目的:探讨昼夜节律因子基因——核受体亚家族1 D组成员1和2(nuclear receptor subfamily 1group D members 1 and 2,NR1D1和NR1D2;分别编码REV-ERBα和REV-ERBβ蛋白)在自然衰老小鼠血管中的表达变化及对血管重塑的影响。方法:(1)将6月...目的:探讨昼夜节律因子基因——核受体亚家族1 D组成员1和2(nuclear receptor subfamily 1group D members 1 and 2,NR1D1和NR1D2;分别编码REV-ERBα和REV-ERBβ蛋白)在自然衰老小鼠血管中的表达变化及对血管重塑的影响。方法:(1)将6月龄小鼠设为青年组,24月龄小鼠设为老年组,每组各6只。称取腓肠肌、脾和胸腺重量并计算相应指数;苏木精-伊红、Masson、油红O、衰老相关β-半乳糖苷酶(senescence-associatedβ-galactosidase,SA-β-Gal)和二氢乙啶染色观察血管病理改变;采用RT-qPCR和Western blot方法检测血管组织中衰老标志物、昼夜节律因子和核因子κB(nuclear factor-κB.NF-κB)/核苷酸结合寡聚化结构域样受体蛋白3(nucleotide-binding oligomerization domain-like receptor protein 3,NLPR3)通路关键因子的表达水平。(2)体外培养人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC),用过氧化氢(hydrogen peroxide,H_(2)O_(2))诱导建立衰老细胞模型,并用REV-ERBs激动剂SR9009干预,检测衰老标志物、REV-ERBs及NF-κB/NLPR3通路关键因子的表达。结果:与青年组相比,老年组小鼠体重显著升高,骨骼肌、脾、胸腺重量及其指数显著下降(P<0.05)。老年组小鼠血管中膜厚度增加,纤维化面积、脂质沉积、SA-β-Gal阳性面积、活性氧(reactive oxygen species,ROS)含量和CD68表达均显著升高(P<0.05);老年组小鼠血管组织REV-ERBs的mRNA和蛋白水平显著上调(P<0.05),磷酸化NF-κB和NLRP3蛋白水平,以及白细胞介素1β(interleukin-1β,IL-1β)、IL-6和肿瘤坏死因子α的mRNA表达水平也显著升高(P<0.05)。H_(2)O_(2)可诱导HUVEC衰老,SR9009能显著降低衰老细胞SA-β-Gal阳性率和ROS含量,抑制NF-κB/NLRP3炎症通路相关因子表达(P<0.05)。结论:REV-ERBs可能通过调控NF-κB/NLRP3炎症通路激活,促进血管内皮细胞炎症,进而参与衰老小鼠血管重塑过程。展开更多
Published:18 July 2025 The published article titled“Knockdown of REV7 Inhibits Breast Cancer Cell Migration and Invasion”has been retracted from Oncology Research,Vol.24,No.5,2016,pp.315–325.DOI:10.3727/096504016X1...Published:18 July 2025 The published article titled“Knockdown of REV7 Inhibits Breast Cancer Cell Migration and Invasion”has been retracted from Oncology Research,Vol.24,No.5,2016,pp.315–325.DOI:10.3727/096504016X14666990347590 URL:https://www.techscience.com/or/v24n5/56980.Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells.In addition,the western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.展开更多
文摘Published:18 July 2025 The published article titled“Knockdown of REV7 Inhibits Breast Cancer Cell Migration and Invasion”has been retracted from Oncology Research,Vol.24,No.5,2016,pp.315–325.DOI:10.3727/096504016X14666990347590 URL:https://www.techscience.com/or/v24n5/56980.Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells.In addition,the western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases.
