic gastritis(CAG).In addition to the aspects discussed in the ...Traditional Chinese medicine(TCM)has been extensively explored with various naturally derived compounds as a potential therapeutic agent for chronic atrophic gastritis(CAG).In addition to the aspects discussed in the reviewed article,this invited commentary explores the initial available evidence on a fungus from TCM,Hericium erinaceus,in the context of CAG.Initial clinical data suggest the potential of this fungus in inducing clinical and histological improvements in patients with CAG,as well as a marked antimicrobial activity against Helicobacter pylori infection.Preclinical cellular evidence also indicates an antineoplastic role in gastric carcinogenesis,mediated by two components:Erinacine A and S.Further evidence is needed to propose this fungus as a potential complementary thera-peutic approach for CAG.展开更多
Objective:Although there is evidence to support the therapeutic effect of acupuncture on allergic rhinitis(AR),it is not clear whether acupuncture can achieve an efficacy comparable to that of pharmacological therapy....Objective:Although there is evidence to support the therapeutic effect of acupuncture on allergic rhinitis(AR),it is not clear whether acupuncture can achieve an efficacy comparable to that of pharmacological therapy.This multicenter,non-inferiority,single-blinded randomized controlled trial aimed to compare the differences in efficacy between warm needling(WN)therapy and loratadine(LO)in improving AR symptoms and quality of life.Methods:A total of 98 patients with persistent AR(PAR)were randomly divided into two groups in a 1:1 ratio:treatment group receiving WN therapy at Dazhui(GV14)and normal acupuncture at other acupoints for 12 sessions in 4 weeks and control group receiving LO at a dosage of 10 mg/day for oral administration.The study duration was 28 weeks(4-week treatment period with a 24-week follow-up).The primary outcome was the mean change in the Total Nasal Symptom Score(TNSS)from baseline to week 28,and the secondary outcomes included the Total Non-Nasal Symptom Score(TNNSS)and the Rhinoconjunctivitis Quality of Life Questionnaire(RQLQ)score.Statistical analyses were conducted us-ing SPSS22.0.Both intention-to-treat and treatment compliance analyses were performed to analyze all outcomes.Results:After intervention,TNSS scores improved from baseline to week 28 by 5.18(95%confidence interval[CI]:3.91,6.45;P<0.001)in the WN group and 4.59(95%CI 3.28,5.89;P<0.001)in the LO group,but the changes did not differ between the groups(P=0.515).The margin by 95%CI(−1.20,2.38)was not reached,indicating non-inferiority.WN therapy resulted in clinical improvements in TNNSS,with a significant difference com pared with LO treatment(f=5.466,P=0.021).WN therapy also resulted in clinical improvements in RQLQ scores,with a significant difference compared with LO(f=9.222,P=0.003).Conclusions:WN therapy demonstrated comparable efficacy to oral LO in improving nasal symptoms of PAR.However,it showed superior effects in alleviating concomitant nasal symptoms and enhancing patients’quality of life.展开更多
OBJECTIVE:To examine the effect of Xuanfei Tongqiao acupuncture on nasal inflammation in rats with allergic rhinitis(AR)and its underlying mechanisms,particularly focusing on the role of acupuncture in regulating the ...OBJECTIVE:To examine the effect of Xuanfei Tongqiao acupuncture on nasal inflammation in rats with allergic rhinitis(AR)and its underlying mechanisms,particularly focusing on the role of acupuncture in regulating the methylation of long non-coding RNA growth arrestspecific transcript 5(lnc RNA GAS5).METHODS:Interventions involved acupuncture or short hairpin RNA-GAS5(sh-GAS5)lentivirus in an ovalbumininduced AR rat model.Nasal mucosal tissues were collected post-intervention for pathological analysis.Parameters assessed included the proportion of type 2 innate lymphoid cells(ILC2s),the expression of GATA binding protein 3(GATA3),methyltransferase-like 3(METTL3),interleukin(IL)-33 proteins,and the expression of METTL3 and lnc RNA-GAS5.RESULTS:Xuanfei Tongqiao acupuncture and/or lentivirus sh-GAS5 significantly alleviated rhinitis symptoms and reduced nasal inflammation in AR rats.This was evidenced by a reduction in inflammatory cells,eosinophils,and cuprocytes in nasal mucosal tissues,along with a decreased proportion of ILC2s.Furthermore,protein levels of GATA3,METTL3,and IL-33,as well as the expression of METTL3 and lnc RNA-GAS5,were significantly reduced.These findings suggest that lnc RNA-GAS5 and its N6-methyladenosine methylation are key regulators of AR inflammation,with acupuncture exerting a modulatory effect on this pathway.CONCLUSIONS:Xuanfei Tongqiao acupuncture therapy inhibited the METTL3-GAS5-GATA3-IL-33-ILC2 pathway,reducing the inflammatory response and alleviating nasal symptoms in AR rats.These results suggest that Xuanfei Tongqiao acupuncture could serve as a potential alternative therapy for preventing and managing AR associated with inflammation.展开更多
Allergic rhinitis（AR）,with an increasing uptrend of the prevalence in many developed and developing countries,is a global health problem that affects people of all ages and ethnic groups.However,data on the prevalen...Allergic rhinitis（AR）,with an increasing uptrend of the prevalence in many developed and developing countries,is a global health problem that affects people of all ages and ethnic groups.However,data on the prevalence of self-reported AR in western China are rare.This study investigated the epidemiological features of self-reported AR in western China.In the cross-sectional,population-based study,a validated questionnaire survey on self-reported AR was carried out in 4 major cities in western China by multistage,stratified and cluster sampling,from January to December 2008.The total prevalence rate was 34.3%,with 32.3%（Chongqing）,34.3%（Chengdu）,37.9%（Urumqi）,30.3%（Nanning）,respectively.The prevalence presented to increase with age before 30 years old while decrease with age after 30 years old,and the highest prevalence was in 19-30 years group in Chongqing,Chengdu and Nanning which significantly showed ＂persistent and moderate-severe＂ type（P0.0001）;In Urumqi,there wasn＇t a significant increasing or decreasing trend of prevalence rate with age but with an ＂intermittent and mild＂predominance（P0.0001）.There were no distinct sexual differences in prevalence rates in the 4 cities.The morbidity was positively related to monthly average temperature and sunshine（r=0.76645,P=0.0036;r=0.67303,P=0.0165）,but negatively associated with relative humidity（r=-0.64391,P=0.0238） in Urumqi.Interestingly,the monthly morbidity was negatively associate with average temperature,sunshine and precipitation in Nanning（r=-0.81997,P=0.0011;r=-0.60787,P=0.0360;r=-0.59443,P=0.0415）.Self-reported AR is becoming common in western China with a rapid development in recent years,affecting about three persons out of ten.The climatic factors may have an indirect impact on the prevalence rate through the effects on the local allergens.展开更多
Current experimental models struggle to simulate the complex process of the transformation from atrophic gastritis to gastric cancer,while gastric organoid technology,especially region-specific modeling,provides a mor...Current experimental models struggle to simulate the complex process of the transformation from atrophic gastritis to gastric cancer,while gastric organoid technology,especially region-specific modeling,provides a more precise in vitro platform for studying this carcinogenic mechanism.Helicobacter pylori activates carcinogenic signaling pathways through virulence factors,inducing DNA damage,epigenetic dysregulation,and immune microenvironment imbalance,driving inflammation-cancer conversion.Intestinal metaplasia and spasmolytic polypeptide-expressing metaplasia serve as critical precursor lesions,gradually developing into dysplasia and adenocarcinoma under the influence of chronic inflammation and genetic instability through intestinal cell transformation and high trefoil factor 2-expressing cell expansion.The immune suppression,metabolic reprogramming,and matrix remodeling within the tumor microenvironment collaboratively create a pro-cancer ecosystem that accelerates inflammationcarcinogenesis transformation.The gastric organoid model successfully simulates the spatiotemporal dynamics of the carcinogenesis process in atrophic gastritis,and its future integration with single-cell omics,real-time imaging technologies,and artificial intelligence technologies could provide a more precise platform for elucidating molecular mechanisms and screening intervention strategies.These advances position gastric organoids as pivotal tools for clinical translation,enabling personalized risk stratification,early intervention targeting precancerous transitions,and ex vivo prediction of patient-specific therapeutic responses to guide precision management of gastric cancer.展开更多
OBJECTIVE:To evaluate the clinical efficacy and safety of Tuomin Zhiti decoction(脱敏止嚏汤,TZD)in the shortterm treatment of seasonal allergic rhinitis(SAR).METHODS:This study is a randomized,double-blind placebo-con...OBJECTIVE:To evaluate the clinical efficacy and safety of Tuomin Zhiti decoction(脱敏止嚏汤,TZD)in the shortterm treatment of seasonal allergic rhinitis(SAR).METHODS:This study is a randomized,double-blind placebo-controlled,and single-center clinical trial.In April 2021,during the spring pollen season in Beijing,94 SAR patients aged 19-60 years were randomized(1∶1 ratio)to receive two weeks of TZD or placebo.The primary outcomes were the change of Total Nasal Symptom Score(TNSS)and Total Ocular Symptom Score(TOSS)from baseline to the end of treatment.Secondary outcomes were the changed score of the mini Rhinoconjunctivitis Quality of Life Questionnaire(RQLQ),Rescue Medication Score(RMS),and Patient Global Impression of Change(PGIC).All adverse events were recorded and evaluated by two senior physicians.RESULTS:TZD significantly reduced both the total nasal symptom scores and total ocular symptom scores compared to scores at baseline,while the placebo group showed an increasing trajectory in both symptom scores.Compared to the placebo group,the TZD group also showed a greater improvement in the quality of life,scores of RMS,nasal and eye symptoms scores after TZD treatment(P<0.001).Most notably,at the end of treatment,the proportion of remission measured by PGIC was significantly higher in the TZD group(82.97%)compared with the placebo group(47.72%,P<0.001).CONCLUSION:This study suggested that two weeks of TZD is an effective and safe treatment for SAR patients and spring pollen allergy,TZD could significantly improve the nasal and eye symptoms and improve the quality of life of patients.展开更多
This study summarizes the clinical experience of professor LIAN Jianwei, a nationally renowned traditional Chinese medicine (TCM) practitioner, in treating chronic atrophic gastritis (CAG) with syndrome differentiatio...This study summarizes the clinical experience of professor LIAN Jianwei, a nationally renowned traditional Chinese medicine (TCM) practitioner, in treating chronic atrophic gastritis (CAG) with syndrome differentiation based on pulse-taking. Professor LIAN emphasizes the importance of pulse diagnosis in TCM clinical practice, focusing on the normal and variant aspects of pulse methods, and centers on the dynamic changes of the “Guan pulse” to accurately determine the functional status of the spleen and stomach based on pulse variations, thereby formulating personalized treatment plans. For patients with a slow and moderate pulse, spleen and stomach deficiency and dampness due to spleen deficiency are often identified, and treatment mainly focuses on strengthening the spleen, resolving dampness, and boosting Qi to aid transportation, usually with modifications of Shenling Baizhu San. When the left Guan pulse is string-like and the right Guan pulse is slow and moderate, it indicates liver depression and spleen deficiency, and Xiaoyao San is commonly used to soothe the liver, nourish the blood, and strengthen the spleen, harmonizing the liver and spleen. If the right Guan pulse is large and the left Guan pulse is string-like, it mostly belongs to liver and stomach Qi stagnation, and Bupleurum Liver-soothing powder is often used to soothe the liver, regulate Qi, and harmonize the stomach. A large and empty right Guan pulse suggests insufficiency of Central Qi, and Buzhong Yiqi Tang is commonly used to tonify Qi and soothe the liver. In terms of medication, professor LIAN pays attention to patients’ dietary habits and combines methods of eliminating dampness, promoting digestion, and activating blood circulation to improve symptoms and reverse atrophy.展开更多
OBJECTIVE:To identify the main active ingredients of Improved Yupingfeng Powder prescription(IYPFP,玉屏风散加味)and investigate its anti-inflammatory effects and underlying mechanisms in ovalbumins(OVA)-induced allerg...OBJECTIVE:To identify the main active ingredients of Improved Yupingfeng Powder prescription(IYPFP,玉屏风散加味)and investigate its anti-inflammatory effects and underlying mechanisms in ovalbumins(OVA)-induced allergic rhinitis(AR)in mice.METHODS:Bagg Albino/substrain mice were sensitized with OVA emulsified in aluminum hydroxide adjuvant,followed by intranasal challenge to establish AR models.Treatment groups received IYPFP(1.5 or 4.5 g/kg)via daily gavage for 14 d.The nasal mucosa tissues were collected for pathological observation.The expression of OVA-specific immunoglobulin E(Ig E),histamine,and interleukin-33(IL-33)was detected by enzyme-linked immunosorbent assay.IL-5,IL-13,IL-33,suppression of tumorigenicity 2(ST2),tumor necrosis factor-α(TNF-α)and interferon-γ(IFN-γ)in the nasal mucosa and lung were detected by quantitative polymerase chain reaction and Western blot.High performance liquid chromatography and ultra-high performance liquid chromatography quadrupole exactive orbitrap mass spectrometry were used to detect the chemical fingerprints of IYPFP,and the chemical compositions of plasma from rats treated with IYPFP at 0.5,1,1.5,2,3,4 and 6 h,respectively.Virtual screening of bioactive compounds was conducted through molecular docking targeting IL-33/ST2 pathway proteins.RESULTS:Eight chemical compounds of IYPFP were accurately identified,they are prim-O-glucosylcimifugin(peak 2),calycosin-7-O-β-D-glucoside(peak 4),cimifugin(peak 5),5-O-methylvisammioside(peak 6),sec-Oglucosylhamaudol(peak 12),calycosin(peak 15),formononetin(peak 19),and magnolin(peak 21).Compared with the OVA model group,IYPFP alleviated the nasal symptoms,improved nasal mucosal injury and downregulated the levels of OVA-specific Ig E,histamine and IL-33.Additionally,IYPFP reduced the levels of IL-5,IL-13,TNF-α,IL-33,and ST2 in the lungs,but upregulated IFN-γ.Molecular docking confirmed that eight representative compounds of IYPFP had good binding properties with IL-5,IL-13,IFN-γ,histamine,IL-33 and ST2,and were able to inhibit the activation of the IL33/ST2 inflammatory pathway.CONCLUSION:This study demonstrates that IYPFP ameliorates AR by modulating IL-33/ST2 pathway which provides a theoretical basis for the clinical treatment of patients with AR.展开更多
文摘Atrophic rhinitis is a disease which manifests itself mainly by anosmia due to dryness and atrophy of the nasal mucosa. There is no specific therapy for the disease at present. In the past few years, 23 cases of atrophic rhinitis were treated mainly by deep puncture at three points in the nasal region with satisfactory results. In order to find out the functional changes of the nasal mucosa, the mucociliary transport rate (MTR), surface temperature of the conchal mucosa, acid-base scale of nasal secretion, and volume of nasal secretion were determined before and after the treatment.
基金Supported by the National Natural Science Foundation of China,No.81860843Guangxi Administration of Traditional Chinese Medicine Project,No.GZSY23-36 and No.GXZYA20240150。
文摘BACKGROUND Chronic atrophic gastritis(CAG)is a clinically refractory gastric disease often characterized by high recurrence rates and adverse drug reactions.Anwei decoction(AWD),a traditional Chinese medicine formula,has been shown to significantly improve clinical symptoms in patients with CAG,as demonstrated by a multicenter cohort study(overall effective rate:82.5%,P<0.01).However,the unclear molecular mechanisms and therapeutic targets of AWD limit its international acceptance.AIM To investigate the therapeutic mechanisms of AWD against CAG from an integrated perspective.METHODS In this study,N-methyl-N’-nitro-N-nitrosoguanidine was used to establish a CAG rat model.Serum-derived constituents transferred from AWD were first identified using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry.The concentrations of inflammatory cytokines in serum samples were determined by enzyme-linked immunosorbent assay.Moreover,gastric mucosal tissues were analyzed by quantitative realtime polymerase chain reaction to measure messenger RNA(mRNA)levels of the NLRP3 inflammasome.Western blotting was used to detect the protein expression of NLRP3,caspase-1,and interleukin(IL)-1β.To elucidate the regulatory mechanisms underlying AWD treatment,structural alterations of the gut microbiota(GM)and associated metabolites were analyzed using integrated high-throughput sequencing(16S rRNA)and liquid chromatography-mass spectrometry based untargeted metabolomics.This comprehensive approach systematically clarified AWD’s multi-target therapeutic mechanisms against CAG.RESULTS AWD notably reduced serum levels of pro-inflammatory cytokines,such as IL-1β,IL-18,tumor necrosis factor-α,and lipopolysaccharide,demonstrating significant statistical differences(all P<0.01).Additionally,AWD substantially inhibited NLRP3 mRNA expression in gastric mucosal tissue(P<0.01)and concurrently decreased the protein abundance of NLRP3,IL-1β,and caspase-1(all P<0.01),thereby suppressing inflammasome signaling activation.GM analysis indicated that AWD intervention significantly increased the relative abundance of beneficial bacteria.Associated microbial metabolites likely inhibited the NLRP3 inflammasome pathway by modulating immune cell function.Non-targeted metabolomics further indicated that AWD exerted anti-inflammatory effects by regulating critical metabolic pathways,including the Kaposi’s sarcoma-associated herpesvirus infection pathway,autophagy processes,and glycosylphosphatidylinositol-anchor biosynthesis.CONCLUSION AWD alleviates the pathological progression of CAG through multi-target synergistic mechanisms.On one hand,AWD directly suppresses gastric mucosal inflammation by inhibiting NLRP3 inflammasome activation.On the other hand,AWD remodels intestinal microbiota-metabolite homeostasis,enhances intestinal barrier function,and regulates mucosal immune responses.
基金supported by the National Natural Science Foundation of China(Grant No.82274442)the Key Research Project in Traditional Chinese Medicine of Tianjin Municipal Health Commission(Grant No.202007)the Integrated Traditional Chinese and Western Medicine Research Project of Tianjin Municipal Health Commission(Grant No.2023134).
文摘Background:The development of gastric cancer(GC)encompasses precancerous conditions like chronic atrophic gastritis(CAG)and premalignant lesions of gastric cancer(PLGC).In these situations,abnormal Notch signaling results in mucosal impairment and the initiation of cancer.Banxia Xiexin Decoction(BXD),a well-known formula in traditional Chinese medicine(TCM),shows promise in treating gastric disorders,but its mechanisms in gastric restoration remain unclear.Methods:Using MNNG-induced CAG and PLGC rat models,BXD was administered for 12 weeks.Gastric mucosal pathology was assessed via hematoxylin-eosin staining.Proliferation(Ki-67)and angiogenesis(VEGFA)markers were evaluated by immunohistochemistry.Network pharmacology identified BXD’s targets and pathways.Notch pathway components(Notch1,Jagged1,Dll4,Hes1)were analyzed via qPCR,Western blot,and immunohistochemistry.Results:BXD significantly ameliorated mucosal atrophy,glandular structural disorder,and dysplasia in CAG and PLGC rats.Network pharmacology revealed 323 overlapping targets between BXD and PLGC,with Notch signaling as a central pathway.BXD downregulated Notch1,Jagged1,Dll4,and Hes1 expression at transcriptional and protein levels,suppressed Ki-67(proliferation)and VEGFA(angiogenesis)overexpression,and restored gastric mucosal integrity.Conclusion:BXD inhibits Notch signaling,reduces aberrant proliferation and angiogenesis,and interrupts Correa’s gastric carcinogenesis cascade.This study provides mechanistic evidence supporting BXD as a TCM-based intervention for gastric precancerous lesions.
基金Supported by General Project of Shanxi Administration of Traditional Chinese Medicine(2023ZYYA007&2024ZYY2D009)Young Scientists Project of Shanxi Provincial Department of Science and Technology(202203021212026)Scientific and Technological Innovation Capacity Cultivation Program for Basic and Clinical Cooperative Research Project of Shanxi University of Chinese Medicine(2024PY-JL-23-01).
文摘This study makes an in-depth exploration of the core pathogenesis of chronic atrophic gastritis,Qi Deficiency with Stagnation,to systematically interpret it within the theoretical framework of Achieving Central Harmony,and to provide a treatment plan.Professor Li Tingquan believes that the occurrence and development of chronic atrophic gastritis(CAG)is fundamentally a process of harmony imbalance of the spleen and stomach.Its specific manifestation lies in the interaction between qi deficiency and stagnation,namely,spleen deficiency as the root cause,and qi stagnation,phlegm-dampness,and blood stasis obstructing the middle energizer as the secondary manifestations.These factors are mutually causal and interact with one another.Based on this,he proposed a four-step method to treat the disease:eliminating pathogenic factors,harmonizing the spleen and stomach,activating blood circulation to resolve stasis,and tonifying the kidney to generate blood(metaphorically described as"closing the mountains to cultivate forests and thicken the soil,breeding seeds and irrigating for stomach recovery").This approach aims to restore the harmonious state of the middle energizer,providing a complete clinical framework of theory,principle,formula,and herbs for the prevention and treatment of CAG.
基金Supported by the Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences,No.CI2021A00806High Level Chinese Medical Hospital Promotion Project,No.HLCMHPP2023086the Fundamental Research Funds for the Central Public Welfare Research Institutes,No.ZZ17-XRZ-041.
文摘Chronic atrophic gastritis(CAG)is an important stage of precancerous lesions of gastric cancer.Effective treatment and regulation of CAG are essential to prevent its progression to malignancy.Traditional Chinese medicine(TCM)has shown multi-targeted efficacy in CAG treatment,with advantages in enhancing gastric mucosal barrier defense,improving microcirculation,modulating inflammatory and immune responses,and promoting lesion healing,etc.Clinical studies and meta-analyses indicate that TCM provides significant benefits,with specific Chinese herbal compounds and monomers demonstrating protective effects on the gastric mucosa through mechanisms including anti-inflammation,antioxidation,and regulation of cellular proliferation and apoptosis,etc.Finally,it is pointed out that the efficacy of TCM in the treatment of CAG requires standardized research and unified standards,and constantly clarifies and improves the evaluation criteria of each dimension of gastric mucosal barrier function.
基金the National Natural Science Foundation of China:to Explore the Mechanism of Acupuncture Treatment on Rats with Allergic Rhinitis from the Hypothalamic-Pituitary-Adrenal (HPA) Axis and Serum Immune Factor Levels (No.82004455)。
文摘OBJECTIVE:To explore the correlation between acupuncture treatment for allergic rhinitis(AR) and hypothalamic-pituitary-adrenal(HPA) axis regulation by investigating changes in serum immune factors,HPA axis-associated hormone levels,activation levels of paraventricular nucleus(PVN) neurons,and the severity of nasal mucosal lesions,in rats with AR before and after acupuncture treatment.METHODS:After establishing the AR rat model,ovalbumin was administered via nasal drip to all groups except the blank control.Each group received continuous treatment for 14 d:the acupuncture,acupuncture + RU-486(mifepristone),and RU-486 groups received acupuncture only,RU486 intraperitoneal injection and acupuncture,and RU-486 intraperitoneal injection only,respectively.Following the intervention period,behavioral scoring was performed on all AR rats,and peripheral blood,nasal mucosa samples,and brains tissue(containing PVN region) were obtained following euthanization.Interleukin(IL-4,IL-5,IL-13),interferon gamma(IFN-γ),corticosterone(CORT),and corticotrophin-releasing hormone(CRH) levels were evaluated in peripheral blood samples.Adrenocorticotropic hormone(ACTH) levels were determined using an enzyme-linked immunoassay assay,and hematoxylin and eosin staining was performed on the nasal mucosa samples.The expression levels of c-Fos in PVN neurons following acupuncture treatment were evaluated by immunofluorescent staining.RESULTS:Following the intervention period,the behavioral scores for the blank control group were lower than those of other groups(P < 0.05),while the acupuncture group scores were lower than those in the model control,acupuncture + RU486,and RU486 groups(P < 0.05).The blank control group had lower serum IL-4,IL-5,and IL-13 levels than those in the other groups(P < 0.05).The acupuncture group had lower serum IL-4,IL-5,and IL-13 levels than those in the model control,acupuncture + RU486,and RU486 groups(P < 0.05).The blank control group had the highest serum IFN-γ levels among all groups,followed by the acupuncture group.The serum CORT,CRH,and ACTH levels in the blank control group were lower than those in the remaining groups(P < 0.05).These biomarker levels were also lower in the acupuncture group than those in the model control,acupuncture + RU486,and RU486 groups(P < 0.05).Compared with model,rats in the acupuncture group exhibited an increased c-Fos expression in PVN neurons.CONCLUSION:Acupuncture can alleviate AR symptoms and regulate serum inflammatory factor levels and HPA axis-related hormones in AR rats.Moreover,these effects are inhibited by glucocorticoid antagonists,suggesting that acupuncture may regulate AR symptoms through HPA axis regulation.
基金supported by the National Key Research and Development Program of China(No.2024YFE0111300).
文摘Sophorae Flavescentis Radix(Ku Shen)is a traditional Chinese medicine used to treat damp-heat syndrome-related diseases,such as dysentery,jaundice,rhinitis,and skin inflammation.Recent studies have revealed the potential pharmacological value of its active alkaloid components,matrine(MAT)and oxymatrine(OMT),in the treatment of allergic rhinitis(AR).To develop a thermosensitive in-situ gel containing MAT and OMT for the intranasal treatment of AR,a thermosensitive matrix composed of Pluronic F127/F68 was optimized using the Box-Behnken Response Surface Methodology.The biosafety of the formulation was evaluated using a palatal ciliary movement model.A guinea pig model of AR induced by ovalbumin and aluminum hydroxide was established.Pharmacodynamic effects were comprehensively assessed through behavioral scoring,histopathological analysis(hematoxylin and eosin)of nasal mucosa,and serum immunological markers(IgE,IL-4,and IFN-γ).The MAT-OMT in-situ gel showed no toxicity during ciliary movement in the toad palatal ciliary motility model.The MAT-OMT combination significantly alleviated nasal itching,sneezing,and rhinorrhea in guinea pigs with AR,mitigated mucosal edema and epithelial damage,and improved inflammatory cytokine levels,suggesting its efficacy in correcting Th1/Th2 immune imbalance.The comprehensive therapeutic effect of MAT-OMT was significantly superior to that of MAT or OMT alone and comparable to that of the positive control,budesonide.
文摘AG(atrophic gastritis)is characterized by precancerous lesions associated with gastric cancer and can cause serious adverse health effects.The high incidence coupled with a low diagnosis rate and the mediocre effectiveness of clinical treatment raises concerns.This article reviews the pathologic features,clinical manifestations,and treatment progress of AG.
基金National Natural Science Foundation of China,Grant/Award Number:82260007 and 82260218The Department of Education of Jilin Province,(JJKH20240698KJ)+1 种基金Natural Science Research Foundation of Jilin Province for Sciences and Technology,Grant/Award Number:20210101215JC20240602100RC and 20240404025ZP。
文摘Background:Allergic rhinitis(AR)is a kind of immune disease mediated by Ig E.We are intrigued by the potential role of DEK proto-oncogene(DEK)in inflammation-related diseases.We investigated the effects and mechanisms of DEK in treating AR,aiming to identify potential new treatment targets for AR.Methods:The AR mouse model was induced by house dust mite(HDM)(1 mg/m L).HNEp Cs stimulated by HDM(1 mg/m L)were pretreated for 24 h with or without DEK lentivirus.The effect of DEK knockout or knockdown on AR was evaluated in vitro and in vivo using western blotting,ELISA,flow cytometry,real-time quantitative PCR,immunohistochemistry,HE staining,PAS staining,Diff staining,and immunofluorescence.Results:After DEK knockdown,the inflammatory response of AR mice was reduced.In addition,DEK deletion mitigated nasal tissue damage and mitochondrial division.Our further studies showed that DEK deletion or inhibition led to the down-regulation of Rho A activity and decreased phosphorylation of Ezrin and Drp1 proteins,and inhibited mitochondrial division.Overall,DEK deficiency mitigated AR by down-regulating Rho A/Ezrin/Drp1 pathway activity.Conclusion:DEK alleviates AR through Rho A/Ezrin/Drp1 signaling pathway,which provides a new perspective for developing improved therapies and understanding the pathogenesis of AR.
基金supported by the Youth Medical Science and Technology Talent Special Research Project of Xinjiang Uygur Autonomous Region Health Commission(WJWY-202440).
文摘Chronic atrophic gastritis(CAG)is a common type of chronic gastric disease characterized by high incidence and a certain tendency toward cancerization.In recent years,with the deepening of related research,it has been found that traditional Chinese medicine(TCM)exerts therapeutic effects on CAG through various signaling pathways.This article systematically reviews and summarizes the literature on TCM treatment of CAG,elaborating on the etiology and pathogenesis of CAG,new ideas from different physicians in treating CAG,and the effects of different TCM monomers and compound prescriptions on signaling pathways such as PI3K-AKT and JAK1/STAT3 to provide a reference for subsequent research on CAG.
基金the National Natural Science Foundation of China:New Quality Control Model of Mongolian Medicine Tonglaga-5“Qingzhuo Shenghua”Based on“Stomach Blood Bone Correlation Tracking”(No.82160745)Grassland Talent Rolling Support Program([2023]3)+1 种基金Key Projects of Inner Mongolia Medical University:Research on the Mechanism of Mongolian Prescription Tonglaga-5 in Treating Chronic Atrophic Gastritis based on Multiomics Integration(No.YKD2024ZD001)Scientific Research Projects of the Inner Mongolia Autonomous Region Collaborative Innovation Centre of Mongolian Medicine:Preparation and Pharmacological Study of Mongolian Medicine Tonglaga-5 Transdermal Patch(No.MYYXTPY202313)。
文摘OBJECTIVE:To explore the mechanism of Tonglaga-5(通拉嘎-5,TLG-5)for the treatment of chronic atrophic gastritis(CAG),based on network pharmacology and metabolomics.METHODS:Forty-eight male Sprague-Dawley rats were randomly divided into six groups(n=8):control group;model group;teprenone group,and low-,median-,and high-dose TLG-5 groups.The enzyme linked immunosorbent assay(ELISA)was used to measure the expression of pepsinogenⅠ(PGⅠ),pepsinogenⅡ(PGⅡ)and gastrin-17(G-17)in the serum.Hematoxylin and eosin staining were performed to observe the pathological condition.And the network pharmacology was employed to identify the targets and signaling pathways of TLG-5 affecting CAG.Then,the metabolomics approach was applied to explore the specific metabolites and metabolic pathways.Finally,validation was performed using the“metabolite-gene”interaction network,molecular docking and quantitative real-time polymerase chain reaction(q PCR).RESULTS:High-dose TLG-5 significantly improved the expression of PGⅠ,PGR(PGⅠ/PGⅡ)and G-17(P<0.05)and inhibited the expression of phosphoinositide-3-kinase regulatory subunit 2,AKT serine/threonine kinase(AKT),hypoxia-inducible factor 1-alpha(HIF-1α)(P<0.05).Further,high-dose TLG-5 reduced the number of glands was reduced,and fibrosis with oedema and ecchymosis appeared at the base.Overlapping TLG-5 and CAG gene targets produced 270 interactive targets.The results of gene ontology and Kyoto encyclopedia of genes and genomes enrichment analyses suggested that TLG-5 could affect CAG through the predominantly cancer and inflammation-related pathways.Pyrimidine metabolism was identified as a significantly differential pathway in the mechanism of TLG-5 for treating CAG.CONCLUSIONS:TLG-5 exerts a therapeutic effect on CAG by regulatingβ-alanine metabolism,pyrimidine metabolism pathways,and inhibiting the PI3K-AKT signaling pathway and HIF-1 signaling pathways.
基金Inner Mongolia Autonomous Region Education Science“14th Five-Year Plan”Project(Project No.NGJGH2023467)Inner Mongolia Medical University Higher Education Teaching Reform Research Project(Project No.NYJXGG2022054)。
文摘Objective:To evaluate the therapeutic effects of Huangqi Sijun Decoction on chronic atrophic gastritis(CAG).Methods:Sixty CAG patients hospitalized between January 2020 and December 2022 were selected and randomly divided into two groups using a random number table.The Traditional Chinese Medicine(TCM)group(n=30)was treated with Huangqi Sijun Decoction,while the Western medicine group(n=30)received omeprazole.The total effective rate,TCM syndrome scores,and serological indicators were compared.Results:The total effective rate in the TCM group was higher than that in the Western medicine group,while the adverse reaction rate was lower(P<0.05).Before treatment,there were no significant differences in TCM syndrome scores or serological indicators between the two groups(P>0.05).After treatment,the TCM group had lower TCM syndrome scores and better serological indicators compared to the Western medicine group(P<0.05).Conclusion:Huangqi Sijun Decoction can enhance the clinical efficacy of CAG patients,prevent adverse reactions,alleviate TCM symptoms,and regulate specific levels of serological indicators,demonstrating significant therapeutic advantages.
文摘Traditional Chinese medicine(TCM)has been extensively explored with various naturally derived compounds as a potential therapeutic agent for chronic atrophic gastritis(CAG).In addition to the aspects discussed in the reviewed article,this invited commentary explores the initial available evidence on a fungus from TCM,Hericium erinaceus,in the context of CAG.Initial clinical data suggest the potential of this fungus in inducing clinical and histological improvements in patients with CAG,as well as a marked antimicrobial activity against Helicobacter pylori infection.Preclinical cellular evidence also indicates an antineoplastic role in gastric carcinogenesis,mediated by two components:Erinacine A and S.Further evidence is needed to propose this fungus as a potential complementary thera-peutic approach for CAG.
基金Supported by Beijing Municipal Science&Technology Commission Program:Z151100004015117Shenzhen Science and Technology Plan Project:JCYJ20210324120804012Shenzhen Luohu District Soft Science Research Plan project:LX202202128。
文摘Objective:Although there is evidence to support the therapeutic effect of acupuncture on allergic rhinitis(AR),it is not clear whether acupuncture can achieve an efficacy comparable to that of pharmacological therapy.This multicenter,non-inferiority,single-blinded randomized controlled trial aimed to compare the differences in efficacy between warm needling(WN)therapy and loratadine(LO)in improving AR symptoms and quality of life.Methods:A total of 98 patients with persistent AR(PAR)were randomly divided into two groups in a 1:1 ratio:treatment group receiving WN therapy at Dazhui(GV14)and normal acupuncture at other acupoints for 12 sessions in 4 weeks and control group receiving LO at a dosage of 10 mg/day for oral administration.The study duration was 28 weeks(4-week treatment period with a 24-week follow-up).The primary outcome was the mean change in the Total Nasal Symptom Score(TNSS)from baseline to week 28,and the secondary outcomes included the Total Non-Nasal Symptom Score(TNNSS)and the Rhinoconjunctivitis Quality of Life Questionnaire(RQLQ)score.Statistical analyses were conducted us-ing SPSS22.0.Both intention-to-treat and treatment compliance analyses were performed to analyze all outcomes.Results:After intervention,TNSS scores improved from baseline to week 28 by 5.18(95%confidence interval[CI]:3.91,6.45;P<0.001)in the WN group and 4.59(95%CI 3.28,5.89;P<0.001)in the LO group,but the changes did not differ between the groups(P=0.515).The margin by 95%CI(−1.20,2.38)was not reached,indicating non-inferiority.WN therapy resulted in clinical improvements in TNNSS,with a significant difference com pared with LO treatment(f=5.466,P=0.021).WN therapy also resulted in clinical improvements in RQLQ scores,with a significant difference compared with LO(f=9.222,P=0.003).Conclusions:WN therapy demonstrated comparable efficacy to oral LO in improving nasal symptoms of PAR.However,it showed superior effects in alleviating concomitant nasal symptoms and enhancing patients’quality of life.
基金Open Project of National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion:Multi-Center Clinical Study on Xuanfei Tongqiao Acupuncture combined with Sphenopalatine Ganglion Stimulation in the Treatment of Allergic Rhinitis(No.NCRCOP2023008)Foundation of First Teaching Hospital of Tianjin University of Traditional Chinese Medicine:Clinical Observation on Preventive Acupuncture for Seasonal Allergic Rhinitis based on the Theory of"Strong Spleen Prevents Pathogenic Invasion"(No.2020043)the Medical Research Project of Yancheng City Health Commission:Mechanism of Xuanfei Tongqiao Acupuncture in Treating Allergic Rhinitis via m6A Methylation Modification of lncRNA GAS5(No.YK2023017)。
文摘OBJECTIVE:To examine the effect of Xuanfei Tongqiao acupuncture on nasal inflammation in rats with allergic rhinitis(AR)and its underlying mechanisms,particularly focusing on the role of acupuncture in regulating the methylation of long non-coding RNA growth arrestspecific transcript 5(lnc RNA GAS5).METHODS:Interventions involved acupuncture or short hairpin RNA-GAS5(sh-GAS5)lentivirus in an ovalbumininduced AR rat model.Nasal mucosal tissues were collected post-intervention for pathological analysis.Parameters assessed included the proportion of type 2 innate lymphoid cells(ILC2s),the expression of GATA binding protein 3(GATA3),methyltransferase-like 3(METTL3),interleukin(IL)-33 proteins,and the expression of METTL3 and lnc RNA-GAS5.RESULTS:Xuanfei Tongqiao acupuncture and/or lentivirus sh-GAS5 significantly alleviated rhinitis symptoms and reduced nasal inflammation in AR rats.This was evidenced by a reduction in inflammatory cells,eosinophils,and cuprocytes in nasal mucosal tissues,along with a decreased proportion of ILC2s.Furthermore,protein levels of GATA3,METTL3,and IL-33,as well as the expression of METTL3 and lnc RNA-GAS5,were significantly reduced.These findings suggest that lnc RNA-GAS5 and its N6-methyladenosine methylation are key regulators of AR inflammation,with acupuncture exerting a modulatory effect on this pathway.CONCLUSIONS:Xuanfei Tongqiao acupuncture therapy inhibited the METTL3-GAS5-GATA3-IL-33-ILC2 pathway,reducing the inflammatory response and alleviating nasal symptoms in AR rats.These results suggest that Xuanfei Tongqiao acupuncture could serve as a potential alternative therapy for preventing and managing AR associated with inflammation.
基金supported by the Science Research grants for the 10th 5-year plan to SL.H. (2004BA720A19-02)
文摘Allergic rhinitis（AR）,with an increasing uptrend of the prevalence in many developed and developing countries,is a global health problem that affects people of all ages and ethnic groups.However,data on the prevalence of self-reported AR in western China are rare.This study investigated the epidemiological features of self-reported AR in western China.In the cross-sectional,population-based study,a validated questionnaire survey on self-reported AR was carried out in 4 major cities in western China by multistage,stratified and cluster sampling,from January to December 2008.The total prevalence rate was 34.3%,with 32.3%（Chongqing）,34.3%（Chengdu）,37.9%（Urumqi）,30.3%（Nanning）,respectively.The prevalence presented to increase with age before 30 years old while decrease with age after 30 years old,and the highest prevalence was in 19-30 years group in Chongqing,Chengdu and Nanning which significantly showed ＂persistent and moderate-severe＂ type（P0.0001）;In Urumqi,there wasn＇t a significant increasing or decreasing trend of prevalence rate with age but with an ＂intermittent and mild＂predominance（P0.0001）.There were no distinct sexual differences in prevalence rates in the 4 cities.The morbidity was positively related to monthly average temperature and sunshine（r=0.76645,P=0.0036;r=0.67303,P=0.0165）,but negatively associated with relative humidity（r=-0.64391,P=0.0238） in Urumqi.Interestingly,the monthly morbidity was negatively associate with average temperature,sunshine and precipitation in Nanning（r=-0.81997,P=0.0011;r=-0.60787,P=0.0360;r=-0.59443,P=0.0415）.Self-reported AR is becoming common in western China with a rapid development in recent years,affecting about three persons out of ten.The climatic factors may have an indirect impact on the prevalence rate through the effects on the local allergens.
基金Supported by National Traditional Chinese Medicine Advantageous Specialty Project of National Administration of Traditional Chinese MedicineShuguang Hospital Siming Foundation Research Special Project,No.SGKJ-202304.
文摘Current experimental models struggle to simulate the complex process of the transformation from atrophic gastritis to gastric cancer,while gastric organoid technology,especially region-specific modeling,provides a more precise in vitro platform for studying this carcinogenic mechanism.Helicobacter pylori activates carcinogenic signaling pathways through virulence factors,inducing DNA damage,epigenetic dysregulation,and immune microenvironment imbalance,driving inflammation-cancer conversion.Intestinal metaplasia and spasmolytic polypeptide-expressing metaplasia serve as critical precursor lesions,gradually developing into dysplasia and adenocarcinoma under the influence of chronic inflammation and genetic instability through intestinal cell transformation and high trefoil factor 2-expressing cell expansion.The immune suppression,metabolic reprogramming,and matrix remodeling within the tumor microenvironment collaboratively create a pro-cancer ecosystem that accelerates inflammationcarcinogenesis transformation.The gastric organoid model successfully simulates the spatiotemporal dynamics of the carcinogenesis process in atrophic gastritis,and its future integration with single-cell omics,real-time imaging technologies,and artificial intelligence technologies could provide a more precise platform for elucidating molecular mechanisms and screening intervention strategies.These advances position gastric organoids as pivotal tools for clinical translation,enabling personalized risk stratification,early intervention targeting precancerous transitions,and ex vivo prediction of patient-specific therapeutic responses to guide precision management of gastric cancer.
基金Supported by the Fundamental Research Funds for the Central Universities(No.2023-JYB-JBZD-009)High level Key Discipline of National Administration of Traditional Chinese Medicine-Traditional Chinese Constitutional Medicine(No.zyyzdxk-2023251)+1 种基金General program of National Natural Science Foundation of China:Study on the Mechanism of"the Simultaneous Prevention of Different Diseases"of Allergic Constitution Regulating Formula based on DNA Trap Mediated Eosinophil-Derived Dendritic Cells Cell Crosstalk(No.82174243)General Project of Beijing Natural Science Foundation:Study on the Mechanism of Guominkang for Treating Allergic Rhinitis through Regulating Body Constitution based on Vacuolating Cytotoxin A-mediated Eosinophil-derived Dendritic Cell Cell immune microenvironment(No.7242227)。
文摘OBJECTIVE:To evaluate the clinical efficacy and safety of Tuomin Zhiti decoction(脱敏止嚏汤,TZD)in the shortterm treatment of seasonal allergic rhinitis(SAR).METHODS:This study is a randomized,double-blind placebo-controlled,and single-center clinical trial.In April 2021,during the spring pollen season in Beijing,94 SAR patients aged 19-60 years were randomized(1∶1 ratio)to receive two weeks of TZD or placebo.The primary outcomes were the change of Total Nasal Symptom Score(TNSS)and Total Ocular Symptom Score(TOSS)from baseline to the end of treatment.Secondary outcomes were the changed score of the mini Rhinoconjunctivitis Quality of Life Questionnaire(RQLQ),Rescue Medication Score(RMS),and Patient Global Impression of Change(PGIC).All adverse events were recorded and evaluated by two senior physicians.RESULTS:TZD significantly reduced both the total nasal symptom scores and total ocular symptom scores compared to scores at baseline,while the placebo group showed an increasing trajectory in both symptom scores.Compared to the placebo group,the TZD group also showed a greater improvement in the quality of life,scores of RMS,nasal and eye symptoms scores after TZD treatment(P<0.001).Most notably,at the end of treatment,the proportion of remission measured by PGIC was significantly higher in the TZD group(82.97%)compared with the placebo group(47.72%,P<0.001).CONCLUSION:This study suggested that two weeks of TZD is an effective and safe treatment for SAR patients and spring pollen allergy,TZD could significantly improve the nasal and eye symptoms and improve the quality of life of patients.
基金supported by the Zhejiang Science and Technology Program of TCM(No.2025ZF017)the Clinical Young-Talent Trainning Project in TCM under the Young Eagle Program of the China Association of Chinese Medicine(No.CYJH2024048).
文摘This study summarizes the clinical experience of professor LIAN Jianwei, a nationally renowned traditional Chinese medicine (TCM) practitioner, in treating chronic atrophic gastritis (CAG) with syndrome differentiation based on pulse-taking. Professor LIAN emphasizes the importance of pulse diagnosis in TCM clinical practice, focusing on the normal and variant aspects of pulse methods, and centers on the dynamic changes of the “Guan pulse” to accurately determine the functional status of the spleen and stomach based on pulse variations, thereby formulating personalized treatment plans. For patients with a slow and moderate pulse, spleen and stomach deficiency and dampness due to spleen deficiency are often identified, and treatment mainly focuses on strengthening the spleen, resolving dampness, and boosting Qi to aid transportation, usually with modifications of Shenling Baizhu San. When the left Guan pulse is string-like and the right Guan pulse is slow and moderate, it indicates liver depression and spleen deficiency, and Xiaoyao San is commonly used to soothe the liver, nourish the blood, and strengthen the spleen, harmonizing the liver and spleen. If the right Guan pulse is large and the left Guan pulse is string-like, it mostly belongs to liver and stomach Qi stagnation, and Bupleurum Liver-soothing powder is often used to soothe the liver, regulate Qi, and harmonize the stomach. A large and empty right Guan pulse suggests insufficiency of Central Qi, and Buzhong Yiqi Tang is commonly used to tonify Qi and soothe the liver. In terms of medication, professor LIAN pays attention to patients’ dietary habits and combines methods of eliminating dampness, promoting digestion, and activating blood circulation to improve symptoms and reverse atrophy.
基金National Natural Science Foundation of China:Exploring the Mechanism of Improved Yupingfeng Powder in Treating Allergic Rhinitis Based on the Nucleotide-Binding Oligomerization Domain,Leucine-rich Repeat and Pyrin Domain-containing Protein 3/Interleukin-33-Mediated Activation Pathway of Pulmonary Group 2 Innate Lymphoid Cells in Airway Epithelial Cells(No.82374526)Science and Technology Project of Guangzhou:Mechanism of Shikonin in Treating Acute Lung Injury Through Regulating M1 Macrophage Polarization via Adenosine 5'-monophosphate-Activated Protein Kinase/Dynamin-Related Protein 1-mediated Mitochondrial Dynamics(No.2024A04J4334)+2 种基金Exemplary Study on Process Optimization and Quality Standard Enhancement of Hospital Preparations Such as Gangmei Qingyan Mixture(No.2023A03J0299)Anti-Allergic Mechanism of Improved Yupingfeng Powder in Alleviating Nasal Mucosal Inflammation in Allergic Rhinitis via Interleukin-33/Suppression of Tumorigenicity 2-Regulated Group 2 Innate Lymphoid Cells Suppression(No.202201020457)Elite Talent Program of the First Affiliated Hospital of Guangzhou University of Chinese Medicine by 2023 Years and Guangdong Province Lingnan Characteristic Hospital Preparation Transformation Engineering Technology Research Center(No.2023A170)。
文摘OBJECTIVE:To identify the main active ingredients of Improved Yupingfeng Powder prescription(IYPFP,玉屏风散加味)and investigate its anti-inflammatory effects and underlying mechanisms in ovalbumins(OVA)-induced allergic rhinitis(AR)in mice.METHODS:Bagg Albino/substrain mice were sensitized with OVA emulsified in aluminum hydroxide adjuvant,followed by intranasal challenge to establish AR models.Treatment groups received IYPFP(1.5 or 4.5 g/kg)via daily gavage for 14 d.The nasal mucosa tissues were collected for pathological observation.The expression of OVA-specific immunoglobulin E(Ig E),histamine,and interleukin-33(IL-33)was detected by enzyme-linked immunosorbent assay.IL-5,IL-13,IL-33,suppression of tumorigenicity 2(ST2),tumor necrosis factor-α(TNF-α)and interferon-γ(IFN-γ)in the nasal mucosa and lung were detected by quantitative polymerase chain reaction and Western blot.High performance liquid chromatography and ultra-high performance liquid chromatography quadrupole exactive orbitrap mass spectrometry were used to detect the chemical fingerprints of IYPFP,and the chemical compositions of plasma from rats treated with IYPFP at 0.5,1,1.5,2,3,4 and 6 h,respectively.Virtual screening of bioactive compounds was conducted through molecular docking targeting IL-33/ST2 pathway proteins.RESULTS:Eight chemical compounds of IYPFP were accurately identified,they are prim-O-glucosylcimifugin(peak 2),calycosin-7-O-β-D-glucoside(peak 4),cimifugin(peak 5),5-O-methylvisammioside(peak 6),sec-Oglucosylhamaudol(peak 12),calycosin(peak 15),formononetin(peak 19),and magnolin(peak 21).Compared with the OVA model group,IYPFP alleviated the nasal symptoms,improved nasal mucosal injury and downregulated the levels of OVA-specific Ig E,histamine and IL-33.Additionally,IYPFP reduced the levels of IL-5,IL-13,TNF-α,IL-33,and ST2 in the lungs,but upregulated IFN-γ.Molecular docking confirmed that eight representative compounds of IYPFP had good binding properties with IL-5,IL-13,IFN-γ,histamine,IL-33 and ST2,and were able to inhibit the activation of the IL33/ST2 inflammatory pathway.CONCLUSION:This study demonstrates that IYPFP ameliorates AR by modulating IL-33/ST2 pathway which provides a theoretical basis for the clinical treatment of patients with AR.
